DNASU Plasmid | KLF10 (Homo sapiens) in pENTR221 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: KLF10

Gene Name: Kruppel-like factor 10

Original Clone ID:

None

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR221

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

The ORFeome Collaboration entry clone (IMAGE:100067205)

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00351621

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1407nts

Open reading frame: 1 to 1407

Reference Sequence Alignment

ATGGAGGAAAGAATGGAAATGATTTCTGAAAGGCCAAAAGAGAGTATGTATTCCTGGAAC
AAAACTGCAGAGAAAAGTGATTTTGAAGCTGTAGAAGCACTTATGTCAATGAGCTGCAGT
TGGAAGTCTGATTTTAAGAAATACGTTGAAAACAGACCTGTTACACCAGTATCTGATTTG
TCAGAGGAAGAGAATCTGCTTCCGGGAACACCTGATTTTCATACAATCCCAGCATTTTGT
TTGACTCCACCTTACAGTCCTTCTGACTTTGAACCCTCTCAAGTGTCAAATCTGATGGCA
CCAGCGCCATCTACTGTACACTTCAAGTCACTCTCAGATACTGCCAAACCTCACATTGCC
GCACCTTTCAAAGAGGAAGAAAAGAGCCCAGTATCTGCCCCCAAACTCCCCAAAGCTCAG
GCAACAAGTGTGATTCGTCATACAGCTGATGCCCAGCTATGTAACCACCAGACCTGCCCA
ATGAAAGCAGCCAGCATCCTCAACTATCAGAACAATTCTTTTAGAAGAAGAACCCACCTA
AATGTTGAGGCTGCAAGAAAGAACATACCATGTGCCGCTGTGTCACCAAACAGATCCAAA
TGTGAGAGAAACACAGTGGCAGATGTTGATGAGAAAGCAAGTGCTGCACTTTATGACTTT
TCTGTGCCTTCCTCAGAGACGGTCATCTGCAGGTCTCAGCCAGCCCCTGTGTCCCCACAA
CAGAAGTCAGTGTTGGTCTCTCCACCTGCAGTATCTGCAGGGGGAGTGCCACCTATGCCG
GTCATCTGCCAGATGGTTCCCCTTCCTGCCAACAACCCTGTTGTGACAACAGTCGTTCCC
AGCACTCCTCCCAGCCAGCCACCAGCCGTTTGCCCCCCTGTTGTGTTCATGGGCACACAA
GTCCCCAAAGGCGCTGTCATGTTTGTGGTACCCCAGCCCGTTGTGCAGAGTTCAAAGCCT
CCGGTGGTGAGCCCGAATGGCACCAGACTCTCTCCCATTGCCCCTGCTCCTGGGTTTTCC
CCTTCAGCAGCAAAAGTCACTCCTCAGATTGATTCATCAAGGATAAGGAGTCACATCTGT
AGCCACCCAGGATGTGGCAAGACATACTTTAAAAGTTCCCATCTGAAGGCCCACACGAGG
ACGCACACAGGAGAAAAGCCTTTCAGCTGTAGCTGGAAAGGTTGTGAAAGGAGGTTTGCC
CGTTCTGATGAACTGTCCAGACACAGGCGAACCCACACGGGTGAGAAGAAATTTGCGTGC
CCCATGTGTGACCGGCGGTTCATGAGGAGTGACCATTTGACCAAGCATGCCCGGCGCCAT
CTATCAGCCAAGAAGCTACCAAACTGGCAGATGGAAGTGAGCAAGCTAAATGACATTGCT
CTACCTCCAACCCCTGCTCCCACACAG

Protein sequence predicted based on nucleotide sequence

MEERMEMISERPKESMYSWNKTAEKSDFEAVEALMSMSCSWKSDFKKYVENRPVTPVSDL
SEEENLLPGTPDFHTIPAFCLTPPYSPSDFEPSQVSNLMAPAPSTVHFKSLSDTAKPHIA
APFKEEEKSPVSAPKLPKAQATSVIRHTADAQLCNHQTCPMKAASILNYQNNSFRRRTHL
NVEAARKNIPCAAVSPNRSKCERNTVADVDEKASAALYDFSVPSSETVICRSQPAPVSPQ
QKSVLVSPPAVSAGGVPPMPVICQMVPLPANNPVVTTVVPSTPPSQPPAVCPPVVFMGTQ
VPKGAVMFVVPQPVVQSSKPPVVSPNGTRLSPIAPAPGFSPSAAKVTPQIDSSRIRSHIC
SHPGCGKTYFKSSHLKAHTRTHTGEKPFSCSWKGCERRFARSDELSRHRRTHTGEKKFAC
PMCDRRFMRSDHLTKHARRHLSAKKLPNWQMEVSKLNDIALPPTPAPTQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR221

Synonyms:

pDONR221, pENTR221-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php.

Size (bp):

2574

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

pUC origin of replication

1905

primer site

M13 F

M13 forward priming site

846

865

primer site

M13 Rev

M13 reverse primer site

846

865

recombination site

attL1

attL1 recombination site

575

675

recombination site

attL2

attL2 recombination site

805

706

recombination site

rrnBT1

rrnB T1 transcription termination sequence

434

477

recombination site

rrnBT2

rrnB T2 transcription termination sequence

275

302

selectable marker

KanR

kanamycin resistance gene

975

1784

Gene

Gene Symbol :

KLF10

Symbol Nomenclature :

KLF10

SYNONYM :

EGR-alpha; EGRA; TIEG; TIEG1

Designation :

Krueppel-like factor 10|TGFB-inducible early growth response protein 1|early growth response-alpha|transforming growth factor-beta-inducible early growth response protein 1|zinc finger transcription factor TIEG

Full Nomenclature :

Kruppel-like factor 10

GENEID :

7071

GenBank Accession :

564228

HGNC :

11810

MIM :

601878

Vega :

OTTHUMG00000164735

Target GenBank:

EU832176

Reference Sequence Alignment

  

HsCD00351621        ---------------------------------ATGGAGGAAAGAATGGAAATGATTTCT
NM_005655.3         ATGCTCAACTTCGGTGCCTCTCTCCAGCAGACTGCGGAGGAAAGAATGGAAATGATTTCT
NM_001032282.3      ---------------------------------ATGGAGGAAAGAATGGAAATGATTTCT
                                                     . *************************

HsCD00351621        GAAAGGCCAAAAGAGAGTATGTATTCCTGGAACAAAACTGCAGAGAAAAGTGATTTTGAA
NM_005655.3         GAAAGGCCAAAAGAGAGTATGTATTCCTGGAACAAAACTGCAGAGAAAAGTGATTTTGAA
NM_001032282.3      GAAAGGCCAAAAGAGAGTATGTATTCCTGGAACAAAACTGCAGAGAAAAGTGATTTTGAA
                    ************************************************************

HsCD00351621        GCTGTAGAAGCACTTATGTCAATGAGCTGCAGTTGGAAGTCTGATTTTAAGAAATACGTT
NM_005655.3         GCTGTAGAAGCACTTATGTCAATGAGCTGCAGTTGGAAGTCTGATTTTAAGAAATACGTT
NM_001032282.3      GCTGTAGAAGCACTTATGTCAATGAGCTGCAGTTGGAAGTCTGATTTTAAGAAATACGTT
                    ************************************************************

HsCD00351621        GAAAACAGACCTGTTACACCAGTATCTGATTTGTCAGAGGAAGAGAATCTGCTTCCGGGA
NM_005655.3         GAAAACAGACCTGTTACACCAGTATCTGATTTGTCAGAGGAAGAGAATCTGCTTCCGGGA
NM_001032282.3      GAAAACAGACCTGTTACACCAGTATCTGATTTGTCAGAGGAAGAGAATCTGCTTCCGGGA
                    ************************************************************

HsCD00351621        ACACCTGATTTTCATACAATCCCAGCATTTTGTTTGACTCCACCTTACAGTCCTTCTGAC
NM_005655.3         ACACCTGATTTTCATACAATCCCAGCATTTTGTTTGACTCCACCTTACAGTCCTTCTGAC
NM_001032282.3      ACACCTGATTTTCATACAATCCCAGCATTTTGTTTGACTCCACCTTACAGTCCTTCTGAC
                    ************************************************************

HsCD00351621        TTTGAACCCTCTCAAGTGTCAAATCTGATGGCACCAGCGCCATCTACTGTACACTTCAAG
NM_005655.3         TTTGAACCCTCTCAAGTGTCAAATCTGATGGCACCAGCGCCATCTACTGTACACTTCAAG
NM_001032282.3      TTTGAACCCTCTCAAGTGTCAAATCTGATGGCACCAGCGCCATCTACTGTACACTTCAAG
                    ************************************************************

HsCD00351621        TCACTCTCAGATACTGCCAAACCTCACATTGCCGCACCTTTCAAAGAGGAAGAAAAGAGC
NM_005655.3         TCACTCTCAGATACTGCCAAACCTCACATTGCCGCACCTTTCAAAGAGGAAGAAAAGAGC
NM_001032282.3      TCACTCTCAGATACTGCCAAACCTCACATTGCCGCACCTTTCAAAGAGGAAGAAAAGAGC
                    ************************************************************

HsCD00351621        CCAGTATCTGCCCCCAAACTCCCCAAAGCTCAGGCAACAAGTGTGATTCGTCATACAGCT
NM_005655.3         CCAGTATCTGCCCCCAAACTCCCCAAAGCTCAGGCAACAAGTGTGATTCGTCATACAGCT
NM_001032282.3      CCAGTATCTGCCCCCAAACTCCCCAAAGCTCAGGCAACAAGTGTGATTCGTCATACAGCT
                    ************************************************************

HsCD00351621        GATGCCCAGCTATGTAACCACCAGACCTGCCCAATGAAAGCAGCCAGCATCCTCAACTAT
NM_005655.3         GATGCCCAGCTATGTAACCACCAGACCTGCCCAATGAAAGCAGCCAGCATCCTCAACTAT
NM_001032282.3      GATGCCCAGCTATGTAACCACCAGACCTGCCCAATGAAAGCAGCCAGCATCCTCAACTAT
                    ************************************************************

HsCD00351621        CAGAACAATTCTTTTAGAAGAAGAACCCACCTAAATGTTGAGGCTGCAAGAAAGAACATA
NM_005655.3         CAGAACAATTCTTTTAGAAGAAGAACCCACCTAAATGTTGAGGCTGCAAGAAAGAACATA
NM_001032282.3      CAGAACAATTCTTTTAGAAGAAGAACCCACCTAAATGTTGAGGCTGCAAGAAAGAACATA
                    ************************************************************

HsCD00351621        CCATGTGCCGCTGTGTCACCAAACAGATCCAAATGTGAGAGAAACACAGTGGCAGATGTT
NM_005655.3         CCATGTGCCGCTGTGTCACCAAACAGATCCAAATGTGAGAGAAACACAGTGGCAGATGTT
NM_001032282.3      CCATGTGCCGCTGTGTCACCAAACAGATCCAAATGTGAGAGAAACACAGTGGCAGATGTT
                    ************************************************************

HsCD00351621        GATGAGAAAGCAAGTGCTGCACTTTATGACTTTTCTGTGCCTTCCTCAGAGACGGTCATC
NM_005655.3         GATGAGAAAGCAAGTGCTGCACTTTATGACTTTTCTGTGCCTTCCTCAGAGACGGTCATC
NM_001032282.3      GATGAGAAAGCAAGTGCTGCACTTTATGACTTTTCTGTGCCTTCCTCAGAGACGGTCATC
                    ************************************************************

HsCD00351621        TGCAGGTCTCAGCCAGCCCCTGTGTCCCCACAACAGAAGTCAGTGTTGGTCTCTCCACCT
NM_005655.3         TGCAGGTCTCAGCCAGCCCCTGTGTCCCCACAACAGAAGTCAGTGTTGGTCTCTCCACCT
NM_001032282.3      TGCAGGTCTCAGCCAGCCCCTGTGTCCCCACAACAGAAGTCAGTGTTGGTCTCTCCACCT
                    ************************************************************

HsCD00351621        GCAGTATCTGCAGGGGGAGTGCCACCTATGCCGGTCATCTGCCAGATGGTTCCCCTTCCT
NM_005655.3         GCAGTATCTGCAGGGGGAGTGCCACCTATGCCGGTCATCTGCCAGATGGTTCCCCTTCCT
NM_001032282.3      GCAGTATCTGCAGGGGGAGTGCCACCTATGCCGGTCATCTGCCAGATGGTTCCCCTTCCT
                    ************************************************************

HsCD00351621        GCCAACAACCCTGTTGTGACAACAGTCGTTCCCAGCACTCCTCCCAGCCAGCCACCAGCC
NM_005655.3         GCCAACAACCCTGTTGTGACAACAGTCGTTCCCAGCACTCCTCCCAGCCAGCCACCAGCC
NM_001032282.3      GCCAACAACCCTGTTGTGACAACAGTCGTTCCCAGCACTCCTCCCAGCCAGCCACCAGCC
                    ************************************************************

HsCD00351621        GTTTGCCCCCCTGTTGTGTTCATGGGCACACAAGTCCCCAAAGGCGCTGTCATGTTTGTG
NM_005655.3         GTTTGCCCCCCTGTTGTGTTCATGGGCACACAAGTCCCCAAAGGCGCTGTCATGTTTGTG
NM_001032282.3      GTTTGCCCCCCTGTTGTGTTCATGGGCACACAAGTCCCCAAAGGCGCTGTCATGTTTGTG
                    ************************************************************

HsCD00351621        GTACCCCAGCCCGTTGTGCAGAGTTCAAAGCCTCCGGTGGTGAGCCCGAATGGCACCAGA
NM_005655.3         GTACCCCAGCCCGTTGTGCAGAGTTCAAAGCCTCCGGTGGTGAGCCCGAATGGCACCAGA
NM_001032282.3      GTACCCCAGCCCGTTGTGCAGAGTTCAAAGCCTCCGGTGGTGAGCCCGAATGGCACCAGA
                    ************************************************************

HsCD00351621        CTCTCTCCCATTGCCCCTGCTCCTGGGTTTTCCCCTTCAGCAGCAAAAGTCACTCCTCAG
NM_005655.3         CTCTCTCCCATTGCCCCTGCTCCTGGGTTTTCCCCTTCAGCAGCAAAAGTCACTCCTCAG
NM_001032282.3      CTCTCTCCCATTGCCCCTGCTCCTGGGTTTTCCCCTTCAGCAGCAAAAGTCACTCCTCAG
                    ************************************************************

HsCD00351621        ATTGATTCATCAAGGATAAGGAGTCACATCTGTAGCCACCCAGGATGTGGCAAGACATAC
NM_005655.3         ATTGATTCATCAAGGATAAGGAGTCACATCTGTAGCCACCCAGGATGTGGCAAGACATAC
NM_001032282.3      ATTGATTCATCAAGGATAAGGAGTCACATCTGTAGCCACCCAGGATGTGGCAAGACATAC
                    ************************************************************

HsCD00351621        TTTAAAAGTTCCCATCTGAAGGCCCACACGAGGACGCACACAGGAGAAAAGCCTTTCAGC
NM_005655.3         TTTAAAAGTTCCCATCTGAAGGCCCACACGAGGACGCACACAGGAGAAAAGCCTTTCAGC
NM_001032282.3      TTTAAAAGTTCCCATCTGAAGGCCCACACGAGGACGCACACAGGAGAAAAGCCTTTCAGC
                    ************************************************************

HsCD00351621        TGTAGCTGGAAAGGTTGTGAAAGGAGGTTTGCCCGTTCTGATGAACTGTCCAGACACAGG
NM_005655.3         TGTAGCTGGAAAGGTTGTGAAAGGAGGTTTGCCCGTTCTGATGAACTGTCCAGACACAGG
NM_001032282.3      TGTAGCTGGAAAGGTTGTGAAAGGAGGTTTGCCCGTTCTGATGAACTGTCCAGACACAGG
                    ************************************************************

HsCD00351621        CGAACCCACACGGGTGAGAAGAAATTTGCGTGCCCCATGTGTGACCGGCGGTTCATGAGG
NM_005655.3         CGAACCCACACGGGTGAGAAGAAATTTGCGTGCCCCATGTGTGACCGGCGGTTCATGAGG
NM_001032282.3      CGAACCCACACGGGTGAGAAGAAATTTGCGTGCCCCATGTGTGACCGGCGGTTCATGAGG
                    ************************************************************

HsCD00351621        AGTGACCATTTGACCAAGCATGCCCGGCGCCATCTATCAGCCAAGAAGCTACCAAACTGG
NM_005655.3         AGTGACCATTTGACCAAGCATGCCCGGCGCCATCTATCAGCCAAGAAGCTACCAAACTGG
NM_001032282.3      AGTGACCATTTGACCAAGCATGCCCGGCGCCATCTATCAGCCAAGAAGCTACCAAACTGG
                    ************************************************************

HsCD00351621        CAGATGGAAGTGAGCAAGCTAAATGACATTGCTCTACCTCCAACCCCTGCTCCCACACAG
NM_005655.3         CAGATGGAAGTGAGCAAGCTAAATGACATTGCTCTACCTCCAACCCCTGCTCCCACACAG
NM_001032282.3      CAGATGGAAGTGAGCAAGCTAAATGACATTGCTCTACCTCCAACCCCTGCTCCCACACAG
                    ************************************************************

HsCD00351621        TGA
NM_005655.3         TGA
NM_001032282.3      TGA
                    ***