DNASU Plasmid | C20orf54 (H. sapiens )<br>In pANT7_cGST (GST-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: SLC52A3

Gene Name: solute carrier family 52 (riboflavin transporter), member 3

Map: pANT7_cGST.doc

Original Clone ID:

None

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pANT7_cGST

Format:

FUSION

Source:

Center for Personalized Diagnostics

Description:

None

Comments:

generated from ORFeome entry clone (IMAGE:100071686)

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Center for Personalized Diagnostics

HsCD00357526

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1407nts

Open reading frame: 1 to 1407

Reference Sequence Alignment

ATGGCCTTCCTGATGCACCTGCTGGTCTGCGTCTTCGGAATGGGCTCCTGGGTGACCATC
AATGGGCTCTGGGTAGAGCTGCCCCTGCTGGTGATGGAGCTGCCCGAGGGCTGGTACCTG
CCCTCCTACCTCACGGTGGTCATCCAGCTGGCCAACATCGGGCCCCTCCTGGTCACCCTG
CTCCATCACTTCCGGCCCAGCTGCCTTTCCGAAGTGCCCATCATCTTCACCCTGCTGGGC
GTGGGAACCGTCACCTGCATCATCTTTGCCTTCCTCTGGAATATGACCTCCTGGGTGCTG
GACGGCCACCACAGCATCGCTTTCTTGGTCCTCACCTTCTTCCTGGCCCTGGTGGACTGC
ACCTCTTCAGTGACCTTCCTGCCGTTCATGAGCCGGCTGCCCACCTACTACCTCACCACC
TTCTTTGTGGGTGAAGGACTCAGCGGCCTCTTGCCCGCCCTGGTGGCTCTTGCCCAGGGC
TCCGGTCTCACTACCTGCGTCAATGTCACTGAGATATCAGACAGCGTACCAAGCCCTGTA
CCCACGAGGGAGACTGACATCGCACAGGGAGTTCCCAGAGCTTTGGTGTCCGCCCTCCCC
GGAATGGAAGCACCCTTGTCCCACCTGGAGAGCCGCTACCTTCCCGCCCACTTCTCACCC
CTGGTCTTCTTCCTCCTCCTATCCATCATGATGGCCTGCTGCCTCGTGGCGTTCTTTGTC
CTCCAGCGTCAACCCAGGTGCTGGGAGGCTTCCGTGGAAGACCTCCTCAATGACCAGGTC
ACCCTCCACTCCATCCGGCTGCGGGAAGAGAATGACTTGGGCCCTGCAGGCATGGTGGAC
AGCAGCCAGGGCCAGGGGTATCTAGAGGAGAAAGCAGCCCCCTGCTGCCCGGCGCACCTG
GCCTTCGTCTATACCCTGGTGGCCTTCGTCAACGCGCTCACCAACGGCATGCTGCCCTCT
GTGCAGACCTACTCCTGCCTGTCCTATGGGCCAGTTGCCTACCACCTGGCTGCCACCCTC
AGCATTGTGGCCAACCCTCTTGCCTCGTTGGTCTCCATGTTCCTGCCTAACAGGTCTCTG
CTGTTCCTGGGGGTCCTCTCCGTGCTTGGGACCTGCTTTGGGGGCTACAACATGGCCATG
GCGGTGATGAGCCCCTGCCCCCTCTTGCAGGGCCACTGGGGTGGGGAAGTCCTCATTGTG
GCCTCGTGGGTGCTTTTCAGCGGCTGCCTCAGCTACGTCAAGGTGATGCTGGGCGTGGTC
CTGCGCGACCTCAGCCGCAGCGCCCTCTTGTGGTGCGGGGCGGCGGTGCAGCTGGGCTCG
CTGCTCGGAGCGCTGCTCATGTTCCCTCTGGTCAACGTGCTGCGGCTCTTCTCGTCCGCG
GACTTCTGCAATCTGCACTGTCCAGCC

Protein sequence predicted based on nucleotide sequence

MAFLMHLLVCVFGMGSWVTINGLWVELPLLVMELPEGWYLPSYLTVVIQLANIGPLLVTL
LHHFRPSCLSEVPIIFTLLGVGTVTCIIFAFLWNMTSWVLDGHHSIAFLVLTFFLALVDC
TSSVTFLPFMSRLPTYYLTTFFVGEGLSGLLPALVALAQGSGLTTCVNVTEISDSVPSPV
PTRETDIAQGVPRALVSALPGMEAPLSHLESRYLPAHFSPLVFFLLLSIMMACCLVAFFV
LQRQPRCWEASVEDLLNDQVTLHSIRLREENDLGPAGMVDSSQGQGYLEEKAAPCCPAHL
AFVYTLVAFVNALTNGMLPSVQTYSCLSYGPVAYHLAATLSIVANPLASLVSMFLPNRSL
LFLGVLSVLGTCFGGYNMAMAVMSPCPLLQGHWGGEVLIVASWVLFSGCLSYVKVMLGVV
LRDLSRSALLWCGAAVQLGSLLGALLMFPLVNVLRLFSSADFCNLHCPA

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pANT7_cGST

Synonyms:

pANT7-cGST

Sequencing Primer:

Forward: NAP150

Reverse: NAP138R

Description:

with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial

Comments:

One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems

Size (bp):

5964

Parent Vector:

None

Properties:

Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker

Author Name:

Joshua LaBaer
Al Lau
Niroshan Ramachandran

Publications:

PMID: 15232106

Title: Self-assembling protein microarrays

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

ColE1 bacterial origin of replication

3931

3249

death cassette

ccdB

ccdB death cassette present in 'empty vector' form only (replace with gene of interest)

1876

2085

primer

NAP138

NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?

2405

2384

primer

NAP150

Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?

388

408

promoter

T7 transcriptional start sequence

5940

5964

recombination site

attR1

AttR1 site for recombination in empty vector

549

565

recombination site

attR2

AttR2 site for recombination in empty vector

2236

2251

selectable marker

CmR

chloramphenicol resistance

782

1438

selectable marker

AmpR

ampicillin resistance gene

4688

4029

tag

GST

Glutathione transferase (GST) tag

2282

2950

Gene

Gene Symbol :

SLC52A3

Symbol Nomenclature :

SLC52A3

SYNONYM :

BVVLS; BVVLS1; C20orf54; RFT2; RFVT3; bA371L19.1; hRFT2

Designation :

riboflavin transporter 2|solute carrier family 52 member 3|solute carrier family 52, riboflavin transporter, member 3

Full Nomenclature :

solute carrier family 52 (riboflavin transporter), member 3

GENEID :

113278

GenBank Accession :

569814

HGNC :

16187

MIM :

613350

Vega :

OTTHUMG00000031647

Target GenBank:

HQ448283

Reference Sequence Alignment

  

HsCD00357526      ATGGCCTTCCTGATGCACCTGCTGGTCTGCGTCTTCGGAATGGGCTCCTGGGTGACCATC
NM_033409.3       ATGGCCTTCCTGATGCACCTGCTGGTCTGCGTCTTCGGAATGGGCTCCTGGGTGACCATC
                  ************************************************************

HsCD00357526      AATGGGCTCTGGGTAGAGCTGCCCCTGCTGGTGATGGAGCTGCCCGAGGGCTGGTACCTG
NM_033409.3       AATGGGCTCTGGGTAGAGCTGCCCCTGCTGGTGATGGAGCTGCCCGAGGGCTGGTACCTG
                  ************************************************************

HsCD00357526      CCCTCCTACCTCACGGTGGTCATCCAGCTGGCCAACATCGGGCCCCTCCTGGTCACCCTG
NM_033409.3       CCCTCCTACCTCACGGTGGTCATCCAGCTGGCCAACATCGGGCCCCTCCTGGTCACCCTG
                  ************************************************************

HsCD00357526      CTCCATCACTTCCGGCCCAGCTGCCTTTCCGAAGTGCCCATCATCTTCACCCTGCTGGGC
NM_033409.3       CTCCATCACTTCCGGCCCAGCTGCCTTTCCGAAGTGCCCATCATCTTCACCCTGCTGGGC
                  ************************************************************

HsCD00357526      GTGGGAACCGTCACCTGCATCATCTTTGCCTTCCTCTGGAATATGACCTCCTGGGTGCTG
NM_033409.3       GTGGGAACCGTCACCTGCATCATCTTTGCCTTCCTCTGGAATATGACCTCCTGGGTGCTG
                  ************************************************************

HsCD00357526      GACGGCCACCACAGCATCGCTTTCTTGGTCCTCACCTTCTTCCTGGCCCTGGTGGACTGC
NM_033409.3       GACGGCCACCACAGCATCGCCTTCTTGGTCCTCACCTTCTTCCTGGCCCTGGTGGACTGC
                  ******************** ***************************************

HsCD00357526      ACCTCTTCAGTGACCTTCCTGCCGTTCATGAGCCGGCTGCCCACCTACTACCTCACCACC
NM_033409.3       ACCTCTTCAGTGACCTTCCTGCCGTTCATGAGCCGGCTGCCCACCTACTACCTCACCACC
                  ************************************************************

HsCD00357526      TTCTTTGTGGGTGAAGGACTCAGCGGCCTCTTGCCCGCCCTGGTGGCTCTTGCCCAGGGC
NM_033409.3       TTCTTTGTGGGTGAAGGACTCAGCGGCCTCTTGCCCGCCCTGGTGGCTCTTGCCCAGGGC
                  ************************************************************

HsCD00357526      TCCGGTCTCACTACCTGCGTCAATGTCACTGAGATATCAGACAGCGTACCAAGCCCTGTA
NM_033409.3       TCCGGTCTCACTACCTGCGTCAATGTCACTGAGATATCAGACAGCGTACCAAGCCCTGTA
                  ************************************************************

HsCD00357526      CCCACGAGGGAGACTGACATCGCACAGGGAGTTCCCAGAGCTTTGGTGTCCGCCCTCCCC
NM_033409.3       CCCACGAGGGAGACTGACATCGCACAGGGAGTTCCCAGAGCTTTGGTGTCCGCCCTCCCC
                  ************************************************************

HsCD00357526      GGAATGGAAGCACCCTTGTCCCACCTGGAGAGCCGCTACCTTCCCGCCCACTTCTCACCC
NM_033409.3       GGAATGGAAGCACCCTTGTCCCACCTGGAGAGCCGCTACCTTCCCGCCCACTTCTCACCC
                  ************************************************************

HsCD00357526      CTGGTCTTCTTCCTCCTCCTATCCATCATGATGGCCTGCTGCCTCGTGGCGTTCTTTGTC
NM_033409.3       CTGGTCTTCTTCCTCCTCCTATCCATCATGATGGCCTGCTGCCTCGTGGCGTTCTTTGTC
                  ************************************************************

HsCD00357526      CTCCAGCGTCAACCCAGGTGCTGGGAGGCTTCCGTGGAAGACCTCCTCAATGACCAGGTC
NM_033409.3       CTCCAGCGTCAACCCAGGTGCTGGGAGGCTTCCGTGGAAGACCTCCTCAATGACCAGGTC
                  ************************************************************

HsCD00357526      ACCCTCCACTCCATCCGGCTGCGGGAAGAGAATGACTTGGGCCCTGCAGGCATGGTGGAC
NM_033409.3       ACCCTCCACTCCATCCGGCCGCGGGAAGAGAATGACTTGGGCCCTGCAGGCACGGTGGAC
                  ******************* ******************************** *******

HsCD00357526      AGCAGCCAGGGCCAGGGGTATCTAGAGGAGAAAGCAGCCCCCTGCTGCCCGGCGCACCTG
NM_033409.3       AGCAGCCAGGGCCAGGGGTATCTAGAGGAGAAAGCAGCCCCCTGCTGCCCGGCGCACCTG
                  ************************************************************

HsCD00357526      GCCTTCGTCTATACCCTGGTGGCCTTCGTCAACGCGCTCACCAACGGCATGCTGCCCTCT
NM_033409.3       GCCTTCATCTATACCCTGGTGGCCTTCGTCAACGCGCTCACCAACGGCATGCTGCCCTCT
                  ******.*****************************************************

HsCD00357526      GTGCAGACCTACTCCTGCCTGTCCTATGGGCCAGTTGCCTACCACCTGGCTGCCACCCTC
NM_033409.3       GTGCAGACCTACTCCTGCCTGTCCTATGGGCCAGTTGCCTACCACCTGGCTGCCACCCTC
                  ************************************************************

HsCD00357526      AGCATTGTGGCCAACCCTCTTGCCTCGTTGGTCTCCATGTTCCTGCCTAACAGGTCTCTG
NM_033409.3       AGCATTGTGGCCAACCCTCTTGCCTCGTTGGTCTCCATGTTCCTGCCTAACAGGTCTCTG
                  ************************************************************

HsCD00357526      CTGTTCCTGGGGGTCCTCTCCGTGCTTGGGACCTGCTTTGGGGGCTACAACATGGCCATG
NM_033409.3       CTGTTCCTGGGGGTCCTCTCCGTGCTTGGGACCTGCTTTGGGGGCTACAACATGGCCATG
                  ************************************************************

HsCD00357526      GCGGTGATGAGCCCCTGCCCCCTCTTGCAGGGCCACTGGGGTGGGGAAGTCCTCATTGTG
NM_033409.3       GCGGTGATGAGCCCCTGCCCCCTCTTGCAGGGCCACTGGGGTGGGGAAGTCCTCATTGTG
                  ************************************************************

HsCD00357526      GCCTCGTGGGTGCTTTTCAGCGGCTGCCTCAGCTACGTCAAGGTGATGCTGGGCGTGGTC
NM_033409.3       GCCTCGTGGGTGCTTTTCAGCGGCTGCCTCAGTTACGTCAAGGTGATGCTGGGCGTGGTC
                  ******************************** ***************************

HsCD00357526      CTGCGCGACCTCAGCCGCAGCGCCCTCTTGTGGTGCGGGGCGGCGGTGCAGCTGGGCTCG
NM_033409.3       CTGCGCGACCTCAGCCGCAGCGCCCTCTTGTGGTGCGGGGCGGCGGTGCAGCTGGGCTCG
                  ************************************************************

HsCD00357526      CTGCTCGGAGCGCTGCTCATGTTCCCTCTGGTCAACGTGCTGCGGCTCTTCTCGTCCGCG
NM_033409.3       CTGCTCGGAGCGCTGCTCATGTTCCCTCTGGTCAACGTGCTGCGGCTCTTCTCGTCCGCG
                  ************************************************************

HsCD00357526      GACTTCTGCAATCTGCACTGTCCAGCC---
NM_033409.3       GACTTCTGCAATCTGCACTGTCCAGCCTAG
                  ***************************

Pathways

Reactome :

Defective AMN causes hereditary megaloblastic anemia 1

Reactome :

Defective BTD causes biotidinase deficiency

Reactome :

Defective CD320 causes methylmalonic aciduria

Reactome :

Defective CUBN causes hereditary megaloblastic anemia 1

Reactome :

Defective GIF causes intrinsic factor deficiency

Reactome :

Defective HLCS causes multiple carboxylase deficiency

Reactome :

Defective LMBRD1 causes methylmalonic aciduria and homocystinuria type cblF

Reactome :

Defective MMAA causes methylmalonic aciduria type cblA

Reactome :

Defective MMAB causes methylmalonic aciduria type cblB

Reactome :

Defective MMACHC causes methylmalonic aciduria and homocystinuria type cblC

Reactome :

Defective MMADHC causes methylmalonic aciduria and homocystinuria type cblD

Reactome :

Defective MTR causes methylmalonic aciduria and homocystinuria type cblG

Reactome :

Defective MTRR causes methylmalonic aciduria and homocystinuria type cblE

Reactome :

Defective MUT causes methylmalonic aciduria mut type

Reactome :

Defective TCN2 causes hereditary megaloblastic anemia

Reactome :

Defects in biotin (Btn) metabolism

Reactome :

Defects in cobalamin (B12) metabolism

Reactome :

Defects in vitamin and cofactor metabolism

Reactome :

Disease

Reactome :

Metabolism

Reactome :

Metabolism of vitamins and cofactors

Reactome :

Metabolism of water-soluble vitamins and cofactors

Reactome :

Vitamin B2 (riboflavin) metabolism

Protein

UniProt :

Q9NQ40

HPRD :

09843

Clone

Cloning Information :

569814

Organism

Species Specific ID:

113278

Genome

Chromosome :

Map Location :

20p13

Ensembl :

ENSG00000101276

Reagents

Labome :

SLC52A3-antibody

C20orf54 (H. sapiens )In pANT7_cGST (GST-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Organism

Genome

Reagents

C20orf54 (H. sapiens )
In pANT7_cGST (GST-tagged
In vitro expression vector)