DNASU Plasmid | ZNF468 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF468

Gene Name: zinc finger protein 468

Original Clone ID:

None

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

The ORFeome Collaboration entry clone (IMAGE:100015547)

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00351383

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1407nts

Open reading frame: 1 to 1407

Reference Sequence Alignment

ATGTTGAAGACGTTGTCGTCAACAGGGCAAGGCAATACAGAAGTGATCCACACAGGGACA
TTGCACAGACAAGCAAGTCATCACATTGGAGAATTTTGTTTCCATGAAATTGAGAAAGAC
ATTCATGGCTTCGAGTTTCAGTGGAAAGAAGATGAAACAAATGGCCATGCAGCACCCATG
ACAGAAATCAAAGAGTTGGCTGGTAGTACAGGCCAACATGATCAAAGGCATGCTGGAAAC
AAGCGTATTAAAGATCAGCTTGGATCAAGCTTTCATTTGCATCTGCCTGAACCGCACATA
TTTCAGTCTGAAGGGAAAATTGGTAATCAAGTTGAGAAGTCTATCAACAATGCTTCCTCA
GTTTCAACATCCCAAAGAATTTGTTGTAGGCCCAAAACCCATATTTCTAATAAGTATGGA
AATAATTCCCTCCATTCTTCATTACTCACACAAAAATGGGAAGTACACATGAGAGAAAAA
TCTTTTGAATGTATACAGAGCTTCAAATCCTTTAATTGCAGCTCACTCTTAAAAAAACAT
CAGATAATTCACTTAGAAGAGAAACAATGTAAATGTGATGTATGTGGCAAGGTCTTTAAT
CAGAAGCGATACCTTGCCTGCCATCGTAGATGTCACACTGGTGAGAAACCTTACAAGTGT
AATGAGTGTGGCAAGACCTTTGGTCATAATTCATCCCTCTTCATTCACAAAGCGCTTCAT
ACTGGAGAGAAACCTTATGAATGTGAAGAATGTGACAAAGTTTTCAGTCGCAAATCACAC
CTTGAAAGACATAAGAGGATTCATACTGGAGAGAAACCATACAAATGTAAGGTTTGTGAT
GAGGCTTTCGCATATAATTCATATCTGGCAAAACATACTATACTTCACACTGGAGAGAAA
CCTTACACATGTAATGAATGTGGCAAAGTTTTTAATCGACTATCAACCCTTGCACGCCAT
CATAGACTTCATACTGGAGAGAAACCTTACAAATGTGAAGAGTGTGACAAAGTTTTCAGT
CGCAAATCACACCTTGAAAGACATAGGAGGATTCATAGTGGAGAGAAACCTTACAAATGT
GAAGAATGTTGCAAAGTTTTCAGTCGCAAATCAAACCTGGAAAGACACAGGAGGATTCAT
ACTGGAGAGAAACCATACAAATGTAAGGTTTGTGACAAGGCTTTCCAGAGGGATTCACAC
CTGGCACAACATCAGAGAGTTCATACTGGAGAGAAACCTTACAAGTGTAATGAGTGTGGC
AAGACCTTCGGTCAGACATCATCGCTTATAATCCATCGTAGGCTTCATACTGGAGAGAAA
CCTTACAAGTGTAATGAGTGTGGCAAGACCTTCAGTCAGATGTCATCCCTTGTATACCAT
CATAGGCTTCATAGTGGAGAGAAGCCT

Protein sequence predicted based on nucleotide sequence

MLKTLSSTGQGNTEVIHTGTLHRQASHHIGEFCFHEIEKDIHGFEFQWKEDETNGHAAPM
TEIKELAGSTGQHDQRHAGNKRIKDQLGSSFHLHLPEPHIFQSEGKIGNQVEKSINNASS
VSTSQRICCRPKTHISNKYGNNSLHSSLLTQKWEVHMREKSFECIQSFKSFNCSSLLKKH
QIIHLEEKQCKCDVCGKVFNQKRYLACHRRCHTGEKPYKCNECGKTFGHNSSLFIHKALH
TGEKPYECEECDKVFSRKSHLERHKRIHTGEKPYKCKVCDEAFAYNSYLAKHTILHTGEK
PYTCNECGKVFNRLSTLARHHRLHTGEKPYKCEECDKVFSRKSHLERHRRIHSGEKPYKC
EECCKVFSRKSNLERHRRIHTGEKPYKCKVCDKAFQRDSHLAQHQRVHTGEKPYKCNECG
KTFGQTSSLIIHRRLHTGEKPYKCNECGKTFSQMSSLVYHHRLHSGEKP

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

ZNF468

Symbol Nomenclature :

ZNF468

Designation :

zinc finger protein ZNF468

Full Nomenclature :

zinc finger protein 468

GENEID :

90333

GenBank Accession :

563990

HGNC :

33105

Vega :

OTTHUMG00000182687

Target GenBank:

BC148554

Reference Sequence Alignment

  

HsCD00351383        ------------------------------------------------------------
NM_001008801.1      ATGGCTCTTCCTCAGGGTCTATTGACATTCAGGGACGTGGCCATAGAATTCTCTCAGGAG
                                                                                

HsCD00351383        ------------------------------------------------------------
NM_001008801.1      GAGTGGAAATGCCTGGACCCTGCTCAGAGGACTTTATACAGGGACGTGATGCTGGAGAAT
                                                                                

HsCD00351383        ---------------------------------------ATGTTGAAGACGTTGTCGTCA
NM_001008801.1      TATAGGAACCTCGTCTCCCTGGATATCTCTTCCAAATGCATGTTGAAGACGTTGTCGTCA
                                                           *********************

HsCD00351383        ACAGGGCAAGGCAATACAGAAGTGATCCACACAGGGACATTGCACAGACAAGCAAGTCAT
NM_001008801.1      ACAGGGCAAGGCAATACAGAAGTGATCCACACAGGGACATTGCACAGACAAGCAAGTCAT
                    ************************************************************

HsCD00351383        CACATTGGAGAATTTTGTTTCCATGAAATTGAGAAAGACATTCATGGCTTCGAGTTTCAG
NM_001008801.1      CACATTGGAGAATTTTGTTTCCATGAAATTGAGAAAGACATTCATGGCTTCGAGTTTCAG
                    ************************************************************

HsCD00351383        TGGAAAGAAGATGAAACAAATGGCCATGCAGCACCCATGACAGAAATCAAAGAGTTGGCT
NM_001008801.1      TGGAAAGAAGATGAAACAAATGGCCATGCAGCACCCATGACAGAAATCAAAGAGTTGGCT
                    ************************************************************

HsCD00351383        GGTAGTACAGGCCAACATGATCAAAGGCATGCTGGAAACAAGCGTATTAAAGATCAGCTT
NM_001008801.1      GGTAGTACAGGCCAACATGATCAAAGGCATGCTGGAAACAAGCGTATTAAAGATCAGCTT
                    ************************************************************

HsCD00351383        GGATCAAGCTTTCATTTGCATCTGCCTGAACCGCACATATTTCAGTCTGAAGGGAAAATT
NM_001008801.1      GGATCAAGCTTTCATTTGCATCTGCCTGAACCGCACATATTTCAGTCTGAAGGGAAAATT
                    ************************************************************

HsCD00351383        GGTAATCAAGTTGAGAAGTCTATCAACAATGCTTCCTCAGTTTCAACATCCCAAAGAATT
NM_001008801.1      GGTAATCAAGTTGAGAAGTCTATCAACAATGCTTCCTCAGTTTCAACATCCCAAAGAATT
                    ************************************************************

HsCD00351383        TGTTGTAGGCCCAAAACCCATATTTCTAATAAGTATGGAAATAATTCCCTCCATTCTTCA
NM_001008801.1      TGTTGTAGGCCCAAAACCCATATTTCTAATAAGTATGGAAATAATTCCCTCCATTCTTCA
                    ************************************************************

HsCD00351383        TTACTCACACAAAAATGGGAAGTACACATGAGAGAAAAATCTTTTGAATGTATACAGAGC
NM_001008801.1      TTACTCACACAAAAATGGGAAGTACACATGAGAGAAAAATCTTTTGAATGTATACAGAGC
                    ************************************************************

HsCD00351383        TTCAAATCCTTTAATTGCAGCTCACTCTTAAAAAAACATCAGATAATTCACTTAGAAGAG
NM_001008801.1      TTCAAATCCTTTAATTGCAGCTCACTCTTAAAAAAACATCAGATAATTCACTTAGAAGAG
                    ************************************************************

HsCD00351383        AAACAATGTAAATGTGATGTATGTGGCAAGGTCTTTAATCAGAAGCGATACCTTGCCTGC
NM_001008801.1      AAACAATGTAAATGTGATGTATGTGGCAAGGTCTTTAATCAGAAGCGATACCTTGCCTGC
                    ************************************************************

HsCD00351383        CATCGTAGATGTCACACTGGTGAGAAACCTTACAAGTGTAATGAGTGTGGCAAGACCTTT
NM_001008801.1      CATCGTAGATGTCACACTGGTGAGAAACCTTACAAGTGTAATGAGTGTGGCAAGACCTTT
                    ************************************************************

HsCD00351383        GGTCATAATTCATCCCTCTTCATTCACAAAGCGCTTCATACTGGAGAGAAACCTTATGAA
NM_001008801.1      GGTCATAATTCATCCCTCTTCATTCACAAAGCGCTTCATACTGGAGAGAAACCTTATGAA
                    ************************************************************

HsCD00351383        TGTGAAGAATGTGACAAAGTTTTCAGTCGCAAATCACACCTTGAAAGACATAAGAGGATT
NM_001008801.1      TGTGAAGAATGTGACAAAGTTTTCAGTCGCAAATCACACCTTGAAAGACATAAGAGGATT
                    ************************************************************

HsCD00351383        CATACTGGAGAGAAACCATACAAATGTAAGGTTTGTGATGAGGCTTTCGCATATAATTCA
NM_001008801.1      CATACTGGAGAGAAACCATACAAATGTAAGGTTTGTGATGAGGCTTTCGCATATAATTCA
                    ************************************************************

HsCD00351383        TATCTGGCAAAACATACTATACTTCACACTGGAGAGAAACCTTACACATGTAATGAATGT
NM_001008801.1      TATCTGGCAAAACATACTATACTTCACACTGGAGAGAAACCTTACACATGTAATGAATGT
                    ************************************************************

HsCD00351383        GGCAAAGTTTTTAATCGACTATCAACCCTTGCACGCCATCATAGACTTCATACTGGAGAG
NM_001008801.1      GGCAAAGTTTTTAATCGACTATCAACCCTTGCACGCCATCATAGACTTCATACTGGAGAG
                    ************************************************************

HsCD00351383        AAACCTTACAAATGTGAAGAGTGTGACAAAGTTTTCAGTCGCAAATCACACCTTGAAAGA
NM_001008801.1      AAACCTTACAAATGTGAAGAGTGTGACAAAGTTTTCAGTCGCAAATCACACCTTGAAAGA
                    ************************************************************

HsCD00351383        CATAGGAGGATTCATAGTGGAGAGAAACCTTACAAATGTGAAGAATGTTGCAAAGTTTTC
NM_001008801.1      CATAGGAGGATTCATAGTGGAGAGAAACCTTACAAATGTGAAGAATGTTGCAAAGTTTTC
                    ************************************************************

HsCD00351383        AGTCGCAAATCAAACCTGGAAAGACACAGGAGGATTCATACTGGAGAGAAACCATACAAA
NM_001008801.1      AGTCGCAAATCAAACCTGGAAAGACACAGGAGGATTCATACTGGAGAGAAACCATACAAA
                    ************************************************************

HsCD00351383        TGTAAGGTTTGTGACAAGGCTTTCCAGAGGGATTCACACCTGGCACAACATCAGAGAGTT
NM_001008801.1      TGTAAGGTTTGTGACAAGGCTTTCCAGAGGGATTCACACCTGGCACAACATCAGAGAGTT
                    ************************************************************

HsCD00351383        CATACTGGAGAGAAACCTTACAAGTGTAATGAGTGTGGCAAGACCTTCGGTCAGACATCA
NM_001008801.1      CATACTGGAGAGAAACCTTACAAGTGTAATGAGTGTGGCAAGACCTTCGGTCAGACATCA
                    ************************************************************

HsCD00351383        TCGCTTATAATCCATCGTAGGCTTCATACTGGAGAGAAACCTTACAAGTGTAATGAGTGT
NM_001008801.1      TCGCTTATAATCCATCGTAGGCTTCATACTGGAGAGAAACCTTACAAGTGTAATGAGTGT
                    ************************************************************

HsCD00351383        GGCAAGACCTTCAGTCAGATGTCATCCCTTGTATACCATCATAGGCTTCATAGTGGAGAG
NM_001008801.1      GGCAAGACCTTCAGTCAGATGTCATCCCTTGTATACCATCATAGGCTTCATAGTGGAGAG
                    ************************************************************

HsCD00351383        AAGCCT---
NM_001008801.1      AAGCCTTAA
                    ******