Gene:
Gene Symbol:
BRF2
Gene Name:
BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit
Original Clone ID:
FLH268322.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLenti6.2/V5-DEST
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HsCD00330143
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCAGGCAGAGGCCGCTGCCCGGACTGCGGCTCCACGGAGCTGGTGGAAGACTCGCAC
TATTCGCAGAGCCAGCTGGTGTGCTCCGACTGCGGCTGCGTGGTCACCGAGGGGGTCCTT
ACCACTACCTTCAGCGACGAGGGCAATCTCCGAGAGGTAACATATTCCCGAAGCACAGGG
GAAAACGAACAAGTTAGTCGCAGCCAGCAACGAGGTCTCCGGCGAGTGAGAGACCTTTGT
CGAGTTCTGCAGTTGCCACCAACATTTGAGGATACCGCGGTTGCCTACTACCAACAGGCA
TATCGGCACTCTGGCATCCGAGCGGCCAGGCTGCAAAAGAAGGAGGTGTTGGTTGGGTGC
TGCGTCTTAATCACCTGCCGACAGCATAACTGGCCCCTAACAATGGGGGCCATCTGCACG
CTGTTGTATGCAGATTTGGATGTGTTTTCTAGCACTTACATGCAGATAGTGAAGCTCCTG
GGACTGGATGTGCCATCTCTGTGCTTGGCAGAACTGGTGAAGACCTATTGCAGCAGCTTC
AAACTGTTCCAAGCTTCACCTTCTGTGCCAGCCAAATACGTGGAAGACAAAGAGAAGATG
CTGTCTCGAACAATGCAGTTGGTGGAGCTGGCAAATGAGACGTGGCTGGTGACCGGGAGG
CATCCCTTGCCCGTCATCACTGCTGCGACTTTCCTGGCTTGGCAGTCGCTGCAGCCTGCA
GATCGGCTTTCATGTTCCCTTGCCCGATTTTGTAAATTGGCAAATGTGGACCTGCCCTAC
CCGGCGTCCTCCCGCCTGCAGGAGCTGCTGGCTGTGCTGCTGCGGATGGCTGAGCAGCTG
GCCTGGTTACGAGTTCTGAGACTTGACAAACGGTCTGTGGTGAAGCACATCGGTGACCTT
CTCCAGCACCGCCAGTCACTGGTCCGCTCTGCCTTTCGGGATGGGACAGCAGAAGTGGAG
ACCCGAGAGAAGGAGCCACCGGGGTGGGGACAGGGGCAAGGAGAAGGGGAGGTGGGAAAT
AATTCCTTAGGTTTACCCCAGGGGAAGCGGCCGGCCAGTCCTGCCCTTCTCTTGCCACCC
TGCATGTTGAAGTCCCCGAAGCGGATCTGCCCTGTACCCCCTGTCTCCACTGTCACTGGA
GATGAGAACATTTCTGATAGTGAAATAGAACAGTATTTGCGTACCCCTCAGGAAGTTAGG
GACTTTCAGAGAGCCCAGGCTGCTAGACAGGCTGCCACGAGTGTCCCTAACCCTCCCTTG
Protein sequence predicted based on nucleotide sequence
MPGRGRCPDCGSTELVEDSHYSQSQLVCSDCGCVVTEGVLTTTFSDEGNLREVTYSRSTG ENEQVSRSQQRGLRRVRDLCRVLQLPPTFEDTAVAYYQQAYRHSGIRAARLQKKEVLVGC CVLITCRQHNWPLTMGAICTLLYADLDVFSSTYMQIVKLLGLDVPSLCLAELVKTYCSSF KLFQASPSVPAKYVEDKEKMLSRTMQLVELANETWLVTGRHPLPVITAATFLAWQSLQPA DRLSCSLARFCKLANVDLPYPASSRLQELLAVLLRMAEQLAWLRVLRLDKRSVVKHIGDL LQHRQSLVRSAFRDGTAEVETREKEPPGWGQGQGEGEVGNNSLGLPQGKRPASPALLLPP CMLKSPKRICPVPPVSTVTGDENISDSEIEQYLRTPQEVRDFQRAQAARQAATSVPNPPL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence
1343
Start on reference sequence
84
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: mammalian Marker: blasticidin
Host Type: mammalian Marker: blasticidin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLenti6.2/V5-DEST
Synonyms:
pLenti6.2/V5-DEST
Sequencing Primer:
Forward:
CMV forward
Reverse:
V5 reverse
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection, mammalian expression expression with RSV and 5pLTR and HIV-1 psi and RRE and gp41 peptide and CMV enhancer and CMV Promoter and attR1 and lac UV5 promoter and CmR and ccdB and attR2 and V5 and PGK and EM7 and BlastR and 3pLTR and SV40 and SV40 Ori and T7 promoter and M13 forward and f1 ori and AmpR promoter and AmpR and ori and CAP binding site and lac promoter and lac operator and M13 reverse and T3; blasticidin resistance in mammalian, ampicillin resistance in bacterial;
Comments:
None
Size (bp):
8812
Parent Vector:
None
Properties:
Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
3'UTR
5pLTR
truncated 5' long terminal repeat (LTR) from HIV-1
230
410
3pUTR
3pLTR
self-inactivating 3' long terminal repeat (LTR) from HIV-1
5333
5566
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
3674
3979
CDS
BlastR
confers resistance to blasticidin
4848
5246
CDS
CmR
confers resistance to chloramphenicol
2673
3332
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
6727
7587
CDS
V5
epitope tag from simian virus 5
4197
4238
CDS
gp41 peptide
antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)
1493
1537
Miscellaneous Feature
HIV-1 psi
packaging signal of human immunodeficiency virus type 1
457
582
Miscellaneous Feature
RRE
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
1075
1308
enhancer
CMV enhancer
human cytomegalovirus immediate early enhancer
1816
2119
poly-A signal
SV40
SV40 polyadenylation signal
5638
5759
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
5983
5999
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
8732
8748
promoter
CMV Promoter
human cytomegalovirus (CMV) immediate early promoter
2120
2323
promoter
PGK
mouse phosphoglycerate kinase 1 promoter
4271
4768
promoter
RSV
Rous sarcoma virus enhancer/promoter
3
229
promoter
lac UV5 promoter
E. coli lac promoter with an "up" mutation
2589
2619
promoter
EM7
synthetic bacterial promoter
4782
4829
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
5955
5973
promoter
AmpR promoter
null
6622
6726
promoter
lac promoter
promoter for the E. coli lac operon
8670
8700
promoter
T3
promoter for bacteriophage T3 RNA polymerase
8769
8787
protein binding site
attR1
recombination site for the Gateway LR reaction
2440
2564
protein binding site
attR2
recombination site for the Gateway LR reaction
4020
4144
protein binding site
CAP binding site
CAP binding activates transcription in the presence of cAMP.
8634
8655
protein binding site
lac operator
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
8708
8724
rep_origin
SV40 Ori
SV40 origin of replication
5799
5934
rep_origin
f1 ori
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
6141
6596
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
7758
8346
