Gene:
Gene Symbol:
HPS4
Gene Name:
Hermansky-Pudlak syndrome 4
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100000129)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00350873
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCACCTCTACCTCCACAGAGGCAAAGTCAGCCTCGTGGTGGAATTATTTTTTTCTT
TATGATGGTTCCAAGGTAAAGGAAGAAGGCGATCCAACAAGAGCTGGCATTTGTTACTTT
TATCCTTCCCAGACCCTGCTAGACCAACAGGAGTTGCTTTGTGGACAGATTGCTGGAGTT
GTCCGCTGTGTTTCTGACATTTCTGACTCTCCTCCTACTCTTGTTCGTCTGAGAAAACTG
AAGTTTGCCATAAAAGTTGATGGAGATTACCTTTGGGTGCTGGGCTGTGCTGTGGAGCTC
CCTGATGTCAGCTGCAAGCGGTTTCTGGATCAGCTAGTTGGATTCTTTAATTTTTACAAT
GGACCTGTTTCCCTAGCTTATGAGAACTGTTCTCAGGAAGAACTGAGCACGGAGTGGGAC
ACCTTCATCGAGCAAATTCTGAAAAACACCAGTGATCTGCATAAGATTTTCAATTCCCTC
TGGAACTTGGACCAAACTAAAGTGGAGCCCCTGTTGTTGCTGAAGGCAGCCCGCATTCTG
CAGACCTGCCAGCGCTCGCCTCACATTCTCGCTGGCTGCATCCTCTATAAAGGACTGATT
GTCAGCACCCAACTCCCGCCCTCCCTCACCGCCAAGGTCCTGCTTCACCGAACAGCACCT
CAGGAGCAGAGACTCCCTACGGGAGGGGATGCCCCGCAGGAACATGGAGCGGCATTGCCC
CCGAATGTCCAGATTATCCCTGTTTTTGTGACCAAAGAGGAAGCCATTAGTCTCCACGAG
TTCCCGGTGGAACAGATGACAAGGTCTCTAGCATCTCCAGCAGGACTCCAGGATGGTTCA
GCCCAGCACCATCCAAAGGGTGGGAGCACATCTGCCCTGAAAGAAAACGCCACTGGCCAT
GTGGAATCCATGGCCTGGACCACCCCAGATCCCACATCCCCTGACGAAGCTTGTCCAGAT
GGCAGGAAGGAGAACGGATGCTTGTCTGGCCATGATCTGGAGAGCATCAGGCCCGCAGGA
CTGCACAACTCTGCCAGGGGTGAGGTTCTTGGCCTCAGCTCCTCCCTGGGGAAGGAACTA
GTCTTTCTCCAAGAAGAACTCGACTTGTCTGAAATCCACATTCCAGAGGCTCAGGAAGTG
GAAATGGCCTCAGGTCATTTTGCCTTCCTACATGTGCCTGTTCCAGATGGCAGGGCTCCT
TACTGCAAGGCATCTCTCAGCGCCTCCAGCAGCCTGGAACCCACGCCTCCTGAGGACACA
GCCATCAGCAGCTTGCGCCCTCCCTCTGCTCCTGAGATGCTGACCCAGCATGGAGCCCAA
GAGCAGGTCGAAGACCATCCTGGCCATAGCAGCCAAGCCCCCATTCCCAGAGCAGACCCT
CTCCCCAGAAGGACCCGCAGGCCCTTGTTATTGCCTCGCTTAGATCCAGGACAGAGAGGA
AACAAGCTTCCCACGGGGGAACAAGGCCTGGATGAGGATGTTGATGGGGTCTGTGAAAGC
CACGCAGCCCCTGGTCTGGAATGCAGTTCAGGCTCAGCAAACTGTCAGGGTGCTGGCCCC
TCTGCAGATGGAATCAGCTCCAGGCTGACACCAGCAGAGTCCTGCATGGGGCTCGTGAGG
ATGAATCTCTACACTCACTGCGTCAAAGGGCTGATGCTGTCCCTGCTGGCTGAGGAGCCG
CTGCTGGGAGACAGCGCAGCCATAGAGGAAGTGTACCACAGCAGCCTGGCTTCACTGAAT
GGGCTGGAAGTCCACCTGAAAGAGACGCTGCCCAGGGATGAGGCAGCCTCCACGAGCAGC
ACCTACAACTTCACATATTACGACCGCATTCAGAGCTTGCTGATGGCAAACCTGCCGCAG
GTGGCCACCCCGCATGATCGCCGCTTCCTCCAGGCCGTCAGCCTGATGCATAGCGAATTT
GCCCAGCTGCCCGCGCTTTATGAAATGACTGTCAGAAATGCCTCCACGGCTGTGTACGCC
TGTTGCAACCCCATCCAGGAGACATATTTCCAGCAGCTGGCACCTGCAGCACGGAGCTCC
GGCTTCCCAAACCCTCAGGATGGCGCCTTCAGCCTCTCCGGCAAAGCAAAGCAGAAGCTG
CTGAAGCACGGGGTGAACTTGCTCTGA
Protein sequence predicted based on nucleotide sequence
MATSTSTEAKSASWWNYFFLYDGSKVKEEGDPTRAGICYFYPSQTLLDQQELLCGQIAGV VRCVSDISDSPPTLVRLRKLKFAIKVDGDYLWVLGCAVELPDVSCKRFLDQLVGFFNFYN GPVSLAYENCSQEELSTEWDTFIEQILKNTSDLHKIFNSLWNLDQTKVEPLLLLKAARIL QTCQRSPHILAGCILYKGLIVSTQLPPSLTAKVLLHRTAPQEQRLPTGGDAPQEHGAALP PNVQIIPVFVTKEEAISLHEFPVEQMTRSLASPAGLQDGSAQHHPKGGSTSALKENATGH VESMAWTTPDPTSPDEACPDGRKENGCLSGHDLESIRPAGLHNSARGEVLGLSSSLGKEL VFLQEELDLSEIHIPEAQEVEMASGHFAFLHVPVPDGRAPYCKASLSASSSLEPTPPEDT AISSLRPPSAPEMLTQHGAQEQVEDHPGHSSQAPIPRADPLPRRTRRPLLLPRLDPGQRG NKLPTGEQGLDEDVDGVCESHAAPGLECSSGSANCQGAGPSADGISSRLTPAESCMGLVR MNLYTHCVKGLMLSLLAEEPLLGDSAAIEEVYHSSLASLNGLEVHLKETLPRDEAASTSS TYNFTYYDRIQSLLMANLPQVATPHDRRFLQAVSLMHSEFAQLPALYEMTVRNASTAVYA CCNPIQETYFQQLAPAARSSGFPNPQDGAFSLSGKAKQKLLKHGVNLL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
