Gene:
Gene Symbol:
Scaper
Gene Name:
S phase cyclin A-associated protein in the ER
Original Clone ID:
FLH481797.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297478
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4197nts
Open reading frame: 35 to 4231
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGATGGCTTCCTTCCAGCGCTCCAA
TAGTCATGACAAAGTGAGAAGAATTGTTGCTGAGGAGGGTCGTACCGCAAGAAACTTGAT
AGCTTGGAGTGTTCCACTAGAAAGCAAAGATGATGATGGAAAACCGAAATGTCAAACTGG
TGGAAAATCTAAGAGGACAATTCAAGGCACTCATAAAACCACTAAACAGAATACTGCAGT
AGAATGTAAAATAACATCCACAACTGGAGAGAAGCATTTTGATAAAAGTCCTACAAAAAC
AAGGCACCCAAGAAAAATTGACTTAAGAGCTCGCTACTGGGCATTTCTTTTTGACAATCT
TCGCCGGGCAGTAGATGAAATCTATGTGACTTGTGAATCAGATCAGAGTGTGGTGGAGTG
TAAGGAGGTGTTAATGATGCTGGATTACTATGTGAGAGATTTCAAAGCATTGATTGACTG
GATTCAACTTCAGGAAAAGTTAGAGAAGACAGATGCTCAAAGCAGACCAACCTCACTGGC
GTGGGAAGTAAAGAAGATGTCTCCAGGACGTCATGTGATTCAAAGCCCATCAACAGATAG
AATGAATGTAACATCAACTGCAAGAAGAAGCTTAAACTTTGGGAGTTTACCTGGTGCAGT
AACTGCCCCATGTCTGGCTCCCACAGGTGTCAGTTGGGCTGACAAGGTAAAGGCTCATCA
TACTGGTTCTGCTCCTCCTGAGGCCACACCTGCTCAATCCTGTGCACCAATGACAGTGCA
AAAGACGTCCCGGAAAAATGAACGGAAAGATGCTGAGGGTTGGGAGACTGTTCAGAGAGG
TCGGCCAGTGCGTTCCCGATCGACAGCAGTTATACCTAAAGTTTCACTAGTGACAGAAAC
CTTAAGGTCAAAGGATGACAGTGATAAGGAAAACATATGCCTTTTACCTGAAGAAAGCAT
ACAGAAAGGAAAACTTGAAGACAGATGTTCTACTGTTGAATCGTTATCCAAAGACTCATT
ATTACATTCTTCTGACCATCCTCTTTCTGAGAGAACTCAGTTCACAGTGTGTATATTGGA
TGATGTCAAGAATTCTGGTAGTAGTCAAGACAGTTCAGTGCATACTTCTGAAATACCAGC
TATTGACAGTGATGCAGTATGTGTTGCTCCACAAAACACCACAGCTTTGCCAACAGACAG
AGTTCCAGCGGAGACAACAGGAATGGTGACTGAGATGACGGACCCTTCAGACATTTCAAA
TGTGAGTGCTGCTGACTGGTCTATGGCAGAAGTTCTCGCTAAAAAAGAAGAGCTAGCAGA
TCGTCTAGAAAAGGCCAATGAAGAAGCCATCGCCAGTGCTATTGCTGAAGAAGAACAATT
AACCAGAGAAATTGAAGCTGAAGAAAACAATGACATTAACATCGAGACTGACAACGACAG
TGACTTTTCTGCCAGTATGGGCAGTGGGAGTCTATCTTTCTGTGGTGTGTCTCTGGACTG
GAATGATGTTCTCGCAGATTATGAAGCTCGTGAATCATGGCGTCAAAATACATCCTGGGG
AGATATTGTAGAAGAGGAGCCTGCCAGACTTCCAGGGCATGGAATTCACATGCATGAAAA
ACTTTCATCACCTTCTCGTAAAAGGACAATCGCAGAATCTAAAAAGAAATATGAAGAAAA
ACACATGAAAGCTCAACAGTTAAGGGAAAAGCTACGCGAAGAGAAGACACTAAAGCTTCA
AAAGTTGTTAGAAAGGGAGAAAGATGTCCGAAAGTGGAAGGAAGAATTACTAGATCAACG
ACGTAGGATGATGGAAGAGAAATTACTTCATGCAGAATTTAAACGAGAGGTACAGCTACA
AGCTATTGTGAAGAAAGCTCAAGAGGAAGAAGCTAAGGTTAATGAGATTGCCTTCATAAA
TACCCTTGAAGCTCAGAATAAACGTCACGATGTTTTATCAAAACTGAAGGAATATGAGCA
GAGGCTTAATGAATTGCAGGAAGAACGTCAGAGAAGACAAGAAGAAAAGCAAGCTCGTGA
TGAGGCTGTGCAGGAACGTAAGAGAGCCTTGGAGGCAGAAAGGCAGGCCCGTGTAGAGGA
ATTATTGATGAAGAGGAAAGAGCAAGAAGCCCGAATTGAGCAACAGAGGCAAGAGAAGGA
GAAAGCTCGTGAAGATGCAGCCCGGGAAAGAGCAAGAGACAGGGAAGAACGGTTGGCAGC
GCTCACAGCAGCTCAGCAAGAAGCTATGGAAGAGCTGCAGAAAAAAATTCAGCTCAAGCA
TGATGAAAGCATTCGAAGGCACATGGAACAGATTGAGCAAAGGAAAGAAAAAGCTGCTGA
ATTAAGCAGTGGGCGGCATGCAAGCACCGATTACGCCCCTAAACTGACCCCTTATGAAAG
AAAGAAGCAGTGTTCTCTCTGCAATGTCCTGATCGCTTCTGAAGTATATCTCTTTAGCCA
CATTAAAGGGAAAAAACATCAGCAGGCTGTGAGAGAGAATAGCAGTATCCAGGGCCGTGA
ACTTTCTGATGAGGAAGTGGAACATCTGTCTTTGAAGAAGTATGTTGTTGACATTGTGAT
TGAAAGTGCAGCTCCACCAGAGCCTGTAAAAGATGGAGAAGAACGGCAAAAGAATAAGAA
AAAAGCTAAAAAGATAAAAGCACGGATGAACATCAGGGCGAAAGAATATGAGAATTTAGT
GGAAACCAAAAATTCTGGCTCCGAGTCACCTTATAAAGCAAAGCTTCAGAGATTAACCAA
AGATCTTGTAAAACAACTTCAAGTACAAGACAGTGGCTCTTGGGTAAATAATAAGGCTTC
AGCTTTGGATCGGACCCTTGGTGAGATTGCTAGAATATTGGAGAAAGAGAATGTGCCAGA
TCAGATTTCGTTTCAAGTTGCTGGTGGATTAACAGCCCTTGAAAACATCCTTCAAGTAGT
GACTCCAGCCACAAACATGAACACGGTTTCACGAATTCCTCCTAAGTCTCTCTGCAATGC
AATCAATGTTTACAGCCTCGCCTGCAATAACTGCTCAGAAAACTGCACTGATGTTCTGTT
TAGTAACAAGATTACCTTCTTAATGGACCTCCTGATACACCAATTGACGGTTTATGTTCC
AGATGAAAATAATGCTGTTCTAGGAAGAAATACAAATAAACAAGTTTTTGAAGGCTTGAC
GACTGGACTTCTCAGAGCTGGTGCTGTGGTTTTTAGCTGTCTGATTGCCAACCGACCAGA
TGGAAACAGCCGTCCAGCTACACCAAAAATATCAACACAGGAAATGAAAAACAAACCCTC
ACAAGGTGATGCTTTTAACAGTCGAGTGCAAGACCTTATCAGCTATGTGGTGAACATGGG
TCTGATTGACAGACTGCATGGCTGCTTCCTCTCAGTGCAAGGCCCAGTGGATGAGAATCC
CAAAATGGCCACATTTCTGCAGCACGCCGCAGGCTTCCTACGTGGGATGTGTATGCTATG
CTTTGCTGTCACTGGAAGGTCCTCCAGCATATTTGACAATAATCACCAGGATCCCACAGG
GCTGACAGCTGCTCTTCAGGCCACAGACTTGGCTGGAGTTCTACATATGCTCTACTGTAT
CCTCTTCCACGGCACCATCTCAGACGCCGGCACGAGTCCCAAGGACAGTTACACCCAGAA
CACCATCCAAGTAGCCATTCAGAGTTTACGTTTCTTCAATAGCTTTGCAATTCTTGATTT
GTCTGCTTTTCAGTCTGTTGTAGGGGCAGAGGGCTTGTCCCTTGCATTCCGGCACATGGC
TAGCTCCTTGCTAGGCCACTGCAGCCAAGTGTCCTGTGAAAGCCTCCTCCATGAGGTCAT
CGTCTGTGTGGGCTACTTCACTGTCAACCACCCAGATAACCAGGTGATTGTTCAGTCCGG
CCGCCACCCAACGGTGCTGCAGAAGCTCTGCCAGCTGCCTTTCCAGTATTTCAGTGACCC
GAGGCTCATCAAAGTGCTGTTTCCTTCGCTTATCACCGCGTGTCACAACAACCATCAGAA
CAAGCTCATTCTGGAGCAAGAGATGAGCTGTGTTTTACTGGCCACATTCATCCAGGACTT
TGCACAGACTCCGGGTCACGTGGATAACCAGTCTTCAAGTCTCAAAGGAAAATTCCAGAA
CCCCGGGAACTACCTGGAACTGGCTAACAGATTTCCTCAGCAGGCCTGGGAAGAAGCCCG
GCAATTTTTCTTGAAAAAAGAAAAAAAATAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MMASFQRSNSHDKVRRIVAEEGRTARNLIAWSVPLESKDDDGKPKCQTGGKSKRTIQGTH KTTKQNTAVECKITSTTGEKHFDKSPTKTRHPRKIDLRARYWAFLFDNLRRAVDEIYVTC ESDQSVVECKEVLMMLDYYVRDFKALIDWIQLQEKLEKTDAQSRPTSLAWEVKKMSPGRH VIQSPSTDRMNVTSTARRSLNFGSLPGAVTAPCLAPTGVSWADKVKAHHTGSAPPEATPA QSCAPMTVQKTSRKNERKDAEGWETVQRGRPVRSRSTAVIPKVSLVTETLRSKDDSDKEN ICLLPEESIQKGKLEDRCSTVESLSKDSLLHSSDHPLSERTQFTVCILDDVKNSGSSQDS SVHTSEIPAIDSDAVCVAPQNTTALPTDRVPAETTGMVTEMTDPSDISNVSAADWSMAEV LAKKEELADRLEKANEEAIASAIAEEEQLTREIEAEENNDINIETDNDSDFSASMGSGSL SFCGVSLDWNDVLADYEARESWRQNTSWGDIVEEEPARLPGHGIHMHEKLSSPSRKRTIA ESKKKYEEKHMKAQQLREKLREEKTLKLQKLLEREKDVRKWKEELLDQRRRMMEEKLLHA EFKREVQLQAIVKKAQEEEAKVNEIAFINTLEAQNKRHDVLSKLKEYEQRLNELQEERQR RQEEKQARDEAVQERKRALEAERQARVEELLMKRKEQEARIEQQRQEKEKAREDAARERA RDREERLAALTAAQQEAMEELQKKIQLKHDESIRRHMEQIEQRKEKAAELSSGRHASTDY APKLTPYERKKQCSLCNVLIASEVYLFSHIKGKKHQQAVRENSSIQGRELSDEEVEHLSL KKYVVDIVIESAAPPEPVKDGEERQKNKKKAKKIKARMNIRAKEYENLVETKNSGSESPY KAKLQRLTKDLVKQLQVQDSGSWVNNKASALDRTLGEIARILEKENVPDQISFQVAGGLT ALENILQVVTPATNMNTVSRIPPKSLCNAINVYSLACNNCSENCTDVLFSNKITFLMDLL IHQLTVYVPDENNAVLGRNTNKQVFEGLTTGLLRAGAVVFSCLIANRPDGNSRPATPKIS TQEMKNKPSQGDAFNSRVQDLISYVVNMGLIDRLHGCFLSVQGPVDENPKMATFLQHAAG FLRGMCMLCFAVTGRSSSIFDNNHQDPTGLTAALQATDLAGVLHMLYCILFHGTISDAGT SPKDSYTQNTIQVAIQSLRFFNSFAILDLSAFQSVVGAEGLSLAFRHMASSLLGHCSQVS CESLLHEVIVCVGYFTVNHPDNQVIVQSGRHPTVLQKLCQLPFQYFSDPRLIKVLFPSLI TACHNNHQNKLILEQEMSCVLLATFIQDFAQTPGHVDNQSSSLKGKFQNPGNYLELANRF PQQAWEEARQFFLKKEKK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
178
End on reference sequence
4374
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
