Gene:
Gene Symbol:
CACTIN
Gene Name:
cactin, spliceosome C complex subunit
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069243)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00353977
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGGTCGGGACACACGCTCGCGCTCGCGGTCCGCGGGTCGCCGGGGCCGAAGGCGGCAG
AGTCAGAGCGGGAGCCGAAGTCGGAGCAGGAGCCATGGGCGGCGAAACCGACGGCGCCGG
GAGGACGAGGGACGGCGCAGACGGAGGCGGCGGAGCCGGGAGCGCAGGTCAGATTCAGAG
GAAGAGCGGTGGCAGCGCTCAGGGATGCGAAGCCGGAGCCCCCCGCGGCCCAAGTGGCAC
TCAAGAGATGGGTCCTCTCAGTCGGACTCAGGAGAGGAGCAGTCACGGGGCCAGTGGGCT
CGCCGGCGACGGCGCGCACGCTCGTGGTCTCCTAGCTCCTCAGCATCCAGCTCCGCGTCT
CCAGGGCGATCCCAGAGCCCCCGGGCGGCCGCGGCTGCCCTGAGCCAGCAGCAGAGCCTG
CAGGAGCGGCTGCGGCTGCGGGAGGAGCGGAAGCAGCAGGAGGAGCTGATGAAGGCCTTC
GAGACGCCCGAGGAGAAGCGCGCACGGCGGCTGGCCAAGAAGGAGGCCAAGGAGCGCAAG
AAGCGGGAGAAGATGGGCTGGGGTGAGGAGTACATGGGCTACACCAACACCGACAACCCC
TTCGGAGACAACAACCTGCTGGGCACCTTCATCTGGAATAAGGCCCTGGAGAAGAAGGGG
ATCAGCCACCTGGAGGAGAAGGAGCTGAAGGAGCGGAACAAGAGGATCCAGGAGGACAAC
CGGCTGGAGCTGCAGAAGGTGAAGCAGCTGCGGCTGGAGCGGGAGCGGGAGAAGGCCATG
CGCGAGCAGGAGCTGGAGATGCTGCAGCGCGAGAAGGAGGCAGAGCACTTCAAGACATGG
GAGGAGCAGGAGGACAACTTCCACCTCCAGCAGGCCAAGCTGCGTTCCAAGATCCGCATC
CGGGACGGGCGGGCCAAGCCCATCGACCTGCTGGCCAAGTACATCAGCGCTGAGGATGAC
GATCTGGCCGTGGAGATGCATGAGCCCTACACGTTCCTCAACGGCCTCACCGTGGCCGAC
ATGGAGGACCTGCTGGAGGATATCCAGGTCTACATGGAGCTGGAGCAGGGCAAGAACGCC
GACTTCTGGCGGGACATGACCACCATCACCGAGGACGAGATCTCCAAGCTCCGCAAGCTG
GAGGCCTCGGGCAAGGGGCCAGGTGAGCGCCGCGAGGGGGTCAACGCCTCCGTCAGCTCT
GATGTGCAGTCGGTGTTCAAGGGGAAGACATACAACCAGCTGCAGGTCATCTTCCAGGGC
ATCGAGGGCAAAATCCGCGCTGGTGGCCCCAACCTGGACATGGGCTACTGGGAGAGCCTC
CTGCAGCAGCTTCGTGCCCACATGGCACGGGCCCGGCTGCGTGAGCGCCACCAGGACGTG
CTGCGGCAGAAGCTGTACAAACTGAAGCAGGAGCAGGGCGTGGAGAGCGAGCCGCTGTTC
CCCATCCTCAAGCAGGAGCCCCAGTCCCCCAGCCGCAGCCTGGAGCCTGAGGACGCGGCG
CCCACCCCGCCCGGGCCCTCCTCGGAGGGCGGCCCCGCGGAGGCCGAGGTGGACGGCGCG
ACCCCGACAGAGGGCGACGGCGACGGGGACGGTGAGGGCGAGGGCGAGGGCGAGGCGGTG
CTCATGGAGGAGGACCTGATCCAGCAGAGCCTGGACGACTACGACGCCGGCAGGTACAGC
CCGCGGCTGCTCACGGCGCACGAGCTGCCACTGGACGCGCACGTGCTGGAACCGGATGAG
GACCTGCAGCGCCTGCAGCTCTCGCGCCAGCAGCTCCAGGTCACGGGAGACGCCAGCGAG
AGCGCCGAGGACATCTTCTTCCGGCGGGCCAAGGAGGGCATGGGCCAGGACGAGGCGCAG
TTCAGCGTGGAGATGCCACTCACCGGCAAGGCCTACCTGTGGGCCGACAAGTACCGGCCA
CGCAAGCCGCGCTTCTTCAACCGCGTGCACACGGGCTTCGAGTGGAACAAGTACAACCAG
ACGCACTACGACTTTGACAACCCACCGCCCAAGATCGTGCAGGGATACAAGTTCAACATC
TTCTACCCCGACCTCATCGACAAGCGCTCCACGCCCGAGTACTTCCTGGAGGCCTGCGCC
GACAACAAGGATTTCGCCATCCTGCGCTTCCACGCGGGGCCGCCCTACGAGGACATCGCT
TTCAAGATCGTCAACCGCGAGTGGGAATACTCGCACCGCCACGGCTTCCGCTGCCAGTTT
GCCAACGGCATCTTCCAGCTGTGGTTTCACTTCAAGCGCTACCGCTATCGGCGG
Protein sequence predicted based on nucleotide sequence
MGRDTRSRSRSAGRRGRRRQSQSGSRSRSRSHGRRNRRRREDEGRRRRRRRSRERRSDSE EERWQRSGMRSRSPPRPKWHSRDGSSQSDSGEEQSRGQWARRRRRARSWSPSSSASSSAS PGRSQSPRAAAAALSQQQSLQERLRLREERKQQEELMKAFETPEEKRARRLAKKEAKERK KREKMGWGEEYMGYTNTDNPFGDNNLLGTFIWNKALEKKGISHLEEKELKERNKRIQEDN RLELQKVKQLRLEREREKAMREQELEMLQREKEAEHFKTWEEQEDNFHLQQAKLRSKIRI RDGRAKPIDLLAKYISAEDDDLAVEMHEPYTFLNGLTVADMEDLLEDIQVYMELEQGKNA DFWRDMTTITEDEISKLRKLEASGKGPGERREGVNASVSSDVQSVFKGKTYNQLQVIFQG IEGKIRAGGPNLDMGYWESLLQQLRAHMARARLRERHQDVLRQKLYKLKQEQGVESEPLF PILKQEPQSPSRSLEPEDAAPTPPGPSSEGGPAEAEVDGATPTEGDGDGDGEGEGEGEAV LMEEDLIQQSLDDYDAGRYSPRLLTAHELPLDAHVLEPDEDLQRLQLSRQQLQVTGDASE SAEDIFFRRAKEGMGQDEAQFSVEMPLTGKAYLWADKYRPRKPRFFNRVHTGFEWNKYNQ THYDFDNPPPKIVQGYKFNIFYPDLIDKRSTPEYFLEACADNKDFAILRFHAGPPYEDIA FKIVNREWEYSHRHGFRCQFANGIFQLWFHFKRYRYRR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
