Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
1803.1.51_GO.80030
Keyword:
full-length cds
Species:
Galdieria sulphuraria
Type:
cDNA
Vector Name:
pVP56K
Format:
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Center for Eukaryotic Structural Genomics
Center for Eukaryotic Structural Genomics
GsCD00386052
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 774nts
Open reading frame: 51 to 824
GTGCCGGGCTGGAAGTTCTGTTCCAGGGGCCCGAAAACCTGTACTTCCAGGCGATCGCCG
AATTGTTCACACTGGGTGCCTGTCGCCAAGTTGGAAAAAGTTGTTTTGTATTAAAGACAT
CAGAAGGATTAGTTATGTTAGATTGTGGTCTACATCCTTCGTATCAGGATGATAGAAGAT
ACCCTAACTTTAGTTTAGCTTTTTCTTATGGTCCTTTAAAAGCTGTCTTTATCACACACT
GCCATGCCGATCATGTCGGAGCTCTACCTATATTAACCGAGAGATGGGGTTACGATGGCC
CTATTTATATGTCTGAGCCTACAAGAAAACTTTCATACTACATATTGGAAGAGTGTGTAG
GTAGTTGGGGTGGCGACGATGAATGGACGGATTCCAGTCGCTCCGAGTGGTCGTATACAC
AACGAGAAGTAGAAAGTTGCTTAACAAAGGTAACGATTATGGAACCTGGACAATCTATTT
CGGTCGGTGAAAATGTTCAAGTGCATAGTTGGATGGCTGGACATGTGCTGGGTGCTTACA
TGTTTAGCATCGTCGTAGACAACCATCGAATATTGTATACAGGTGACTTTACCAGTTGTC
CTACTTTTCATTTACCTCCAGCACGGGTTGATGATATTCCCTACCCACCCGACGTTATCT
TAAGCGAAGCAACCTACGCCACCAGTTTCAAGGATGGAAGATTGAACAATCAAGTAGAGT
TCATTCAGAATGTGTTGGATTGTCTGTTGGATGGTGGCAAAGTGCTTGTACCGGTATTTG
CTATTGGTAGGGCGCAAGAGTTGTTGTTATTATTGGGCAAGTAGTAGGTTTAAACGAATT
CGAGCTCG
Protein sequence predicted based on nucleotide sequence
AIAELFTLGACRQVGKSCFVLKTSEGLVMLDCGLHPSYQDDRRYPNFSLAFSYGPLKAVF ITHCHADHVGALPILTERWGYDGPIYMSEPTRKLSYYILEECVGSWGGDDEWTDSSRSEW SYTQREVESCLTKVTIMEPGQSISVGENVQVHSWMAGHVLGAYMFSIVVDNHRILYTGDF TSCPTFHLPPARVDDIPYPPDVILSEATYATSFKDGRLNNQVEFIQNVLDCLLDGGKVLV PVFAIGRAQELLLLLGK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
ins@769,3
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in GO.80030
Recommended expression in:
Not Applicable
Vector Name:
pVP56K
Synonyms:
''
Sequencing Primer:
Forward:
MBP Forward
Reverse:
T7 Terminator
Description:
Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI).
Comments:
Cloning into this vector will remove the BarCat casstte
Size (bp):
7253
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
Publications:
PMID:
16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
5502
6184
promoter
T5
T5 promoter
123
169
protease cleavage site
TEV
TEV protease cleavage site
1413
1433
protease cleavage site
3C
3C protease cleavage site
1388
1412
repressor protein gene
LacI
LacI cds
3845
4924
selectable marker
Barnase
Barnase gene
1464
1800
selectable marker
CmR
Chlorampenicol resistance gene (CAT)
1903
2556
tag
His
8xHis tag
237
260
tag
MBP
MBP tag
267
1363
