DNASU Plasmid | ENC1 (H. sapiens )<br>In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:

Gene Symbol: ENC1

Gene Name: ectodermal-neural cortex 1 (with BTB domain)

Original Clone ID:

NCI10266

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pMCSG19

Format:

CLOSED

Source:

Argonne National Laboratory and National Cancer Institute Clinical Proteomic Technologies for Cancer

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Argonne National Laboratory and National Cancer Institute Clinical Proteomic Technologies for Cancer

HsCD00343270

Price:

AVAILABLE

No restriction
Special MTA: SPTA

Insert Sequence
Protein Sequence

Insert sequence: 1770nts

Open reading frame: 1 to 1770

Reference Sequence Alignment

ATGTCAGTCAGTGTGCATGAGAACCGCAAGTCCAGGGCCAGCAGCGGCTCCATTAACATC
TATCTGTTTCACAAGTCCTCCTACGCTGACAGCGTCCTCACTCACCTGAATCTTTTACGC
CAGCAGCGTCTCTTCACTGACGTCCTTCTCCATGCCGGAAATAGGACCTTCCCTTGCCAC
CGGGCAGTGCTGGCTGCATGCAGTCGCTACTTTGAGGCCATGTTCAGTGGTGGCCTGAAA
GAGAGCCAGGACAGTGAGGTCAACTTTGACAATTCCATCCACCCAGAAGTCTTGGAGCTG
CTGCTTGACTATGCGTACTCCTCCCGGGTCATCATCAATGAAGAAAATGCAGAATCGCTC
CTGGAAGCTGGTGACATGCTGGAGTTTCAAGACATCCGGGATGCATGTGCAGAGTTCCTG
GAAAAGAACCTGCATCCCACCAACTGCCTGGGCATGCTGCTGCTGTCTGATGCACACCAG
TGCACCAAGCTGTACGAACTATCTTGGAGAATGTGTCTCAGCAACTTCCAAACCATCAGG
AAGAATGAAGATTTCCTCCAGCTGCCCCAGGACATGGTAGTGCAACTCTTGTCCAGTGAA
GAGCTGGAGACAGAGGATGAAAGGCTTGTGTACGAGTCTGCAATTAACTGGATCAGCTAT
GACCTGAAGAAGCGCTATTGCTACCTCCCAGAACTGTTGCAGACAGTAAGGCTGGCACTT
CTGCCAGCCATCTATCTCATGGAGAATGTGGCCATGGAGGAACTCATCACCAAGCAGAGA
AAGAGTAAGGAAATTGTGGAAGAGGCCATCAGGTGCAAACTGAAAATCCTGCAGAATGAC
GGTGTGGTAACCAGCCTCTGTGCCCGACCTCGGAAAACTGGCCATGCCCTCTTCCTTCTG
GGAGGACAGACTTTCATGTGTGACAAGTTGTATCTGGTAGACCAGAAGGCCAAAGAAATC
ATTCCCAAGGCTGACATTCCCAGCCCAAGAAAAGAGTTTAGTGCATGTGCGATTGGCTGC
AAAGTGTACATTACTGGGGGGCGGGGGTCTGAAAATGGGGTCTCAAAAGATGTCTGGGTT
TATGATACCCTGCACGAGGAGTGGTCCAAGGCTGCCCCCATGCTGGTGGCCAGGTTTGGC
CATGGCTCTGCTGAACTGAAGCACTGCCTGTATGTGGTTGGGGGGCACACGGCCGCAACT
GGCTGCCTCCCGGCCTCCCCCTCAGTCTCTCTAAAGCAGGTAGAACATTATGACCCCACA
ATCAACAAATGGACCATGGTGGCCCCACTCCGAGAAGGCGTTAGCAACGCCGCAGTAGTG
AGTGCCAAACTTAAGTTATTTGCTTTCGGAGGTACCAGTGTCAGTCATGACAAGCTCCCC
AAAGTTCAGTGTTACGATCAGTGTGAAAACAGGTGGACTGTACCGGCCACCTGTCCCCAG
CCCTGGCGTTACACAGCAGCAGCTGTGCTGGGGAACCAGATTTTTATTATGGGGGGTGAT
ACAGAATTCTCTGCCTGCTCTGCTTATAAATTCAACAGTGAGACTTACCAGTGGACCAAG
GTGGGAGATGTGACAGCAAAGCGCATGAGCTGCCATGCTGTGGCCTCTGGAAACAAACTC
TACGTGGTTGGAGGATACTTTGGCATTCAGCGATGCAAGACTTTGGACTGCTACGATCCA
ACATTAGACGTGTGGAACAGCATCACCACTGTCCCGTACTCGCTGATTCCTACTGCATTT
GTCAGCACCTGGAAACATCTGCCTTCTTAA

Protein sequence predicted based on nucleotide sequence

MSVSVHENRKSRASSGSINIYLFHKSSYADSVLTHLNLLRQQRLFTDVLLHAGNRTFPCH
RAVLAACSRYFEAMFSGGLKESQDSEVNFDNSIHPEVLELLLDYAYSSRVIINEENAESL
LEAGDMLEFQDIRDACAEFLEKNLHPTNCLGMLLLSDAHQCTKLYELSWRMCLSNFQTIR
KNEDFLQLPQDMVVQLLSSEELETEDERLVYESAINWISYDLKKRYCYLPELLQTVRLAL
LPAIYLMENVAMEELITKQRKSKEIVEEAIRCKLKILQNDGVVTSLCARPRKTGHALFLL
GGQTFMCDKLYLVDQKAKEIIPKADIPSPRKEFSACAIGCKVYITGGRGSENGVSKDVWV
YDTLHEEWSKAAPMLVARFGHGSAELKHCLYVVGGHTAATGCLPASPSVSLKQVEHYDPT
INKWTMVAPLREGVSNAAVVSAKLKLFAFGGTSVSHDKLPKVQCYDQCENRWTVPATCPQ
PWRYTAAAVLGNQIFIMGGDTEFSACSAYKFNSETYQWTKVGDVTAKRMSCHAVASGNKL
YVVGGYFGIQRCKTLDCYDPTLDVWNSITTVPYSLIPTAFVSTWKHLPS*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Recommended expression in:

Not Applicable

Vector Name:

pMCSG19

Synonyms:

pMCSG-19

Sequencing Primer:

Forward: ANForward

Reverse: T7 terminator

Description:

Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC).

Comments:

pMCSG7 derivative: in vivo TVMV cleavage of MBP

Size (bp):

6441

Parent Vector:

None

Properties:

bacterial expression, improves solubility of insert, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker

Author Name:

Midwest Center for Structural Genomics

Publications:

PMID: 16497515

Title: An expression vector tailored for large-scale, high-throughput purification of recombinant proteins

PMID: 18988021

Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:

Vector Sequence:

Sequence:

ATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCAGTGGTGGTGGTGGTGGTGCTCGAGTGCGGCCGCAAGCTTGTCGACGGAGCTCGAATTCGGATCCGTTATCCACTTCCAATATTGGATTGGAAGTACAGGTTCTCGGTACCCAGATCCACGCCAGAAGAGTGATGATGATGGTGGTGAGACTGGAAACGCACGGTTTCCGAGCCTGCTTTTTTGTACAAACTTGTGATCGAATTAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCCAGACCTTCATCAGTCAGCAGATAGTTTTCGAGGAACTCTTTTGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTCGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCAGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCGGTCGTGTGCCCAGAAGATAATGTCAGGGCCATCGCCAGTTGCCGCAACCTGTGGGAATTTCTCTTCCAGTTTATCCGGATGCTCAACGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTCATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAAATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCA

Vector Features:

Type

Name

Description

Start

End

MCS

LIC

Ligation independent cloning (LIC) site

204

235

bacterial origin

ori

ColE1-type bacterial origin of replication

4416

5098

promoter

T7 promoter

1515

1531

protease cleavage site

TEV

TEV cleavage site cds

223

243

protease cleavage site

TVMV

TVMV protease cleavage site

286

306

repressor binding site

lac operator

LacO fragment

1490

1512

repressor protein gene

LacI

LacI coding sequence

2045

3004

selectable marker

AmpR

ampicillin resistance gene

5193

6053

tag

His

6xHis tag

268

285

tag

His 2

C-terminal 6xHis tag

140

157

tag

MBP

N-terminal MBP

337

1443

trxn termination sequence

T7 term

T7 terminator sequence

Gene

Gene Symbol :

ENC1

Symbol Nomenclature :

ENC1

SYNONYM :

CCL28; ENC-1; KLHL35; KLHL37; NRPB; PIG10; TP53I10

Designation :

Full Nomenclature :

ectodermal-neural cortex 1 (with BTB domain)

GENEID :

8507

GI :

12653299

GenBank Accession :

BC000418

HGNC :

3345

MIM :

605173

Vega :

OTTHUMG00000102059

Target GenBank:

BC000418.2

Reference Sequence Alignment

  

HsCD00343270        ATGTCAGTCAGTGTGCATGAGAACCGCAAGTCCAGGGCCAGCAGCGGCTCCATTAACATC
NM_001256574.1      ATGTCAGTCAGTGTGCATGAGAACCGCAAGTCCAGGGCCAGCAGCGGCTCCATTAACATC
NM_003633.3         ATGTCAGTCAGTGTGCATGAGAACCGCAAGTCCAGGGCCAGCAGCGGCTCCATTAACATC
NM_001256575.1      ATGTCAGTCAGTGTGCATGAGAACCGCAAGTCCAGGGCCAGCAGCGGCTCCATTAACATC
                    ************************************************************

HsCD00343270        TATCTGTTTCACAAGTCCTCCTACGCTGACAGCGTCCTCACTCACCTGAATCTTTTACGC
NM_001256574.1      TATCTGTTTCACAAGTCCTCCTACGCTGACAGCGTCCTCACTCACCTGAATCTTTTACGC
NM_003633.3         TATCTGTTTCACAAGTCCTCCTACGCTGACAGCGTCCTCACTCACCTGAATCTTTTACGC
NM_001256575.1      TATCTGTTTCACAAGTCCTCCTACGCTGACAGCGTCCTCACTCACCTGAATCTTTTACGC
                    ************************************************************

HsCD00343270        CAGCAGCGTCTCTTCACTGACGTCCTTCTCCATGCCGGAAATAGGACCTTCCCTTGCCAC
NM_001256574.1      CAGCAGCGTCTCTTCACTGACGTCCTTCTCCATGCCGGAAATAGGACCTTCCCTTGCCAC
NM_003633.3         CAGCAGCGTCTCTTCACTGACGTCCTTCTCCATGCCGGAAATAGGACCTTCCCTTGCCAC
NM_001256575.1      CAGCAGCGTCTCTTCACTGACGTCCTTCTCCATGCCGGAAATAGGACCTTCCCTTGCCAC
                    ************************************************************

HsCD00343270        CGGGCAGTGCTGGCTGCATGCAGTCGCTACTTTGAGGCCATGTTCAGTGGTGGCCTGAAA
NM_001256574.1      CGGGCAGTGCTGGCTGCATGCAGTCGCTACTTTGAGGCCATGTTCAGTGGTGGCCTGAAA
NM_003633.3         CGGGCAGTGCTGGCTGCATGCAGTCGCTACTTTGAGGCCATGTTCAGTGGTGGCCTGAAA
NM_001256575.1      CGGGCAGTGCTGGCTGCATGCAGTCGCTACTTTGAGGCCATGTTCAGTGGTGGCCTGAAA
                    ************************************************************

HsCD00343270        GAGAGCCAGGACAGTGAGGTCAACTTTGACAATTCCATCCACCCAGAAGTCTTGGAGCTG
NM_001256574.1      GAGAGCCAGGACAGTGAGGTCAACTTTGACAATTCCATCCACCCAGAAGTCTTGGAGCTG
NM_003633.3         GAGAGCCAGGACAGTGAGGTCAACTTTGACAATTCCATCCACCCAGAAGTCTTGGAGCTG
NM_001256575.1      GAGAGCCAGGACAGTGAGGTCAACTTTGACAATTCCATCCACCCAGAAGTCTTGGAGCTG
                    ************************************************************

HsCD00343270        CTGCTTGACTATGCGTACTCCTCCCGGGTCATCATCAATGAAGAAAATGCAGAATCGCTC
NM_001256574.1      CTGCTTGACTATGCGTACTCCTCCCGGGTCATCATCAATGAAGAAAATGCAGAATCGCTC
NM_003633.3         CTGCTTGACTATGCGTACTCCTCCCGGGTCATCATCAATGAAGAAAATGCAGAATCGCTC
NM_001256575.1      CTGCTTGACTATGCGTACTCCTCCCGGGTCATCATCAATGAAGAAAATGCAGAATCGCTC
                    ************************************************************

HsCD00343270        CTGGAAGCTGGTGACATGCTGGAGTTTCAAGACATCCGGGATGCATGTGCAGAGTTCCTG
NM_001256574.1      CTGGAAGCTGGTGACATGCTGGAGTTTCAAGACATCCGGGATGCATGTGCAGAGTTCCTG
NM_003633.3         CTGGAAGCTGGTGACATGCTGGAGTTTCAAGACATCCGGGATGCATGTGCAGAGTTCCTG
NM_001256575.1      CTGGAAGCTGGTGACATGCTGGAGTTTCAAGACATCCGGGATGCATGTGCAGAGTTCCTG
                    ************************************************************

HsCD00343270        GAAAAGAACCTGCATCCCACCAACTGCCTGGGCATGCTGCTGCTGTCTGATGCACACCAG
NM_001256574.1      GAAAAGAACCTGCATCCCACCAACTGCCTGGGCATGCTGCTGCTGTCTGATGCACACCAG
NM_003633.3         GAAAAGAACCTGCATCCCACCAACTGCCTGGGCATGCTGCTGCTGTCTGATGCACACCAG
NM_001256575.1      GAAAAGAACCTGCATCCCACCAACTGCCTGGGCATGCTGCTGCTGTCTGATGCACACCAG
                    ************************************************************

HsCD00343270        TGCACCAAGCTGTACGAACTATCTTGGAGAATGTGTCTCAGCAACTTCCAAACCATCAGG
NM_001256574.1      TGCACCAAGCTGTACGAACTATCTTGGAGAATGTGTCTCAGCAACTTCCAAACCATCAGG
NM_003633.3         TGCACCAAGCTGTACGAACTATCTTGGAGAATGTGTCTCAGCAACTTCCAAACCATCAGG
NM_001256575.1      TGCACCAAGCTGTACGAACTATCTTGGAGAATGTGTCTCAGCAACTTCCAAACCATCAGG
                    ************************************************************

HsCD00343270        AAGAATGAAGATTTCCTCCAGCTGCCCCAGGACATGGTAGTGCAACTCTTGTCCAGTGAA
NM_001256574.1      AAGAATGAAGATTTCCTCCAGCTGCCCCAGGACATGGTAGTGCAACTCTTGTCCAGTGAA
NM_003633.3         AAGAATGAAGATTTCCTCCAGCTGCCCCAGGACATGGTAGTGCAACTCTTGTCCAGTGAA
NM_001256575.1      AAGAATGAAGATTTCCTCCAGCTGCCCCAGGACATGGTAGTGCAACTCTTGTCCAGTGAA
                    ************************************************************

HsCD00343270        GAGCTGGAGACAGAGGATGAAAGGCTTGTGTACGAGTCTGCAATTAACTGGATCAGCTAT
NM_001256574.1      GAGCTGGAGACAGAGGATGAAAGGCTTGTGTACGAGTCTGCAATTAACTGGATCAGCTAT
NM_003633.3         GAGCTGGAGACAGAGGATGAAAGGCTTGTGTACGAGTCTGCAATTAACTGGATCAGCTAT
NM_001256575.1      GAGCTGGAGACAGAGGATGAAAGGCTTGTGTACGAGTCTGCAATTAACTGGATCAGCTAT
                    ************************************************************

HsCD00343270        GACCTGAAGAAGCGCTATTGCTACCTCCCAGAACTGTTGCAGACAGTAAGGCTGGCACTT
NM_001256574.1      GACCTGAAGAAGCGCTATTGCTACCTCCCAGAACTGTTGCAGACAGTAAGGCTGGCACTT
NM_003633.3         GACCTGAAGAAGCGCTATTGCTACCTCCCAGAACTGTTGCAGACAGTAAGGCTGGCACTT
NM_001256575.1      GACCTGAAGAAGCGCTATTGCTACCTCCCAGAACTGTTGCAGACAGTAAGGCTGGCACTT
                    ************************************************************

HsCD00343270        CTGCCAGCCATCTATCTCATGGAGAATGTGGCCATGGAGGAACTCATCACCAAGCAGAGA
NM_001256574.1      CTGCCAGCCATCTATCTCATGGAGAATGTGGCCATGGAGGAACTCATCACCAAGCAGAGA
NM_003633.3         CTGCCAGCCATCTATCTCATGGAGAATGTGGCCATGGAGGAACTCATCACCAAGCAGAGA
NM_001256575.1      CTGCCAGCCATCTATCTCATGGAGAATGTGGCCATGGAGGAACTCATCACCAAGCAGAGA
                    ************************************************************

HsCD00343270        AAGAGTAAGGAAATTGTGGAAGAGGCCATCAGGTGCAAACTGAAAATCCTGCAGAATGAC
NM_001256574.1      AAGAGTAAGGAAATTGTGGAAGAGGCCATCAGGTGCAAACTGAAAATCCTGCAGAATGAC
NM_003633.3         AAGAGTAAGGAAATTGTGGAAGAGGCCATCAGGTGCAAACTGAAAATCCTGCAGAATGAC
NM_001256575.1      AAGAGTAAGGAAATTGTGGAAGAGGCCATCAGGTGCAAACTGAAAATCCTGCAGAATGAC
                    ************************************************************

HsCD00343270        GGTGTGGTAACCAGCCTCTGTGCCCGACCTCGGAAAACTGGCCATGCCCTCTTCCTTCTG
NM_001256574.1      GGTGTGGTAACCAGCCTCTGTGCCCGACCTCGGAAAACTGGCCATGCCCTCTTCCTTCTG
NM_003633.3         GGTGTGGTAACCAGCCTCTGTGCCCGACCTCGGAAAACTGGCCATGCCCTCTTCCTTCTG
NM_001256575.1      GGTGTGGTAACCAGCCTCTGTGCCCGACCTCGGAAAACTGGCCATGCCCTCTTCCTTCTG
                    ************************************************************

HsCD00343270        GGAGGACAGACTTTCATGTGTGACAAGTTGTATCTGGTAGACCAGAAGGCCAAAGAAATC
NM_001256574.1      GGAGGACAGACTTTCATGTGTGACAAGTTGTATCTGGTAGACCAGAAGGCCAAAGAAATC
NM_003633.3         GGAGGACAGACTTTCATGTGTGACAAGTTGTATCTGGTAGACCAGAAGGCCAAAGAAATC
NM_001256575.1      GGAGGACAGACTTTCATGTGTGACAAGTTGTATCTGGTAGACCAGAAGGCCAAAGAAATC
                    ************************************************************

HsCD00343270        ATTCCCAAGGCTGACATTCCCAGCCCAAGAAAAGAGTTTAGTGCATGTGCGATTGGCTGC
NM_001256574.1      ATTCCCAAGGCTGACATTCCCAGCCCAAGAAAAGAGTTTAGTGCATGTGCGATTGGCTGC
NM_003633.3         ATTCCCAAGGCTGACATTCCCAGCCCAAGAAAAGAGTTTAGTGCATGTGCGATTGGCTGC
NM_001256575.1      ATTCCCAAGGCTGACATTCCCAGCCCAAGAAAAGAGTTTAGTGCATGTGCGATTGGCTGC
                    ************************************************************

HsCD00343270        AAAGTGTACATTACTGGGGGGCGGGGGTCTGAAAATGGGGTCTCAAAAGATGTCTGGGTT
NM_001256574.1      AAAGTGTACATTACTGGGGGGCGGGGGTCTGAAAATGGGGTCTCAAAAGATGTCTGGGTT
NM_003633.3         AAAGTGTACATTACTGGGGGGCGGGGGTCTGAAAATGGGGTCTCAAAAGATGTCTGGGTT
NM_001256575.1      AAAGTGTACATTACTGGGGGGCGGGGGTCTGAAAATGGGGTCTCAAAAGATGTCTGGGTT
                    ************************************************************

HsCD00343270        TATGATACCCTGCACGAGGAGTGGTCCAAGGCTGCCCCCATGCTGGTGGCCAGGTTTGGC
NM_001256574.1      TATGATACCCTGCACGAGGAGTGGTCCAAGGCTGCCCCCATGCTGGTGGCCAGGTTTGGC
NM_003633.3         TATGATACCCTGCACGAGGAGTGGTCCAAGGCTGCCCCCATGCTGGTGGCCAGGTTTGGC
NM_001256575.1      TATGATACCCTGCACGAGGAGTGGTCCAAGGCTGCCCCCATGCTGGTGGCCAGGTTTGGC
                    ************************************************************

HsCD00343270        CATGGCTCTGCTGAACTGAAGCACTGCCTGTATGTGGTTGGGGGGCACACGGCCGCAACT
NM_001256574.1      CATGGCTCTGCTGAACTGAAGCACTGCCTGTATGTGGTTGGGGGGCACACGGCCGCAACT
NM_003633.3         CATGGCTCTGCTGAACTGAAGCACTGCCTGTATGTGGTTGGGGGGCACACGGCCGCAACT
NM_001256575.1      CATGGCTCTGCTGAACTGAAGCACTGCCTGTATGTGGTTGGGGGGCACACGGCCGCAACT
                    ************************************************************

HsCD00343270        GGCTGCCTCCCGGCCTCCCCCTCAGTCTCTCTAAAGCAGGTAGAACATTATGACCCCACA
NM_001256574.1      GGCTGCCTCCCGGCCTCCCCCTCAGTCTCTCTAAAGCAGGTAGAACATTATGACCCCACA
NM_003633.3         GGCTGCCTCCCGGCCTCCCCCTCAGTCTCTCTAAAGCAGGTAGAACATTATGACCCCACA
NM_001256575.1      GGCTGCCTCCCGGCCTCCCCCTCAGTCTCTCTAAAGCAGGTAGAACATTATGACCCCACA
                    ************************************************************

HsCD00343270        ATCAACAAATGGACCATGGTGGCCCCACTCCGAGAAGGCGTTAGCAACGCCGCAGTAGTG
NM_001256574.1      ATCAACAAATGGACCATGGTGGCCCCACTCCGAGAAGGCGTTAGCAACGCCGCAGTAGTG
NM_003633.3         ATCAACAAATGGACCATGGTGGCCCCACTCCGAGAAGGCGTTAGCAACGCCGCAGTAGTG
NM_001256575.1      ATCAACAAATGGACCATGGTGGCCCCACTCCGAGAAGGCGTTAGCAACGCCGCAGTAGTG
                    ************************************************************

HsCD00343270        AGTGCCAAACTTAAGTTATTTGCTTTCGGAGGTACCAGTGTCAGTCATGACAAGCTCCCC
NM_001256574.1      AGTGCCAAACTTAAGTTATTTGCTTTCGGAGGTACCAGTGTCAGTCATGACAAGCTCCCC
NM_003633.3         AGTGCCAAACTTAAGTTATTTGCTTTCGGAGGTACCAGTGTCAGTCATGACAAGCTCCCC
NM_001256575.1      AGTGCCAAACTTAAGTTATTTGCTTTCGGAGGTACCAGTGTCAGTCATGACAAGCTCCCC
                    ************************************************************

HsCD00343270        AAAGTTCAGTGTTACGATCAGTGTGAAAACAGGTGGACTGTACCGGCCACCTGTCCCCAG
NM_001256574.1      AAAGTTCAGTGTTACGATCAGTGTGAAAACAGGTGGACTGTACCGGCCACCTGTCCCCAG
NM_003633.3         AAAGTTCAGTGTTACGATCAGTGTGAAAACAGGTGGACTGTACCGGCCACCTGTCCCCAG
NM_001256575.1      AAAGTTCAGTGTTACGATCAGTGTGAAAACAGGTGGACTGTACCGGCCACCTGTCCCCAG
                    ************************************************************

HsCD00343270        CCCTGGCGTTACACAGCAGCAGCTGTGCTGGGGAACCAGATTTTTATTATGGGGGGTGAT
NM_001256574.1      CCCTGGCGTTACACAGCAGCAGCTGTGCTGGGGAACCAGATTTTTATTATGGGGGGTGAT
NM_003633.3         CCCTGGCGTTACACAGCAGCAGCTGTGCTGGGGAACCAGATTTTTATTATGGGGGGTGAT
NM_001256575.1      CCCTGGCGTTACACAGCAGCAGCTGTGCTGGGGAACCAGATTTTTATTATGGGGGGTGAT
                    ************************************************************

HsCD00343270        ACAGAATTCTCTGCCTGCTCTGCTTATAAATTCAACAGTGAGACTTACCAGTGGACCAAG
NM_001256574.1      ACAGAATTCTCTGCCTGCTCTGCTTATAAATTCAACAGTGAGACTTACCAGTGGACCAAG
NM_003633.3         ACAGAATTCTCTGCCTGCTCTGCTTATAAATTCAACAGTGAGACTTACCAGTGGACCAAG
NM_001256575.1      ACAGAATTCTCTGCCTGCTCTGCTTATAAATTCAACAGTGAGACTTACCAGTGGACCAAG
                    ************************************************************

HsCD00343270        GTGGGAGATGTGACAGCAAAGCGCATGAGCTGCCATGCTGTGGCCTCTGGAAACAAACTC
NM_001256574.1      GTGGGAGATGTGACAGCAAAGCGCATGAGCTGCCATGCTGTGGCCTCTGGAAACAAACTC
NM_003633.3         GTGGGAGATGTGACAGCAAAGCGCATGAGCTGCCATGCTGTGGCCTCTGGAAACAAACTC
NM_001256575.1      GTGGGAGATGTGACAGCAAAGCGCATGAGCTGCCATGCTGTGGCCTCTGGAAACAAACTC
                    ************************************************************

HsCD00343270        TACGTGGTTGGAGGATACTTTGGCATTCAGCGATGCAAGACTTTGGACTGCTACGATCCA
NM_001256574.1      TACGTGGTTGGAGGATACTTTGGCATTCAGCGATGCAAGACTTTGGACTGCTACGATCCA
NM_003633.3         TACGTGGTTGGAGGATACTTTGGCATTCAGCGATGCAAGACTTTGGACTGCTACGATCCA
NM_001256575.1      TACGTGGTTGGAGGATACTTTGGCATTCAGCGATGCAAGACTTTGGACTGCTACGATCCA
                    ************************************************************

HsCD00343270        ACATTAGACGTGTGGAACAGCATCACCACTGTCCCGTACTCGCTGATTCCTACTGCATTT
NM_001256574.1      ACATTAGACGTGTGGAACAGCATCACCACTGTCCCGTACTCGCTGATTCCTACTGCATTT
NM_003633.3         ACATTAGACGTGTGGAACAGCATCACCACTGTCCCGTACTCGCTGATTCCTACTGCATTT
NM_001256575.1      ACATTAGACGTGTGGAACAGCATCACCACTGTCCCGTACTCGCTGATTCCTACTGCATTT
                    ************************************************************

HsCD00343270        GTCAGCACCTGGAAACATCTGCCTTCTTAA
NM_001256574.1      GTCAGCACCTGGAAACATCTGCCTTCTTAA
NM_003633.3         GTCAGCACCTGGAAACATCTGCCTTCTTAA
NM_001256575.1      GTCAGCACCTGGAAACATCTGCCTTCTTAA
                    ******************************