Gene:
Gene Symbol:
ribC
Gene Name:
riboflavin biosynthesis protein RibC
Original Clone ID:
723a3BNt1b1
Keyword:
None
Species:
Bacillus subtilis
Type:
cDNA
Vector Name:
pSGX2
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
BsCD00383625
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 954nts
Open reading frame: 43 to 996
GGAGATATACATATGGCTCATCATCACCATTACCACTCCCTTAAGACGATACATATTACA
CATCCTCATCATTTAATAAAAGAGGAGCAGGCAAAATCAGTGATGGCGTTAGGTTATTTT
GACGGCGTTCATCTCGGGCATCAAAAGGTAATCGGCACAGCGAAGCAAATAGCCGAAGAA
AAAGGTCTGACATTAGCTGTGATGACCTTTCATCCCCATCCTTCTCACGTGTTGGGCAGA
GATAAGGAACCAAAGGATCTGATTACGCCTCTTGAAGACAAAATAAACCAAATTGAACAA
TTAGGCACAGAAGTTCTGTATGTCGTTAAATTTAATGAAGTGTTTGCTTCTCTTTCTCCT
AAGCAGTTTATAGACCAGTATATTATCGGCCTTAATGTGCAGCACGCAGTGGCAGGCTTT
GACTTTACGTACGGCAAATACGGCAAGGGAACAATGAAGACCATGCCGGATGATTTAGAC
GGAAAAGCTGGGTGCACAATGGTAGAAAAATTAACGGAGCAGGATAAAAAAATCAGTTCT
TCGTATATCCGTACCGCGCTTCAAAACGGAGATGTTGAATTGGCGAATGTCTTGCTTGGA
CAACCTTATTTTATTAAAGGAATTGTCATTCATGGTGATAAAAGAGGGCGGACCATCGGG
TTTCCGACAGCGAATGTCGGTTTAAATAACAGCTATATCGTTCCGCCCACAGGTGTATAT
GCCGTAAAAGCGGAAGTGAACGGCGAAGTTTACAATGGCGTTTGCAATATTGGCTATAAG
CCAACGTTTTATGAAAAGCGCCCTGAACAGCCTTCCATCGAGGTCAATCTGTTTGATTTC
AATCAAGAGGTATATGGAGCCGCTATAAAAATCGAATGGTACAAACGGATTCGGAGCGAG
CGGAAATTCAATGGCATCAAAGAATTAACTGAGCAAATTGAGAAAGATAAGCAGGAAGCC
ATCCGTTATTTCAGCAATTTGCGGAAAGAAGGGTGATCCGAATTCGAGCTCCGTCG
Protein sequence predicted based on nucleotide sequence
KTIHITHPHHLIKEEQAKSVMALGYFDGVHLGHQKVIGTAKQIAEEKGLTLAVMTFHPHP SHVLGRDKEPKDLITPLEDKINQIEQLGTEVLYVVKFNEVFASLSPKQFIDQYIIGLNVQ HAVAGFDFTYGKYGKGTMKTMPDDLDGKAGCTMVEKLTEQDKKISSSYIRTALQNGDVEL ANVLLGQPYFIKGIVIHGDKRGRTIGFPTANVGLNNSYIVPPTGVYAVKAEVNGEVYNGV CNIGYKPTFYEKRPEQPSIEVNLFDFNQEVYGAAIKIEWYKRIRSERKFNGIKELTEQIE KDKQEAIRYFSNLRKEG*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-723a
Recommended expression in:
Not Applicable
Vector Name:
pSGX2
Synonyms:
pSGX-2, pSGX2_BN
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds N-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5328
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
TOPO recognition site
TOPO
TOPO cloning site
5097
5106
bacterial origin
ColE
ColE1 bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4984
5003
repressor protein gene
LacI
LacI cds
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
N-terminal 6xHis tag
5078
5095
trxn regulatory element
lac operator
lac operator
5003
5025
