DNASU Plasmid | TM1192 (T. maritima ) in pMH4 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM1192

Gene Name: alpha-galactosidase

Original Clone ID:

13495

Keyword:

full-length cds

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH4

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

High number of silent and/or missense mutations in CDS.

Mutation / Discrepancy:

Yes / Yes

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00396418

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1665nts

Open reading frame: 51 to 1715

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGGAAATAT
TCGGAAAGACCTTCAGAGAGGGAAGATTCGTTCTCAAAGAGAAAAACTTCACAGTTGAGT
TCGCGGTGGAGAAGATACACCTTGGCTGGAAGATCTCCGGCAGGGTGAAGGGAAGTCCGG
GAAGGCTTGAGGTTCTTCGAACGAAAGCACCGGAAAAGGTACTTGTGAACAACTGGCAGT
CCTGGGGACCGTGCAGGGTGGTCGATGCCTTTTCTTTCAAACCACCTGAAATAGATCCGA
ACTGGAGATACACCGCTTCGGTGGTGCCCGATGTACTTGAAAGGAACCTCCAGAGCGACT
ATTTCGTGGCTGAAGAAGGAAAAGTGTACGGTTTTCTGAGTTCGAAAATCGCACATCCTT
TCTTCGCTGTGGAAGATGGGGAACTTGTGGCATACCTCGAATATTTCGATGTCGAGTTCG
ACGACTTTGTTCCTCTTGAACCTCTCGTTGTACTCGAGGATCCCAACACACCCCTTCTTC
TGGAGAAATACGCGGAACTCGTCGGAATGGAAAACAACGCGAGAGTTCCAAAACACACAC
CCACTGGATGGTGCAGCTGGTACCATTACTTCCTTGATCTCACCTGGGAAGAGACCCTCA
AGAACCTGAAGCTCGCGAAGAATTTCCCGTTCGAGGTCTTCCAGATAGACGACGCCTACG
AAAAGGACATAGGTGACTGGCTCGTGACAAGAGGAGACTTTCCATCGGTGGAAGAGATGG
CAAAAGTTATAGCGGAAAACGGTTTCATCCCCGGGCATATGGACCGCCCCGGTTCAGTGT
TTCTGAAACCTCGGATGTATTTCACGGAACATCCCGGACTGGGTAGTGAAGGAAAACCGA
AGAGCCGAGATGGGCTTACAGAAACTGGAACAAAAAGATATACGCCCCTCGATCTTTCGA
AAGATGAGGTTCTGAACTGGCTTTTCGATCTCTTCTCATCTCTGAGAAAGATGGGCTACA
GGTACTTCAAGATCGACTTTCTCTTCGCGGGTGCCGTTCCAGGAGAAAGAAAAAAGAACA
TAACACCAATTCAGGCGTTCAGAAAAGGGATTGAGACGATCAGAAAAGCGGTGGGAGAAG
ATTCTTTCATCCTCGGATGCGGCTCTCCCCTTCTTCCCGCAGTGGGATGCGTCGACGGGA
TGAGGATAGGACCTGACACTGCGCCGTTCTGGGGAGAACATATAGAAGACAACGGAGCTC
CCGCTGCAAGATGGGCGCTGAGAAACGCCATAACGAGGTACTTCATGCACGACAGGTTCT
GGCTGAACGACCCCGACTGTCTGATACTGAGAGAGGAGAAAACGGATCTCACACAGAAGG
AAAAGGAGCTCTACTCGTACACGTGTGGAGTGCTCGACAACATGATCATAGAAAGCGATG
ATCTCTCGCTCGTCAGAGATCATGGAAAAAAGGTTCTGAAAGAAACGCTCGAACTCCTCG
GTGGAAGACCACGGGTTCAAAACATCATGTCGGAGGATCTGAGATACGAGATCGTCTCGT
CTGGCACTCTCTCAGGAAACGTCAAGATCGTGGTCGATCTGAACAGCAGAGAGTACCACC
TGGAAAAAGAAGGAAAGTCCTCCCTGAAAAAAAGAGTCGTCAAAAGAGAAGACGGAAGAA
ACTTCTACTTCTACGAAGAGGGTGAGAGAGAATAACGCGACTTAATTAACTCTAGATAAC
TGAGAAGGAGATATACATACCCGGGT

Protein sequence predicted based on nucleotide sequence

MEIFGKTFREGRFVLKEKNFTVEFAVEKIHLGWKISGRVKGSPGRLEVLRTKAPEKVLVN
NWQSWGPCRVVDAFSFKPPEIDPNWRYTASVVPDVLERNLQSDYFVAEEGKVYGFLSSKI
AHPFFAVEDGELVAYLEYFDVEFDDFVPLEPLVVLEDPNTPLLLEKYAELVGMENNARVP
KHTPTGWCSWYHYFLDLTWEETLKNLKLAKNFPFEVFQIDDAYEKDIGDWLVTRGDFPSV
EEMAKVIAENGFIPGHMDRPGSVFLKPRMYFTEHPGLGSEGKPKSRDGLTETGTKRYTPL
DLSKDEVLNWLFDLFSSLRKMGYRYFKIDFLFAGAVPGERKKNITPIQAFRKGIETIRKA
VGEDSFILGCGSPLLPAVGCVDGMRIGPDTAPFWGEHIEDNGAPAARWALRNAITRYFMH
DRFWLNDPDCLILREEKTDLTQKEKELYSYTCGVLDNMIIESDDLSLVRDHGKKVLKETL
ELLGGRPRVQNIMSEDLRYEIVSSGTLSGNVKIVVDLNSREYHLEKEGKSSLKKRVVKRE
DGRNFYFYEEGERE*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

V1M

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in 359818

Recommended expression in:

Not Applicable

Vector Name:

pMH4

Synonyms:

pMH-4

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enyzme (Pml I/Pac I) or PIPE cloning.

Comments:

None

Size (bp):

5489

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAACTTAATTAACTCTAGATAACTGAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1 bacterial origin of replication

3331

4013

inducible trxn activator protein gene

araC

araC cds

5463

4585

negative selection marker

ccdB

ccdB negative selection gene (may be gone in with-insert form)

1576

1785

promoter

T7 promoter

290

310

promoter

araC

araC promoter

153

124

selectable marker

CmR

chloramphenicol resistance gene (CmR)

484

1140

selectable marker

AmpR

ampicillin resistance gene

2574

3233

tag

His

N-terminal 6xHis tag

364

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

trxn termination sequence

rrnB

rrnB T2 transcription terminator

2238

2264

Gene

Gene Symbol :

TM1192

GENEID :

898292

Locus Tag :

TM1192

GI :

15643948

GenBank Accession :

CAA04514

Target GenBank:

NP_228997

Protein

PDB - Structural Annotation :

1ZY9

Structural Annotation Wiki :

TOPSAN

UniProt :

O33835

Protein Structure Viewer

2D Image
3D Image (Jmol)

FirstGlance in Jmol

PDB ID

Style

Color

Clone

Cloning Information :

454511

HIP Master Clone ID :

383997

Original Clone ID :

13495

Experimental

Structural Biology Knowledgebase :

SBKB (1ZY9)

TargetTrack - Experimental data :

359818

Organism

Species Specific ID:

898292

Reagents

Labome :

TM1192-cDNA