DNASU Plasmid | TM0342 (T. maritima ) in pMH1TEV (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM0342

Gene Name: permease

Original Clone ID:

24461

Keyword:

None

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH1TEV

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00305710

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1242nts

Open reading frame: 51 to 1292

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAATGAGA
TCTTACACTGGAAGAGGAACTTCTTCCTTCTCGCACTCGGAAGATTCGTATCGATTCTTG
GAAGTTCCGTTCAGGCGGTTGCGATTCCTCTTTTTGTACTGGATCTGACACACTCCGGAT
CTGCCGTTGCCCTGATGACCGTAGCCTACATGGTTCCAAGGATCCTTTTCACACCCATAG
CCGGGATCGTGGGAGACAGGGGAGACAGGAAGTTCCTCATGGTGTCGATGGATTTTTTGA
GGGGATTTCTCATCTGTTTTCTGGCTCTTCTCGCGTTCAGGAACTCCATCACTCTTCCCG
TTCTCTACGTTTCTCAGATAGCCATGGCTATTATGGACAATCTCTTCGACATACCGACAG
GCGCGATGTTTCCCGATATTGTTCCGCAGGATTTTCTCATGAGAGCCAACTCCATCATGG
GTATGGTGAGGATCTTTCCTCAGATTCTGGGTCCTGCACTGGGAGGAGTGATCTACGGAT
TTTACGGTATAGCGATCGTGTTTCTTGTGAATGGAGTGTCTTTTGTCCTCTCTGCCATCA
GTGAGATCTTCATCGTTTACAGCCGAAAGGTGGAGAAAAAGCCAGCAAAATTCTCTCAGA
TCCTTGAAGATCTCAAAGAAGGATTTCTCTTCATCTGGAACCACGAGCTCATACGAACCA
TTCTGATCTTCGCACTTTTTCTGAATCTGCTCATCTCTCCGCTGTTCATGGTGGTGTATC
CTTACACGATCAGGGAAGTGATGAAATTTTCGGCTCAGGAATTTGGTTTCCTGGAGACCT
CCTTCACTTTAGGGGCACTCCTTGGGAACTTTTTGATTGCATCGTACCTGTCGAAGAAGA
ACATGTGGAAGGCCATGAAGATCTCTATCTTTCTTTTTGAGCTTCCCATCGTCTTCCTTG
GCTTTCTCGTTATGCCTGATTTTTCCTTGAACAGACTGGTCCTCTTCTCGTTGTTTTTCA
TCACTTTCCTGATAATGGGTTTTTTGAATCCCATCATCAACGTTCCGCTGGACACGGCGT
TTCAAAAGATGACACCTTCTCACATCAGAGCGAGGGCCTTTTCTTTCCTCATTCTCGTGG
CAAACGGAGTCACTCCGCTCGGATCGGTTCTCTACGGATACCTCGTTGATAGAGTGCCGG
TCCATTTTCTCTACTACGTTGTCGGGGCACTTTCTTTTCTGGTCGCTGTACTGCTTCTGG
TAGCTTTGAGGGGAAAAGAGCTTCCCACTTAA

Protein sequence predicted based on nucleotide sequence

MNEILHWKRNFFLLALGRFVSILGSSVQAVAIPLFVLDLTHSGSAVALMTVAYMVPRILF
TPIAGIVGDRGDRKFLMVSMDFLRGFLICFLALLAFRNSITLPVLYVSQIAMAIMDNLFD
IPTGAMFPDIVPQDFLMRANSIMGMVRIFPQILGPALGGVIYGFYGIAIVFLVNGVSFVL
SAISEIFIVYSRKVEKKPAKFSQILEDLKEGFLFIWNHELIRTILIFALFLNLLISPLFM
VVYPYTIREVMKFSAQEFGFLETSFTLGALLGNFLIASYLSKKNMWKAMKISIFLFELPI
VFLGFLVMPDFSLNRLVLFSLFFITFLIMGFLNPIINVPLDTAFQKMTPSHIRARAFSFL
ILVANGVTPLGSVLYGYLVDRVPVHFLYYVVGALSFLVAVLLLVALRGKELPT*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1242

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Protein_Purified

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in 282217

Recommended expression in:

Not Applicable

Vector Name:

pMH1TEV

Synonyms:

pMH-1-TEV

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; amp resistance; PIPE cloning.

Comments:

None

Size (bp):

4069

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTTGGGCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCGCCTTATAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type bacterial origin of replication

1908

2590

inducible trxn activator protein gene

araC

araC cds

3163

4035

promoter

araC

araC promoter

256

285

protease cleavage site

TEV

TEV protease cleavage site

382

402

selectable marker

AmpR

ampicillin resistance gene

1151

1810

tag

His

N-terminal 6xHis tag

364

381

trxn regulatory element

arabinose O1 operator

trxn regulatory element

arabinose O2 operator

161

182

trxn termination sequence

rrnB

rrnB T2 transcriptional terminator

813

839

Gene

Gene Symbol :

TM0342

GENEID :

897298

Locus Tag :

TM0342

GenBank Accession :

AAD35428

Target GenBank:

NP_228153

Protein

Structural Annotation Wiki :

TOPSAN

Swissprot ID :

Q9WYH7

Clone

Cloning Information :

289500

HIP Master Clone ID :

218986

Original Clone ID :

24461

Experimental

TargetTrack - Experimental data :

282217

Organism

Species Specific ID:

897298