Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH237411.01X
Keyword:
None
Species:
Francisella tularensis subsp. tularensis SCHU S4
Type:
cDNA
Vector Name:
pBY011_GFP-NH3_CN
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
FtCD00037861
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3588nts
Open reading frame: 1 to 3588
ATGGATCAAGATATCAACGATTTATTATATGATACTAATGATTTGAAAAAAGAAAAAGTA
TGTGGCGGCTTTTTTGATTTTATCAAAGCTTGGTTTTATAATGACGTTGAAAGACATATG
TTTTTTGAAGATGAGATTATTGTGGCTCAGAAACTTAAAACGATGGATGTCATGGCTAAT
ACATACGCCTTAGAGAGAAAAAAATATTTTGAAAAATTTCTCAAAGATTCTCATAAACAA
AAAGGTTTTTTTACAAGAATAAAAGATAGAGATTTTGCTTTTTATAAAGATCCTACTAGG
GTTAAACTATGGAGTAGTATTACTGATACTGATGTCTATAGCTTTTTTATAAAGTCAAGC
GTTAACCAAGTGCTTGGTGGTGGTAATATAGCTCAACTGACATCATTACCAAAATTTAAG
TTTAATGCAATAGCATCAGTTAATAATGAAAACATCTCGGCAGCAGCTAATCTTGCAGCT
ACTGGAGCAAAAAGTTTTTTTGGAAGTTCATGTATAAATATTTGGTCTAATTTACCTGTA
TTTGATAGTGAAAGAGGAGATGTTAAATCATTTATTCCATTATACCTAAGAATAAGTGGT
AAAGCTTCATCGGCATTATTTAATTATAATATAGATATAAGTGCTAGCACAAATAGATTA
AAATTTACTGAATCAAAATGCATGGAATTATTAAATAACTTTAAGGGCAATGTAAATCTT
AGATTAGTGGCTGCAAAAAGCTCTAGAGAAGCAAAAAGCTTTATTTATACTCCAAAATTT
AAAGAAGATAGTAATAAGCATTTAAATGATTTAGGTTTTATTCTAAATACTCAGCAAGGT
TCATATAGATATTTAGTTCCTGAAATTAGGGATGGACTTGATCTAATTAGAGAATATACA
TACCCCGAAATTGAACAGATTTATTTTGGTAGGAGTGCACTTTCTGGTTATTCAAAAAGT
AATTATGACAAGCAAAGTTTAGGTATTGAGAAAAATGGTGAGGGTCTTATGACTCTTAGG
GTTAGTTCCAAAGATTATGAAAATCCACTAATTCAGGTCAAAACATCAGTTAATGTAACT
AGTTTTGATCTTTTATCAGGACAAATAAAAGGTAAGATTTTAGCTGATGACTTAATTGGT
TTTTCAGATGCTAAAGGTGCTATTAATAAGTTCAGAAACGAAGTTGATACAATTATTAAA
AACAATATTAGTGAAAGCTTTAAGTCTCCTTTTAAGATACCAAACTTATCTGAATTTAGT
GATATGGAGATAAGAGTGAGGTTTGGCTTATATTCTAAACAATCAGCATCCAAGTTGAGG
ACCATAAGAATCTCTATTCCTATGACTACTAAAATGTTAGATTTTAATCAAACATCTGGG
TCATATAATACAAAATCAGGTATGGGTTATTCAATGGCTCAGAGACATCAATTAAAAGAA
GCAATACCTTATAATGAAGGTCTTCTAGAAAATTCTGCTCAGCAGGATTTTGATTTGGTT
AAACCAACCGGAAATAGTACTCTTGGAGCTGTGCAAGATAATTTATCAAACATATATGAT
AAAAGTAGTACGTTTGACTCAGTAATAGGTTCTTTGAGAGAGCTTTATAAACTTAAAGGT
GAAGACCTAACAAATATTGATTCTCATGATATCCTTAATGTTTTATCACAAAACTCGTCT
TTATCAGGGTTATCTAGTAGTATCGCAGCAGATCTAAATGAGTTAAACTCTAAGTTTAAG
GATATTACGTCTATAAATAAAAAAACATCTGAAGCGATATTATCTACGAATTACCAGCAA
TATAATCCTAGTTTTAATAAAGCAAAAATATTCGATAATCCAGATTCTTTCTATGTTCAA
GATACTTTAATTAACTACTCACAAACCCTAAAAAAAGAAGAATTTAGAGATCAGATGTCA
ACAATAAATATTATTGAATTTGGTAGTGATGGTGACTTACCTATATTATTTAATCTTTGT
AAAATGATGACTAGCGAGTCAGCAAAAGATGGCGCAAAAAATTTTCTAAGTCAAAAGGAG
AATGTTAATAAGACTAAAGAGTTTTTTGATGCTCTAGAAAATACCACTTCTGTAATAAGT
CCAGAATCAAATATCAGTTATACATCTATTAATGTGCTTTGTAGCAAAAATGCTGGAGGT
GTAATTACTGAGAATAACAAAGCATCTTCTGTATCCCTAGGTTCAACTATTAGTTTAGCG
GATTTTAATAGAATGTATTCGCTTGACTCAAGAGTTTCTGTGCTTGGAGTATCTCCTTTA
GAAGAGTTATCAAGTTTTTCAGCGAAAAAAAATGAATATAAAATCACTTTTGAAGAATAT
TTTACTAAGTTAGCGATTCAAAGATATATGGAAGTTATTATGATAGATGATGAAGCTGAC
TTTATAAAATCATTTGTATTACCAATGATGGAGTGTCAAAAGAAACTATTAGATAATTTA
GTTATTCTAAATAAGGATGCTAAATGGAAAAGTAGTTCATCATTTAAGAGAGCTTTCTCA
AATAGAGGTAGTTTTAGTTTTACTGGTTGGCTTAAAGAGAGACTTCCTGGCTTTGATTTT
GAGCTTTCTCAGGAGTATGCTTATTTTACTGGCTTAGTAATAAACTTTGCCTTTGAAGAA
GATAACTCTTCTTCTGAAACTGTAATTAAACTATGTAAAATTATAAAATCTTTAGATAGG
CTTGTTCGAGGTTTTTATTTCTTACTAAGAAGGATGGTGAAAAATAGAAATACTAAAGTT
GGTTCTCATAATGTCTCTGAAAGTCACATTAATATGGTTAAGCAGATTTTAACATCCTTA
TGTGAACCATATCAGATTTTAACTCAGAAGGTAAGCGATAATGCTGAACTGTTGTCTGCT
TTAGTTAATATGTTGAGTTTTATGGATAGCCTTGTTGAAGTACAGCGGCTATGTGCTTCG
ATTTGTATTCCAACCAGTGCATTTTTCACTTTAGTGAATGAAAATAAAAATTTGTTGAAA
GATATTTGCTACACTGTCGAAAAAAGTGGTGTTACAGGAATAGTGGTTGAATCGACATTT
AGCTATTCGCCAGAACTCTTCAAAGAAAATCAACAAGATATTAAGCTTAACATTAAAAAC
ACGTCAGTCAGAGTACGAGATGGTAAAGTCAAGAGGGTTACCAGCATTGATAGGAATGCT
AATACTGAAGCTATCTTACAAAGTATTTATGATAATATAATTGCGTTTGATGAACAAGAT
TTAGAAAGACTATTTACGGGGTTTAGGATTTTAAAACAAGAAAATTCGACAAACACTAAT
AAAGCCGTTTTCAATATTGGTTTAACATATCATCCAATAACTAATAATGCTTTTGTTGAA
TTTAATAAGGATCAACAAAGTTTATTGTACTCATATAATAATTTAAGCAAAGATGCGTAT
GTTACAAAAATTAAAAGTATAGTTGATGCTAAAGTTGCTTTAAACCTTAGTTTTGAACAT
GAAGAGATTAGAAGTTCACAAGGTTTTAGTGTCTTAGCAACTGTACCAGCTAGAGGATAT
TCTATAGATGATTTAAATAGAAAGTATAGACCAATGATCTGGGTTTAG
Protein sequence predicted based on nucleotide sequence
MDQDINDLLYDTNDLKKEKVCGGFFDFIKAWFYNDVERHMFFEDEIIVAQKLKTMDVMAN TYALERKKYFEKFLKDSHKQKGFFTRIKDRDFAFYKDPTRVKLWSSITDTDVYSFFIKSS VNQVLGGGNIAQLTSLPKFKFNAIASVNNENISAAANLAATGAKSFFGSSCINIWSNLPV FDSERGDVKSFIPLYLRISGKASSALFNYNIDISASTNRLKFTESKCMELLNNFKGNVNL RLVAAKSSREAKSFIYTPKFKEDSNKHLNDLGFILNTQQGSYRYLVPEIRDGLDLIREYT YPEIEQIYFGRSALSGYSKSNYDKQSLGIEKNGEGLMTLRVSSKDYENPLIQVKTSVNVT SFDLLSGQIKGKILADDLIGFSDAKGAINKFRNEVDTIIKNNISESFKSPFKIPNLSEFS DMEIRVRFGLYSKQSASKLRTIRISIPMTTKMLDFNQTSGSYNTKSGMGYSMAQRHQLKE AIPYNEGLLENSAQQDFDLVKPTGNSTLGAVQDNLSNIYDKSSTFDSVIGSLRELYKLKG EDLTNIDSHDILNVLSQNSSLSGLSSSIAADLNELNSKFKDITSINKKTSEAILSTNYQQ YNPSFNKAKIFDNPDSFYVQDTLINYSQTLKKEEFRDQMSTINIIEFGSDGDLPILFNLC KMMTSESAKDGAKNFLSQKENVNKTKEFFDALENTTSVISPESNISYTSINVLCSKNAGG VITENNKASSVSLGSTISLADFNRMYSLDSRVSVLGVSPLEELSSFSAKKNEYKITFEEY FTKLAIQRYMEVIMIDDEADFIKSFVLPMMECQKKLLDNLVILNKDAKWKSSSSFKRAFS NRGSFSFTGWLKERLPGFDFELSQEYAYFTGLVINFAFEEDNSSSETVIKLCKIIKSLDR LVRGFYFLLRRMVKNRNTKVGSHNVSESHINMVKQILTSLCEPYQILTQKVSDNAELLSA LVNMLSFMDSLVEVQRLCASICIPTSAFFTLVNENKNLLKDICYTVEKSGVTGIVVESTF SYSPELFKENQQDIKLNIKNTSVRVRDGKVKRVTSIDRNANTEAILQSIYDNIIAFDEQD LERLFTGFRILKQENSTNTNKAVFNIGLTYHPITNNAFVEFNKDQQSLLYSYNNLSKDAY VTKIKSIVDAKVALNLSFEHEEIRSSQGFSVLATVPARGYSIDDLNRKYRPMIWV*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3588
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
URA
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pBY011_GFP-NH3_CN
Synonyms:
''
Description:
Yeast expression vector, adds N-terminal fluorescent (GFP) tag; amp resistance; recombinational cloning.
Comments:
This vector appears to be derived from pBY011-D123
Size (bp):
7920
Parent Vector:
None
Properties:
Gateway, acceptor (destination), fluorescent marker, recombinational cloning, with tag/fusion/marker, yeast expression, yeast shuttle
Author Name:
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pUC ori
origin of replication
5118
5800
bacterial origin
ColE1 ori
ColE1 origin of replication
0
0
negative selection marker
ccdB
ccdB death cassette ONLY in Non-Recombined (NO INSERT) form
6381
6590
promoter
Gal 1-10
Gal 1-10 promoter (yeast)
0
0
recombination site
attR1
att recombination site
7901
7917
recombination site
attB1
att recombination (half?) site
0
0
recombination site
attB2
att recombination (half?) site
0
0
recombination site
attR2
att recombination site
6215
6230
selectable marker
CmR
chlorampenicol resistance gene ONLY In Non-Recombined (NO INSERT) form
7028
7684
selectable marker
URA3
URA3 nutritional marker
0
0
selectable marker
AmpR
ampicillin resistance gene
4361
5020
tag
GFP
Green fluorescent protein coding region
0
0
yeast ARS
ARS1
ARS1 replication sequence (yeast)
0
0
yeast centromere
CEN4
CEN4 yeast centromeric sequence
0
0
