AP2A2 (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  AP2A2
Gene Name:  adaptor-related protein complex 2, alpha 2 subunit
Original Clone ID:
ccsbBroad304_05788
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00434310
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2818nts
Open reading frame: 1 to 2818

ATGCCGGCCGTGTCCAAGGGGGACGGGATGCGGGGCCTGGCGGTCTTCATCTCGGATATC
CGCAACTGTAAAAGTAAAGAAGCAGAAATAAAAAGGATAAACAAGGAACTGGCAAATATC
AGATCAAAATTTAAAGGTGACAAGGCTCTTGATGGCTATAGTAAAAAAAAGTACGTCTGC
AAGTTGCTCTTCATCTTTCTCCTTGGTCATGACATTGACTTTGGACACATGGAGGCTGTG
AACCTGCTGAGTTCAAACAGATACACGGAAAAGCAGATCGGCTACCTTTTCATCTCTGTG
TTGGTGAACTCAAACAGTGAGCTGATCCGCCTGATCAACAACGCCATCAAGAATGACCTG
GCCAGCCGCAACCCCACCTTCATGGGCCTGGCCCTGCACTGCATCGCCAGCGTGGGCAGC
CGGGAGATGGCCGAGGCCTTCGCCGGGGAGATCCCTAAGGTCCTCGTAGCCGGAGACACT
ATGGACAGCGTGAAGCAGAGCGCGGCCCTGTGCTTGCTGCGCCTGTACAGGACGTCCCCC
GATCTTGTCCCCATGGGCGACTGGACATCCCGAGTGGTGCACCTGCTCAATGACCAGCAC
TTGGGTGTGGTAACTGCAGCCACAAGTCTGATCACCACTTTAGCACAGAAGAACCCAGAA
GAGTTTAAAACCTCCGTGTCTCTGGCTGTCTCTAGGCTAAGCAGAATCGTGACGTCTGCA
TCCACAGATCTCCAGGATTACACTTACTATTTTGTCCCGGCTCCCTGGCTGTCTGTCAAA
CTGCTGAGACTGCTGCAGTGCTACCCACCCCCAGACCCTGCAGTGCGAGGCCGCCTGACT
GAGTGCCTGGAGACCATCCTGAACAAAGCCCAAGAACCGCCCAAGTCGAAGAAGGTCCAG
CACTCCAACGCGAAGAATGCCGTGCTCTTCGAGGCCATCAGCTTAATCATTCACCATGAC
AGTGAGCCGAACCTGCTCGTCCGTGCCTGCAACCAGTTGGGCCAGTTTCTGCAGCACCGC
GAGACCAACCTGCGCTACCTGGCCCTGGAGAGCATGTGCACGCTGGCCAGCTCTGAGTTC
TCCCATGAGGCTGTCAAGACGCACATCGAGACGGTCATCAACGCCCTGAAGACTGAGCGG
GACGTGAGCGTGCGGCAGCGGGCCGTGGACCTCCTCTACGCCATGTGCGACCGCAGCAAC
GCCCCACAGATCGTGGCCGAGATGCTGAGCTATCTGGAGACAGCTGACTACTCCATCCGA
GAAGAGATTGTGCTGAAGGTCGCCATCCTGGCTGAGAAGTACGCGGTGGACTACACCTGG
TATGTGGATACCATCTTGAACTTGATCCGAATTGCTGGTGATTACGTGAGTGAAGAGGTG
TGGTACCGAGTCATTCAGATCGTCATCAACCGGGACGACGTGCAGGGCTACGCGGCCAAG
ACTGTGTTCGAGGCTCTTCAGGCTCCCGCGTGCCACGAGAACCTGGTCAAAGTGGGCGGC
TACATCCTGGGGGAGTTTGGAAACTTGATAGCTGGAGACCCGAGATCCAGCCCGCTGATC
CAGTTCCACCTGCTGCACTCCAAGTTCCACCTGTGCAGCGTCCCCACCCGCGCGCTGCTC
CTGTCCACCTACATCAAGTTCGTGAACCTCTTCCCGGAGGTGAAGCCCACCATCCAGGAC
GTGCTGCGCAGCGACAGCCAGCTCAGGAACGCAGACGTGGAGCTGCAGCAGCGTGCTGTG
GAGTACCTGCGGCTCAGCNCCGTGGCCAGCACCGACATTCTGGCGACCGTGCTGGAGGAG
ATGCCCCCATTCCCGGAGCGGGAGTCCTCCATCTTGGCAAAGCTCAAGAAGAAGAAGGGC
CCCAGCACGGTGACAGACCTGGAGGACACCAAGCGGGACAGGAGTGTGGACGTGAACGGG
GGTCCTGAGCCTGCCCCAGCCAGTACCNGCGCCGTGTCTACGCCTTCTCCGTCGGCAGAC
CTGCTGGGTCTCGGGGCTGCCCCCCCTG-------GGGCCCCCCACCCTCCTCCGGCGGC
AGCGGGCTGCTCGTGGACGTGTTCTCAGACTCGGCCTCTGTGGTCGCGCCTCTCGCTCCT
GGCTCCGAAGACAACTTTGCCAGGTTTGTTTGTAAAAACAATGGTGTGTTGTTTGAAAAC
CAGCTGCTTCAAATTGGACTTAAGTCTGAATTTCGGCAGAATTTAGGTCGGATGTTTATC
TTTTATGGTAATAAGACCTCCACGCAGTTCCTAAACTTTACCCCAACACTAATCTGTTCA
GACGACCTTCAGCCTAACCTGAACCTGCAGACCAAGCCCGTGGACCCGACCGTGGAGGGG
GGCGCGCAGGTGCAGCAGGTGGTCAACATAGAGTGCGTGTCCGACTTCACGGAGGCGCCA
GTCCTCAACATTCAGTTCAGGTATGGGGGCACCTTCCAGAACGTGTCTGTGCAGCTGCCC
ATCACTCTCAACAAATTCTTCCAGCCGACAGAAATGGCTTCTCAGGATTTCTTTCAACGT
TGGAAGCAGTTGAGCAATCCACAGCAGGAAGTGCAGAACATCTTCAAAGCAAAGCACCCA
ATGGACACAGAAGTCACCAAAGCCAAGATCATTGGATTTGGTTCTGCACTTCTTGAAGAA
GTTGATCCTAATCCTGCGAATTTCGTGGGAGCTGGAATCATCCACACGAAAACCACCCAG
ATTGGATGCCTGCTGCGCTTGGAGCCGAACCTGCAAGCCCAGATGTACCGGCTCACGCTG
CGCACAAGTAAGGAAGCCGTTTCTCAGAGATTATGTGAATTGCTCTCAGCGCAGTTT

Protein sequence predicted based on nucleotide sequence

MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVC
KLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDL
ASRNPTFMGLALHCIASVGSREMAEAFAGEIPKVLVAGDTMDSVKQSAALCLLRLYRTSP
DLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSA
STDLQDYTYYFVPAPWLSVKLLRLLQCYPPPDPAVRGRLTECLETILNKAQEPPKSKKVQ
HSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEF
SHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAPQIVAEMLSYLETADYSIR
EEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAK
TVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFHLLHSKFHLCSVPTRALL
LSTYIKFVNLFPEVKPTIQDVLRSDSQLRNADVELQQRAVEYLRLSVASTDILATVLEEM
PPFPERESSILAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTAVSTPSPSADLL
GLGAAPPXXXGPPPSSGGSGLLVDVFSDSASVVAPLAPGSEDNFARFVCKNNGVLFENQL
LQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICSDDLQPNLNLQTKPVDPTVEGGA
QVQQVVNIECVSDFTEAPVLNIQFRYGGTFQNVSVQLPITLNKFFQPTEMASQDFFQRWK
QLSNPQQEVQNIFKAKHPMDTEVTKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIG
CLLRLEPNLQAQMYRLTLRTSKEAVSQRLCELLSAQF

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
>4 errors; >4 non-synonomous errors;
 Clone mixture?
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable