DNASU Plasmid | FRMD3 (Homo sapiens) in pENTR221 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: FRMD3

Gene Name: FERM domain containing 3

Original Clone ID:

None

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR221

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

The ORFeome Collaboration entry clone (IMAGE:100068662)

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00351899

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 639nts

Open reading frame: 1 to 639

Reference Sequence Alignment

ATGCAGGCCGAGGAGACCAAGGGGATTGGAATAACCAGTATGGCAAAATGCTTGGTCAAG
ATCCAGACTCGACGAAGCCTCCAATTGCACATGGTGAACCACTGCAACAGTAATGTGTTT
GTTCGTCTGCTGAGACTCGGCTCCAAGGTCACAGCTCGTAACACTGGTGTTCCATTGCCT
AAAGAGGAGAACATTTCTGCTCCCTTGATCTCCAGCTCCCCAGTGAAGGCAGCCCGGGAG
TATGAAGATCCCCCTAGTGAAGAGGAAGATAAAATAAAAGAAGAACCTTTAACCATCTCT
GAACTAGTGTACAACCCAAGTGCCAGCCTGCTCCCCACCCCTGTGGATGACGATGAGATT
GACATGCTCTTTGACTGTCCTTCTAGGCTTGAGTTGGAAAGAGAAGACACAGATTCATTT
GAGGATCTGGAAGCAGATGAAAACGCCTTTTTGATTGCTGAAGAAGAGGAGCTGAAGGAG
GCTCGCCGTGCTTTGTCGTGGAGCTATGACATTCTGACTGGCCATATTCGGGTGAACCCA
CTGGTCAAGAGTTTTTCCAGGCTCCTTGTGGTGGGCCTGGGACTGCTGCTCTTTGTATTT
CCCCTGCTCCTCCTCCTTTTGGAGTCAGTCTCCATGCAG

Protein sequence predicted based on nucleotide sequence

MQAEETKGIGITSMAKCLVKIQTRRSLQLHMVNHCNSNVFVRLLRLGSKVTARNTGVPLP
KEENISAPLISSSPVKAAREYEDPPSEEEDKIKEEPLTISELVYNPSASLLPTPVDDDEI
DMLFDCPSRLELEREDTDSFEDLEADENAFLIAEEEELKEARRALSWSYDILTGHIRVNP
LVKSFSRLLVVGLGLLLFVFPLLLLLLESVSMQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR221

Synonyms:

pDONR221, pENTR221-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php.

Size (bp):

2574

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

pUC origin of replication

1905

primer site

M13 F

M13 forward priming site

846

865

primer site

M13 Rev

M13 reverse primer site

846

865

recombination site

attL1

attL1 recombination site

575

675

recombination site

attL2

attL2 recombination site

805

706

recombination site

rrnBT1

rrnB T1 transcription termination sequence

434

477

recombination site

rrnBT2

rrnB T2 transcription termination sequence

275

302

selectable marker

KanR

kanamycin resistance gene

975

1784

Gene

Gene Symbol :

FRMD3

Symbol Nomenclature :

FRMD3

SYNONYM :

4.1O; EPB41L4O; EPB41LO; P410

Designation :

FERM domain-containing protein 3|band 4.1-like protein 4|band 4.1-like protein 4O|ovary type protein 4.1|protein 4.1O

Full Nomenclature :

FERM domain containing 3

GENEID :

257019

Locus Tag :

RP11-439K3.2

GenBank Accession :

564506

HGNC :

24125

MIM :

607619

Vega :

OTTHUMG00000020103

Target GenBank:

GQ129452

Reference Sequence Alignment

  

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      ATGTTCGCCTCCTGCCACTGTGTGCCGAGAGGCAGGAGGACCATGAAAATGATCCACTTT
NM_001244961.1      ------------------------------------------------------------
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      CGGAGCTCCAGCGTCAAATCGCTCAGCCAGGAGATGAGATGCACCATCCGGCTGCTGGAC
NM_001244961.1      ------------------------------------------------------------
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      GACTCGGAGATCTCCTGCCACATCCAGAGGGAAACCAAAGGGCAGTTTCTCATTGACCAC
NM_001244961.1      ------------------------------------------------------------
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      ATCTGCAACTACTACAGCCTGCTGGAGAAGGACTACTTTGGCATTCGCTATGTGGACCCA
NM_001244961.1      ------------------------------------------------------------
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      GAGAAGCAAAGGCACTGGCTTGAACCTAACAAGTCCATCTTCAAGCAAATGAAAACTCAT
NM_001244961.1      ------------------------------------------------------------
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      CCACCATACACCATGTGCTTTAGAGTGAAATTCTACCCACATGAACCCTTGAAGATTAAA
NM_001244961.1      ------------------------------------------------------------
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      GAAGAGCTCACAAGATACCTTTTATACCTTCAGATTAAAAGGGACATTTTTCATGGCCGC
NM_001244961.1      ------------------------------------------------------------
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      CTGCTGTGCTCCTTTTCTGATGCTGCCTACCTGGGTGCCTGTATTGTTCAAGCTGAGCTT
NM_001244961.1      ------------------------------------------------------------
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      GGTGATTACGATCCTGATGAGCATCCTGAGAATTACATCAGTGAGTTTGAGATTTTCCCC
NM_001244961.1      ------------------------------------------------------------
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      AAGCAGTCACAGAAGCTGGAAAGAAAAATAGTGGAAATTCATAAAAATGAACTCAGGGGG
NM_001244961.1      ------------------------------------------ATGGTGTTCAGAAGGGGG
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      CAGAGCCCACCAGTTGCTGAATTTAACTTGCTCCTGAAAGCTCACACTTTGGAAACCTAC
NM_001244961.1      CAGAGCCCACCAGTTGCTGAATTTAACTTGCTCCTGAAAGCTCACACTTTGGAAACCTAC
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      GGGGTGGATCCTCACCCATGCAAGGATTCAACAGGCACAACAACATTTTTAGGATTCACA
NM_001244961.1      GGGGTGGATCCTCACCCATGCAAGGATTCAACAGGCACAACAACATTTTTAGGATTCACA
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      GCTGCAGGCTTTGTGGTCTTTCAGGGAAATAAGAGAATCCATTTGATAAAATGGCCAGAT
NM_001244961.1      GCTGCAGGCTTTGTGGTCTTTCAGGGAAATAAGAGAATCCATTTGATAAAATGGCCAGAT
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      GTCTGCAAATTGAAGTTTGAAGGGAAGACATTTTATGTGATTGGCACCCAGAAGGAGAAA
NM_001244961.1      GTCTGCAAATTGAAGTTTGAAGGGAAGACATTTTATGTGATTGGCACCCAGAAGGAGAAA
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      AAAGCCATGTTGGCATTCCATACTTCAACACCAGCTGCCTGCAAACATCTTTGGAAGTGT
NM_001244961.1      AAAGCCATGTTGGCATTCCATACTTCAACACCAGCTGCCTGCAAACATCTTTGGAAGTGT
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ------------------------------------------------------------
NM_001244959.1      GGAGTGGAAAACCAGGCCTTTTATAAGTATGCAAAATCCAGTCAGATCAAGACTGTATCA
NM_001244961.1      GGAGTGGAAAACCAGGCCTTTTATAAGTATGCAAAATCCAGTCAGATCAAGACTGTATCA
NM_001244962.1      ------------------------------------------------------------
                                                                                

HsCD00351899        ----------------ATGCAGGCCGAGGAGACCAAGGGGATTGGAATAACCAGTATGGC
NM_001244959.1      AGCAGCAAGATATTTTTTAAAGGAAGTAGATTTCGATATAGTGGGAAAGTTGCCAAAGAG
NM_001244961.1      AGCAGCAAGATATTTTTTAAAGGAAGTAGATTTCGATATAGTGGGAAAGTTGCCAAAGAG
NM_001244962.1      ----------------ATGCAGGCCGAGGAGACCAAGGGGATTGGAATAACCAGTATGGC
                                    :*..***..*:.** : *.* . ..* ****:.:  . :*:*. 

HsCD00351899        AAAATGCTTGGTCAAGATCCAGACTCGACGAAGCCTCCAATTGCACATGGTGAACCACTG
NM_001244959.1      GTGGTGGAGGCCAGTTCCAAGATCCAGAGGGAGCCTCCTGAGGTGCACAGAGCCAACATT
NM_001244961.1      GTGGTGGAGGCCAGTTCCAAGATCCAGAGGGAGCCTCCTGAGGTGCACAGAGCCAACATT
NM_001244962.1      AAAATGCTTGGTCAAGATCCAGACTCGACGAAGCCTCCAATTGCACATGGTGAACCACTG
                    .:..** : *  ..: . ....:* .** *.*******:.: * .** .*:*......* 

HsCD00351899        CAACAGTAATGTGTTTGTTCGTCTGCTGAGACTCGGCTCCAAGGTCACAG----------
NM_001244959.1      ACTCAGAGCCGCAGTTCCCACTCCTTGAACAAACAGCTCATCATTAACATGGAACCCCTG
NM_001244961.1      ACTCAGAGCCGCAGTTCCCACTCCTTGAACAAACAGCTCATCATTAACATGGAACCCCTG
NM_001244962.1      CAACAGTAATGTGTTTGTTCGTCTGCTGAGACTCGGCTCCAAGGTCACAG----------
                    ..:***:.. * . **   . **    .* *.:*.****.:.. *.***           

HsCD00351899        -------------------------------------------CTCGTAACACTGGTGTT
NM_001244959.1      CAGCCCCTGCTTCCTTCCCCCAGCGAGCAAGAAGAAGAACTTCCTCTGGGTGAGGGTGTT
NM_001244961.1      CAGCCCCTGCTTCCTTCCCCCAGCGAGCAAGAAGAAGAACTTCCTCTGGGTGAGGGTGTT
NM_001244962.1      -------------------------------------------CTCGTAACACTGGTGTT
                                                               ***  .. .. ******

HsCD00351899        CCATTGCCTAAAGAGGAGAACATTTCTGCTCCCTTGATCTCCAGCTCCCCAGTGAAGGCA
NM_001244959.1      CCATTGCCTAAAGAGGAGAACATTTCTGCTCCCTTGATCTCCAGCTCCCCAGTGAAGGCA
NM_001244961.1      CCATTGCCTAAAGAGGAGAACATTTCTGCTCCCTTGATCTCCAGCTCCCCAGTGAAGGCA
NM_001244962.1      CCATTGCCTAAAGAGGAGAACATTTCTGCTCCCTTGATCTCCAGCTCCCCAGTGAAGGCA
                    ************************************************************

HsCD00351899        GCCCGGGAGTATGAAGATCCCCCTAGTGAAGAGGAAGATAAAATAAAAGAAGAACCTTTA
NM_001244959.1      GCCCGGGAGTATGAAGATCCCCCTAGTGAAGAGGAAGATAAAATAAAAGAAGAACCTTTA
NM_001244961.1      GCCCGGGAGTATGAAGATCCCCCTAGTGAAGAGGAAGATAAAATAAAAGAAGAACCTTTA
NM_001244962.1      GCCCGGGAGTATGAAGATCCCCCTAGTGAAGAGGAAGATAAAATAAAAGAAGAACCTTTA
                    ************************************************************

HsCD00351899        ACCATCTCTGAACTAGTGTACAACCCAAGTGCCAGCCTGCTCCCCACCCCTGTGGATGAC
NM_001244959.1      ACCATCTCTGAACTAGTGTACAACCCAAGTGCCAGCCTGCTCCCCACCCCTGTGGATGAC
NM_001244961.1      ACCATCTCTGAACTAGTGTACAACCCAAGTGCCAGCCTGCTCCCCACCCCTGTGGATGAC
NM_001244962.1      ACCATCTCTGAACTAGTGTACAACCCAAGTGCCAGCCTGCTCCCCACCCCTGTGGATGAC
                    ************************************************************

HsCD00351899        GATGAGATTGACATGCTCTTTGACTGTCCTTCTAGGCTTGAGTTGGAAAGAGAAGACACA
NM_001244959.1      GATGAGATTGACATGCTCTTTGACTGTCCTTCTAGGCTTGAGTTGGAAAGAGAAGACACA
NM_001244961.1      GATGAGATTGACATGCTCTTTGACTGTCCTTCTAGGCTTGAGTTGGAAAGAGAAGACACA
NM_001244962.1      GATGAGATTGACATGCTCTTTGACTGTCCTTCTAGGCTTGAGTTGGAAAGAGAAGACACA
                    ************************************************************

HsCD00351899        GATTCATTTGAGGATCTGGAAGCAGATGAAAACGCCTTTTTGATTGCTGAAGAAGAGGAG
NM_001244959.1      GATTCATTTGAGGATCTGGAAGCAGATGAAAACGCCTTTTTGATTGCTGAAGAAGAGGAG
NM_001244961.1      GATTCATTTGAGGATCTGGAAGCAGATGAAAACGCCTTTTTGATTGCTGAAGAAGAGGAG
NM_001244962.1      GATTCATTTGAGGATCTGGAAGCAGATGAAAACGCCTTTTTGATTGCTGAAGAAGAGGAG
                    ************************************************************

HsCD00351899        CTGAAGGAGGCTCGCCGTGCTTTGTCGTGGAGCTATGACATTCTGACTGGCCATATTCGG
NM_001244959.1      CTGAAGGAGGCTCGCCGTGCTTTGTCGTGGAGCTATGACATTCTGACTGGCCATATTCGG
NM_001244961.1      CTGAAGGAGGCTCGCCGTGCTTTGTCGTGGAGCTATGACATTCTGACTGGCCATATTCGG
NM_001244962.1      CTGAAGGAGGCTCGCCGTGCTTTGTCGTGGAGCTATGACATTCTGACTGGCCATATTCGG
                    ************************************************************

HsCD00351899        GTGAACCCACTGGTCAAGAGTTTTTCCAGGCTCCTTGTGGTGGGCCTGGGACTGCTGCTC
NM_001244959.1      GTGAACCCACTGGTCAAGAGTTTTTCCAGGCTCCTTGTGGTGGGCCTGGGACTGCTGCTC
NM_001244961.1      GTGAACCCACTGGTCAAGAGTTTTTCCAGGCTCCTTGTGGTGGGCCTGGGACTGCTGCTC
NM_001244962.1      GTGAACCCACTGGTCAAGAGTTTTTCCAGGCTCCTTGTGGTGGGCCTGGGACTGCTGCTC
                    ************************************************************

HsCD00351899        TTTGTATTTCCCCTGCTCCTCCTCCTTTTGGAGTCAGTCTCCATGCAG---
NM_001244959.1      TTTGTATTTCCCCTGCTCCTCCTCCTTTTGGAGTCAGTCTCCATGCAGTAA
NM_001244961.1      TTTGTATTTCCCCTGCTCCTCCTCCTTTTGGAGTCAGTCTCCATGCAGTAA
NM_001244962.1      TTTGTATTTCCCCTGCTCCTCCTCCTTTTGGAGTCAGTCTCCATGCAGTAA
                    ************************************************

Protein

UniProt :

A2A2Y4

HPRD :

07402

Clone

Cloning Information :

564506

Organism

Species Specific ID:

257019

Genome

Chromosome :

Map Location :

9q21.32

Ensembl :

ENSG00000172159

Reagents

Labome :

FRMD3-antibody