Gene:
Gene Symbol:
KIF2A
Gene Name:
kinesin heavy chain member 2A
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR221
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100068500)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00351701
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCAACGGCCAACTTCGGCAAGATCCAGATCGGGATTTACGTGGAGATCAAGCGCAGC
GATGGCCGAATACATCAAGCAATGGTAACATCTTTAAATGAAGATAATGAAAGTGTAACT
GTTGAATGGATAGAAAATGGAGATACAAAAGGCAAAGAGATTGACCTGGAGAGCATCTTT
TCACTTAACCCTGACCTTGTTCCTGATGAAGAAATTGAACCCAGTCCAGAAACACCTCCA
CCTCCAGCATCCTCAGCCAAAGTAAACAAAATTGTAAAGAATCGACGGACTGTAGCTTCT
ATTAAGAATGACCCTCCTTCAAGAGATAATAGAGTGGTTGGTTCAGCACGTGCACGGCCC
AGTCAATTTCCTGAACAGTCTTCCTCTGCACAACAGAATGGTAGTGTTTCAGATATATCT
CCAGTTCAAGCTGCAAAAAAGGAATTTGGACCCCCTTCACGTAGAAAATCTAATTGTGTG
AAAGAAGTAGAAAAACTGCAAGAAAAACGAGAGAAAAGGAGATTGCAACAGCAAGAACTT
AGAGAAAAAAGAGCCCAGGACGTTGATGCTACAAACCCAAATTATGAAATTATGTGTATG
ATCAGAGACTTTAGAGGAAGTTTGGATTATAGACCATTAACAACAGCAGATCCTATTGAT
GAACATAGGATATGTGTGTGTGTAAGAAAACGACCACTCAATAAAAAAGAAACTCAAATG
AAAGATCTTGATGTAATCACAATTCCTAGTAAAGATGTTGTGATGGTACATGAACCAAAA
CAAAAAGTAGATTTAACAAGGTACCTAGAAAACCAAACATTTCGTTTTGATTATGCCTTT
GATGACTCAGCTCCTAATGAAATGGTTTACAGGTTTACTGCTAGACCACTAGTGGAAACT
ATATTTGAAAGGGGAATGGCTACATGCTTTGCTTATGGGCAGACTGGAAGTGGAAAAACT
CATACTATGGGTGGTGACTTTTCAGGAAAGAACCAAGATTGTTCTAAAGGAATTTATGCA
TTAGCAGCTCGAGATGTCTTTTTAATGCTAAAGAAGCCAAACTATAAGAAGCTAGAACTT
CAAGTATATGCAACCTTCTTTGAAATTTATAGTGGAAAGGTGTTTGACTTGCTAAACAGG
AAAACAAAATTAAGAGTTCTAGAAGATGGAAAACAGCAGGTTCAAGTGGTGGGATTACAG
GAACGGGAGGTCAAATGTGTTGAAGATGTACTGAAACTCATTGACATAGGCAACAGTTGC
AGAACATCCGGTCAAACATCTGCAAATGCACATTCATCTCGGAGCCATGCAGTGTTTCAG
ATTATTCTTAGAAGGAAAGGAAAACTACATGGCAAATTTTCTCTCATTGATTTGGCTGGA
AATGAAAGAGGAGCTGATACTTCCAGTGCGGACAGGCAAACTAGGCTTGAAGGTGCTGAA
ATTAATAAAAGCCTTTTAGCACTCAAGGAGTGCATCAGAGCCTTAGGTAGAAATAAACCT
CATACTCCTTTCCGTGCAAGTAAACTCACTCAGGTGTTAAGAGATTCTTTCATAGGTGAA
AACTCTCGTACCTGCATGATTGCCACAATCTCTCCAGGAATGGCATCCTGTGAAAATACT
CTTAATACATTAAGATATGCAAATAGAGTAAAGGAGTTTGGAATTAGTCCATCAGACATT
CCCTTCTCACAGGGTAGTGGCAGTCGCCCTGATCTCTCTCCTTCTTATGAATATGACGAC
TTTTCTCCTTCAGTTACCAGGGTCAAAGAATTGACTGTAGATCCAACTGCTGCTGGTGAT
GTTCGTCCAATAATGCACCATCCACCAAACCAGATTGATGACTTAGAGACACAGTGGGGT
GTGGGGAGTTCCCCTCAGAGAGATGATCTAAAACTTCTTTGTGAACAAAATGAAGAAGAA
GTCTCTCCACAGTTGTTTACTTTCCACGAAGCTGTTTCACAAATGGTAGAAATGGAAGAA
CAAGTTGTAGAAGATCACAGGGCAGTGTTCCAGGAATCTATTCGGTGGTTAGAAGATGAA
AAGGCCCTCTTAGAGATGACTGAAGAAGTAGATTATGATGTCGATTCATATGCTACACAA
CTTGAAGCTATTCTTGAGCAAAAAATAGACATTTTAACTGAACTGCGGGATAAAGTGAAA
TCTTTCCGTGCAGCTCTACAAGAGGAGGAACAAGCCAGCAAGCAAATCAACCCGAAGAGA
CCCCGTGCCCTT
Protein sequence predicted based on nucleotide sequence
MATANFGKIQIGIYVEIKRSDGRIHQAMVTSLNEDNESVTVEWIENGDTKGKEIDLESIF SLNPDLVPDEEIEPSPETPPPPASSAKVNKIVKNRRTVASIKNDPPSRDNRVVGSARARP SQFPEQSSSAQQNGSVSDISPVQAAKKEFGPPSRRKSNCVKEVEKLQEKREKRRLQQQEL REKRAQDVDATNPNYEIMCMIRDFRGSLDYRPLTTADPIDEHRICVCVRKRPLNKKETQM KDLDVITIPSKDVVMVHEPKQKVDLTRYLENQTFRFDYAFDDSAPNEMVYRFTARPLVET IFERGMATCFAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALAARDVFLMLKKPNYKKLEL QVYATFFEIYSGKVFDLLNRKTKLRVLEDGKQQVQVVGLQEREVKCVEDVLKLIDIGNSC RTSGQTSANAHSSRSHAVFQIILRRKGKLHGKFSLIDLAGNERGADTSSADRQTRLEGAE INKSLLALKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMASCENT LNTLRYANRVKEFGISPSDIPFSQGSGSRPDLSPSYEYDDFSPSVTRVKELTVDPTAAGD VRPIMHHPPNQIDDLETQWGVGSSPQRDDLKLLCEQNEEEVSPQLFTFHEAVSQMVEMEE QVVEDHRAVFQESIRWLEDEKALLEMTEEVDYDVDSYATQLEAILEQKIDILTELRDKVK SFRAALQEEEQASKQINPKRPRAL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR221
Synonyms:
pDONR221, pENTR221-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php.
Size (bp):
2574
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pUC ori
pUC origin of replication
1905
4
primer site
M13 F
M13 forward priming site
846
865
primer site
M13 Rev
M13 reverse primer site
846
865
recombination site
attL1
attL1 recombination site
575
675
recombination site
attL2
attL2 recombination site
805
706
recombination site
rrnBT1
rrnB T1 transcription termination sequence
434
477
recombination site
rrnBT2
rrnB T2 transcription termination sequence
275
302
selectable marker
KanR
kanamycin resistance gene
975
1784
