DNASU Plasmid | Detailed Plasmid Information

Explanation of Terms

Gene:

Gene Symbol: RASSF2

Gene Name: Ras association (RalGDS/AF-6) domain family member 2

Original Clone ID:

HR2904-21.3

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET21_NESG

Format:

FUSION

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Northeast Structural Genomics Consortium

HsCD00423033

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 978nts

Open reading frame: 31 to 1008

Reference Sequence Alignment

ATTTTGTTTACTTTAGAAGGAGATATACATATGGACTACAGCCACCAAACGTCCCTAGTC
CCATGTGGACAAGATAAATACATTTCCAAAAATGAACTTCTCTTGCATCTGAAGACCTAC
AACTTGTACTATGAAGGCCAGAATTTACAGCTCCGGCACCGGGAGGAAGAAGACGAGTTC
ATTGTGGAGGGGCTCCTGAACATCTCCTGGGGCCTGCGCCGGCCCATTCGCCTGCAGATG
CAGGATGACAACGAACGCATTCGACCCCCTCCATCCTCCTCCTCCTGGCACTCTGGCTGT
AACCTGGGGGCTCAGGGAACCACTCTGAAGCCCCTGACTGTGCCCAAAGTTCAGATCTCA
GAGGTGGATGCCCCGCCGGAGGGTGACCAGATGCCAAGCTCCACAGACTCCAGGGGCCTG
AAGCCCCTGCAGGAGGACACCCCACAGCTGATGCGCACACGCAGTGATGTTGGGGTGCGT
CGCCGTGGCAATGTGAGGACGCCTAGTGACCAGCGGCGAATCAGACGCCACCGCTTCTCC
ATCAACGGCCATTTCTACAACCATAAGACATCCGTGTTCACACCAGCCTATGGCTCTGTC
ACCAACGTCCGCATCAACAGCACCATGACCACCCCACAGGTCCTGAAGCTGCTGCTCAAC
AAATTTAAGATTGAGAATTCAGCAGAGGAGTTTGCCTTGTACGTGGTCCATACGAGTGGT
GAGAAACAGAAGCTGAAGGCCACCGATTACCCGCTGATTGCCCGAATCCTCCAGGGCCCA
TGTGAGCAGATCTCCAAAGTGTTCCTAATGGAGAAGGACCAGGTGGAGGAAGTCACCTAC
GACGTGGCCCAGTATATAAAGTTCGAGATGCCGGTACTTAAAAGCTTCATTCAGAAGCTC
CAGGAGGAAGAAGATCGGGAAGTAAAGAAGCTGATGCGCAAGTACACCGTGCTCCGGCTA
ATGATTCGACAGAGGCTGGAGGAGATAGCCGAGACCCCAGCAACAATCCTCGAGCACCAC
CACCACCACCA

Protein sequence predicted based on nucleotide sequence

MDYSHQTSLVPCGQDKYISKNELLLHLKTYNLYYEGQNLQLRHREEEDEFIVEGLLNISW
GLRRPIRLQMQDDNERIRPPPSSSSWHSGCNLGAQGTTLKPLTVPKVQISEVDAPPEGDQ
MPSSTDSRGLKPLQEDTPQLMRTRSDVGVRRRGNVRTPSDQRRIRRHRFSINGHFYNHKT
SVFTPAYGSVTNVRINSTMTTPQVLKLLLNKFKIENSAEEFALYVVHTSGEKQKLKATDY
PLIARILQGPCEQISKVFLMEKDQVEEVTYDVAQYIKFEMPVLKSFIQKLQEEEDREVKK
LMRKYTVLRLMIRQRLEEIAETPATI

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

ProteinPurified: Tested_Not_Purified

Find experimental protocols in HR2904

Recommended expression in:

Not Applicable

Vector Name:

pET21_NESG

Synonyms:

pET21, pET21-modified, pET21Cc, pET21_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds C-terminal 6xHis tag; ampicillin resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5415

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Thomas B. Acton
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

ATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCAGTGGTGGTGGTGGTGGTGCTCGAGTGCGGCCGCAAGCTTGTCGACGGAGCTCGAATTCGGATCCCCCGCCATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, BamHI, EcoRI, XhoI)

157

253

bacterial origin

ori

pBR322-type bacterial origin of replication

3180

3862

gene fragment

ROP

2580

2756

primer

T7 forward

T7 forward sequencing primer

283

298

primer

T7 reverse

T7 reverse sequencing primer

promoter

T7 promoter

280

299

repressor binding site

lacO

lacO regulatory sequence

258

280

repressor protein gene

LacI

752

1768

selectable marker

AmpR

ampicillin resistance gene

3960

4619

ssDNA origin

f1 ori

f1 origin

5081

5387

tag

His

C-terminal 6xHis tag

140

157

trxn termination sequence

T7 term

T7 termination sequence

Gene

Gene Symbol :

RASSF2

Symbol Nomenclature :

RASSF2

SYNONYM :

CENP-34; RASFADIN

Designation :

Ras association (RalGDS/AF-6) domain family 2|centromere protein 34|ras association domain-containing protein 2

Full Nomenclature :

Ras association (RalGDS/AF-6) domain family member 2

GENEID :

9770

Locus Tag :

RP4-599I11.1

HGNC :

9883

MIM :

609492

Vega :

OTTHUMG00000031790

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00423033      ATTTTGTTTACTTTAGAAGGAGATATACATATGGACTACAGCCACCAAACGTCCCTAGTC
NM_014737.2       ------------------------------ATGGACTACAGCCACCAAACGTCCCTAGTC
NM_170774.1       ------------------------------ATGGACTACAGCCACCAAACGTCCCTAGTC
                                                ******************************

HsCD00423033      CCATGTGGACAAGATAAATACATTTCCAAAAATGAACTTCTCTTGCATCTGAAGACCTAC
NM_014737.2       CCATGTGGACAAGATAAATACATTTCCAAAAATGAACTTCTCTTGCATCTGAAGACCTAC
NM_170774.1       CCATGTGGACAAGATAAATACATTTCCAAAAATGAACTTCTCTTGCATCTGAAGACCTAC
                  ************************************************************

HsCD00423033      AACTTGTACTATGAAGGCCAGAATTTACAGCTCCGGCACCGGGAGGAAGAAGACGAGTTC
NM_014737.2       AACTTGTACTATGAAGGCCAGAATTTACAGCTCCGGCACCGGGAGGAAGAAGACGAGTTC
NM_170774.1       AACTTGTACTATGAAGGCCAGAATTTACAGCTCCGGCACCGGGAGGAAGAAGACGAGTTC
                  ************************************************************

HsCD00423033      ATTGTGGAGGGGCTCCTGAACATCTCCTGGGGCCTGCGCCGGCCCATTCGCCTGCAGATG
NM_014737.2       ATTGTGGAGGGGCTCCTGAACATCTCCTGGGGCCTGCGCCGGCCCATTCGCCTGCAGATG
NM_170774.1       ATTGTGGAGGGGCTCCTGAACATCTCCTGGGGCCTGCGCCGGCCCATTCGCCTGCAGATG
                  ************************************************************

HsCD00423033      CAGGATGACAACGAACGCATTCGACCCCCTCCATCCTCCTCCTCCTGGCACTCTGGCTGT
NM_014737.2       CAGGATGACAACGAACGCATTCGACCCCCTCCATCCTCCTCCTCCTGGCACTCTGGCTGT
NM_170774.1       CAGGATGACAACGAACGCATTCGACCCCCTCCATCCTCCTCCTCCTGGCACTCTGGCTGT
                  ************************************************************

HsCD00423033      AACCTGGGGGCTCAGGGAACCACTCTGAAGCCCCTGACTGTGCCCAAAGTTCAGATCTCA
NM_014737.2       AACCTGGGGGCTCAGGGAACCACTCTGAAGCCCCTGACTGTGCCCAAAGTTCAGATCTCA
NM_170774.1       AACCTGGGGGCTCAGGGAACCACTCTGAAGCCCCTGACTGTGCCCAAAGTTCAGATCTCA
                  ************************************************************

HsCD00423033      GAGGTGGATGCCCCGCCGGAGGGTGACCAGATGCCAAGCTCCACAGACTCCAGGGGCCTG
NM_014737.2       GAGGTGGATGCCCCGCCGGAGGGTGACCAGATGCCAAGCTCCACAGACTCCAGGGGCCTG
NM_170774.1       GAGGTGGATGCCCCGCCGGAGGGTGACCAGATGCCAAGCTCCACAGACTCCAGGGGCCTG
                  ************************************************************

HsCD00423033      AAGCCCCTGCAGGAGGACACCCCACAGCTGATGCGCACACGCAGTGATGTTGGGGTGCGT
NM_014737.2       AAGCCCCTGCAGGAGGACACCCCACAGCTGATGCGCACACGCAGTGATGTTGGGGTGCGT
NM_170774.1       AAGCCCCTGCAGGAGGACACCCCACAGCTGATGCGCACACGCAGTGATGTTGGGGTGCGT
                  ************************************************************

HsCD00423033      CGCCGTGGCAATGTGAGGACGCCTAGTGACCAGCGGCGAATCAGACGCCACCGCTTCTCC
NM_014737.2       CGCCGTGGCAATGTGAGGACGCCTAGTGACCAGCGGCGAATCAGACGCCACCGCTTCTCC
NM_170774.1       CGCCGTGGCAATGTGAGGACGCCTAGTGACCAGCGGCGAATCAGACGCCACCGCTTCTCC
                  ************************************************************

HsCD00423033      ATCAACGGCCATTTCTACAACCATAAGACATCCGTGTTCACACCAGCCTATGGCTCTGTC
NM_014737.2       ATCAACGGCCATTTCTACAACCATAAGACATCCGTGTTCACACCAGCCTATGGCTCTGTC
NM_170774.1       ATCAACGGCCATTTCTACAACCATAAGACATCCGTGTTCACACCAGCCTATGGCTCTGTC
                  ************************************************************

HsCD00423033      ACCAACGTCCGCATCAACAGCACCATGACCACCCCACAGGTCCTGAAGCTGCTGCTCAAC
NM_014737.2       ACCAACGTCCGCATCAACAGCACCATGACCACCCCACAGGTCCTGAAGCTGCTGCTCAAC
NM_170774.1       ACCAACGTCCGCATCAACAGCACCATGACCACCCCACAGGTCCTGAAGCTGCTGCTCAAC
                  ************************************************************

HsCD00423033      AAATTTAAGATTGAGAATTCAGCAGAGGAGTTTGCCTTGTACGTGGTCCATACGAGTGGT
NM_014737.2       AAATTTAAGATTGAGAATTCAGCAGAGGAGTTTGCCTTGTACGTGGTCCATACGAGTGGT
NM_170774.1       AAATTTAAGATTGAGAATTCAGCAGAGGAGTTTGCCTTGTACGTGGTCCATACGAGTGGT
                  ************************************************************

HsCD00423033      GAGAAACAGAAGCTGAAGGCCACCGATTACCCGCTGATTGCCCGAATCCTCCAGGGCCCA
NM_014737.2       GAGAAACAGAAGCTGAAGGCCACCGATTACCCGCTGATTGCCCGAATCCTCCAGGGCCCA
NM_170774.1       GAGAAACAGAAGCTGAAGGCCACCGATTACCCGCTGATTGCCCGAATCCTCCAGGGCCCA
                  ************************************************************

HsCD00423033      TGTGAGCAGATCTCCAAAGTGTTCCTAATGGAGAAGGACCAGGTGGAGGAAGTCACCTAC
NM_014737.2       TGTGAGCAGATCTCCAAAGTGTTCCTAATGGAGAAGGACCAGGTGGAGGAAGTCACCTAC
NM_170774.1       TGTGAGCAGATCTCCAAAGTGTTCCTAATGGAGAAGGACCAGGTGGAGGAAGTCACCTAC
                  ************************************************************

HsCD00423033      GACGTGGCCCAGTATATAAAGTTCGAGATGCCGGTACTTAAAAGCTTCATTCAGAAGCTC
NM_014737.2       GACGTGGCCCAGTATATAAAGTTCGAGATGCCGGTACTTAAAAGCTTCATTCAGAAGCTC
NM_170774.1       GACGTGGCCCAGTATATAAAGTTCGAGATGCCGGTACTTAAAAGCTTCATTCAGAAGCTC
                  ************************************************************

HsCD00423033      CAGGAGGAAGAAGATCGGGAAGTAAAGAAGCTGATGCGCAAGTACACCGTGCTCCGGCTA
NM_014737.2       CAGGAGGAAGAAGATCGGGAAGTAAAGAAGCTGATGCGCAAGTACACCGTGCTCCGGCTA
NM_170774.1       CAGGAGGAAGAAGATCGGGAAGTAAAGAAGCTGATGCGCAAGTACACCGTGCTCCGGCTA
                  ************************************************************

HsCD00423033      ATGATTCGACAGAGGCTGGAGGAGATAGCCGAGACCCCAGCAACAATCCTCGAGCACCAC
NM_014737.2       ATGATTCGACAGAGGCTGGAGGAGATAGCCGAGACCCCAGCAACAATCTGA---------
NM_170774.1       ATGATTCGACAGAGGCTGGAGGAGATAGCCGAGACCCCAGCAACAATCTGA---------
                  ************************************************  .         

HsCD00423033      CACCACCACCA
NM_014737.2       -----------
NM_170774.1       -----------