DNASU Plasmid | PSMC1 (Homo sapiens) in pLX304

Explanation of Terms

Gene:

Gene Symbol: PSMC1

Gene Name: proteasome (prosome, macropain) 26S subunit, ATPase, 1

Original Clone ID:

ccsbBroad304_06803

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pLX304

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00438195

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1321nts

Open reading frame: 1 to 1321

Reference Sequence Alignment

ATGGGTCAAAGTCAGAGTGGTGGTCATGGTCCTGGAGGTGGCAAGAAGGATGACAAGGAC
AAGAAAAAGAAATATGAACCTCCTGTACCAACTAGAGTGGGGAAAAAGAAGAAGAAAACA
AAGGGACCAGATGCTGCCAGCAAACTGCCACTGGTGACACCTCACACTCAGTGCCGGTTA
AAATTACTGAAGTTAGAGAGAATTAAAGACTATCTTCTCATGGAGGAAGAATTCATTAGA
AATCAGGAACAAATGAAACCATTAGAAGAAAAGCAAGAGGAGGAAAGATCAAAAGTGGAT
GATCTGAGGGGGACCCCGATGTCAGTAGGAACCTTGGAAGAGATTATTGATGACAATCAT
GCCATCGTGTCTACATCTGTGGGCTCAGAACACTACGTCAGCATTCTTTCATTTGTAGAC
AAGGATCTGCTGGAACCTGGCTGCTCGGTCCTGCTCAACCACAAGGTGCATGCCGTGATA
GGGGTGCTGATGGATGACACGGATCCCCTGGTCACAGTGATGAAGGTAGAAAAGGCCCCC
CAGGAGACCTATGCAGATATTGGGGGGTTGGACAACCAAATTCAGGAAATTAAGGAATCT
GTGGAGCTTCCTCTCACCCATCCTGAATATTATGAAGAGATGGGTATAAAGCCTCCTAAG
GGGGTCATTCTCTATGGTCCACCTGGCACAGGTAAAACCTTGTTAGCCAAAGCAGTAGCA
AACCAAACCTCAGCCACTTTCTTGAGAGTGGTTGGCTCTGAACTTATTCAGAAGTACCTA
GGTGATGGGCCCAAACTCGTACGGGAATTGTTCCGAGTTGCTGAAGAACATGCACCGTCC
ATCGTGTTTATTGATGAAATTGACGCCATTGGGACAAAAAGATATGACTCCAATTCTGGT
GGTGAGAGAGAAATTCAGCGAACAATGTTGGAACTGCTGAACCAGTTGGATGGATTTGAT
TCTAGGGGAGATGTGAAAGTTATCATGGCCACAAACCGAATAGAAACTTTGGATCCAGCA
CTTATCAGACCAGGCCGCATTGACAGGAAGATTGAGTTCCCCCTGCCTGATGAAAAGACG
AAGAAGCGCATCTTTCAGATTCACACAAGCAGGATGACGCTGGCTGATGATGTAACCCTG
GACGACCTGATCATGGCTAAAGATGACCTCTCTGGTGCTGACATCAAGGCAATCTGTACA
GAAGCTGGTCTGATGGCCTTAAGAGAACGTAGAATGAAAGTAACAAATGAAGACTTCAAA
AAATCTAAAGAAAATGTTCTTTATAAGAAACAGGAAGGCACCCCTGAGGGGCTGTATCTC

Protein sequence predicted based on nucleotide sequence

MGQSQSGGHGPGGGKKDDKDKKKKYEPPVPTRVGKKKKKTKGPDAASKLPLVTPHTQCRL
KLLKLERIKDYLLMEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEIIDDNH
AIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAP
QETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVA
NQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYDSNSG
GEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKT
KKRIFQIHTSRMTLADDVTLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFK
KSKENVLYKKQEGTPEGLYL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: mammalian Marker: blasticidin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pLX304

Synonyms:

Sequencing Primer:

Forward: CMV forward

Reverse: None

Description:

with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;

Comments:

None

Size (bp):

9377

Parent Vector:

None

Properties:

Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker

Author Name:

David Root

Publications:

PMID: 21706014

Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:

Vector Sequence:

Sequence:

CCCGGGGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTAC
ATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACG
TATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTG
CCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATG
GCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTA
GTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCAC
GGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTT
TCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTAT
ATAAGCAGAGCTCTCTGGCTAACTGTCGGGATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAA
AATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACAC
AACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTAT
AATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAA
TCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGT
TGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAAT
AAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTA
TGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCA
AACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCG
CAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCG
TCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGC
CCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTT
CATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGT
GGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGC
TGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGA
AGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATC
TCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAA
ATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGG
CTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACA
GAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGAT
AAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATA
TGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAA
AAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGT
CGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTT
AATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTGGTAAGCCTA
TCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAGTAATGAGCTAGCGCTAACCGGTGGCGCGTTAAGTC
GACAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTA
CGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTC
CTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTG
GTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCG
GGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGAC
AGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAATCATCGTCCTTTCCTTGGCTG
CTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAG
CGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGAC
GAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCGTCGACTTTAAGACCAATGACTTACAAGGCAGCTGTAG
ATCTTAGCCACTTTTTAAAAGAAAAGGGGGGACTGGAAGGGCTAATTCACTCCCAACGAAGACAAGATCT
GCTTTTTGCTTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGG
AACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTG
ACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTACGTATAGTAG
TTCATGTCATCTTATTATTCAGTATTTATAACTTGCAAAGAAATGAATATCAGAGAGTGAGAGGAACTTG
TTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTT
CACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGCTCTAGCTATCCCG
CCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAA
TTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTT
TTTTGGAGGCCTAGGGACGTACCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCG
TTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTT
CGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGC
GAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTA
CACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTT
TCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCC
AAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGA
CGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGT
CTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAA
AAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTGGCACTTTTCGGGGAAATGTGCG
CGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCT
TTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGA
TCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTG
ACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGT
CACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACA
TGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCG
TGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCC
TTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGC
ACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTT
ACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTT
TGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAG
ATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGC
TACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAG
AGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCA
CCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTA
CCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCAC
ACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCC
ACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGA
GGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCG
TCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGG
TTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACC
GTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAG
CGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGC
TGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTC
ATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACA
ATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAA
AAGCTGGAGCTGCAAGCTTAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAG
TTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGAT
CGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTGAATTGCCGCATTGCA
GAGATATTGTATTTAAGTGCCTAGCTCGATACATAAACGGGTCTCTCTGGTTAGACCAGATCTGAGCCTG
GGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTA
GTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAA
TCTCTAGCAGTGGCGCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAGCTCTCTCGACGCAGG
ACTCGGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAAAATTTTGA
CTAGCGGAGGCTAGAAGGAGAGAGATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGCG
ATGGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAGTATGGGCAAGC
AGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAACATCAGAAGGCTGTAGACAAATACTGG
GACAGCTACAACCATCCCTTCAGACAGGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCT
CTATTGTGTGCATCAAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAA
AACAAAAGTAAGACCACCGCACAGCAAGCGGCCGCTGATCTTCAGACCTGGAGGAGGAGATATGAGGGAC
AATTGGAGAAGTGAATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTAGCACCCACCAAGG
CAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGAGCTTTGTTCCTTGGGTTCTTGGG
AGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCCAGACAATTATTGTCTGGT
ATAGTGCAGCAGCAGAACAATTTGCTGAGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCT
GGGGCATCAAGCAGCTCCAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACAGCTCCTGGG
GATTTGGGGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGTAATAAA
TCTCTGGAACAGATTTGGAATCACACGACCTGGATGGAGTGGGACAGAGAAATTAACAATTACACAAGCT
TAATACACTCCTTAATTGAAGAATCGCAAAACCAGCAAGAAAAGAATGAACAAGAATTATTGGAATTAGA
TAAATGGGCAAGTTTGTGGAATTGGTTTAACATAACAAATTGGCTGTGGTATATAAAATTATTCATAATG
ATAGTAGGAGGCTTGGTAGGTTTAAGAATAGTTTTTGCTGTACTTTCTATAGTGAATAGAGTTAGGCAGG
GATATTCACCATTATCGTTTCAGACCCACCTCCCAACCCCGAGGGGACCCTTGCGCCTTTTCCAAGGCAG
CCCTGGGTTTGCGCAGGGACGCGGCTGCTCTGGGCGTGGTTCCGGGAAACGCAGCGGCGCCGACCCTGGG
TCTCGCACATTCTTCACGTCCGTTCGCAGCGTCACCCGGATCTTCGCCGCTACCCTTGTGGGCCCCCCGG
CGACGCTTCCTGCTCCGCCCCTAAGTCGGGAAGGTTCCTTGCGGTTCGCGGCGTGCCGGACGTGACAAAC
GGAAGCCGCACGTCTCACTAGTACCCTCGCAGACGGACAGCGCCAGGGAGCAATGGCAGCGCGCCGACCG
CGATGGGCTGTGGCCAATAGCGGCTGCTCAGCAGGGCGCGCCGAGAGCAGCGGCCGGGAAGGGGCGGTGC
GGGAGGCGGGGTGTGGGGCGGTAGTGTGGGCCCTGTTCCTGCCCGCGCGGTGTTCCGCATTCTGCAAGCC
TCCGGAGCGCACGTCGGCAGTCGGCTCCCTCGTTGACCGAATCACCGACCTCTCTCCCCAGGGGGTACCA
CCATGGCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGCTACAATCAACAGCAT
CCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCTAGCGACGGCCGCATCTTCACTGGTGTC
AATGTATATCATTTTACTGGGGGACCTTGTGCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAG
CTGGCAACCTGACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGGTG
CCGACAGGTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTGATGGACAGCCGACG
GCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCCTGCAGCTGCAGTAGTAAGAAT
TCTAGATCTTGAGACAAATGGCAGTATTCATCCACAATTTTAAAAGAAAAGGGGGGATTGGGGGGTACAG
TGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAAACTAAAGAATTACAAAAACAAATTACA
AAAATTCAAAATTTTCGGGTTTATTACAGGGACAGCAGAGATCCACTTTGGCGCCGGCTCGAGGGGG

Vector Features:

Type

Name

Description

Start

End

polypurine tract

cPPT

human immunodeficiency virus type 1 central polypurine tract

3027

3042

promoter

CMV

CMV promoter

566

promoter

T7 promoter

3690

3672

recombination site

attR1

595

719

recombination site

attR2

2282

2297

selectable marker

BlastR

Blasticidin resistance gene

8753

9148

selectable marker

AmpR

Ampicillin resistance gene

4444

5304

selectable marker

CAT

CAT/CamR Chloramphenical resistance

828

1487

selectable marker

ccdB

Control of cell death B gene

1829

2134

tag

C-terminal V5 tag

2301

2342

trxn regulatory element

WPRE

woodchuck hepatitis post-transcriptional regulatory element

2386

2961

Gene

Gene Symbol :

PSMC1

Symbol Nomenclature :

PSMC1

SYNONYM :

P26S4; S4; p56

Designation :

26S protease regulatory subunit 4|26S proteasome AAA-ATPase subunit RPT2|proteasome 26S ATPase subunit 1|proteasome 26S subunit ATPase 1|proteasome 26S subunit, ATPase, 1

Full Nomenclature :

proteasome (prosome, macropain) 26S subunit, ATPase, 1

GENEID :

5700

GenBank Accession :

DQ894881

HGNC :

9547

MIM :

602706

Vega :

OTTHUMG00000171019

Target GenBank:

DQ894881

Reference Sequence Alignment

  

HsCD00438195      ATGGGTCAAAGTCAGAGTGGTGGTCATGGTCCTGGAGGTGGCAAGAAGGATGACAAGGAC
NM_002802.2       ATGGGTCAAAGTCAGAGTGGTGGTCATGGTCCTGGAGGTGGCAAGAAGGATGACAAGGAC
                  ************************************************************

HsCD00438195      AAGAAAAAGAAATATGAACCTCCTGTACCAACTAGAGTGGGGAAAAAGAAGAAGAAAACA
NM_002802.2       AAGAAAAAGAAATATGAACCTCCTGTACCAACTAGAGTGGGGAAAAAGAAGAAGAAAACA
                  ************************************************************

HsCD00438195      AAGGGACCAGATGCTGCCAGCAAACTGCCACTGGTGACACCTCACACTCAGTGCCGGTTA
NM_002802.2       AAGGGACCAGATGCTGCCAGCAAACTGCCACTGGTGACACCTCACACTCAGTGCCGGTTA
                  ************************************************************

HsCD00438195      AAATTACTGAAGTTAGAGAGAATTAAAGACTATCTTCTCATGGAGGAAGAATTCATTAGA
NM_002802.2       AAATTACTGAAGTTAGAGAGAATTAAAGACTATCTTCTCATGGAGGAAGAATTCATTAGA
                  ************************************************************

HsCD00438195      AATCAGGAACAAATGAAACCATTAGAAGAAAAGCAAGAGGAGGAAAGATCAAAAGTGGAT
NM_002802.2       AATCAGGAACAAATGAAACCATTAGAAGAAAAGCAAGAGGAGGAAAGATCAAAAGTGGAT
                  ************************************************************

HsCD00438195      GATCTGAGGGGGACCCCGATGTCAGTAGGAACCTTGGAAGAGATTATTGATGACAATCAT
NM_002802.2       GATCTGAGGGGGACCCCGATGTCAGTAGGAACCTTGGAAGAGATCATTGATGACAATCAT
                  ******************************************** ***************

HsCD00438195      GCCATCGTGTCTACATCTGTGGGCTCAGAACACTACGTCAGCATTCTTTCATTTGTAGAC
NM_002802.2       GCCATCGTGTCTACATCTGTGGGCTCAGAACACTACGTCAGCATTCTTTCATTTGTAGAC
                  ************************************************************

HsCD00438195      AAGGATCTGCTGGAACCTGGCTGCTCGGTCCTGCTCAACCACAAGGTGCATGCCGTGATA
NM_002802.2       AAGGATCTGCTGGAACCTGGCTGCTCGGTCCTGCTCAACCACAAGGTGCATGCCGTGATA
                  ************************************************************

HsCD00438195      GGGGTGCTGATGGATGACACGGATCCCCTGGTCACAGTGATGAAGGTAGAAAAGGCCCCC
NM_002802.2       GGGGTGCTGATGGATGACACGGATCCCCTGGTCACAGTGATGAAGGTAGAAAAGGCCCCC
                  ************************************************************

HsCD00438195      CAGGAGACCTATGCAGATATTGGGGGGTTGGACAACCAAATTCAGGAAATTAAGGAATCT
NM_002802.2       CAGGAGACCTATGCAGATATTGGGGGGTTGGACAACCAAATTCAGGAAATTAAGGAATCT
                  ************************************************************

HsCD00438195      GTGGAGCTTCCTCTCACCCATCCTGAATATTATGAAGAGATGGGTATAAAGCCTCCTAAG
NM_002802.2       GTGGAGCTTCCTCTCACCCATCCTGAATATTATGAAGAGATGGGTATAAAGCCTCCTAAG
                  ************************************************************

HsCD00438195      GGGGTCATTCTCTATGGTCCACCTGGCACAGGTAAAACCTTGTTAGCCAAAGCAGTAGCA
NM_002802.2       GGGGTCATTCTCTATGGTCCACCTGGCACAGGTAAAACCTTGTTAGCCAAAGCAGTAGCA
                  ************************************************************

HsCD00438195      AACCAAACCTCAGCCACTTTCTTGAGAGTGGTTGGCTCTGAACTTATTCAGAAGTACCTA
NM_002802.2       AACCAAACCTCAGCCACTTTCTTGAGAGTGGTTGGCTCTGAACTTATTCAGAAGTACCTA
                  ************************************************************

HsCD00438195      GGTGATGGGCCCAAACTCGTACGGGAATTGTTCCGAGTTGCTGAAGAACATGCACCGTCC
NM_002802.2       GGTGATGGGCCCAAACTCGTACGGGAATTGTTCCGAGTTGCTGAAGAACATGCACCGTCC
                  ************************************************************

HsCD00438195      ATCGTGTTTATTGATGAAATTGACGCCATTGGGACAAAAAGATATGACTCCAATTCTGGT
NM_002802.2       ATCGTGTTTATTGATGAAATTGACGCCATTGGGACAAAAAGATATGACTCCAATTCTGGT
                  ************************************************************

HsCD00438195      GGTGAGAGAGAAATTCAGCGAACAATGTTGGAACTGCTGAACCAGTTGGATGGATTTGAT
NM_002802.2       GGTGAGAGAGAAATTCAGCGAACAATGTTGGAACTGCTGAACCAGTTGGATGGATTTGAT
                  ************************************************************

HsCD00438195      TCTAGGGGAGATGTGAAAGTTATCATGGCCACAAACCGAATAGAAACTTTGGATCCAGCA
NM_002802.2       TCTAGGGGAGATGTGAAAGTTATCATGGCCACAAACCGAATAGAAACTTTGGATCCAGCA
                  ************************************************************

HsCD00438195      CTTATCAGACCAGGCCGCATTGACAGGAAGATTGAGTTCCCCCTGCCTGATGAAAAGACG
NM_002802.2       CTTATCAGACCAGGCCGCATTGACAGGAAGATTGAGTTCCCCCTGCCTGATGAAAAGACG
                  ************************************************************

HsCD00438195      AAGAAGCGCATCTTTCAGATTCACACAAGCAGGATGACGCTGGCTGATGATGTAACCCTG
NM_002802.2       AAGAAGCGCATCTTTCAGATTCACACAAGCAGGATGACGCTGGCTGATGATGTAACCCTG
                  ************************************************************

HsCD00438195      GACGACCTGATCATGGCTAAAGATGACCTCTCTGGTGCTGACATCAAGGCAATCTGTACA
NM_002802.2       GACGACCTGATCATGGCTAAAGATGACCTCTCTGGTGCTGACATCAAGGCAATCTGTACA
                  ************************************************************

HsCD00438195      GAAGCTGGTCTGATGGCCTTAAGAGAACGTAGAATGAAAGTAACAAATGAAGACTTCAAA
NM_002802.2       GAAGCTGGTCTGATGGCCTTAAGAGAACGTAGAATGAAAGTAACAAATGAAGACTTCAAA
                  ************************************************************

HsCD00438195      AAATCTAAAGAAAATGTTCTTTATAAGAAACAGGAAGGCACCCCTGAGGGGCTGTATCTC
NM_002802.2       AAATCTAAAGAAAATGTTCTTTATAAGAAACAGGAAGGCACCCCTGAGGGGCTGTATCTC
                  ************************************************************

HsCD00438195      ---
NM_002802.2       TAA

Pathways

Reactome :

Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins

Reactome :

Activation of NF-kappaB in B cells

Reactome :

Adaptive Immune System

Reactome :

Antigen processing-Cross presentation

Reactome :

Antigen processing: Ubiquitination & Proteasome degradation

Reactome :

APC/C-mediated degradation of cell cycle proteins

Reactome :

APC/C:Cdc20 mediated degradation of mitotic proteins

Reactome :

APC/C:Cdc20 mediated degradation of Securin

Reactome :

APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1

Reactome :

Apoptosis

Reactome :

Assembly of the pre-replicative complex

Reactome :

Asymmetric localization of PCP proteins

Reactome :

AUF1 (hnRNP D0) destabilizes mRNA

Reactome :

Autodegradation of Cdh1 by Cdh1:APC/C

Reactome :

Autodegradation of the E3 ubiquitin ligase COP1

Reactome :

beta-catenin independent WNT signaling

Reactome :

Cdc20:Phospho-APC/C mediated degradation of Cyclin A

Reactome :

CDK-mediated phosphorylation and removal of Cdc6

Reactome :

CDT1 association with the CDC6:ORC:origin complex

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

Cell Cycle, Mitotic

Reactome :

Class I MHC mediated antigen processing & presentation

Reactome :

Cross-presentation of soluble exogenous antigens (endosomes)

Reactome :

Cyclin A:Cdk2-associated events at S phase entry

Reactome :

Cyclin E associated events during G1/S transition

Reactome :

degradation of AXIN

Reactome :

degradation of DVL

Reactome :

Disease

Reactome :

DNA Replication

Reactome :

DNA Replication Pre-Initiation

Reactome :

Downstream signaling events of B Cell Receptor (BCR)

Reactome :

ER-Phagosome pathway

Reactome :

G1/S DNA Damage Checkpoints

Reactome :

G1/S Transition

Reactome :

Gene Expression

Reactome :

HIV Infection

Reactome :

Host Interactions of HIV factors

Reactome :

Immune System

Reactome :

M Phase

Reactome :

M/G1 Transition

Reactome :

Metabolism

Reactome :

Metabolism of amino acids and derivatives

Reactome :

Metabolism of mRNA

Reactome :

Metabolism of RNA

Reactome :

Mitotic Anaphase

Reactome :

Mitotic G1-G1/S phases

Reactome :

Mitotic M-M/G1 phases

Reactome :

Mitotic Metaphase and Anaphase

Reactome :

Orc1 removal from chromatin

Reactome :

p53-Dependent G1 DNA Damage Response

Reactome :

p53-Dependent G1/S DNA damage checkpoint

Reactome :

p53-Independent DNA Damage Response

Reactome :

p53-Independent G1/S DNA damage checkpoint

Reactome :

PCP/CE pathway

Reactome :

Regulation of activated PAK-2p34 by proteasome mediated degradation

Reactome :

Regulation of APC/C activators between G1/S and early anaphase

Reactome :

Regulation of Apoptosis

Reactome :

Regulation of DNA replication

Reactome :

Regulation of mitotic cell cycle

Reactome :

Regulation of mRNA stability by proteins that bind AU-rich elements

Reactome :

Regulation of ornithine decarboxylase (ODC)

Reactome :

Removal of licensing factors from origins

Reactome :

S Phase

Reactome :

SCF(Skp2)-mediated degradation of p27/p21

Reactome :

SCF-beta-TrCP mediated degradation of Emi1

Reactome :

Separation of Sister Chromatids

Reactome :

Signal Transduction

Reactome :

Signaling by the B Cell Receptor (BCR)

Reactome :

Signaling by Wnt

Reactome :

Stabilization of p53

Reactome :

Switching of origins to a post-replicative state

Reactome :

Synthesis of DNA

Reactome :

TCF dependent signaling in response to WNT

Reactome :

Ubiquitin Mediated Degradation of Phosphorylated Cdc25A

Reactome :

Ubiquitin-dependent degradation of Cyclin D

Reactome :

Ubiquitin-dependent degradation of Cyclin D1

Reactome :

Vif-mediated degradation of APOBEC3G

Reactome :

Vpu mediated degradation of CD4

WikiPathway :

Proteasome Degradation

Protein

SMART domain :

AAA : ATPases associated with a variety of cellular activities

HPRD :

04084

Clone

Original Clone ID :

ccsbBroad304_06803

Organism

Species Specific ID:

5700

Genome

Chromosome :

Map Location :

14q32.11

Ensembl :

ENSG00000100764

Reagents

Labome :

PSMC1-antibody