Recql5 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Recql5
Gene Name:  RecQ protein-like 5
Original Clone ID:
FLH270167.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297304
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2946nts
Open reading frame: 1 to 2946

ATGAGCGCCCGTCCTTTCTCCACTCCCTTCGACCGGGAGCGGCGAGTCCGGAGCACCCTG
AAGAAAGTCTTTGGGTTTGATTCTTTTAAAACACCTTTACAAGAGAGTGCGACCATGGCT
GTAGTGAAAGGTGCCGAGGATGTGTTTGTGTGCATGCCCACAGGGGCAGGGAAGTCTCTG
TGCTATCAACTCCCTGCCCTGTTGGCCAGTGGGATCACCATTGTAGTCTCTCCTCTCATT
GCCTTGATTCAGGACCAGGTAGACCACTTGCTGGCCTTGAAGGTACAAGTTAGTTCCCTG
AACTCAAAGCTCTCGGTACAGGAGAGGAAGGAGCTGCTGTCTGACCTGGAGCGAGACAAA
CCTCGGACCAAACTTTTGTACATCACCCCAGAGATGGCAGCTTCAGCCTCCTTCCAGCCC
ACCCTGAATTCCCTGGTGTCCCGAAACCTGCTCTCCTACTTGGTGGTGGATGAAGCTCAT
TGCGTTTCCCAGTGGGGACATGACTTCCGTCCTGACTATTTGCGTCTGGGTGCCCTACGT
TCCCGCTTGGCACATGCCCCGTGCGTGGCTCTGACGGCTACAGCCACCCCACAGGTTCAA
GAGGATGTGTTTGCTGCCCTGCACTTGAAGCAGCCAGTCGCCTCTTTCAAGACTCCCTGT
TTCCGGGCCAACCTCTTCTATGATGTGCAGTTCAAGGAACTGATTCCCGACGTCTATGGC
AATCTGAGGGACTTCTGCCTTAAGGCTCTTGGACAGAAGGCTGAGAACGGGTCGTCCTCT
GGCTGTGGCATTGTCTACTGCAGGACTAGAGAAGCTTGTGAACAACTGGCCATTGAGCTC
AGCAGCAGGGGTGTGAACGCCAAGGCTTACCACGCAGGCCTGAAGGCATCTGATAGAACA
CAGGTACAGAACGAGTGGATGGAGGAGAAGGTCCCTGTAATTGTTGCGACCATCAGTTTT
GGGATGGGAGTAGACAAAGCTAATGTCCGGTTTGTTGCTCATTGGAATATTGCCAAGTCC
ATGGCTGGCTACTACCAGGAGTCTGGCCGTGCTGGCAGGGATGGCAAACCATCTTGGTGC
CGCCTGTATTACTCTAGGAATGACCGAGACCAAGTCAGCTTCCTTATCAGGAAGGAACTA
GCCAAACTCCAGGAAAAGAGGGGGAACAAACCGTCTGATAAGGCCACCCTGCTGGCCTTT
GATGCCCTGGTGACCTTCTGTGAAGAAGTGGGGTGCCGCCATGCTGCCATTGCTAAGTAC
TTTGGGGATGCACCCCCCGCCTGCGCCAAAGGCTGTGACTACTGCCAGAATCCTGCAGCC
ATAACGAAGAAGCTGGATGCCCTGGAGCGCAGCAGCAGCTGGAGCAAGACCTGCATCGGG
CCCTCACAGGGGAATGGCTTCGATCCTGAGCTGTATGAGGGAGGCCGCAGGGGCTATGGT
GGCTTCAGCAGGTATGATGAAGGTTCTGGAGGTAGCGGTGATGAGGGCAGAGATGAGGCC
CACAAGCGGGAATGGAATCTTTTCTATCAGAAACAGATGAGCCTGCGCAAGGGCAAAGAG
CCCAAGATAGAAGAATTCACGCCCCCAGATGAAGACTGTCCCCTGAGAGAGGCTTCAAGC
AGGAAGATCCCGAAGCTCACAGTGAAGGCCCGTGAGCACTGCCTGCGACTTCTGGAGGAG
GCTCTGATCAGTAACCATCAGGCTGCAGGCTCCACTCATGGAGCTGACCTGCAGGCCAAG
GCTGTGGAACTGGAGCATGAGACATTCCGGAATGCCAAGATGGTCAATCTGTACAAGGCC
AGTGTCCTGAAGAAGGTGGCCGAGATCCACAAAGCCTCCAAGGATGGTCAGCTCTATGAC
ATGGAAAGCGGCACCAAGAGCTGTGGGGCCGCAGCTGAGTTCTCGGAGCCCAGTGACTAT
GACATCCCGCCAACCTCACATGTGTACTCGCTCAAACCCAAGAGAGTAGGAGCTGGCTTC
TCCAAAGGCCCCTGCTCATTCCAGACAGCCACAGAACTGTTGGGGAAGTCTCACTCCCAG
AAGCAAGCCCCAGAGGCTATGCTAGAGGGTGGACAGGAGCCCCCAGGCTGGGTCTGTGAC
CTTCAAGATGAAGACAGGAGCAAGCCCCACCCTGGGTACCAAGAAAAGGCTCTAGGAAGC
AGTGTTAATTGTGGGGACCCCTCGCCTGAGAAGAAGACGAAAGGGTCTTCCCAGGGCAGT
GCCAAAGCTAGAGCCAGCAAGAAACAACAGCTCCTAGCCACAGCAGCCCGCAAGGATTCC
CAGAATATCACTCGATTCCTCTGTCAGAGGACAGAATCTCCACCTCTCCCTGCTTCAGTT
CCAAGATCAGAAGATGCCAGCCCCTCCTGTGGGGATGTCCCAGGGAAGTGCACACAAGAG
GTTGGAGCTCAGGGACATTTGGTTGCTGTATTTCAGACTGAGGGTCCCAGGGAGAGGCCA
AGCACCTGCTCACTCAGAGACCAGAGCTTCCCTGAAGGTCAGCCTAGCCCTCTAAAGGAA
ACCCAGGCAGAGAAGCGGCCCAGACCACAGCAGGGCAACCCAGAGAGAAGGGCTCAAAAG
AGGCTTCGCCCCTCAACCAAGTCCTCCATCTTGGCTGAGGCCAAGGACAGTACCTTGGCC
AGTGATCGCAGCACTGAAAACAAAGTGGCTCAAGAACCCTGCCAGCTCTCAGCATCTGGC
ACCTCCCTGAGGGAGGCCGCAGACATTGTGGTCAGGCACCTGACCCCCTTCTACAAGGAG
GGCAGGTTTATATCCAAGGACCTGTTTAAGGGCTTTGCCCGCCACCTCTCACATTTGCTG
GCTCAGCAGCTCTCTCCTGGAAGGAGTGTGAAGGAAGAGGCCCAGAGCCTCATCAAGCAG
TTCTTCCACAATCGTGCCCGCTGTGAGAGTGAAGCTGACTGGCACAGCCTGCGTGGCCCA
CAGCGA

Protein sequence predicted based on nucleotide sequence

MSARPFSTPFDRERRVRSTLKKVFGFDSFKTPLQESATMAVVKGAEDVFVCMPTGAGKSL
CYQLPALLASGITIVVSPLIALIQDQVDHLLALKVQVSSLNSKLSVQERKELLSDLERDK
PRTKLLYITPEMAASASFQPTLNSLVSRNLLSYLVVDEAHCVSQWGHDFRPDYLRLGALR
SRLAHAPCVALTATATPQVQEDVFAALHLKQPVASFKTPCFRANLFYDVQFKELIPDVYG
NLRDFCLKALGQKAENGSSSGCGIVYCRTREACEQLAIELSSRGVNAKAYHAGLKASDRT
QVQNEWMEEKVPVIVATISFGMGVDKANVRFVAHWNIAKSMAGYYQESGRAGRDGKPSWC
RLYYSRNDRDQVSFLIRKELAKLQEKRGNKPSDKATLLAFDALVTFCEEVGCRHAAIAKY
FGDAPPACAKGCDYCQNPAAITKKLDALERSSSWSKTCIGPSQGNGFDPELYEGGRRGYG
GFSRYDEGSGGSGDEGRDEAHKREWNLFYQKQMSLRKGKEPKIEEFTPPDEDCPLREASS
RKIPKLTVKAREHCLRLLEEALISNHQAAGSTHGADLQAKAVELEHETFRNAKMVNLYKA
SVLKKVAEIHKASKDGQLYDMESGTKSCGAAAEFSEPSDYDIPPTSHVYSLKPKRVGAGF
SKGPCSFQTATELLGKSHSQKQAPEAMLEGGQEPPGWVCDLQDEDRSKPHPGYQEKALGS
SVNCGDPSPEKKTKGSSQGSAKARASKKQQLLATAARKDSQNITRFLCQRTESPPLPASV
PRSEDASPSCGDVPGKCTQEVGAQGHLVAVFQTEGPRERPSTCSLRDQSFPEGQPSPLKE
TQAEKRPRPQQGNPERRAQKRLRPSTKSSILAEAKDSTLASDRSTENKVAQEPCQLSASG
TSLREAADIVVRHLTPFYKEGRFISKDLFKGFARHLSHLLAQQLSPGRSVKEEAQSLIKQ
FFHNRARCESEADWHSLRGPQR

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3037
Start on reference sequence 89
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable