Gene:
Gene Symbol:
STK11IP
Gene Name:
serine/threonine kinase 11 interacting protein
Original Clone ID:
FLH167104.01X
Keyword:
STOP codon removed based on NGS data
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00042937
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTTCGGCTCCGCCCCCCAGCGTCCCGTGGCCATGACGACCGCTCAGAGGGACTCCCTG
TTGTGGAAGCTCGCGGGGTTGCTGCGGGAGTCCGGGGATGTGGTCCTGTCTGGCTGTAGC
ACCCTGAGCCTGCTGACTCCCACACTGCAACAGCTGAACCACGTATTTGAGCTGCACCTG
GGGCCATGGGGCCCTGGCCAGACAGGCTTTGTGGCTCTGCCCTCCCATCCTGCCGACTCC
CCTGTTATTCTTCAGCTTCAGTTTCTCTTCGATGTGCTGCAGAAAACACTTTCACTCAAG
CTGGTCCATGTTGCTGGTCCTGGCCCCACAGGGCCCATCAAGATTTTCCCCTTCAAATCC
CTTCGGCACCTGGAGCTCCGAGGTGTTCCCCTCCACTGTCTGCATGGCCTCCGAGGCATC
TACTCCCAGCTGGAGACCCTGATTTGCAGCAGGAGCCTCCAGGCATTAGAGGAGCTCCTC
TCAGCCTGCGGCGGCGACTTCTGCTCTGCCCTCCCTTGGCTGGCTCTGCTTTCTGCCAAC
TTCAGCTACAATGCACTGACCGCCTTAGACAGCTCCCTGCGCCTCTTGTCAGCTCTGCGT
TTCTTGAACCTAAGCCACAATCAAGTCCAGGACTGTCAGGGATTCCTGATGGATTTGTGT
GAGCTCCACCATCTGGACATCTCCTATAATCGCCTGCATTTGGTGCCAAGAATGGGACCC
TCAGGGGCTGCTCTGGGGGTCCTGATACTGCGAGGCAATGAGCTTCGGAGCCTGCATGGC
CTAGAGCAGCTGAGGAATCTGCGGCACCTGGATTTGGCATACAACCTGCTGGAAGGACAC
CGGGAGCTGTCACCACTGTGGCTGCTGGCTGAGCTCCGCAAGCTCTACCTGGAGGGGAAC
CCTCTTTGGTTCCACCCTGAGCACCGAGCAGCCACTGCCCAGTACTTGTCACCCCGGGCC
AGGGATGCTGCTACTGGCTTCCTTCTCGATGGCAAGGTCTTGTCACTGACAGATTTTCAG
ACTCACACATCCTTGGGGCTCAGCCCCATGGGCCCACCTTTGCCCTGGCCAGTGGGGAGT
ACTCCTGAAACCTCAGGTGGCCCTGACCTGAGTGACAGCCTCTCCTCAGGGGGTGTTGTG
ACCCAGCCCCTGCTTCATAAGGTTAAGAGCCGAGTCCATGTGAGGCGGGCAAGCATCTCT
GAACCCAGTGATACGGACCCGGAGCCCGGAACTCTGAACCCCTCTCCGGCTGGATGGTTC
GTGCAGCAGCACCCGGAGCTGGAGCTCATGAGCAGCTTCCGGGAACGGTTCGGCCGCAAC
TGGCTGCAGTACAGGAGTCACCTGGAGCCCTCCGGAAACCCTCTGCCGGCCACCCCCACT
ACTTCTGCACCCAGTGCACCTCCAGCCAGCTCCCAGGGCCCCGACACTGCACCCAGACCT
TCACCCCCGCAGGAGGAAGCCAGAGGCCCCCAGGAGTCACCACAGAAAATGTCAGAGGAG
GTCAGGGCAGAGCCACAGGAGGAGGAAGAGGAGAAGGAGGGGAAGGAGGAGAAGGAGGAG
GGGGAGATGGTGGAACAGGGAGAAGAGGAGGCAGGAGAGGAGGAAGAAGAGGAGCAGGAC
CAGAAGGAAGTGGAAGCGGAACTCTGTCGCCCCTTGTTGGTGTGTCCCCTGGAGGGGCCT
GAGGGCATACGGGGCAGGGAATGCTTTCTCAGGGTCACTTCTGCCCACCTGTTTGAGGTG
GAACTCCAAGCAGCTCGCACCTTGGAGCGACTGGAGCTCCAGAGTCTGGAGGCAGCTGAG
ATAGAGCCGGAGGCCCAGGCCCAGAGGTCGCCCAGGCCCACGGGCTCAGATCTGCTCCCT
GGAGCCCCCATCCTCAGTCTGCGCTTCTCCTACATCTGCCCTGACCGGCAGTTGCGTCGC
TATTTGGTGCTGGAGCCTGATGCCCACGCAGCTGTCCAGGAGCTGCTTGCCGTGTTGACC
CCAGTCACCAATGTGGCTCGGGAACAGCTTGGGGAGGCCAGGGACCTCCTGCTGGGTAGA
TTCCAGTGTCTACGCTGTGGCCATGAGTTCAAGCCAGAGGAGCCCAGGATGGGATTAGAC
AGTGAGGAAGGCTGGAGGCCTCTGTTCCAAAAGACAGAATCTCCTGCTGTGTGTCCTAAC
TGTGGTAGTGACCACGTGGTTCTCCTCGCTGTGTCTCGGGGAACCCCCAACAGGGAGCGG
AAACAGGGAGAGCAGTCTCTGGCTCCTTCTCCGTTTGCCAGCCCTGTCTGCCACCCTCCT
GGCCATGGTGACCACCTTGACAGGGCCAAGAACAGCCCACCTCAGGCACCGAGCACCCGT
GACCATGGTAGTTGGAGCCTCAGTCCCCCCCCTGAGCGCTGTGGCCTCCGCTCTGTGGAC
CACCGACTCCGGCTCTTCCTGGATGTTGAGGTGTTCAGCGATGCCCAGGAGGAGTTCCAG
TGCTGCCTCAAGGTGCCAGTGGCATTGGCAGGCCACACTGGGGAGTTCATGTGCCTTGTG
GTTGTGTCTGACCGCAGGCTGTACCTGTTGAAGGTGACTGGGGAGATGCGTGAGCCTCCA
GCTAGCTGGCTGCAGCTGACCCTGGCTGTTCCCCTGCAGGATCTGAGTGGCATAGAGCTG
GGCCTGGCAGGCCAGAGCCTGCGGCTAGAGTGGGCAGCTGGGGCGGGCCGCTGTGTGCTG
CTGCCCCGAGATGCCAGGCATTGCCGGGCCTTCCTAGAGGAGCTCCTTGATGTCTTGCAG
TCTCTGCCCCCTGCCTGGAGGAACTGTGTCAGTGCCACAGAGGAGGAGGTCACCCCCCAG
CACCGGCTCTGGCCATTGCTGGAAAAAGACTCATCCTTGGAGGCTCGCCAGTTCTTCTAC
CTTCGGGCGTTCCTGGTTGAAGGCCCTTCCACCTGCCTCGTATCCCTGTTGCTGACTCCG
TCCACCCTGTTCCTGTTAGATGAGGATGCTGCAGGGTCCCCGGCAGAGCCCTCTCCTCCA
GCAGCATCTGGCGAAGCCTCTGAGAAGGTGCCTCCCTCGGGGCCGGGCCCTGCTGTGCGT
GTCAGGGAGCAGCAGCCACTCAGCAGCCTGAGCTCCGTGCTGCTCTACCGCTCAGCCCCT
GAGGACTTGCGGCTGCTCTTCTACGATGAGGTGTCCCGGCTGGAGAGCTTTTGGGCACTC
CGTGTGGTGTGTCAGGAGCAGCTGACAGCCCTGCTTGCCTGGATCCGGGAACCATGGGAG
GAGCTGTTTTCCGTCGGACTCCGGACAGTGATCCAAGAGGCGCTGGCCCTTGACCGA
Protein sequence predicted based on nucleotide sequence
MFGSAPQRPVAMTTAQRDSLLWKLAGLLRESGDVVLSGCSTLSLLTPTLQQLNHVFELHL GPWGPGQTGFVALPSHPADSPVILQLQFLFDVLQKTLSLKLVHVAGPGPTGPIKIFPFKS LRHLELRGVPLHCLHGLRGIYSQLETLICSRSLQALEELLSACGGDFCSALPWLALLSAN FSYNALTALDSSLRLLSALRFLNLSHNQVQDCQGFLMDLCELHHLDISYNRLHLVPRMGP SGAALGVLILRGNELRSLHGLEQLRNLRHLDLAYNLLEGHRELSPLWLLAELRKLYLEGN PLWFHPEHRAATAQYLSPRARDAATGFLLDGKVLSLTDFQTHTSLGLSPMGPPLPWPVGS TPETSGGPDLSDSLSSGGVVTQPLLHKVKSRVHVRRASISEPSDTDPEPGTLNPSPAGWF VQQHPELELMSSFRERFGRNWLQYRSHLEPSGNPLPATPTTSAPSAPPASSQGPDTAPRP SPPQEEARGPQESPQKMSEEVRAEPQEEEEEKEGKEEKEEGEMVEQGEEEAGEEEEEEQD QKEVEAELCRPLLVCPLEGPEGIRGRECFLRVTSAHLFEVELQAARTLERLELQSLEAAE IEPEAQAQRSPRPTGSDLLPGAPILSLRFSYICPDRQLRRYLVLEPDAHAAVQELLAVLT PVTNVAREQLGEARDLLLGRFQCLRCGHEFKPEEPRMGLDSEEGWRPLFQKTESPAVCPN CGSDHVVLLAVSRGTPNRERKQGEQSLAPSPFASPVCHPPGHGDHLDRAKNSPPQAPSTR DHGSWSLSPPPERCGLRSVDHRLRLFLDVEVFSDAQEEFQCCLKVPVALAGHTGEFMCLV VVSDRRLYLLKVTGEMREPPASWLQLTLAVPLQDLSGIELGLAGQSLRLEWAAGAGRCVL LPRDARHCRAFLEELLDVLQSLPPAWRNCVSATEEEVTPQHRLWPLLEKDSSLEARQFFY LRAFLVEGPSTCLVSLLLTPSTLFLLDEDAAGSPAEPSPPAASGEASEKVPPSGPGPAVR VREQQPLSSLSSVLLYRSAPEDLRLLFYDEVSRLESFWALRVVCQEQLTALLAWIREPWE ELFSVGLRTVIQEALALDR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3343
Start on reference sequence
44
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
