Gene:
Gene Symbol:
CARD11
Gene Name:
caspase recruitment domain family, member 11
Original Clone ID:
CARD11_pDONR221_fusion
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
Brianne Petritis
Brianne Petritis
HsCD00433466
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGATGACTACATGGAGACGCTGAAGGATGAAGAGGACGCCTTGTGGGAGAATGTGGAG
TGTAACCGGCACATGCTCAGCCGCTATATCAACCCTGCCAAGCTCACGCCCTACCTGCGT
CAGTGTAAGGTCATTGATGAGCAGGATGAAGATGAAGTGCTTAATGCCCCTATGCTGCCA
TCCAAGATCAACCGAGCAGGCCGGCTGTTGGACATTCTACATACCAAGGGGCAAAGGGGC
TATGTGGTCTTCTTGGAGAGCCTAGAATTTTATTACCCAGAACTGTACAAACTGGTGACT
GGGAAAGAGCCCACTCGGAGATTCTCCACCATTGTGGTGGAGGAAGGCCACGAGGGCCTC
ACGCACTTCCTGATGAACGAGGTCATCAAGCTGCAGCAGCAGATGAAGGCCAAGGACCTG
CAACGCTGCGAGCTGCTGGCCAGGTTGCGGCAGCTGGAGGATGAGAAGAAGCAGATGACG
CTGACGCGCGTGGAGCTGCTAACCTTCCAGGAGCGGTACTACAAGATGAAGGAAGAGCGG
GACAGCTACAATGACGAGCTGGTCAAGGTGAAGGACGACAACTACAACTTAGCCATGCGC
TACGCACAGCTCAGTGAGGAGAAGAACATGGCGGTCATGAGGAGCCGAGACCTCCAACTC
GAGATCGATCAGCTAAAGCACCGGTTGAATAAGATGGAGGAGGAATGTAAGCTGGAGAGA
AATCAGTCTCTAAAACTGAAGAATGACATTGAAAATCGGCCCAAGAAGGAGCAGGTTCTG
GAACTGGAGCGGGAGAATGAAATGCTGAAGACCAAAAACCAGGAGCTGCAGTCCATCATC
CAGGCCGGGAAGCGCAGCCTGCCAGACTCAGACAAGGCCATCCTGGACATCTTGGAACAC
GACCGCAAGGAGGCCCTGGAGGACAGGCAGGAGCTGGTCAACAGGATCTACAACCTGCAG
GAGGAGGCCCGCCAGGCAGAGGAGCTGCGAGACAAGTACCTGGAGGAGAAGGAGGACCTG
GAGCTCAAGTGCTCGACCCTGGGAAAGGACTGTGAAATGTACAAGCACCGCATGAACACG
GTCATGCTGCAGCTGGAGGAGGTGGAGCGGGAGCGGGACCAGGCCTTCCACTCCCGAGAT
GAAGCTCAGACACAGTACTCGCAGTGCTTAATCGAAAAGGACAAGTACAGGAAGCAGATC
CGCGAGCTGGAGGAGAAGAACGATGAGATGAGGATCGAGATGGTGCGGCGGGAGGCCTGC
ATCGTCAACCTGGAGAGCAAGCTGCGGCGCCTCTCCAAGGACAGCAACAACCTGGACCAG
AGTCTGCCCAGGAACCTGCCAGTAACCATCATCTCTCAGGACTTTGGGGATGCCAGCCCC
AGGACCAATGGTCAAGAAGCTGACGATTCTTCCACCTCGGAGGAGTCACCTGAAGACAGC
AAGTACTTCCTGCCCTACCATCCGCCCCAGCGCAGGATGAACCTGAAGGGCATCCAGCTG
CAGAGAGCCAAATCCCCCATCAGCCTGAAGCGAACATCAGATTTTCAAGCCAAGGGGCAC
GAGGAAGAAGGCACGGATGCCAGCCCTAGCTCCTGCGGATCTCTGCCCATCACCAACTCC
TTCACCAAGATGCAGCCCCCCCGGAGCCGCAGCAGCATCATGTCAATCACCGCCGAGCCC
CCGGGAAACGACTCCATCGTCAGACGCTACAAGGAGGACGCGCCCCATCGCAGCACAGTC
GAAGAAGACAATGACAGCGGCGGGTTTGACGCCTTAGATCTGGATGATGACAGTCACGAA
CGCTACTCCTTCGGACCCTCCTCCATCCACTCCTCCTCCTCCTCCCACCAATCCGAGGGC
CTGGATGCCTACGACCTGGAGCAGGTCAACCTCATGTTCAGGAAGTTCTCTCTGGAAAGA
CCCTTCCGGCCTTCGGTCACCTCTGTGGGGCACGTGCGGGGCCCAGGGCCCTCGGTGCAG
CACACGACGCTGAATGGCGACAGCCTCACCTCCCAGCTCACCCTGCTGGGGGGCAACGCG
CGAGGGAGCTTCGTGCACTCGGTCAAGCCTGGCTCTCTGGCCGAGAAAGCCGGCCTCCGT
GAGGGCCACCAGCTGCTGCTGCTAGAAGGCTGCATCCGAGGCGAGAGGCAGAGTGTCCCG
TTGGACACATGCACCAAAGAGGAAGCCCACTGGACCATCCAGAGGTGCAGCGGCCCCGTC
ACGCTGCACTACAAGGTCAACCACGAAGGGTACCGGAAGCTGGTGAAGGACATGGAGGAC
GGCCTGATCACATCGGGGGACTCGTTCTACATCCGGCTGAACCTGAACATCTCCAGCCAG
CTGGACGCCTGCACCATGTCCCTGAAGTGTGACGATGTTGTGCACGTCCGTGACACCATG
TACCAGGACAGGCACGAGTGGCTGTGCGCGCGGGTCGACCCTTTCACAGACCATGACCTG
GATATGGGCACCATACCCAGCTACAGCCGAGCCCAGCAGCTCCTCCTGGTGAAACTGCAG
CGCCTGATGCACCGAGGCAGCCGGGAGGAGGTAGACGGCACCCACCACACCCTGCGGGCA
CTCCGGAACACCCTGCAGCCAGAAGAAGCGCTTTCAACAAGCGACCCCCGGGTCAGCCCC
CGTCTCTCGCGAGCAAGCTTCCTTTTTGGCCAGCTCCTTCAGTTCGTCAGCAGGTCCGAG
AACAAGTATAAGCGGATGAACAGCAACGAGCGGGTCCGCATCATCTCGGGGAGTCCGCTA
GGGAGCCTGGCCCGGTCCTCGCTGGACGCCACCAAGCTCTTGACTGAGAAGCAGGAAGAG
CTGGACCCTGAGAGCGAGCTGGGCAAGAACCTCAGCCTCATCCCCTACAGCCTGGTACGC
GCCTTCTACTGCGAGCGCCGCCGGCCCGTGCTCTTCACACCCACCGTGCTGGCCAAGACG
CTGGTGCAGAGGCTGCTCAACTCGGGAGGTGCCATGGAGTTCACCATCTGCAAGTCAGAT
ATCGTCACAAGAGATGAGTTCCTCAGAAGGCAGAAGACGGAGACCATCATCTACTCCCGA
GAGAAGAACCCCAACGCGTTCGAATGCATCGCCCCTGCCAACATTGAAGCTGTGGCCGCC
AAGAACAAGCACTGCCTGCTGGAGGCTGGGATCGGCTGCACAAGAGACTTGATCAAGTCC
AACATCTACCCCATCGTGCTCTTCATCCGGGTGTGTGAGAAGAACATCAAGAGGTTCAGA
AAGCTGCTGCCCCGACCTGAGACGGAGGAGGAGTTCCTGCGCGTGTGCCGGCTGAAGGAG
AAGGAGCTGGAGGCCCTGCCGTGCCTGTACGCCACGGTGGAACCTGACATGTGGGGCAGC
GTAGAGGAGCTGCTCCGCGTTGTCAAGGACAAGATCGGCGAGGAGCAGCGCAAGACCATC
TGGGTGGACGAGGACCAGCTGTTG
Protein sequence predicted based on nucleotide sequence
MDDYMETLKDEEDALWENVECNRHMLSRYINPAKLTPYLRQCKVIDEQDEDEVLNAPMLP SKINRAGRLLDILHTKGQRGYVVFLESLEFYYPELYKLVTGKEPTRRFSTIVVEEGHEGL THFLMNEVIKLQQQMKAKDLQRCELLARLRQLEDEKKQMTLTRVELLTFQERYYKMKEER DSYNDELVKVKDDNYNLAMRYAQLSEEKNMAVMRSRDLQLEIDQLKHRLNKMEEECKLER NQSLKLKNDIENRPKKEQVLELERENEMLKTKNQELQSIIQAGKRSLPDSDKAILDILEH DRKEALEDRQELVNRIYNLQEEARQAEELRDKYLEEKEDLELKCSTLGKDCEMYKHRMNT VMLQLEEVERERDQAFHSRDEAQTQYSQCLIEKDKYRKQIRELEEKNDEMRIEMVRREAC IVNLESKLRRLSKDSNNLDQSLPRNLPVTIISQDFGDASPRTNGQEADDSSTSEESPEDS KYFLPYHPPQRRMNLKGIQLQRAKSPISLKRTSDFQAKGHEEEGTDASPSSCGSLPITNS FTKMQPPRSRSSIMSITAEPPGNDSIVRRYKEDAPHRSTVEEDNDSGGFDALDLDDDSHE RYSFGPSSIHSSSSSHQSEGLDAYDLEQVNLMFRKFSLERPFRPSVTSVGHVRGPGPSVQ HTTLNGDSLTSQLTLLGGNARGSFVHSVKPGSLAEKAGLREGHQLLLLEGCIRGERQSVP LDTCTKEEAHWTIQRCSGPVTLHYKVNHEGYRKLVKDMEDGLITSGDSFYIRLNLNISSQ LDACTMSLKCDDVVHVRDTMYQDRHEWLCARVDPFTDHDLDMGTIPSYSRAQQLLLVKLQ RLMHRGSREEVDGTHHTLRALRNTLQPEEALSTSDPRVSPRLSRASFLFGQLLQFVSRSE NKYKRMNSNERVRIISGSPLGSLARSSLDATKLLTEKQEELDPESELGKNLSLIPYSLVR AFYCERRRPVLFTPTVLAKTLVQRLLNSGGAMEFTICKSDIVTRDEFLRRQKTETIIYSR EKNPNAFECIAPANIEAVAAKNKHCLLEAGIGCTRDLIKSNIYPIVLFIRVCEKNIKRFR KLLPRPETEEEFLRVCRLKEKELEALPCLYATVEPDMWGSVEELLRVVKDKIGEEQRKTI WVDEDQLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
