Gene:
Gene Symbol:
Lgr4
Gene Name:
leucine-rich repeat-containing G protein-coupled receptor 4
Original Clone ID:
FLH481709.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297390
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2856nts
Open reading frame: 35 to 2890
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCCGGGCCCGCTGCGGCTGCTCTG
CTTCTTCGCCCTGGGGCTGCTCGGCTCGGCCGGGCCCAGCGGCGCGGCGCCACCTCTCTG
CGCTGCGCCCTGCAGCTGCGACGGCGACCGTCGGGTGGACTGCTCCGGGAAGGGGTTGAC
GGCGGTACCGGAGGGGCTCAGCGCCTTCACCCAAGCACTGGATATCAGTATGAACAATAT
CACTCAGTTACCAGAAGATGCATTTAAGAATTTTCCTTTTCTAGAGGAGCTACAACTGGC
TGGTAACGACCTTTCTTTTATCCACCCAAAAGCCTTGTCTGGGTTGAAAGAACTCAAAGT
CCTAACCCTCCAGAACAATCAGTTGAAAACAGTACCCAGTGAAGCCATTCGTGGACTGAG
TGCTTTGCAGTCTCTACGCTTAGATGCCAACCATATTACCTCAGTCCCGGAGGACAGTTT
TGAAGGGCTCGTTCAGTTGCGGCATCTGTGGCTGGATGACAACATCTTGACGGAAGTGCC
TGTGCGTCCGCTCAGCAACCTGCCAACCCTGCAGGCGCTGACCTTGGCTCTCAACAACAT
CTCAAGCATCCCCGACTTCGCATTCACCAACCTTTCAAGCTTGGTAGTGCTGCATCTTCA
TAACAATAAAATTAAAAGCCTCAGTCAACACTGTTTTGATGGACTAGATAACCTGGAAAC
CCTGGACTTGAATTATAATAACTTGGATGAATTTCCTCAGGCTATTAAAGCCCTTCCCAG
CCTTAAAGAGCTGGGATTTCACAGTAATTCTATTTCTGTTATCCCGGATGGAGCATTTGC
TGGTAATCCACTGCTAAGAACTATCCATTTGTATGATAATCCTCTGTCTTTTGTGGGGAA
CTCAGCATTTCACAACCTGTCTGATCTGCATTCCTTAGTCATTCGTGGTGCAAGCCTGGT
GCAGTGGTTCCCCAATCTGGCCGGAACTGTCCATCTGGAGAGTCTAACCTTGACAGGGAC
AAAAATAAGCAGCATACCTGATGATCTGTGCCAAAACCAAAAGATGCTGCGGACTCTGGA
CTTATCTTATAACGATATAAGAGACCTTCCAAGTTTTAATGGTTGTCGTGCATTGGAAGA
AATTTCATTGCAGCGTAATCAAATCTCCCTGATAAAGGAAACTACTTTTCAAGGCCTAAC
ATCCCTAAGGATTCTAGATCTGAGTAGAAACCTGATTCGTGAAATTCACAGTGGAGCTTT
TGCGAAGCTTGGGACAATTACTAACCTGGATGTGAGTTTCAATGAATTAACCTCATTTCC
TACGGAAGGCCTGAATGGGCTCAATCAACTTAAACTTGTGGGTAACTTCCAGCTGAAAGA
TGCCTTGGCAGCCAGAGACTTTGCCAATCTCAGGTCTCTATCAGTACCATATGCTTATCA
GTGTTGTGCATTTTGGGGGTGTGACTCTTATGCAAATTTAAACACAGAAGATAACAGCCC
CCAAGACCACAGTGTGACAAAAGAGAAAGGTGCTACAGATGCAGCAAATGCCACCAGCAC
TGCTGAAAGTGAAGAACATAGCCAAATAATCATCCATTGTACACCTTCAACAGGTGCTTT
TAAGCCCTGTGAATATTTACTGGGAAGCTGGATGATTCGCCTTACAGTGTGGTTCATTTT
CCTGGTCGCCTTGCTTTTCAACCTGCTTGTCATTTTAACAGTGTTTGCGTCTTGTTCATC
ACTGCCTGCCTCCAAGCTCTTCATAGGCTTGATTTCTGTGTCTAACTTACTCATGGGCAT
CTATACTGGCATCCTTACTTTTCTTGATGCTGTGTCCTGGGGCCGATTTGCTGAATTTGG
CATTTGGTGGGAAACTGGCAGCGGCTGCAAGGTAGCTGGGTCTCTGGCAGTCTTCTCCTC
AGAGAGCGCCGTATTCCTGTTAACCCTGGCAGCCGTGGAAAGAAGCGTTTTTGCAAAGGA
TGTAATGAAAAATGGGAAAAGCAGTCACCTCCGACAGTTCCAGGTGGCTGCCCTCGTAGC
TTTGCTGGGTGCTGCAATAGCAGGCTGCTTCCCCCTTTTTCACGGAGGGCAATATTCTGC
ATCACCCTTGTGCTTGCCATTTCCTACAGGAGAGACACCATCATTAGGATTCACTGTGAC
CCTAGTGCTATTAAACTCACTAGCATTTTTATTGATGGCCATTATCTACACTAAACTCTA
CTGCAACTTAGAGAAAGAAGACCCGTCAGAAAACTCCCAGTCTAGCATGATTAAGCACGT
TGCTTGGCTCATCTTCACAAACTGCATCTTCTTCTGCCCTGTTGCATTTTTCTCATTCGC
ACCATTGATCACGGCAATCTCCATCAGCCCCGAGATAATGAAGTCTGTTACGCTGATATT
CTTCCCGCTGCCTGCTTGCCTGAATCCAGTCCTGTACGTTTTCTTCAACCCAAAGTTTAA
AGACGACTGGAAGCTCCTGAAGCGGCGTGTCACCAGGAAACACGGATCAGTCTCAGTCTC
CATCAGCAGCCAAGGCGGTTGTGGGGAACAGGATTTCTACTACGACTGTGGCATGTATTC
CCACTTGCAGGGTAACCTGACTGTCTGTGACTGCTGTGAGTCATTTCTTCTGACAAAACC
AGTATCGTGCAAACACTTAATAAAATCGCACAGTTGTCCTGTATTGACAGTGGCCTCTTG
CCAGAGGCCAGAGGCCTACTGGTCTGATTGTGGCACACAGTCGGCCCATTCTGACTATGC
AGATGAAGAGGATTCCTTTGTCTCGGACAGCTCTGACCAGGTGCAGGCCTGTGGACGAGC
CTGCTTCTACCAGAGTCGCGGATTCCCTCTGGTGCGCTATGCTTATAATCTACCGAGAGT
CAGAGACTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MPGPLRLLCFFALGLLGSAGPSGAAPPLCAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQ ALDISMNNITQLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTV PSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNILTEVPVRPLSNLPTLQ ALTLALNNISSIPDFAFTNLSSLVVLHLHNNKIKSLSQHCFDGLDNLETLDLNYNNLDEF PQAIKALPSLKELGFHSNSISVIPDGAFAGNPLLRTIHLYDNPLSFVGNSAFHNLSDLHS LVIRGASLVQWFPNLAGTVHLESLTLTGTKISSIPDDLCQNQKMLRTLDLSYNDIRDLPS FNGCRALEEISLQRNQISLIKETTFQGLTSLRILDLSRNLIREIHSGAFAKLGTITNLDV SFNELTSFPTEGLNGLNQLKLVGNFQLKDALAARDFANLRSLSVPYAYQCCAFWGCDSYA NLNTEDNSPQDHSVTKEKGATDAANATSTAESEEHSQIIIHCTPSTGAFKPCEYLLGSWM IRLTVWFIFLVALLFNLLVILTVFASCSSLPASKLFIGLISVSNLLMGIYTGILTFLDAV SWGRFAEFGIWWETGSGCKVAGSLAVFSSESAVFLLTLAAVERSVFAKDVMKNGKSSHLR QFQVAALVALLGAAIAGCFPLFHGGQYSASPLCLPFPTGETPSLGFTVTLVLLNSLAFLL MAIIYTKLYCNLEKEDPSENSQSSMIKHVAWLIFTNCIFFCPVAFFSFAPLITAISISPE IMKSVTLIFFPLPACLNPVLYVFFNPKFKDDWKLLKRRVTRKHGSVSVSISSQGGCGEQD FYYDCGMYSHLQGNLTVCDCCESFLLTKPVSCKHLIKSHSCPVLTVASCQRPEAYWSDCG TQSAHSDYADEEDSFVSDSSDQVQACGRACFYQSRGFPLVRYAYNLPRVRD*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3330
Start on reference sequence
475
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
