Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
AF1011_1_134
Keyword:
None
Species:
Archaeoglobus fulgidus DSM 4304
Type:
cDNA
Vector Name:
p15Tv-LIC
Format:
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Toronto
PSI
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
AfCD00370652
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 405nts
Open reading frame: 51 to 455
ATCATCATCATCATCACAGCAGCGGCAGAGAAAACTTGTATTTCCAGGGCGTGCTCCTAC
ACTCTTTCTCTCGATTTAAGCTTTTGCGGGGATTTTTATCTCTCGCCGCTGCGAATTATG
CGATGAAACATCTACCGCTGTTTTCTACGGCAGTGGAAAACTTCGCACTATTCCCAAGGC
TCCCCAACTCAACCCAAGCCGAAGCCTTTTTAACTTCCCGCTCCTTAATGCTGAGAGTGG
CAAGATGTGATGTTTGCGGTGAGGAGGTTACAATCCCCTTCAAATGTAAGTACTGCGGCG
GAACCTTCTGCGCAGAGCACAGGCTGCCCGAGAACCACGATTGCGACGGGCTGGATGAAT
ACTGGAATGTTCCCGTTAATGTTAAGCGGAGGTTGAGCCCCCAGCCTGCAAGAAGGAGCA
GGAACATTGGATTGATGAAGTATGGGGCAAACTGATGACGAAGCTTGCGGCCGCACTCGA
GGATCCGGCTGCTAACAAAGCCCGAA
Protein sequence predicted based on nucleotide sequence
VLLHSFSRFKLLRGFLSLAAANYAMKHLPLFSTAVENFALFPRLPNSTQAEAFLTSRSLM LRVARCDVCGEEVTIPFKCKYCGGTFCAEHRLPENHDCDGLDEYWNVPVNVKRRLSPQPA RRSRNIGLMKYGAN*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Tested
SolubleProtein: Protein _Soluble
ProteinPurified: Protein_Purified
Find experimental protocols in AF1011_1_134
Recommended expression in:
Not Applicable
Vector Name:
p15Tv-LIC
Synonyms:
''
Description:
Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; ampicillin resistance; recombinational cloning (InFusion, BseRI).
Comments:
None
Size (bp):
7746
Parent Vector:
None
Properties:
acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
PSI
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
1164
1846
negative selection marker
sacB
sacB cds
5901
7319
promoter
T7
T7 promoter
5230
5249
recombination site
LoxP
LoxP sequence
5407
5440
repressor binding site
LacO
LacO sequence region
5249
5271
repressor protein gene
lacI
lacI cds
3764
4843
selectable marker
AmpR
ampicillin resistance gene
407
1066
trxn termination sequence
T7 term
T7 terminator sequence
7488
7534
