Gene:
Gene Symbol:
HSPB3
Gene Name:
heat shock 27kDa protein 3
Original Clone ID:
5962.1.94_GO.99033
Keyword:
full-length cds
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pEU-His-FV
Format:
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
PSI
Center for Eukaryotic Structural Genomics
HsCD00397017
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 480nts
Open reading frame: 51 to 530
ACATTTTACATTCTACAACTACAGCCATGGGACATCATCATCATCATCACGCGATCGCCG
AAAACCTGTACTTCCAGTCCGCAAAAATCATTTTGAGGCACCTCATAGAGATTCCAGTGC
GTTACCAGGAAGAGTTTGAAGCTCGAGGTCTAGAAGACTGCAGGCTGGATCATGCTTTAT
ATGCACTGCCTGGGCCAACCATCGTGGACCTGAGGAAAACCAGGGCAGCGCAGTCTCCTC
CAGTGGACTCAGCGGCAGAGACGCCACCCCGAGAAGGCAAATCCCACTTTCAGATCCTGC
TGGACGTGGTCCAGTTCCTCCCTGAAGACATCATCATTCAGACCTTCGAAGGCTGGCTAC
TGATAAAAGCACAACACGGAACCAGAATGGATGAGCACGGTTTTATCTCAAGAAGCTTCA
CCCGACAGTACAAACTACCAGATGGCGTGGAAATCAAAGATTTGTCTGCAGTCCTCTGTC
ATGATGGAATTTTGGTGGTGGAAGTAAAGGATCCAGTTGGGACTAAGTGATAGTTTAAAC
GAATTCGAGCTCGGTACCC
Protein sequence predicted based on nucleotide sequence
AIAENLYFQSAKIILRHLIEIPVRYQEEFEARGLEDCRLDHALYALPGPTIVDLRKTRAA QSPPVDSAAETPPREGKSHFQILLDVVQFLPEDIIIQTFEGWLLIKAQHGTRMDEHGFIS RSFTRQYKLPDGVEIKDLSAVLCHDGILVVEVKDPVGTK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.99033
Recommended expression in:
Not Applicable
Vector Name:
pEU-His-FV
Synonyms:
''
Sequencing Primer:
Forward:
pEU319 Forward
Reverse:
pEU Reverse
Description:
Cell-free expression vector, adds N-terminal 6xHis tag; amp resistance; restriction enzyme cloning (SgfI, PmeI).
Comments:
Cloning into this vector will remove the BarCat casstte. To avoid, the toxicity of the barnase gene, the vector pEU-HSBC (EvNO00306667) can be ordered, which substitutes sacB for barnase.
Size (bp):
4966
Parent Vector:
None
Properties:
cell-free expression, multiple cloning site, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
Publications:
PMID:
15782178
Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
2809
3491
gene fragment
Omega
TMV Omega
397
520
gene fragment
TMV-L
TMV-L
278
2238
gene fragment
BarCat-bar
BarCat cassette, including Barnase
572
908
gene fragment
BarCat-cat
BarCat cassette, including CAT
1010
1667
primer
His2 forward
His2 forward sequencing primer TCTTTCAAATACTTCTAGCTAGAGTA
424
449
promoter
SP6
SP6 promoter
373
390
recombination site
MCS
pF1K (Flexi-Vector) homology domain
1675
1803
selectable marker
AmpR
ampicillin resistance gene
3589
4248
tag
His
6xHis tag
527
544
