Gene:
Gene Symbol:
KIF14
Gene Name:
kinesin family member 14
Original Clone ID:
ccsbBroad304_07511
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00436981
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTCATTACACAGTACTCATAATAGAAATAACAGCGGTGATATTCTTGATATTCCTTCT
TCCCAAAATAGTTCATCACTGAATGCCCTCACCCACAGTAGCCGACTTAAGCTGCATTTG
AAGTCGGATATGTCAGAATGTGAAAATGATGATCCATTATTGAGATCTGCAGGTAAAGTC
AGAGACATAAATAGAACTTATGTTATTTCTGCCAGTAGAAAAACAGCAGACATGCCCCTT
ACCCCTAATCCTGTAGGTAGATTGGCACTTCAGAGGAGAACTACAAGGAACAAAGAATCA
TCTTTGCTTGTTAGTGAGTTGGAAGACACAACTGAAAAAACAGCAGAAACACGTCTTACA
TTACAACGTCGTGCTAAAACAGATTCTGCAGAAAAGTGGAAAACAGCTGAAATAGATTCT
GTCAAAATGACACTGAATGTGGGAGGTGAAACAGAAAATAATGGTGTTTCTAAGGAAAGT
AGAACAAATGTAAGGATTGTAAATAATGCTAAAAACTCTTTTGTTGCCTCTTCTGTACCT
TTAGATGAAGATCCACAGGTCATTGAAATGATGGCTGATAAGAAATACAAAGAAACATTT
TCTGCCCCCAGTAGAGCAAATGAAAATGTTGCACTTAAGTACTCAAGTAATAGACCACCC
ATTGCTTCCCTGAGTCAGACTGAAGTTGTTAGATCAGGACACTTGACAACGAAACCTACT
CAGAGCAAGTTGGATATCAAAGTGTTGGGAACAGGAAACTTGTATCATAGAAGTATTGGG
AAGGAAATTGCAAAAACTTCAAATAAATTTGGGAGCTTAGAAAAAAGAACACCTACAAAA
TGTACAACAGAACACAAACTGACAACAAAGTGCAGCCTGCCTCAGCTTAAGAGCCCAGCT
CCATCAATACTGAAGAATAGAATGTCTAACCTTCAAGTTAAACAAAGACCAAAAAGTTCC
TTTCTTGCAAATAAACAGGAAAGATCCGCAGAAAATACAATTCTTCCCGAAGAAGAAACT
GTAGTTCAGAACACCTCTGCAGGAAAAGACCCCTTAAAAGTAGAGAATAGTCAAGTGACA
GTGGCAGTACGCGTAAGACCTTTCACCAAGAGAGAGAAGATTGAAAAAGCATCCCAGGTA
GTCTTCATGAGTGGGAAAGAAATAACTGTGGAACACCCTGACACGAAACAAGTTTATAAT
TTTATTTATGATGTTTCATTCTGGTCTTTTGATGAATGTCATCCTCACTACGCTAGCCAG
ACAACTGTCTATGAGAAGCTAGCAGCACCACTCCTAGAAAGAGCCTTCGAAGGCTTCAAT
ACCTGTCTTTTTGCTTATGGTCAGACTGGCTCTGGAAAATCATATACGATGATGGGATTT
AGTGAAGAACCAGGAATAATTCCAAGATTTTGTGAAGATCTTTTTTCTCAAGTAGCCAGA
AAACAAACCCAAGAGGTCAGCTATCACATTGAAATGAGCTTCTTTGAAGTATATAATGAA
AAAATTCACGACCTTCTGGTTTGTAAAGATGAAAATGGGCAGAGAAAGCAACCACTGAGA
GTGAGGGAACATCCTGTTTATGGACCATATGTTGAAGCACTGTCAATGAACATTGTCAGT
TCTTACGCTGATATCCAGAGTTGGCTAGAATTGGGAAATAAACAAAGAGCTACTGCTGCT
ACTGGTATGAATGATAAAAGTTCCCGATCTCATTCAGTTTTCACCCTGGTGATGACCCAG
ACCAAGACAGAATTTGTGGAAGGGGAAGAACACGATCACAGAATAACAAGTCGAATTAAC
CTAATAGATCTGGCAGGCAGTGAGCGCTGCTCTACGGCTCACACTAATGGAGATCGACTA
AAGGAAGGTGTGAGTATTAATAAGTCCTTGCTAACTTTGGGAAAAGTTATATCTGCACTT
TCGGAACAAGCAAACCAAAGGAGTGTTTTTATTCCTTATCGTGAATCTGTTCTTACATGG
CTGTTAAAAGAAAGTCTGGGTGGAAATTCAAAAACTGCAATGATTGCTACGATTAGTCCC
GCTGCCAGCAACATAGAAGAAACATTAAGCACACTTAGATATGCTAACCAAGCCCGTTTA
ATAGTCAACATTGCTAAAGTAAATGAAGATATGAACGCTAAGTTAATTAGAGAATTGAAG
GCAGAAATTGCAAAGCTAAAAGCTGCTCAGAGAAACAGTCGGAATATTGACCCTGAACGA
TACAGGCTCTGTCGGCAAGAAATAACATCCTTAAGAATGAAACTGCATCAACAGGAGAGA
GACATGGCAGAAATGCAAAGAGTGTGGAAAGAAAAGTTTGAACAAGCTGAAAAAAGAAAA
CTTCAAGAAACAAAAGAGTTACAGAAAGCAGGAATTATGTTTCAAATGGACAATCATTTA
CCAAACCTTGTTAATCTGAATGAAGATCCACAACTATCTGAGATGCTGCTATATATGATA
AAAGAAGGAACAACTACAGTTGGAAAGTATAAACCAAACTCAAGCCATGATATTCAGTTA
TCTGGGGTGCTGATTGCTGATGATCATTGTACTATCAAAAATTTTGGTGGGACAGTGAGT
ATTATCCCAGTTGGGGAAGCAAAGACATATGTAAATGGAAAACATATTTTGGAAATCACA
GTATTACGTCATGGTGATCGAGTGATTCTTGGTGGAGATCATTATTTTAGATTTAATCAT
CCAGTAGAAGTCCAGAAAGGAAAAAGGCCATCTGGAAGAGATACTCCTATAAGTGAGGGT
CCAAAAGACTTTGAATTTGCAAAAAATGAGTTGCTCATGGCACAGAGATCACAACTTGAA
GCAGAAATAAAAGAGGCTCAGTTGAAGGCAAAGGAAGAAATGATGCAAGGAATCCAGATT
GCAAAAGAAATGGCTCAGCAAGAGCTTTCTTCTCAAAAAGCTGCATATGAAAGCAAAATA
AAAGCACTGGAAGCAGAACTGAGAGAAGAGTCTCAAAGGAAAAAAATGCAGGAAATAAAT
AACCAGAAGGCTAATCACAAAATTGAGGAATTAGAAAAGGCAAAGCAGCATCTTGAACAG
GAAATATATGTCAACAAAAAGCGATTAGAAATGGAGACATTGGCTACAAAACAGGCTTTA
GAAGACCATAGCATCCGCCATGCAAGAATTCTGGAAGCTTTAGAAACTGAAAAGCAAAAA
ATTGCTAAAGAAGTACAAATTCTACAGCAGAATCGGAATAATAGGGATAAAACTTTTACA
GTGCAGACAACTTGGAGCTCTATGAAACTCTCAATGATGATTCAGGAAGCCAATGCTATC
AGCAGCAAATTGAAAACATACTATGTTTTTGGCAGACATGATATATCAGATAAAAGTAGT
TCTGACACTTCTATTCGGGTTCGTAACCTGAAACTAGGAATCTCAACATTCTGGAGTCTG
GAAAAGTTTGAATCTAAACTTGCAGCAATGAAAGAACTTTATGAGAGTAATGGTAGTAAC
AGGGGTGAAGATGCCTTTTGTGATCCTGAAGATGAATGGGAACCCGACATTACAGATGCA
CCAGTTTCTTCACTTTCTAGAAGGAGGAGTAGGAGTTTGATGAAGAACAGAAGAATTTCT
GGTTGTTTACATGACATACAAGTCCATCCAATTAAGAATTTGCATTCTTCACATTCATCA
GGTTTAATGGACAAATCAAGCACTATTTACTCAAATTCAGCAGAGTCCTTTCTTCCTGGA
ATTTGCAAAGAATTGATTGGTTCTTCGTTAGATTTTTTTGGACAGAGTTATGATGAAGAA
AGAACTATAGCAGACAGCCTAATTAATAGTTTTCTTAAAATTTATAATGGGCTATTTGCC
ATTTCCAAGGCTCATGAAGAACAAGATGAAGAAAGTCAAGATAACTTGTTTTCTTCTGAT
CGAGCAATCCAGTCACTTACTATTCAGACTGCATGTGCTTTTGAGCAGCTAGTAGTGCTA
ATGAAACACTGGCTGAGTGATTTACTGCCTTGTACCAACATAGCAAGACTTGAGGATGAA
TTGAGACAAGAAGTTAAAAAACTGGGAGGCTACTTACAGTTATTTTTGCAGGGATGCTGT
TTGGATATTTCATCAATGATAAAAGAGGCTCAAAAGAATGCAATCCAAATTGTACAACAA
GCTGTAAAGTATGTGGGGCAGTTAGCAGTTCTGAAAGGGAGCAAGCTACATTTTCTAGAA
AACGGTAACAATAAAGCTGCCAGTGTCCAGGAGGAATTCATGGATGCTGTTTGTGATGGT
GTAGGCTTAGGAATGAAGATTTTATTAGATTCTGGACTGGAAAAAGCAAAAGAACTTCAG
CATGAACTCTTTAGGCAGTGTACAAAAAATGAGGTTACCAAAGAAATGAAAACTAATGCC
ATGGGATTGATTAGATCTCTTGAAAACATCTTTGCTGAATCGAAAATTAAAAGTTTCAGA
AGGCAAGTACAAGAAGAAAACTTTGAATACCAAGATTTCAAGAGGATGGTTAATCGTGCT
CCAGAATTCTTAAAGTTAAAACATTGCTTAGAGAAAGCTATTGAAATTATTATTTCTGCA
CTGAAAGGATGCCATAGTGATATAAATCTTCTCCAGACTTGTGTTGAAAGTATTCGCAAC
TTGGCCAGTGATTTTTACAGTGACTTCAGTGTGCCTTCTACTTCTGTTGGCAGCTATGAG
AGTAGAGTAACTCACATTGTCCACCAGGAACTAGAATCTCTAGCTAAGTCTCTCCTCTTT
TGTTTTGAATCTGAAGAAAGCCCTGATTTGTTGAAACCCTGGGAAACTTATAATCAAAAT
ACCAAAGAAGAACACCAACAATCTAAATCAAGCGGGATTGACGGCAGTAAGAATAAAGGT
GTACCAAAGCGTGTCTATGAGCTCCATGGCTCATCCGCAGCAGTGAGCTCAGAGGAATGC
ACACCCAGTAGGATTCAGTGGGTG
Protein sequence predicted based on nucleotide sequence
MSLHSTHNRNNSGDILDIPSSQNSSSLNALTHSSRLKLHLKSDMSECENDDPLLRSAGKV RDINRTYVISASRKTADMPLTPNPVGRLALQRRTTRNKESSLLVSELEDTTEKTAETRLT LQRRAKTDSAEKWKTAEIDSVKMTLNVGGETENNGVSKESRTNVRIVNNAKNSFVASSVP LDEDPQVIEMMADKKYKETFSAPSRANENVALKYSSNRPPIASLSQTEVVRSGHLTTKPT QSKLDIKVLGTGNLYHRSIGKEIAKTSNKFGSLEKRTPTKCTTEHKLTTKCSLPQLKSPA PSILKNRMSNLQVKQRPKSSFLANKQERSAENTILPEEETVVQNTSAGKDPLKVENSQVT VAVRVRPFTKREKIEKASQVVFMSGKEITVEHPDTKQVYNFIYDVSFWSFDECHPHYASQ TTVYEKLAAPLLERAFEGFNTCLFAYGQTGSGKSYTMMGFSEEPGIIPRFCEDLFSQVAR KQTQEVSYHIEMSFFEVYNEKIHDLLVCKDENGQRKQPLRVREHPVYGPYVEALSMNIVS SYADIQSWLELGNKQRATAATGMNDKSSRSHSVFTLVMTQTKTEFVEGEEHDHRITSRIN LIDLAGSERCSTAHTNGDRLKEGVSINKSLLTLGKVISALSEQANQRSVFIPYRESVLTW LLKESLGGNSKTAMIATISPAASNIEETLSTLRYANQARLIVNIAKVNEDMNAKLIRELK AEIAKLKAAQRNSRNIDPERYRLCRQEITSLRMKLHQQERDMAEMQRVWKEKFEQAEKRK LQETKELQKAGIMFQMDNHLPNLVNLNEDPQLSEMLLYMIKEGTTTVGKYKPNSSHDIQL SGVLIADDHCTIKNFGGTVSIIPVGEAKTYVNGKHILEITVLRHGDRVILGGDHYFRFNH PVEVQKGKRPSGRDTPISEGPKDFEFAKNELLMAQRSQLEAEIKEAQLKAKEEMMQGIQI AKEMAQQELSSQKAAYESKIKALEAELREESQRKKMQEINNQKANHKIEELEKAKQHLEQ EIYVNKKRLEMETLATKQALEDHSIRHARILEALETEKQKIAKEVQILQQNRNNRDKTFT VQTTWSSMKLSMMIQEANAISSKLKTYYVFGRHDISDKSSSDTSIRVRNLKLGISTFWSL EKFESKLAAMKELYESNGSNRGEDAFCDPEDEWEPDITDAPVSSLSRRRSRSLMKNRRIS GCLHDIQVHPIKNLHSSHSSGLMDKSSTIYSNSAESFLPGICKELIGSSLDFFGQSYDEE RTIADSLINSFLKIYNGLFAISKAHEEQDEESQDNLFSSDRAIQSLTIQTACAFEQLVVL MKHWLSDLLPCTNIARLEDELRQEVKKLGGYLQLFLQGCCLDISSMIKEAQKNAIQIVQQ AVKYVGQLAVLKGSKLHFLENGNNKAASVQEEFMDAVCDGVGLGMKILLDSGLEKAKELQ HELFRQCTKNEVTKEMKTNAMGLIRSLENIFAESKIKSFRRQVQEENFEYQDFKRMVNRA PEFLKLKHCLEKAIEIIISALKGCHSDINLLQTCVESIRNLASDFYSDFSVPSTSVGSYE SRVTHIVHQELESLAKSLLFCFESEESPDLLKPWETYNQNTKEEHQQSKSSGIDGSKNKG VPKRVYELHGSSAAVSSEECTPSRIQWV
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: mammalian Marker: blasticidin
Host Type: mammalian Marker: blasticidin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLX304
Synonyms:
''
Sequencing Primer:
Forward:
CMV forward
Reverse:
None
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments:
None
Size (bp):
9377
Parent Vector:
None
Properties:
Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name:
David Root
Publications:
PMID:
21706014
Title: A public genome-scale lentiviral expression library of human ORFs
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
polypurine tract
cPPT
human immunodeficiency virus type 1 central polypurine tract
3027
3042
promoter
CMV
CMV promoter
14
566
promoter
T7
T7 promoter
3690
3672
recombination site
attR1
595
719
recombination site
attR2
2282
2297
selectable marker
BlastR
Blasticidin resistance gene
8753
9148
selectable marker
AmpR
Ampicillin resistance gene
4444
5304
selectable marker
CAT
CAT/CamR Chloramphenical resistance
828
1487
selectable marker
ccdB
Control of cell death B gene
1829
2134
tag
V5
C-terminal V5 tag
2301
2342
trxn regulatory element
WPRE
woodchuck hepatitis post-transcriptional regulatory element
2386
2961
