DNASU Plasmid | CV_3480 (C. violaceum ATCC 12472 )<br>In pMCSG19B (MBP and His-tagged expression vector)

Explanation of Terms

Gene:

Gene Symbol: CV_3480

Gene Name: AraC family transcriptional regulator

Original Clone ID:

APC66783.0_SG283_pMCSG19B

Keyword:

full length

Species:

Chromobacterium violaceum ATCC 12472

Type:

cDNA

Vector Name:

pMCSG19B

Format:

CLOSED

Source:

Midwest Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Jessica Bearden
PSI
Midwest Center for Structural Genomics

CvCD00398231

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 822nts

Open reading frame: 24 to 845

ACCTGTACTTCCAATCCAATGCCATGGACGGCTCGGAAAACGACTATTTCGCCGGCGACC
AGGGCCTGCCGGAGCCGGCGCCGCTGATCTTCCGCTTCCACCGCTTCACGCCGAAAAGCG
AGTTCGCCCGCCATCGCCATCGTTGGGGACAGCTGAGCCGCATCAACCTGGGCATGATGG
AGCTGCTGCTGGACGACAGGCGGGTGGTGGCCACCGCGCAATACCTGCTGTGGACGCCGC
CGGCGGAGCCGCACGCGGCCTACATCCACCAGGCGATGCATTACACCTCGATCTACGTCG
GCGACGAGGTGGCCGCGCGGCTGCCGCTGTCGACCTGTCTGCTGGAGCAGACGCCGCTGG
TGCGCGCGCTGCTGGACGACTTCGGCCAGCGCCAGATCACGACGCCGACCGATCCCTGGG
ACATCCGCCAGGCCGAGCTGCTATGCCGGCGCATCCTGCAATCCGGACGCGAGGCCAGCT
ACCTGCCCGACAGCCGGCAGCGGTTGCTGCAGCCGATACTGGACGCCATACGCCAGGACC
CGGCCGACACCACGCCGCTGAAAGCCTGGGCGGAACGGGTGTTCAGCACCGAGCGCACGC
TGGCGCGGCTGTTCCAGCGCGAGCTGGGCATGAGCTTCGGTCAATGGCGCAACCGGGCGC
GGCTGCTGGAAGCCTTGTCCGGGCTCAAGCAGGGCGACAGCGTGCAGGCCATCTCCGCTC
GGCTGGGCTACGCGACGCCATCGGCCTTCATCGCCATGTTCCAGAAACATCTGGGCGACT
CGCCGGAACGCTACCGCCGCCAACTGCTGGAAAGCGGCGGCTGGCCCGACGAATTCAGCG
CTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCG

Protein sequence predicted based on nucleotide sequence

MDGSENDYFAGDQGLPEPAPLIFRFHRFTPKSEFARHRHRWGQLSRINLGMMELLLDDRR
VVATAQYLLWTPPAEPHAAYIHQAMHYTSIYVGDEVAARLPLSTCLLEQTPLVRALLDDF
GQRQITTPTDPWDIRQAELLCRRILQSGREASYLPDSRQRLLQPILDAIRQDPADTTPLK
AWAERVFSTERTLARLFQRELGMSFGQWRNRARLLEALSGLKQGDSVQAISARLGYATPS
AFIAMFQKHLGDSPERYRRQLLESGGWPDEFSA*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

Find experimental protocols in APC66783.0

Recommended expression in:

Not Applicable

Vector Name:

pMCSG19B

Synonyms:

Sequencing Primer:

Forward: ANForward

Reverse: T7 terminator

Description:

Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV cleavage site, His tag, and TEV protease cleavage site; ampicillin resistance; ligation independent cloning (LIC).

Comments:

Similar to pMCSG19C except location of the TVMV CDS. See http://bioinformatics.anl.gov/mcsg/technologies/vectors.html for more details

Size (bp):

7468

Parent Vector:

None

Properties:

bacterial expression

Author Name:

Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

ATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCAGTGGTGGTGGTGGTGGTGCTCGAGTGCGGCCGCAAGCTTGTCGACGGAGCTCGAATTCGGATCCGTTATCCACTTCCAATATTGGATTGGAAGTACAGGTTCTCGGTACCCAGATCCACGCCAGAAGAGTGATGATGATGGTGGTGAGACTGGAAACGCACGGTTTCCGAGCCTGCTTTTTTGTACAAACTTGTGATCGAATTAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCCAGACCTTCATCAGTCAGCAGATAGTTTTCGAGGAACTCTTTTGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTCGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCATTCATGTGTTTGTTTTTAATCAGGTCAACCAGGAAGGTCAGACCCGCTTTCGCGCCAGCGTTATCCACGCCCACGTCTTTAATGTCGTACTTGCCGTTTTCATACTTGAACGCATAACCCCCGTCAGCAGCAATCAGCGGCCAGGTGAAGTACGGTTCTTGCAGGTTGAACATCAGCGCGCTCTTACCTTTCGCTTTCAGTTCTTTATCCAGCGCCGGGATCTCTTCCCAGGTTTTTGGCGGGTTCGGCAGCAGATCTTTGTTATAAATCAGCGATAACGCTTCAACAGCGATCGGGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCGGTCGTGTGCCCAGAAGATAATGTCAGGGCCATCGCCAGTTGCCGCAACCTGTGGGAATTTCTCTTCCAGTTTATCCGGATGCTCAACGGTGACTTTAATTCCGGTATCTTTCTCGAATTTCTTACCGACTTCAGCGAGACCGTTATAGCCTTTATCGCCGTTAATCCAGATTACCAGTTTACCTTCTTCGATTTTCATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTCTAGCACGCGTACCATGGGATCTTTATTAGTCCATGATGGCGGCAACAGTTTTTTTGGCCATGAAGTCATCTTCCGGCGCATCTTCAACCAGGGTAAAGGAACCCCAGCTGATTTTATCCGCGTTGAATTTCCAGTTTTTGCACCAACCATCCGCGGCATCTAGATAAGTCGCCACGAATTTTTCCGGAAATTCCACGAAGTAGTTGCTACCGTTGGTGGTATGAGTCAGGCTGTGGATGCCCAGAATGTTGCCATCAATGATGGAAACTAGTGGGCTGCCACACTGGCCATCTTTAGTGGTGATCCAGTGCTGCCAGAAAGAAGTGTCTTCTTTATGCACAATGTGAGAGGATTCAGACACCAGGCTCGAGACGCTTTTCTGCTGAAAGTTGGTGGACACCATGCACACACGATCTTTGATGGTCGGCTGACGGAATTTCAGTTTCTGCGGGAACGGCGGGAAGTCTTTAGCCATTTTGATAACGATAATGTCACGGCCTTCAACCGGTTTCATCTGCAGCTGGGTAGAGTTTTTGACTTTGAATTCACCATGCATGGTTTTGATGGTCAGTTCGCCATTGTTACGACGAAACAGATGCTGGTTGGCAATGATATACGGGCCAAAACCAATGCCAAACAGACGTTCACTATGACCATCCGAGGAGTTTTCCAGCAGGCATACGCAAGCAGAGATCGGATTAAAATCGCGCACGCCCTTCAGCAAAGCTTTAGACATATGTTACCTCCTTAAATCAATTCGGTCAGTGCGTCCTGCTGATGTGCTCAGTATCTCTATCACTGATAGGGATGTCAATCTCTATCACTGATAGGGACTCGAGGTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAAATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

pBR322 origin of replication

5432

gene fragment

TVMV cds

TVMV protease coding sequence

1881

2591

promoter

T7 promoter

1515

1531

protease cleavage site

TVMV

TVMV cleavage site

286

306

protease cleavage site

TEV

TEV protease cleavage site

223

243

recombination site

LIC

Ligation Independent Cloning (LIC) recombination site

204

235

repressor binding site

lac operator

LacO fragment

1490

1512

repressor binding site

tet operator

TetO fragment

2610

2765

repressor protein gene

LacI

LacI cds

3072

4031

selectable marker

AmpR

Ampicillin resistance

6220

7080

tag

His

N terminal His tag

268

285

tag

MBP

Maltose binding protein tag

337

1143

trxn termination sequence

T7 term

T7 termination sequence

Gene

Gene Symbol :

CV_3480

GENEID :

2547162

Locus Tag :

CV_3480

GI :

34104784

Target GenBank:

None

Protein

SMART domain :

HTH_ARAC : helix_turn_helix, arabinose operon control protein

Structural Annotation Wiki :

TOPSAN

UniProt :

Q7NSE6

Clone

Cloning Information :

576897

HIP Master Clone ID :

482496

Original Clone ID :

APC66783.0_SG283_pMCSG19B

Experimental

TargetTrack - Experimental data :

APC66783.0

Organism

Species Specific ID:

2547162

Reagents

Labome :

CV_3480-cDNA

CV_3480 (C. violaceum ATCC 12472 )In pMCSG19B (MBP and His-tagged expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Experimental

Organism

Reagents

CV_3480 (C. violaceum ATCC 12472 )
In pMCSG19B (MBP and His-tagged expression vector)