Gene:
Gene Symbol:
No insert
Gene Name:
No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pSGP46
Source:
Center for High-Throughput Structural Biology
Description:
Yeast expression vector with Adh2 promoter, adds C-terminal 3C-ZZ-10XHis tag; ampicillin resistance (bacteria); uracil resistance (yeast); ligation independent cloning (LIC).
Comments:
None
Publications:
PMID: 20045057
Title: Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography
Title: Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography
Authors:
Mark Dumont
Kathleen Clark
Center for High Throughput Structural Biology
PSI
Kathleen Clark
Center for High Throughput Structural Biology
PSI
EvNO00304416
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
URA
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Vector Name:
pSGP46
Synonyms:
''
Sequencing Primer:
Forward:
pSGP-Forward
Reverse:
pSGP-Reverse
Description:
Yeast expression vector with Adh2 promoter, adds C-terminal 3C-ZZ-10XHis tag; ampicillin resistance (bacteria); uracil resistance (yeast); ligation independent cloning (LIC).
Comments:
S. cerevisiae expression strain
Size (bp):
6675
Parent Vector:
None
Properties:
ligation independent cloning (LIC), with tag/fusion/marker, yeast expression
Author Name:
Mark Dumont
Kathleen Clark
Center for High Throughput Structural Biology
PSI
Kathleen Clark
Center for High Throughput Structural Biology
PSI
Publications:
PMID:
20045057
Title: Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ligation independent cloning
LIC
Ligation independent cloning site
0
0
promoter
Ura3 promoter
Ura3 promoter
6360
6389
protease cleavage site
3C
Rhinovirus 3C protease cleavage site
4233
4256
selectable marker
AmpR
Ampicillin resistance gene
1742
2401
selectable marker
Ura3
Ura3 nutritional marker (yeast)
5471
6274
tag
His
10x His tag
4653
4682
