DNASU Plasmid | TM0363 (T. maritima ) in pMH2T7 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM0363

Gene Name: fibronectin-binding protein

Original Clone ID:

8085

Keyword:

full-length cds

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH2T7

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

Linker not verified.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00382448

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1650nts

Open reading frame: 50 to 1699

ATCTGATAAAATTCATCATCATCATCATCACGTGGTCGACTCGAGCTTAATGCCCTTCGA
TGGGTTGCTTTTGCACAAAGCAGTCCGAGAGTTAAAGGAACTCGAAGGAGAACACTTGAG
GCAGATTTATCAACCAACGACGGTTGATTATTATTTTCTTTTCAGAAGTGCGACCGTTCG
GGTGTGTTTGAGACCCGATGTTTCCCATGTAGCGATCGCTGAAAAAGAAGAGGCATCGGA
GAAGATGCCCTCTTCGTTCACGATGCTTCTGAGAAAGGAACTGAAAGGTGCGAAACTTCT
AAAGGTAGAACAAATTGGGATGGACAGAACACTGCGGTTTTCCTTCGAGAAATTCGATGA
GATAGAGGGAATCGTTCAAAAAGATCTCTACATCGAAATAATGGGGATCCACTCGAACAT
GATACTGGTCAGAGATAGGAGGATCATTGACGCACACAGACGCATTGTAACGAAAAAGAG
AGAAATCCTCCCCGGCAGGGAGTTCGTGATTTTTCCCTCCGGAAAGGTTTCCATTTTTGA
ATTGAGAGAGCTTCCACCAGAGAGTGAAAAAACGATCCGAAACGTTCTCCTTTCACTTCT
CGAAGGCTTCTCTCCCGTCTCCGTGGAAGAGCTGATCCACAGAACCGGCTACGAACTGGA
TACTCCGTGGAAGGCTGTGGATCAAAGGAAGATACTCGAAGTTCTTGAGGAGATAAAAAA
GGAGCTGGAACTCCCGGGGGTGTTTGTCTACTACGAAGCTTCACACCCTGTGGAAGTTTC
TGCTTTTAGGTTTACTATGCTGAGCCTCAAAGAAAGGTACTTTGAAAAACCCTCCGAGGG
TATCAACGAATTTGTGAGATGGAAAGAAGAAAAATCCACCTTTGAAAACACCAAAAATCG
TTTTATCAAAGTGGTTGTGAATAGAATTGAAGATCTGGAGGATCTTGAAGAAAAACTTTC
GAGGGAACTTTCCGAAGCAGAGAAAGCCGAAAGATACAAAAAACTCGGCGATCTCATCGT
TCAGAACCTCTGGAGTATAAAGGGAAAATCCGGAGAGGTCGAACTCACGGACTGGGAGAC
AAACGAAAAGATCGTTGTGGATGTGGGAAAAGATCCTGCCCAGACGGCTCAGAAGTTCTA
CAATACCTACAAAAAACTGCAGAGAAAGAAAGAACAGGTAGAAAAACGGCTCGAAGAGAT
ACAGCGGGAAAAAGAGTATCTCTATCAGCTGTGGCAGACGATCGAAGACGCGGAAGATCT
GGAAACACTCGAAGAGATAGAAGAAGAAATGAGAGAATTTGGACTCCTGAAAGAGAGGAA
AACGAAAAAACAAAAATCAAAGAAAACCCGTTTCAGAGAGGTGTACTACGGCGGGTTCAA
AATTCTCATAGGAAGAAACAACAAACAGAACGATGAACTCGTGAGAACTTCCTCGAAGGA
GGACCTGTGGTTCCACGCTCACGAGATGCCGGGAGCCCACGTTGTGGTGAAGACAGAAGG
AAAGAAGGTGCCTCAGGAAGTCGTGGAATATGCGGCGAGTCTCGCGGCCGGTTACTCGAA
GGGAAAAAACTCCGGAAAAGTGCCGGTCGATTACACATTCATAAAATATGTAAGAAAACC
TAAGGGTTTCAAACCCGGTATGGTGATATACAAGAACTACAAAACCATTCTTGTTGAGCC
AAGGAGGATAGAGGGATAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGT
TTAAACGGTC

Protein sequence predicted based on nucleotide sequence

MPFDGLLLHKAVRELKELEGEHLRQIYQPTTVDYYFLFRSATVRVCLRPDVSHVAIAEKE
EASEKMPSSFTMLLRKELKGAKLLKVEQIGMDRTLRFSFEKFDEIEGIVQKDLYIEIMGI
HSNMILVRDRRIIDAHRRIVTKKREILPGREFVIFPSGKVSIFELRELPPESEKTIRNVL
LSLLEGFSPVSVEELIHRTGYELDTPWKAVDQRKILEVLEEIKKELELPGVFVYYEASHP
VEVSAFRFTMLSLKERYFEKPSEGINEFVRWKEEKSTFENTKNRFIKVVVNRIEDLEDLE
EKLSRELSEAEKAERYKKLGDLIVQNLWSIKGKSGEVELTDWETNEKIVVDVGKDPAQTA
QKFYNTYKKLQRKKEQVEKRLEEIQREKEYLYQLWQTIEDAEDLETLEEIEEEMREFGLL
KERKTKKQKSKKTRFREVYYGGFKILIGRNNKQNDELVRTSSKEDLWFHAHEMPGAHVVV
KTEGKKVPQEVVEYAASLAAGYSKGKNSGKVPVDYTFIKYVRKPKGFKPGMVIYKNYKTI
LVEPRRIEG*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Protein_Purified

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in 282238

Recommended expression in:

Not Applicable

Vector Name:

pMH2T7

Synonyms:

pMH-2-T7

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.

Comments:

None

Size (bp):

4084

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColEI origin of replication

1921

2603

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1164

1823

tag

His

N-terminal 6x His tag

367

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

Gene

Gene Symbol :

TM0363

GENEID :

897322

Locus Tag :

TM0363

GI :

15643131

GenBank Accession :

AAD35450

Target GenBank:

NP_228174

Protein

Structural Annotation Wiki :

TOPSAN

UniProt :

Q9WYJ8

Clone

Cloning Information :

276302

HIP Master Clone ID :

205788

Original Clone ID :

8085

Experimental

TargetTrack - Experimental data :

282238

Organism

Species Specific ID:

897322

Reagents

Labome :

TM0363-cDNA