Gene:
Gene Symbol:
TM0297
Gene Name:
short chain dehydrogenase/reductase oxidoreductase
Original Clone ID:
8073
Keyword:
full-length cds
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00382447
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 774nts
Open reading frame: 36 to 809
ATGGGATCTGATAAAATTCATCATCATCATCATCAGTGAATATTCTTGAAAAGCTTTTCT
CTTTGAAAAGAAAGGTAGCTCTGGTGACTGGTGGAGGACAGGGCATCGGGAAGGCCATCG
CCCAGGCGCTGGCAGCGGCGGGTGCAGCTGTTTTGATCATGGACATAAACGAAGAAACAG
CCAGAAGAACGGTCGAAGAGATAAAAGAGAAAGGTGGCGAAGCAGATTTCTATGTTGGGG
ATGTGACGAAAGAAGAAGATTGTTTTGGAGCGGTCAAAAAGGCGCTGGATAGGTGGGGGA
AACTCGACATAGGAGTCAACAACGCGGGAATAGGAGACTGGTGTGAAGCGGAGAATTATC
CGGTTGAGAAGTGGAAAAAGGTCATAGACGTGAATCTGGTTGGGGTGTTTCTTTCCGCAA
AAGCGGAGTTCCACGCTATGAAGGAAAGAAAATACGGAAAGATCATAAACATCGCGTCCA
TGTCCGGACACATCGTGAACAAACCTCAGAAGCAGACAGCTTACAACGCTTCGAAAGCGG
GTGTGATCCATCTCACCAGATCTCTGGCCGCCGAGTGGGCCCCGTACGGAATCAGGGTGA
ACAGCATAAGCCCCGGATACATCAGAACACCTCTCATAGAATCTCCAAACGTGAAAGATC
TTGTTCCCCTCTGGCTCGACATGATCCCTCTTGGAAGACTGGGAGAGGTGGACGATCTGA
TAGGAGCTGCTATCTTCCTTGCAAGTCCCGCCTCAGATTACATGACAGGGCACGATCTTG
TGATAGACGGAGGCTACACCGTCTGGTAATTAATAA
Protein sequence predicted based on nucleotide sequence
VNILEKLFSLKRKVALVTGGGQGIGKAIAQALAAAGAAVLIMDINEETARRTVEEIKEKG GEADFYVGDVTKEEDCFGAVKKALDRWGKLDIGVNNAGIGDWCEAENYPVEKWKKVIDVN LVGVFLSAKAEFHAMKERKYGKIINIASMSGHIVNKPQKQTAYNASKAGVIHLTRSLAAE WAPYGIRVNSISPGYIRTPLIESPNVKDLVPLWLDMIPLGRLGEVDDLIGAAIFLASPAS DYMTGHDLVIDGGYTVW*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 282173
Recommended expression in:
Not Applicable
Vector Name:
pMH2T7
Synonyms:
pMH-2-T7
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.
Comments:
None
Size (bp):
4084
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColEI origin of replication
1921
2603
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
256
285
selectable marker
AmpR
ampicillin resistance gene
1164
1823
tag
His
N-terminal 6x His tag
367
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
