Gene:
Gene Symbol:
zgc:112426
Gene Name:
zgc:112426
Original Clone ID:
1683.1.55_GO.79322
Keyword:
full-length cds
Species:
Danio rerio
Type:
cDNA
Vector Name:
pVP33K
Format:
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
PSI
Center for Eukaryotic Structural Genomics
DrCD00397159
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 774nts
Open reading frame: 51 to 824
TGTTCCAGGGGCCCTGTTGCCCAGGTTGCTGTGAAAACCTGTACTTCCAGGCGATCGCCG
CAGAGTACACACATGTAAAATCCACCAAACTGGTCCTGAAAGGTTCAAACGATAAAAAGA
AGAAAAAGAACAAAGACAAGAAGAGGAAACGAGATGATGGAGAAGACAAGCTGGATATTG
TTGGAGGGTGGTGGACGGTGAAGTGCTTCGGTGAGGTCACAGGCACAGTGGCCATTGAGA
TGCACAAAAACTCCTACATTCATGCACTGGACACTGGACTCTTCACTGTAGGATCTCCAC
ATAAAGAGGATGAAGGTCCAGATCCTCCTGAACAGTTCACAGCTATAAAACTCTCAGATT
CCAGGATAGCACTGAAGTCAGGGTATGGGAAGTATTTGGGTATAAACTCAGAGGGAGTTG
TAGTTGGACGATCAGATGCCATAGGCTCCAGAGAGCAGTGGGAACCAGTGTTTCAAAATG
GTAAAATGGCCTTGCTTGCTGCAAACAGTTGTTTCATCTCGTACAGTGAGAGCGGAGACA
TCGTGGCCAAAAACAAGACAGCTGGAGAGGAGGAAATGATCAAGATCCGTTCATGCACAG
AAAGGGAGGTGAAGAGGAAAGATGATATTGCAGATGAAGACCGAGGCGATGTCAAGAACT
GCGAGCTCAATTATGTAAAGAAGTTCCAGAGTTTTCAGGACCGGAAGCTGAGGCTGAATG
AGGAAGACAGCGGTTCACTCAAAAAAGCCAGAACAGATGGAAAGTTCCATGAGGCACTGC
TGGACAGGAGGTCAAAGATGAAGGCTGACAGATACTGCAAGTGATAGGTTTAAACTCGTT
TCTCGTTC
Protein sequence predicted based on nucleotide sequence
AIAAEYTHVKSTKLVLKGSNDKKKKKNKDKKRKRDDGEDKLDIVGGWWTVKCFGEVTGTV AIEMHKNSYIHALDTGLFTVGSPHKEDEGPDPPEQFTAIKLSDSRIALKSGYGKYLGINS EGVVVGRSDAIGSREQWEPVFQNGKMALLAANSCFISYSESGDIVAKNKTAGEEEMIKIR SCTEREVKRKDDIADEDRGDVKNCELNYVKKFQSFQDRKLRLNEEDSGSLKKARTDGKFH EALLDRRSKMKADRYCK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in GO.79322
Recommended expression in:
Not Applicable
Vector Name:
pVP33K
Synonyms:
''
Sequencing Primer:
Forward:
MBP Forward
Reverse:
T7 Terminator
Description:
Bacterial (E. coli) expression, adds N-terminal 6xHis tag, MBP, 3CP cleavage, tetra-cys, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI).
Comments:
Cloning into this vector will remove the BarCat casstte
Size (bp):
7253
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
Publications:
PMID:
16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
5502
6184
promoter
T5
T5 promoter
123
169
protease cleavage site
TEV
TEV protease cleavage site
1415
1435
protease cleavage site
3C
3CP cleavage site
1373
1397
repressor protein gene
LacI
LacI cds
3842
4858
selectable marker
CmR
chloramphenicol resistance gene (CmR)
1900
2556
tag
His
6xHis tag
237
260
tag
MBP
MBP tag
267
1363
