DNASU Plasmid | TM0632 (T. maritima ) in pMH2T7TEV (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM0632

Gene Name: extracellular polysaccharide biosynthesis-related protein

Original Clone ID:

24498

Keyword:

full-length cds

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH2T7TEV

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00432391

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1227nts

Open reading frame: 51 to 1277

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGTATCAAC
TGATTGTCTTCGATCTTGTGGTGCTTTCTGCACTCAACCTCCCTTTTTTTAACTGGTACG
TGGGAATACTCTTTTCGCTGTGTATGACACTTTCTTTCTTTGCCTTCAGGCTCTACGACC
TTGAAAATGTTCAAAACTACAACGAACAAATCATAAGAACAACTGTTGGCACTCTTTTTT
CTTTCATACTGATCCTTTTCTTCTATCCCCTCTTCGAAAAAGACATCACCCGATACAACT
TCTTCTACAACTTTTTGGTCTCTGTCGTTTTGATCCCTCCTCTGAACACCCTTCTTGGAA
AACTCCTCATCAAAAACCAGCCCACAAAAACGTTCCTTGTCGTGGGAAGAAAAGAAGAAA
TAGAACCCATCCTGAAAGAAATAGAAGAAAAAACGACGGGAAAGTACCGCTTTGCAGAGT
ACATAAACCCAACGGTTGAAGTTCTGAAAAGAAAACTCGTCCATTACGACGGAGTCCTTC
TTGCAGACCCAGAGTTCGAAGACGTTCTGAAGAAGGTGAACATCGAACGTGTGGAATACC
TGCCAAACCTTGTGGAAAAAACCCTGAAGAGAATCCCCATGAAAGTCCTGGAAAAGTTCA
GAGAGTACTACGAGGTTTGCTTCAGCGCTGTGAATGATGACTCACCGGCAAAAAGAATCC
TGGACATCGCTGTCTCTCTGATCGCCCTTGCTGTCTTCTCCCCCATCATGCTCGTTGTTG
GTATAATTATATACCTGGAAGATGGAAGGCCCGTTGTGTTCAGGCAAAAGAGGGTTGGGA
AAGATGGGAAAACATTCACGATGCTAAAACTCAGAAGTATGAGGAAAAAGCAGGAGAAAA
CACCGAAGTTCGCAGACCAGGAAAAAGACAGGATACTCAAGATTGGAAGAATCATAAGAC
CATTCAGAATAGACGAGGCGCTTCAGTTTGTGAACGTATTGAAAGGTGAGATGAGCGTTG
TCGGTCCAAGACCAGAGCAGGAAGAGTTCGTAAAGATCTTCGAAAAGCAGATTCCTTTCT
ACAGTTTGAGACACAGGGTAAAACCAGGTATCACCGGCTGGGCACAACTCATGTACAAAT
ATTCCTCCAATCTTGAAGAAGTGAAGAAAAAACTCAGTTACGACCTCTGGTACGTGAAGA
ACAGAAACATCTTTCTTGATCTTCGAATCATCCTGCAGACACTGGAGGCTGTGTTATGGA
GAAGAGGGGCGAAGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAA
GATTTT

Protein sequence predicted based on nucleotide sequence

MYQLIVFDLVVLSALNLPFFNWYVGILFSLCMTLSFFAFRLYDLENVQNYNEQIIRTTVG
TLFSFILILFFYPLFEKDITRYNFFYNFLVSVVLIPPLNTLLGKLLIKNQPTKTFLVVGR
KEEIEPILKEIEEKTTGKYRFAEYINPTVEVLKRKLVHYDGVLLADPEFEDVLKKVNIER
VEYLPNLVEKTLKRIPMKVLEKFREYYEVCFSAVNDDSPAKRILDIAVSLIALAVFSPIM
LVVGIIIYLEDGRPVVFRQKRVGKDGKTFTMLKLRSMRKKQEKTPKFADQEKDRILKIGR
IIRPFRIDEALQFVNVLKGEMSVVGPRPEQEEFVKIFEKQIPFYSLRHRVKPGITGWAQL
MYKYSSNLEEVKKKLSYDLWYVKNRNIFLDLRIILQTLEAVLWRRGAK*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

t1a,L1M

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Protein_Purified

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in 282504

Recommended expression in:

Not Applicable

Vector Name:

pMH2T7TEV

Synonyms:

pMH-2-T7-TEV

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; amp resistance; PIPE cloning.

Comments:

None

Size (bp):

4105

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCGCCTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColEI origin of replication

1942

2624

inducible trxn activator protein gene

araC

araC gene

3199

4074

promoter

T7 promoter

290

310

protease cleavage site

TEV

TEV protease cleavage site

382

402

selectable marker

AmpR

ampicillin resistance gene

1185

1844

tag

His

N-terminal 6xHis tag

364

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

trxn termination sequence

rrnB

rrnB T2 transcription termination

849

875

Gene

Gene Symbol :

TM0632

GENEID :

897728

Locus Tag :

TM0632

GI :

15643397

GenBank Accession :

AAD35716

Target GenBank:

NP_228441

Protein

UniProt :

Q9WZA0

Clone

Cloning Information :

289511

HIP Master Clone ID :

218997

Original Clone ID :

24498

Experimental

TargetTrack - Experimental data :

282504

Organism

Species Specific ID:

897728

Reagents

Labome :

TM0632-cDNA