DNASU Plasmid | Asb14 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Asb14

Gene Name: ankyrin repeat and SOCS box-containing 14

Original Clone ID:

FLH270139.01L

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00297276

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1458nts

Open reading frame: 1 to 1458

ATGAACGCTTCCGAACCCAGTACGTGGGAACGAACCACTCACAACGGTGAAACAGCACTT
TTTTTGGCTGTCAGCAGTAGCCTCCTGGAAAACGCCCACTTTCTCCTTCTCAAAGGCTGT
AATCCAAACGCTAAAACTTCTGAAGGCAATTCTCCTCTTCTTACAGCTGTTCTGAAAGAC
GCCTACGACATGGCCACCTTGCTGATCAGCCATGGAGCAGATGTCAATCTCCGATGTGCC
AATGAGAGGACAGCTCTCCATGAAGCTGCCAAGCTGGGCAGACTGGACATGGTAAAACTG
ATGCTGGCTTCTGGAGCATACCCAGATGCACGGAGCTCCTACGGATTCACGCCCCTTGCC
CTCGCTGCTCAAGGTGGACACACTGGGATCATGCAACTACTACTGCAGAAAGGAGCTGAC
GTTCACAGTCAGGCCTCTGACTCGTCCTCTGTGTTACTTGAAGCTGTTAGAGGAGGGAAT
CCGGAAGCTGTGAGTCTGTTGTTAGAGTATGGAGCTGATGCCAACATTCCTAAGAGTTCA
GGCCACCTGCCCATCCACGTGGCAGCTGACAAAGGCCATTTCCTAGCTCTAAAGGTATTG
GTCCCAGTCACAGACATCGCAGCCATCAAGAAGAGCGGGATCAGTCCGGTTCACTGCGCA
GCAGCTGGGGCGCACCCCCACTGCCTGGAACTTCTCATTCAGGCTGGATTTGATGTGAAT
TTCATGCTGGATCAGAGAATCCGTAAACATTATGATGATCAACGGAAGTCAGCCTTGTAT
TTTGCTGTTTCAAACGGTGACCTCCCTTCAGTTAAGCTGCTTCTGAGTGCTGGAGCTCTA
CCCAATCAAGACCCAGTCAACTGCCTACAGATAGCTCTTAGGATGGGCAACTATGAGCTC
ATAAGTCTATTACTGAGGCACGGGGCCAACGTCAACTACTTCTGCCGCGTTAACCCTTTG
CATTTCCCATCAGCGCTACAATACACATTGAAAGATGAAGTCATGCTCAGGATGCTGTTG
AATTATGGCTATGACACTGAGCGATGTTTTGATTGTCCTCATGGAGAAAGGGTTCATCGG
TTTTGTACTTTTGAAGGCTGGACATCTACAGTTATTAAAGATACTATGTTTTGTGAAGTC
ATAACTTTGTCATGGCTACAGCATCTCTCTGGGAAAGTTGTTCGTGTGATGCTTGATTAT
GTTGATCAAGTTCAAATCTGTTCAAAATTGAAAGCTGTGCTGGAAAAACAAAGGCTCTGG
CCAGAAATACATTTTATCTTGACCAACCCCCGTTCTCTACAACATTTGTGCCGCCTTAAG
ATCCGGAAGTGTATGGGACGGTTACGTTTGCGCTGCCCCGTCTTCATGTCGTTTCTTCCG
CTGCCCAATCTCTTAAAAGCGTACGTCCTTTACAAAGAATATGACCTTTTTGGACAAGAA
CGTTCTACAGGAACATGG

Protein sequence predicted based on nucleotide sequence

MNASEPSTWERTTHNGETALFLAVSSSLLENAHFLLLKGCNPNAKTSEGNSPLLTAVLKD
AYDMATLLISHGADVNLRCANERTALHEAAKLGRLDMVKLMLASGAYPDARSSYGFTPLA
LAAQGGHTGIMQLLLQKGADVHSQASDSSSVLLEAVRGGNPEAVSLLLEYGADANIPKSS
GHLPIHVAADKGHFLALKVLVPVTDIAAIKKSGISPVHCAAAGAHPHCLELLIQAGFDVN
FMLDQRIRKHYDDQRKSALYFAVSNGDLPSVKLLLSAGALPNQDPVNCLQIALRMGNYEL
ISLLLRHGANVNYFCRVNPLHFPSALQYTLKDEVMLRMLLNYGYDTERCFDCPHGERVHR
FCTFEGWTSTVIKDTMFCEVITLSWLQHLSGKVVRVMLDYVDQVQICSKLKAVLEKQRLW
PEIHFILTNPRSLQHLCRLKIRKCMGRLRLRCPVFMSFLPLPNLLKAYVLYKEYDLFGQE
RSTGTW

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1899

Start on reference sequence 439

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Asb14

Symbol Nomenclature :

Asb14

SYNONYM :

Asb-14

Designation :

ankyrin repeat and SOCS box protein 14|ankyrin repeat and SOCS box-containing protein 14|ankyrin repeat domain-containing SOCS box protein 14

Full Nomenclature :

ankyrin repeat and SOCS box-containing 14

GENEID :

142687

Mouse Genome Informatics :

2655107

Vega :

OTTMUSG00000035287

Target GenBank:

NM_080856.2

Protein

SMART domain :

SOCS : suppressors of cytokine signalling

SMART domain :

SOCS_box :

SMART domain :

ANK : ankyrin repeats

UniProt :

Q8C6Y6

UniProt :

E9QPD0

Clone

Cloning Information :

270139

HIP Master Clone ID :

201641

IMAGE ID :

100058994

Original Clone ID :

FLH270139.01L

Organism

Species Specific ID:

142687

Genome

Chromosome :

Map Location :

14 A3|14

Ensembl :

ENSMUSG00000021898

Reagents

Labome :

Asb14-antibody