Gene:
Gene Symbol:
hisS
Gene Name:
histidyl-tRNA synthetase
Original Clone ID:
17283
Keyword:
full-length cds
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00383225
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1263nts
Open reading frame: 9 to 1271
CGAGCTTATTGAAATACAGAAGGATAAAGGGAACAAACGATATCTTCGGTGAAGAGATAT
GGTACTGGAGGTACGTCGAGGAAACCTTCAGAAATGTGTGCGAAAGCGCGGGAATAGAGG
AGATCAGAACTCCCATATTCGAACAGACGGAACTTTTCGTTAGAAGTGTGGGAGAAGAAT
CAGACATCGTTCAGAAAGAGATGTACACCTTCCAGGACAAAGCGGGAAGGAGCATCACTC
TGAGACCAGAAGGTACCGCGCCCGTCGTCAGGGCTTTTCTGGAGAATTCTCTGATAGATA
GGGGGTTTCAACAGAGATACTACTACATAGGTCCCATGTTCCGATACGAAAAACCACAAT
CGGGGAGATTGAGACAGTTTCACCAGGTGGGTTTCGAGATCATCGGCCCCGAATCTCCAA
AAGCGGATTTCGAGGTGATCATGCTGGTTGATACCTTCTTGAGAAGGCTCGGACTCACGA
AATACAAGATTCACCTGAATTCCATAGGCTGTCCGGTGTGCAGGAAAAACTATCGCGAAG
CCCTCAAGGAGTATTACGGTCGAATCCTGGACAATCTCTGTGACGACTGCAAAAGACGTT
ACGAGACGAACATTTTGAGACTGCTCGATTGCAAGGTGGACCACGAATACTCTCTGAATG
CACCAAAAAGCGTCGACTATCTCTGTGATTCCTGCAGGGCACACTACAAGAAGTTGAAAG
AGTACCTCAACACTTTCGAGATCGAATACGTGGAAGATCACACCCTGGTCCGCGGGCTCG
ACTACTACACCAGAACGGTTTTTGAGGTGAGGCACGAAGGTCTTGGAGCCCAGAGCGCCA
TCGCGGGTGGTGGAAGGTACGATGGCCTCTTTGCGGAGCTTGGGGGCTCTTCCGTACCCG
CCCTTGGTTTTGCAGGTGGTATAGAGAGAATTATACTCGCTTTAAAAGCAGAGGGAATAG
AAATCCCGATGAAAAATGTTCATCTTGTTTACATCGCAACTCTTGGTGAAAAAGCCTTTA
TGGATGGTGTTCGGCTTGCGGGTGAATTGAGAAAGAAAGGCCTGAGTGTGGATGTGGACA
TCATGGATAGAAAGCTCTCCGGTCAGCTTAAGCACGCCAGCAGAATGGGATCGAGGTATG
CGGTCATTATTGGTGATGAAGAACTGGAAAAAGGAATCGTCATTCTCCGCGACCTCGAGA
CGGGAGACCAGGTTGAAATCGATCGGGACTTCGCCGCTGACTACATCGCTGAAAGGGTTT
CAAAAGATTAATTAA
Protein sequence predicted based on nucleotide sequence
LKYRRIKGTNDIFGEEIWYWRYVEETFRNVCESAGIEEIRTPIFEQTELFVRSVGEESDI VQKEMYTFQDKAGRSITLRPEGTAPVVRAFLENSLIDRGFQQRYYYIGPMFRYEKPQSGR LRQFHQVGFEIIGPESPKADFEVIMLVDTFLRRLGLTKYKIHLNSIGCPVCRKNYREALK EYYGRILDNLCDDCKRRYETNILRLLDCKVDHEYSLNAPKSVDYLCDSCRAHYKKLKEYL NTFEIEYVEDHTLVRGLDYYTRTVFEVRHEGLGAQSAIAGGGRYDGLFAELGGSSVPALG FAGGIERIILALKAEGIEIPMKNVHLVYIATLGEKAFMDGVRLAGELRKKGLSVDVDIMD RKLSGQLKHASRMGSRYAVIIGDEELEKGIVILRDLETGDQVEIDRDFAADYIAERVSKD *
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 282956
Recommended expression in:
Not Applicable
Vector Name:
pMH2T7
Synonyms:
pMH-2-T7
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.
Comments:
None
Size (bp):
4084
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColEI origin of replication
1921
2603
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
256
285
selectable marker
AmpR
ampicillin resistance gene
1164
1823
tag
His
N-terminal 6x His tag
367
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
