DNASU Plasmid | RFC4 (Homo sapiens) in pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: RFC4

Gene Name: replication factor C (activator 1) 4, 37kDa

Original Clone ID:

61020

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pSpeedET

Format:

FUSION

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

HsCD00326067

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1092nts

Open reading frame: 51 to 1142

Reference Sequence Alignment

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGCAAGCAT
TTCTTAAAGGTACATCCATCAGTACTAAACCCCCGCTGACCAAGGATCGAGGAGTAGCTG
CCAGTGCGGGAAGTAGCGGAGAGAACAAGAAAGCCAAACCCGTTCCCTGGGTGGAAAAAT
ATCGCCCAAAATGTGTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCAGTGCTGAAAA
AATCTTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCTGGAACTGGAA
AAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCTGAACTTTTCCGATTAA
GAGTTCTTGAGTTAAATGCATCTGATGAACGTGGAATACAAGTAGTTCGAGAGAAAGTGA
AAAATTTTGCTCAATTAACTGTGTCAGGAAGTCGCTCAGATGGGAAGCCGTGTCCGCCTT
TTAAGATTGTGATTCTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAGGCAGCTTTAA
GACGTACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGTAACTATGTCA
GTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGCTTCAAGCCTCTGTCAG
ATAAAATTCAACAGCAGCGATTACTAGACATTGCCAAGAAGGAAAATGTCAAAATTAGTG
ATGAGGGAATAGCTTATCTTGTTAAAGTGTCAGAAGGAGACTTAAGAAAAGCCATTACAT
TTCTTCAAAGCGCTACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAAGTGATTACAG
ACATTGCTGGGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCCTGTCAGAGTG
GCTCTTTTGACAAACTAGAAGCTGTGGTCAAGGATTTAATAGATGAGGGTCATGCAGCAA
CTCAGCTCGTCAATCAACTCCATGATGTGGTTGTAGAAAATAACTTATCTGATAAACAGA
AGTCTATTATCACAGAAAAACTTGCCGAAGTTGACAAATGCCTAGCAGATGGTGCTGATG
AACATTTGCAACTCATCAGCCTTTGTGCAACTGTGATGCAGCAGTTATCTCAGAATTGTT
AACGCGACTTAATTAA

Protein sequence predicted based on nucleotide sequence

MQAFLKGTSISTKPPLTKDRGVAASAGSSGENKKAKPVPWVEKYRPKCVDEVAFQEEVVA
VLKKSLEGADLPNLLFYGPPGTGKTSTILAAARELFGPELFRLRVLELNASDERGIQVVR
EKVKNFAQLTVSGSRSDGKPCPPFKIVILDEADSMTSAAQAALRRTMEKESKTTRFCLIC
NYVSRIIEPLTSRCSKFRFKPLSDKIQQQRLLDIAKKENVKISDEGIAYLVKVSEGDLRK
AITFLQSATRLTGGKEITEKVITDIAGVIPAEKIDGVFAACQSGSFDKLEAVVKDLIDEG
HAATQLVNQLHDVVVENNLSDKQKSIITEKLAEVDKCLADGADEHLQLISLCATVMQQLS
QNC*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in 400433

Recommended expression in:

Not Applicable

Vector Name:

pSpeedET

Synonyms:

pSpeed-ET

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.

Comments:

If this vector contains an insert, the ccdB death cassette will not be present.

Size (bp):

5464

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

PMID: 18004753

Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCGCCGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAACTTAATTAACTCTAGATAACTGAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type origin of replication

3306

3988

inducible trxn activator protein gene

araC

araC coding sequence

4563

5438

negative selection marker

ccdB

ccdB death cassette gene (lost in with-insert form)

1596

1805

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

protease cleavage site

TEV

TEV protease cleavage site

382

402

selectable marker

CmR

chloramphenicol resistance gene (CmR) (lost in with-insert form)

502

1158

selectable marker

KanR

kanamycin resistance gene

2396

3208

tag

His

N-terminal 6xHis tag

364

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

trxn termination sequence

rrnB

rrnB T2 transcription terminator

2258

2284

Gene

Gene Symbol :

RFC4

Symbol Nomenclature :

RFC4

SYNONYM :

A1; RFC37

Designation :

Full Nomenclature :

replication factor C (activator 1) 4, 37kDa

GENEID :

5984

GI :

31881687

GenBank Accession :

NM_181573

HGNC :

9972

MIM :

102577

Vega :

OTTHUMG00000156515

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00326067      CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGCAAGCAT
NM_181573.2       --------------------------------------------------ATGCAAGCAT
NM_002916.3       --------------------------------------------------ATGCAAGCAT
                                                                    **********

HsCD00326067      TTCTTAAAGGTACATCCATCAGTACTAAACCCCCGCTGACCAAGGATCGAGGAGTAGCTG
NM_181573.2       TTCTTAAAGGTACATCCATCAGTACTAAACCCCCGCTGACCAAGGATCGAGGAGTAGCTG
NM_002916.3       TTCTTAAAGGTACATCCATCAGTACTAAACCCCCGCTGACCAAGGATCGAGGAGTAGCTG
                  ************************************************************

HsCD00326067      CCAGTGCGGGAAGTAGCGGAGAGAACAAGAAAGCCAAACCCGTTCCCTGGGTGGAAAAAT
NM_181573.2       CCAGTGCGGGAAGTAGCGGAGAGAACAAGAAAGCCAAACCCGTTCCCTGGGTGGAAAAAT
NM_002916.3       CCAGTGCGGGAAGTAGCGGAGAGAACAAGAAAGCCAAACCCGTTCCCTGGGTGGAAAAAT
                  ************************************************************

HsCD00326067      ATCGCCCAAAATGTGTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCAGTGCTGAAAA
NM_181573.2       ATCGCCCAAAATGTGTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCAGTGCTGAAAA
NM_002916.3       ATCGCCCAAAATGTGTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCAGTGCTGAAAA
                  ************************************************************

HsCD00326067      AATCTTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCTGGAACTGGAA
NM_181573.2       AATCTTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCTGGAACTGGAA
NM_002916.3       AATCTTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCTGGAACTGGAA
                  ************************************************************

HsCD00326067      AAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCTGAACTTTTCCGATTAA
NM_181573.2       AAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCTGAACTTTTCCGATTAA
NM_002916.3       AAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCTGAACTTTTCCGATTAA
                  ************************************************************

HsCD00326067      GAGTTCTTGAGTTAAATGCATCTGATGAACGTGGAATACAAGTAGTTCGAGAGAAAGTGA
NM_181573.2       GAGTTCTTGAGTTAAATGCATCTGATGAACGTGGAATACAAGTAGTTCGAGAGAAAGTGA
NM_002916.3       GAGTTCTTGAGTTAAATGCATCTGATGAACGTGGAATACAAGTAGTTCGAGAGAAAGTGA
                  ************************************************************

HsCD00326067      AAAATTTTGCTCAATTAACTGTGTCAGGAAGTCGCTCAGATGGGAAGCCGTGTCCGCCTT
NM_181573.2       AAAATTTTGCTCAATTAACTGTGTCAGGAAGTCGCTCAGATGGGAAGCCGTGTCCGCCTT
NM_002916.3       AAAATTTTGCTCAATTAACTGTGTCAGGAAGTCGCTCAGATGGGAAGCCGTGTCCGCCTT
                  ************************************************************

HsCD00326067      TTAAGATTGTGATTCTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAGGCAGCTTTAA
NM_181573.2       TTAAGATTGTGATTCTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAGGCAGCTTTAA
NM_002916.3       TTAAGATTGTGATTCTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAGGCAGCTTTAA
                  ************************************************************

HsCD00326067      GACGTACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGTAACTATGTCA
NM_181573.2       GACGTACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGTAACTATGTCA
NM_002916.3       GACGTACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGTAACTATGTCA
                  ************************************************************

HsCD00326067      GTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGCTTCAAGCCTCTGTCAG
NM_181573.2       GTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGCTTCAAGCCTCTGTCAG
NM_002916.3       GTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGCTTCAAGCCTCTGTCAG
                  ************************************************************

HsCD00326067      ATAAAATTCAACAGCAGCGATTACTAGACATTGCCAAGAAGGAAAATGTCAAAATTAGTG
NM_181573.2       ATAAAATTCAACAGCAGCGATTACTAGACATTGCCAAGAAGGAAAATGTCAAAATTAGTG
NM_002916.3       ATAAAATTCAACAGCAGCGATTACTAGACATTGCCAAGAAGGAAAATGTCAAAATTAGTG
                  ************************************************************

HsCD00326067      ATGAGGGAATAGCTTATCTTGTTAAAGTGTCAGAAGGAGACTTAAGAAAAGCCATTACAT
NM_181573.2       ATGAGGGAATAGCTTATCTTGTTAAAGTGTCAGAAGGAGACTTAAGAAAAGCCATTACAT
NM_002916.3       ATGAGGGAATAGCTTATCTTGTTAAAGTGTCAGAAGGAGACTTAAGAAAAGCCATTACAT
                  ************************************************************

HsCD00326067      TTCTTCAAAGCGCTACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAAGTGATTACAG
NM_181573.2       TTCTTCAAAGCGCTACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAAGTGATTACAG
NM_002916.3       TTCTTCAAAGCGCTACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAAGTGATTACAG
                  ************************************************************

HsCD00326067      ACATTGCTGGGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCCTGTCAGAGTG
NM_181573.2       ACATTGCCGGGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCCTGTCAGAGTG
NM_002916.3       ACATTGCCGGGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCCTGTCAGAGTG
                  ******* ****************************************************

HsCD00326067      GCTCTTTTGACAAACTAGAAGCTGTGGTCAAGGATTTAATAGATGAGGGTCATGCAGCAA
NM_181573.2       GCTCTTTTGACAAACTAGAAGCTGTGGTCAAGGATTTAATAGATGAGGGTCATGCAGCAA
NM_002916.3       GCTCTTTTGACAAACTAGAAGCTGTGGTCAAGGATTTAATAGATGAGGGTCATGCAGCAA
                  ************************************************************

HsCD00326067      CTCAGCTCGTCAATCAACTCCATGATGTGGTTGTAGAAAATAACTTATCTGATAAACAGA
NM_181573.2       CTCAGCTCGTCAATCAACTCCATGATGTGGTTGTAGAAAATAACTTATCTGATAAACAGA
NM_002916.3       CTCAGCTCGTCAATCAACTCCATGATGTGGTTGTAGAAAATAACTTATCTGATAAACAGA
                  ************************************************************

HsCD00326067      AGTCTATTATCACAGAAAAACTTGCCGAAGTTGACAAATGCCTAGCAGATGGTGCTGATG
NM_181573.2       AGTCTATTATCACAGAAAAACTTGCCGAAGTTGACAAATGCCTAGCAGATGGTGCTGATG
NM_002916.3       AGTCTATTATCACAGAAAAACTTGCCGAAGTTGACAAATGCCTAGCAGATGGTGCTGATG
                  ************************************************************

HsCD00326067      AACATTTGCAACTCATCAGCCTTTGTGCAACTGTGATGCAGCAGTTATCTCAGAATTGTT
NM_181573.2       AACATTTGCAACTCATCAGCCTTTGTGCAACTGTGATGCAGCAGTTATCTCAGAATTGTT
NM_002916.3       AACATTTGCAACTCATCAGCCTTTGTGCAACTGTGATGCAGCAGTTATCTCAGAATTGTT
                  ************************************************************

HsCD00326067      AACGCGACTTAATTAA
NM_181573.2       AA--------------
NM_002916.3       AA--------------
                  **

Pathways

NCI :

ATR signaling pathway

NCI :

Fanconi anemia pathway

Panther :

DNA replication

Reactome :

Activation of ATR in response to replication stress

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

Cell Cycle, Mitotic

Reactome :

Chromosome Maintenance

Reactome :

DNA Repair

Reactome :

DNA Replication

Reactome :

DNA strand elongation

Reactome :

Extension of Telomeres

Reactome :

G2/M Checkpoints

Reactome :

Gap-filling DNA repair synthesis and ligation in GG-NER

Reactome :

Gap-filling DNA repair synthesis and ligation in TC-NER

Reactome :

Global Genomic NER (GG-NER)

Reactome :

Lagging Strand Synthesis

Reactome :

Leading Strand Synthesis

Reactome :

Nucleotide Excision Repair

Reactome :

Polymerase switching

Reactome :

Polymerase switching on the C-strand of the telomere

Reactome :

Repair synthesis for gap-filling by DNA polymerase in TC-NER

Reactome :

Repair synthesis of patch ~27-30 bases long by DNA polymerase

Reactome :

S Phase

Reactome :

Synthesis of DNA

Reactome :

Telomere C-strand (Lagging Strand) Synthesis

Reactome :

Telomere Maintenance

Reactome :

Transcription-coupled NER (TC-NER)

WikiPathway :

DNA Replication

Protein

SMART domain :

AAA : ATPases associated with a variety of cellular activities

Structural Annotation Wiki :

TOPSAN

UniProt :

P35249

HPRD :

00022

Clone

Cloning Information :

522616

HIP Master Clone ID :

432936

Original Clone ID :

61020

Experimental

TargetTrack - Experimental data :

400433

Organism

Species Specific ID:

5984

Genome

Chromosome :

Map Location :

3q27

Ensembl :

ENSG00000163918

Reagents

Labome :

RFC4-antibody