Gene:
Gene Symbol:
TM0743
Gene Name:
hypothetical protein
Original Clone ID:
8144
Keyword:
full-length cds
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00382421
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 654nts
Open reading frame: 51 to 704
GAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCAGTGATCTTCT
TGTTTCCATTTCACCTGTTTCTCGGGCACGTTGTAGCAGACCACGGTTTCACGAACAACG
CAAAGATAAGAGAGTACAGGGGCTGGAAGCTGATCGGGCACATGATCTGGTCGGTCTTTG
CCATCCTTGCCTTCACATTCGATGTTATCTTTCAGTCCACCACTGGTGTGGTTGTCTTCT
TGGTCTCTGTGGTGGTGCATCTCACGGGAGATCTCCTGAGAGTCTATCTTCACCGGAAGG
GAAAGAAGCGTTTGATAAATCTTCTGGAAGTCGCCCTTCTTGTGATTTTTCTTGTACTGA
ACGCCGCGGTGAAAGATCTCTTCAAAGGATCGTACCTGACAGCGGAGTTTGTTTTCTATC
TGCTTGGGATGAACGCGGTATCCGTTGCTGTGACGTACGTCTTCAGGAACTTCGTCCCGG
GAGATCCAAAGATATCCGATATCGAAGGGATCTCTGAAAGACTGGCTTTCTTTGTGTTCC
TCCTCGCTGGTAAGGATCTCTTCGCTTTCCTTTCCCTCGCCCTTGGTTTTCTATACCGGC
TTTGGAAGTTCAGAAAGCCCGATGTGAGGTGGTGGATGAGTCCAGTTTTTGGAGTTCTGA
TCTCCGTTCTCTGGAACTGGATGATGTACGGGAGGATCTTCTAATTAATTAACTTTAAGA
AGGAGATATACATACCCGGGTAAGTTTAAACGGTC
Protein sequence predicted based on nucleotide sequence
VIFLFPFHLFLGHVVADHGFTNNAKIREYRGWKLIGHMIWSVFAILAFTFDVIFQSTTGV VVFLVSVVVHLTGDLLRVYLHRKGKKRLINLLEVALLVIFLVLNAAVKDLFKGSYLTAEF VFYLLGMNAVSVAVTYVFRNFVPGDPKISDIEGISERLAFFVFLLAGKDLFAFLSLALGF LYRLWKFRKPDVRWWMSPVFGVLISVLWNWMMYGRIF*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 282613
Recommended expression in:
Not Applicable
Vector Name:
pMH2T7
Synonyms:
pMH-2-T7
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.
Comments:
None
Size (bp):
4084
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColEI origin of replication
1921
2603
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
256
285
selectable marker
AmpR
ampicillin resistance gene
1164
1823
tag
His
N-terminal 6x His tag
367
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
