Gene:
Gene Symbol:
ZFYVE26
Gene Name:
zinc finger, FYVE domain containing 26
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069107)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399197
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAATCATCCATTTGGAAAAGAGGAAGCTGCTTCGCAGAAGCAGCTTTTTGGATTTTTC
TGCGAATGCCTGCGGAGGGGAGAATGGGAGCTGGCACAGGCATGTGTACCTCAGCTACAG
GAGGGACAAGGGGATATCCCAAAGAGGGTAGAAGACATACTTCAGGCATTGGTGGTGTGT
CCAAATCTGCTGAGATGTGGGCAGGACATCAACCCTCAAAGAGTAGCCTGGGTCTGGCTT
CTTGTACTGGAGAAATGGTTGGCCCGGGAAAAGAAGTTACTCCCAGTTGTTTTCCGGAGA
AAGCTTGAGTTTCTTTTATTGTCAGAAGACCTCCAAGGTGACATTCCAGAGAACATCCTC
GAGGAGCTGTATGAGACCTTAACACAGGGTGCAGTAGGCCACGTGCCTGACGGAAATCCA
AGGAGGGAGAGCTGGACTCCTCGTCTCAGCTCCGAAGCTGTCTCTGTGCTCTGGGATCTC
CTGAGGCAGTCTCCCCAGCCAGCACAGGCCCTGCTGGAGCTCCTGCTTGAGGAGGATGAC
GGTACTGGCCTCTGTCACTGGCCTCTGCAGAATGCACTGGTGGACCTCATTCGAAAGGCA
TTGCGGGCTTTGCAGGGCCCTGATTCGGTGCCCCCTGGGGTAGTCGATGCCATCTATGGA
GCCCTGCGGACTCTGCGTTGCCCCGCAGAACCACTTGGGGTTGAGTTGCATCTCCTGTGT
GAGGAACTACTAGAGGCCTGCAGGACCGAGGGGAGTCCCCTGCGGGAGGAGCGGCTGCTC
AGCTGCCTGCTGCACAAGGCCAGCCGGGGCCTGCTGTCCCTGTATGGCCATACCTATGCA
GAGAAGGTCACAGAAAAGCCACCGAGGGCTACAGCCTCGGGAAAAGTCTCACCGGATCAT
CTAGATCCTGAGCGGGCAATGCTAGCCCTGTTCTCCAATCCCAACCCAGCCGAGGCTTGG
AAAGTGGCCTATTTCTACTGCCTGAGCAACAACAAACACTTCCTCGAGCAGATTCTGGTA
ACAGCACTAACATTGTTGAAAGAAGAAGACTTCCCAAATCTTGGCTGCCTACTTGATAGA
GAATTCAGGCCCCTCAGTTGCCTGCTTGTACTCCTGGGCTGGACACACTGCCAGAGCCTA
GAGTCAGCCAAGAGGCTGCTCCAGACCCTGCACAGGACCCAGGGCCCAGGCTGTGATGAG
CTCCTCAGGGATGCCTGTGATGGGTTGTGGGCTCACCTGGAGGTCCTGGAGTGGTGCATA
CAGCAGAGCAGCAACCCCATACCAAAGAGAGATCTGTTGTATCATTTACACGGTGGAGAC
AGCCACTCAGTGCTCTACACTCTCCATCACCTTACAAACCTTCCAGCCCTCAGGGAGGAA
GATGTTCTCAAGCTCTTACAGAAAGTGCCAGCCAAGGACCCCCAGCAAGAGCCTGATGCA
GTTGATGCTCCAGTCCCTGAGCACCTGAGCCAGTGTCAGAACCTGACACTCTACCAGGGC
TTCTGTGCCATGAAGTATGCCATCTATGCCCTCTGTGTAAACTCACACCAGCACTCCCAG
TGCCAGGACTGCAAAGACAGCCTCTCTGAGGACCTGGCCTCAGCTACAGAGCCAGCGAAT
GACTCTCTCTCCTCCCCAGGTGCTGCAAATCTCTTCTCAACTTACCTGGCCAGGTGTCAA
CAGTATCTGTGCAGTATTCCTGACTCTCTGTGCCTGGAGCTTCTGGAAAACATCTTCTCA
TTGCTTCTCATCACCTCTGCTGATCTTCACCCAGAGCCTCACTTGCCTGAGGACTATGCT
GAGGATGATGACATTGAGGGGAAGAGCCCCTCAGGTTTGAGGTCCCCATCAGAGAGCCCT
CAGCACATAGCACATCCTGAAAGGAAGTCAGAACGGGGTTCCCTGGGAGTCCCAAAGACC
CTTGCTTATACAATGCCAAGCCATGTGAAGGCAGAGCCAAAAGACAGTTACCCAGGGCCT
CATAGGCACAGCTTTTTGGACTTAAAACACTTTACTAGTGGTATCAGTGGATTTCTGGCT
GATGAATTTGCAATAGGGGCCTTCCTCAGGCTTCTCCAAGAGCAACTGGATGAGATCAGT
AGCCGCAGCCCCCCTGAGAAGCCAAAGCAAGAAAGTCAGAGCTGCTCAGGAAGCAGAGAT
GGACTGCAGAGCCGCCTGCATCGACTTTCCAAGGTTGTCTCTGAGGCCCAGTGGAGACAC
AAGGTGGTGACAAGCAACCATCGTTCAGAGGAGCAACCTTCCCGAAGATACCAGCCTGCC
ACACGTCACCCCAGTCTCCGCCGGGGTCGTCGGACAAGAAGGAGCCAGGCAGATGGCCGA
GACAGAGGTTCAAACCCATCCCTGGAAAGTACAAGTAGTGAGCTGAGCACAAGTACGTCA
GAGGGAAGTCTGAGTGCCATGTCTGGCCGGAATGAGCTGCACAGTAGATTGCACCCCCAT
CCTCAAAGTTCACTCATCCCCATGATGTTCTCCCCACCTGAGTCACTGCTGGCATCCTGC
ATCCTTCGCGGGAACTTCGCAGAAGCCCATCAGGTGCTGTTCACGTTCAACCTGAAGTCC
TCACCCAGTTCAGGGGAACTGATGTTCATGGAGCGCTACCAGGAAGTGATCCAAGAACTG
GCCCAAGTAGAGCACAAGATTGAAAACCAGAACTCAGATGCGGGTAGCAGCACCATTCGG
AGAACTGGCAGTGGCCGCTCAACTCTACAGGCCATTGGCAGCGCTGCAGCAGCAGGAATG
GTGTTTTACTCTATCTCTGACGTGACTGACAAGCTGCTCAACACCTCTGGAGACCCCATC
CCCATGCTCCAGGAGGACTTTTGGATAAGCACGGCTCTAGTGGAGCCCACTGCTCCCCTG
AGAGAGGTTCTGGAAGACCTCAGTCCCCCTGCCATGGCTGCATTTGACCTAGCTTGCTCT
CAGTGCCAGCTCTGGAAAACCTGCAAGCAGCTTTTGGAGACAGCCGAACGGCGTTTGAAT
AGTAGCCTTGAAAGGCGGGGTCGACGGATAGACCACGTACTCCTAAATGCTGATGGCATT
CGAGGTTTTCCAGTTGTTCTTCAGCAAATCAGTAAGAGTCTCAATTATCTGCTTATGTCA
GCCAGTCAAACCAAATCAGAGAGTGTGGAAGAAAAGGGAGGAGGCCCTCCACGGTGCAGC
ATCACTGAACTGCTTCAGATGTGCTGGCCCAGCCTAAGCGAGGACTGTGTTGCCAGCCAC
ACCACCCTCTCCCAGCAGCTAGATCAGGTCCTTCAGTCACTGAGAGAGGCACTAGAGCTG
CCAGAGCCCAGGACTCCTCCACTGTCTTCCCTGGTGGAGCAGGCAGCCCAGAAAGCTCCA
GAGGCAGAGGCCCACCCTGTGCAGATCCAGACTCAGCTCCTCCAGAAGAACCTGGGCAAA
CAGACCCCATCAGGCAGCAGGCAGATGGACTACTTGGGCACCTTCTTCAGTTACTGCAGC
ACCCTTGCTGCAGTTCTCCTTCAAAGTTTGAGCTCTGAGCCTGATCATGTGGAGGTCAAG
GTAGGAAATCCCTTTGTTCTGCTGCAACAGAGCTCTTCCCAACTGGTGTCACATCTCCTG
TTTGAGAGACAAGTTCCCCCAGAGAGACTGGCAGCCCTTCTGGCCCAAGAGAATCTCAGC
CTAAGTGTGCCACAGGTCATCGTCAGCTGCTGCTGTGAGCCCCTTGCTCTTTGCTCATCC
CGGCAAAGCCAGCAGACCTCCTCCCTCCTGACTCGTCTGGGTACTCTGGCCCAGCTACAC
GCCTCTCACTGCCTGGATGACCTCCCACTTTCTACACCGAGCTCCCCGAGGACAACTGAG
AACCCTACATTGGAAAGAAAGCCCTACTCCTCCCCAAGGGACTCATCACTCCCAGCCCTC
ACCTCCTCTGCCTTGGCCTTTCTTAAGTCACGCTCAAAGCTCCTAGCTACGGTGGCCTGC
CTGGGGGCTTCCCCGAGGTTAAAGGTCAGCAAACCCAGCTTGTCATGGAAGGAACTTCGT
GGCCGCAGGGAGGTGCCTCTGGCTGCAGAGCAGGTAGCCCGGGAGTGTGAGCGCCTTCTG
GAACAATTCCCTCTGTTTGAGGCCTTCCTCCTGGCTGCCTGGGAGCCCCTGCGAGGGTCT
TTGCAGCAGGGGCAGAGTCTGGCAGTGAATCTCTGTGGTTGGGCCAGTCTTTCTACCGTT
CTCCTGGGCCTACATTCTCCCATTGCCCTAGATGTACTGAGTGAGGCTTTTGAGGAATCC
TTGGTGGCCAGAGATTGGTCCCGGGCCCTTCAGCTCACTGAAGTGTACGGGCGAGATGTG
GACGATTTGAGCAGCATAAAGGATGCAGTCCTGAGCTGTGCTGTGGCATGTGACAAAGAA
GGTTGGCAATACCTGTTTCCCGTGAAGGATGCATCTCTGAGAAGTCGGCTGGCCCTACAG
TTTGTGGACAGGTGGCCCCTGGAGTCATGCCTGGAGATTCTGGCCTACTGCATTTCAGAC
ACGGCTGTCCAAGAAGGACTAAAGTGTGAGCTACAGAGGAAGCTGGCGGAGCTGCAGGTG
TATCAGAAGATTCTGGGTTTGCAGTCTCCCCCAGTGTGGTGTGACTGGCAGACCTTGAGG
AGCTGTTGTGTTGAGGACCCATCAACTGTCATGAACATGATTCTAGAAGCACAGGAGTAT
GAACTGTGTGAAGAGTGGGGCTGCCTGTACCCCATTCCAAGAGAACATTTAATCAGCCTT
CATCAAAAGCATCTTCTCCACCTTCTAGAAAGAAGAGATCATGACAAGGCTCTGCAACTC
CTGCGAAGAATCCCTGACCCCACCATGTGCCTTGAAGTGACAGAGCAATCCCTCGACCAG
CACACTAGCTTGGCCACTTCTCACTTCTTGGCCAACTACCTCACCACCCACTTCTATGGA
CAACTGACTGCTGTCCGACACCGTGAAATCCAGGCGCTGTATGTGGGATCCAAGATTCTG
CTGACCCTGCCTGAGCAGCACCGGGCCAGCTATTCCCACTTGTCCTCTAACCCCCTGTTC
ATGCTGGAGCAGCTGCTTATGAACATGAAGGTGGATTGGGCCACTGTGGCTGTGCAGACT
CTCCAGCAGCTGCTGGTTGGACAGGAGATTGGCTTCACTATGGACGAGGTGGACTCACTG
CTTTCCAGATACGCAGAGAAAGCCCTGGACTTTCCATACCCTCAGAGGGAGAAACGATCA
GATTCTGTGATTCACCTCCAAGAAATTGTCCACCAGGCTGCAGATCCCGAGACCCTCCCT
AGATCACCATCAGCAGAGTTCTCTCCTGCTGCTCCTCCTGGTATCTCCAGTATACATTCC
CCTAGTCTAAGGGAAAGGAGTTTCCCACCAACCCAGCCCTCACAGGAATTTGTGCCCCCA
GCGACACCCCCTGCCAGGCACCAGTGGGTACCGGATGAGACTGAGAGTATCTGCATGGTC
TGCTGCAGGGAGCACTTCACCATGTTTAACAGGCGTCATCATTGTCGCCGCTGTGGCCGG
CTAGTGTGCAGCTCCTGCTCCACTAAGAAAATGGTGGTTGAAGGCTGCAGAGAGAACCCT
GCTCGTGTGTGTGATCAGTGCTATAGTTACTGCAACAAAGATGTACCAGAGGAGCCTTCA
GAAAAACCAGAAGCTCTAGACAGCTCCAAGAATGAAAGCCCTCCATACTCGTTTGTGGTG
AGAGTCCCCAAAGCAGATGAGGTGGAATGGATTTTGGATCTCAAAGAGGAGGAAAATGAG
CTGGTGCGGAGTGAATTTTACTATGAGCAGGCCCCCAGCGCCTCCTTGTGCATTGCCATC
CTGAATCTGCACCGGGACAGCATTGCCTGTGGTCACCAGCTGATTGAGCACTGCTGCAGG
CTCTCCAAGGGCCTCACCAACCCAGAGGTGGATGCCGGGCTGCTCACGGACATCATGAAG
CAGCTGCTGTTCAGCGCCAAGATGATGTTCGTCAAAGCCGGCCAGAGCCAAGACTTGGCT
CTTTGTGACAGCTACATCAGCAAGGTAGATGTGCTGAATATTTTAGTTGCTGCTGCCTAT
CGCCACGTGCCATCTTTGGATCAGATCTTGCAGCCAGCTGCAGTAACCAGGCTAAGGAAC
CAGCTTTTGGAAGCCGAGTACTACCAACTGGGCGTTGAGGTCTCCACAAAGACTGGGCTT
GATACCACCGGGGCGTGGCATGCTTGGGGCATGGCCTGCCTCAAAGCCGGGAACCTCACT
GCTGCACGGGAGAAGTTCAGTCGCTGTCTGAAGCCCCCATTTGACCTCAATCAGCTGAAT
CATGGCTCAAGGCTGGTGCAGGATGTGGTTGAGTACCTAGAGTCCACAGTGAGGCCCTTT
GTATCCTTGCAAGATGACGATTACTTTGCCACCCTGAGGGAACTGGAAGCTACCCTTCGG
ACGCAGAGCCTTTCTCTGGCAGTGATTCCTGAAGGGAAAATCATGAACAACACCTACTAC
CAGGAATGCCTCTTCTACCTGCACAACTATAGCACCAACCTGGCCATCATCAGCTTCTAC
GTGAGGCACAGCTGCCTGCGGGAAGCTCTTCTGCACCTTCTCAACAAGGAGAGTCCTCCA
GAAGTTTTTATAGAAGGCATTTTCCAACCAAGCTATAAAAGTGGGAAGCTACACACTTTG
GAGAACTTGCTAGAATCCATTGATCCAACCTTGGAGAGCTGGGGAAAGTACTTGATTGCT
GCCTGCCAACATTTACAGAAGAAGAACTACTACCACATTCTGTATGAGCTGCAGCAGTTT
ATGAAGGACCAAGTTCGGGCCGCCATGACCTGTATTCGGTTCTTCAGTCACAAAGCAAAG
TCATATACAGAACTGGGAGAGAAGCTCTCATGGCTACTTAAGGCCAAGGACCACCTGAAG
ATCTACCTCCAAGAAACATCCCGCAGCTCTGGAAGGAAGAAAACCACATTCTTCAGAAAG
AAGATGACTGCAGCTGATGTGTCAAGGCACATGAACACACTTCAGCTGCAGATGGAAGTG
ACCAGGTTCTTGCATCGGTGCGAAAGTGCTGGGACCTCTCAAATCACCACTTTGCCTCTG
CCAACCCTGTTTGGAAATAACCACATGAAAATGGATGTTGCCTGCAAGGTCATGCTGGGA
GGGAAAAATGTAGAAGATGGTTTTGGAATTGCTTTCCGTGTTCTGCAGGACTTCCAGCTG
GATGCTGCCATGACCTACTGCAGAGCTGCCCGCCAGTTGGTGGAGAAAGAGAAGTACAGT
GAGATCCAGCAACTGCTCAAATGTGTCAGTGAGTCAGGCATGGCAGCCAAAAGTGACGGG
GACACCATCCTCCTCAACTGCCTGGAAGCGTTCAAGAGAATTCCGCCCCAGGAGCTGGAG
GGCCTGATCCAGGCAATACACAATGATGACAACAAGGTTCGGGCCTACCTGATATGTTGC
AAACTGCGTTCTGCCTACTTGATTGCTGTGAAGCAAGAACACTCACGGGCCACAGCCCTT
GTCCAGCAGGTGCAGCAGGCCGCCAAGAGCAGCGGGGATGCAGTAGTGCAAGACATCTGT
GCCCAGTGGCTTCTGACAAGCCACCCCCGGGGTGCCCATGGCCCAGGCTCCAGGAAG
Protein sequence predicted based on nucleotide sequence
MNHPFGKEEAASQKQLFGFFCECLRRGEWELAQACVPQLQEGQGDIPKRVEDILQALVVC PNLLRCGQDINPQRVAWVWLLVLEKWLAREKKLLPVVFRRKLEFLLLSEDLQGDIPENIL EELYETLTQGAVGHVPDGNPRRESWTPRLSSEAVSVLWDLLRQSPQPAQALLELLLEEDD GTGLCHWPLQNALVDLIRKALRALQGPDSVPPGVVDAIYGALRTLRCPAEPLGVELHLLC EELLEACRTEGSPLREERLLSCLLHKASRGLLSLYGHTYAEKVTEKPPRATASGKVSPDH LDPERAMLALFSNPNPAEAWKVAYFYCLSNNKHFLEQILVTALTLLKEEDFPNLGCLLDR EFRPLSCLLVLLGWTHCQSLESAKRLLQTLHRTQGPGCDELLRDACDGLWAHLEVLEWCI QQSSNPIPKRDLLYHLHGGDSHSVLYTLHHLTNLPALREEDVLKLLQKVPAKDPQQEPDA VDAPVPEHLSQCQNLTLYQGFCAMKYAIYALCVNSHQHSQCQDCKDSLSEDLASATEPAN DSLSSPGAANLFSTYLARCQQYLCSIPDSLCLELLENIFSLLLITSADLHPEPHLPEDYA EDDDIEGKSPSGLRSPSESPQHIAHPERKSERGSLGVPKTLAYTMPSHVKAEPKDSYPGP HRHSFLDLKHFTSGISGFLADEFAIGAFLRLLQEQLDEISSRSPPEKPKQESQSCSGSRD GLQSRLHRLSKVVSEAQWRHKVVTSNHRSEEQPSRRYQPATRHPSLRRGRRTRRSQADGR DRGSNPSLESTSSELSTSTSEGSLSAMSGRNELHSRLHPHPQSSLIPMMFSPPESLLASC ILRGNFAEAHQVLFTFNLKSSPSSGELMFMERYQEVIQELAQVEHKIENQNSDAGSSTIR RTGSGRSTLQAIGSAAAAGMVFYSISDVTDKLLNTSGDPIPMLQEDFWISTALVEPTAPL REVLEDLSPPAMAAFDLACSQCQLWKTCKQLLETAERRLNSSLERRGRRIDHVLLNADGI RGFPVVLQQISKSLNYLLMSASQTKSESVEEKGGGPPRCSITELLQMCWPSLSEDCVASH TTLSQQLDQVLQSLREALELPEPRTPPLSSLVEQAAQKAPEAEAHPVQIQTQLLQKNLGK QTPSGSRQMDYLGTFFSYCSTLAAVLLQSLSSEPDHVEVKVGNPFVLLQQSSSQLVSHLL FERQVPPERLAALLAQENLSLSVPQVIVSCCCEPLALCSSRQSQQTSSLLTRLGTLAQLH ASHCLDDLPLSTPSSPRTTENPTLERKPYSSPRDSSLPALTSSALAFLKSRSKLLATVAC LGASPRLKVSKPSLSWKELRGRREVPLAAEQVARECERLLEQFPLFEAFLLAAWEPLRGS LQQGQSLAVNLCGWASLSTVLLGLHSPIALDVLSEAFEESLVARDWSRALQLTEVYGRDV DDLSSIKDAVLSCAVACDKEGWQYLFPVKDASLRSRLALQFVDRWPLESCLEILAYCISD TAVQEGLKCELQRKLAELQVYQKILGLQSPPVWCDWQTLRSCCVEDPSTVMNMILEAQEY ELCEEWGCLYPIPREHLISLHQKHLLHLLERRDHDKALQLLRRIPDPTMCLEVTEQSLDQ HTSLATSHFLANYLTTHFYGQLTAVRHREIQALYVGSKILLTLPEQHRASYSHLSSNPLF MLEQLLMNMKVDWATVAVQTLQQLLVGQEIGFTMDEVDSLLSRYAEKALDFPYPQREKRS DSVIHLQEIVHQAADPETLPRSPSAEFSPAAPPGISSIHSPSLRERSFPPTQPSQEFVPP ATPPARHQWVPDETESICMVCCREHFTMFNRRHHCRRCGRLVCSSCSTKKMVVEGCRENP ARVCDQCYSYCNKDVPEEPSEKPEALDSSKNESPPYSFVVRVPKADEVEWILDLKEEENE LVRSEFYYEQAPSASLCIAILNLHRDSIACGHQLIEHCCRLSKGLTNPEVDAGLLTDIMK QLLFSAKMMFVKAGQSQDLALCDSYISKVDVLNILVAAAYRHVPSLDQILQPAAVTRLRN QLLEAEYYQLGVEVSTKTGLDTTGAWHAWGMACLKAGNLTAAREKFSRCLKPPFDLNQLN HGSRLVQDVVEYLESTVRPFVSLQDDDYFATLRELEATLRTQSLSLAVIPEGKIMNNTYY QECLFYLHNYSTNLAIISFYVRHSCLREALLHLLNKESPPEVFIEGIFQPSYKSGKLHTL ENLLESIDPTLESWGKYLIAACQHLQKKNYYHILYELQQFMKDQVRAAMTCIRFFSHKAK SYTELGEKLSWLLKAKDHLKIYLQETSRSSGRKKTTFFRKKMTAADVSRHMNTLQLQMEV TRFLHRCESAGTSQITTLPLPTLFGNNHMKMDVACKVMLGGKNVEDGFGIAFRVLQDFQL DAAMTYCRAARQLVEKEKYSEIQQLLKCVSESGMAAKSDGDTILLNCLEAFKRIPPQELE GLIQAIHNDDNKVRAYLICCKLRSAYLIAVKQEHSRATALVQQVQQAAKSSGDAVVQDIC AQWLLTSHPRGAHGPGSRK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
