Gene:
Gene Symbol:
TM1195
Gene Name:
beta-galactosidase
Original Clone ID:
8235
Keyword:
full-length cds
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00382464
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1950nts
Open reading frame: 51 to 2000
AGATATACATACCCATGTGATCTGATAAAATTCATCATCATCATCATCACATGCTCGGCG
TCTGTTACTATCCAGAACACTGGGGAACAGAGAAAGTTGAAGAAGACTTCAGAAGGATGA
AAGAACTCGGGATAGAGTACGTGAGGATTGGAGAGTTCGCCTGGAGCAGGATAGAGTCGG
AGCGTGGAAAGTTCAACTGGGACTGGCTCGACAAAACACTTGAGCTGGCAGAGAAGATGG
GGCTCAAGATCGTACTGGGAACTCCCACAGCCACACCTCCGAAGTGGCTCATCGATGAAC
ACCCGGAGATCCTTCCCGTTGATAAAGATGGCAGGGTGAAAAATTTTGGTTCCAGAAGAC
ATTATTGCTTCTCCAGCCCTGTTTATAGAGAGGAAGTGAAAAGAATCGTCACCATAATAG
TGAAAAGATACGGAAAACACCCGGCAGTCGCAGGCTGGCAGACGGACAACGAGTACGGCT
GTCACGATACGGTGAGGTGCTACTGTCCGAGGTGCAAAAAAGCCTTCCAAAAATGGCTGG
AAAGAAAGTACGAGGGAGACATAAAGAAATTGAATGAAGCGTGGGGAACAGTGTTCTGGA
GCCAGGAGTATCGATCCTTCGACGAAATAGAGCTTCCGAATCTCACCCCTGCCGACCCGA
ACCCGTCGCATCTTCTCGACTACTACAGGTTAGCCTCCGACCAAGTGGTGGAATTCAACA
AGCTCCAGGTGGAGATAATAAGGGAGTATTCGCCGGGAAGATTCATCACACACAATTTCA
TGTCCGGTTTCACAGATTTTGATCATTACAAACTCTCGAAAGACCTGGATTTCGCAACCT
GGGACAATTACCCGCTCGGACACACCCTCGTTTTTCTGAGAATGAAGGGTGAGACAAAAA
ATCCGTTCGACAGAGTGGGACACCCGGACATCATCTCATTCTCGCACGATCTGTACCGGG
GAGTGGGCAGAGGAAGATTCTGGGTGATGGAACAGCAAGCAGGACCTGTGAACTGGGCTC
CTTACAATCTCTGGCCCGCCAAGGGAGCGGTGAGACTCTGGACGTGGCAGGCGTTCGCAC
ACGGTGCGGAGGTGGTCTCCTACTTCAGATGGAGACAGGCACCTTTTGCGCAGGAGCAGA
TGCACTCTGGACTTCTGGCACCGGATTCAGCACCTTACCCTGGATACCACGAGGTAAAGC
AGGTTTTCGAGGAGCTCAAAAACATCGATATCAATGAGCCCGTGGAAAGCGAAGTGGCAC
TTGTCTTCGATTATGAAACGGCGTGGGTCTTCTCCATACAACCGCACGGCGAGGGGGTGA
ACTACATCGATCTTGTCTTCAGATTCTACAGTGCCCTCAGAAGACTCGGTCTGAACGTGG
ATATAGTACCCCCTGGATCTTCACTGGACGGATACAAAATGATCGTTGTTCCAAGCCTTG
CCATCGTGAAGGAGGAGGTTCTGGACACGTTCAAAAAATACGACGGTCTTCTCGTACTTG
GCCCAAGAAGCGGAAGCAAAACAGAGACATTCCAGATACCACCAGAAATGCCCCCAGGTC
TTCTCAAGGAGATCATACCCGTTGAAGTGAGACAGGTTGAAAGCCTTGGAGATAATGTTG
AGACACTTGTTTGGAACGGTAAGGAATACCCTGTCTCGATATGGAGAGAGGATGTGGACC
CCACCATCACGGAAGTGATCGCAAGATTCAAAGATGGTTTTGGAGCCATCTTTCGAAAGG
AAAACGTCTTCTACCTTGCTTTCTGGCCGAATGGAGACTTTCTCGTAGATTTCTTTGAAG
CACTTTCAAAAGAATCAGGAATTGAAACGAAAAGGATGCCAGAAGGAGTACGCATTCAAA
GGAGAGGTGAATATGTTTTTTCTTTCAATTTCACCTCTGAGGAGGTAGATTTAGAAATAC
CAACGAAAGTTCAGATAGTTCTAGGAGATCAAAAGATTCCTCCCTATGGACTGCTGATAT
GGAAGGAAAACGAACGCTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGA
GAAGATTTTCA
Protein sequence predicted based on nucleotide sequence
MLGVCYYPEHWGTEKVEEDFRRMKELGIEYVRIGEFAWSRIESERGKFNWDWLDKTLELA EKMGLKIVLGTPTATPPKWLIDEHPEILPVDKDGRVKNFGSRRHYCFSSPVYREEVKRIV TIIVKRYGKHPAVAGWQTDNEYGCHDTVRCYCPRCKKAFQKWLERKYEGDIKKLNEAWGT VFWSQEYRSFDEIELPNLTPADPNPSHLLDYYRLASDQVVEFNKLQVEIIREYSPGRFIT HNFMSGFTDFDHYKLSKDLDFATWDNYPLGHTLVFLRMKGETKNPFDRVGHPDIISFSHD LYRGVGRGRFWVMEQQAGPVNWAPYNLWPAKGAVRLWTWQAFAHGAEVVSYFRWRQAPFA QEQMHSGLLAPDSAPYPGYHEVKQVFEELKNIDINEPVESEVALVFDYETAWVFSIQPHG EGVNYIDLVFRFYSALRRLGLNVDIVPPGSSLDGYKMIVVPSLAIVKEEVLDTFKKYDGL LVLGPRSGSKTETFQIPPEMPPGLLKEIIPVEVRQVESLGDNVETLVWNGKEYPVSIWRE DVDPTITEVIARFKDGFGAIFRKENVFYLAFWPNGDFLVDFFEALSKESGIETKRMPEGV RIQRRGEYVFSFNFTSEEVDLEIPTKVQIVLGDQKIPPYGLLIWKENER*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 283060
Recommended expression in:
Not Applicable
Vector Name:
pMH2T7
Synonyms:
pMH-2-T7
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.
Comments:
None
Size (bp):
4084
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColEI origin of replication
1921
2603
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
256
285
selectable marker
AmpR
ampicillin resistance gene
1164
1823
tag
His
N-terminal 6x His tag
367
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
