Gene:
Gene Symbol:
HSPB3
Gene Name:
heat shock 27kDa protein 3
Original Clone ID:
5981.1.86_GO.99033
Keyword:
full-length cds
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pVP68K
Format:
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
PSI
Center for Eukaryotic Structural Genomics
HsCD00397240
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 480nts
Open reading frame: 51 to 530
CTTTAATTAACGGCGACGGTGCCGGGCTGGAAGTTCTGTTCCAGGGGCCCGCGATCGCCG
AAAACCTGTACTTCCAGTCCGCAAAAATCATTTTGAGGCACCTCATAGAGATTCCAGTGC
GTTACCAGGAAGAGTTTGAAGCTCGAGGTCTAGAAGACTGCAGGCTGGATCATGCTTTAT
ATGCACTGCCTGGGCCAACCATCGTGGACCTGAGGAAAACCAGGGCAGCGCAGTCTCCTC
CAGTGGACTCAGCGGCAGAGACGCCACCCCGAGAAGGCAAATCCCACTTTCAGATCCTGC
TGGACGTGGTCCAGTTCCTCCCTGAAGACATCATCATTCAGACCTTCGAAGGCTGGCTAC
TGATAAAAGCACAACACGGAACCAGAATGGATGAGCACGGTTTTATCTCAAGAAGCTTCA
CCCGACAGTACAAACTACCAGATGGCGTGGAAATCAAAGATTTGTCTGCAGTCCTCTGTC
ATGATGGAATTTTGGTGGTGGAAGTAAAGGATCCAGTTGGGACTAAGTGATAGTTTAAAC
GAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCT
Protein sequence predicted based on nucleotide sequence
AIAENLYFQSAKIILRHLIEIPVRYQEEFEARGLEDCRLDHALYALPGPTIVDLRKTRAA QSPPVDSAAETPPREGKSHFQILLDVVQFLPEDIIIQTFEGWLLIKAQHGTRMDEHGFIS RSFTRQYKLPDGVEIKDLSAVLCHDGILVVEVKDPVGTK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in GO.99033
Recommended expression in:
Not Applicable
Vector Name:
pVP68K
Synonyms:
''
Sequencing Primer:
Forward:
MBP Forward
Reverse:
T7 Terminator
Description:
Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette.
Comments:
Vector contains a lethal BarCat cassette that requires the strain BR610 for growth. Lethal cassette is removed during cloning.
Size (bp):
6353
Parent Vector:
None
Properties:
bacterial expression
Author Name:
University of Wisconsin
Center for Eukaryotic Structural Genomics
Center for Eukaryotic Structural Genomics
Publications:
PMID:
18988018
Title: Flexi vector cloning.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 bacterial origin of replication
4150
5377
gene fragment
Barnase
Barnase
1448
1780
primer
MBP forward
MBP forward primer site
1271
1291
primer
T7 term
T7 terminator reverse primer
2599
2616
promoter
T5
T5 promoter region
122
202
protease cleavage site
3C
3C protease cleavage site
1389
1412
repressor binding site
LacI
LacI coding region
2980
4071
selectable marker
KanR
Kanamycin resistance gene
5385
6179
selectable marker
CmR
chloramphenicol acetyltransferase gene
1885
2544
tag
His
N-terminal 8xHis tag
237
260
tag
MBP
N-terminal Maltose Binding Protein tag
267
1363
trxn termination sequence
rrnB
rrnB transcription terminator
2796
2892
