Gene:
Gene Symbol:
EIF5B
Gene Name:
eukaryotic translation initiation factor 5B
Original Clone ID:
ccsbBroad304_07463
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00438822
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGGGAAGAAACAGAAAAACAAGAGCGAAGACAGCACCAAGGATGACATTGATCTTGAT
GCCTTGGCTGCAGAAATAGAAGGAGCTGGTGCTGCCAAAGAACAGGAGCCTCAAAAGTCA
AAAGGGAAAAAGAAAAAAGAGAAAAAAAAGCAGGACTTTGATGAAGATGATATCCTGAAA
GAACTGGAAGAATTGTCTTTGGAAGCTCAAGGCATCAAAGCTGACAGAGAAACTGTTGCA
GTGAAGCCAACAGAAAACAATGAAGAGGAATTCACCTCAAAAGATAAAAAAAAGAAAGGA
CAGAAGGGCAAAAAACAGAGTTTTGATGATAATGATAGCGAAGAATTGGAAGATAAAGAT
TCAAAATCAAAAAAGACTGCAAAACCGAAAGTGGAAATGTACTCTGGGAGTGATGATGAT
GATGATTTTAACAAACTTCCTAAAAAAGCTAAAGGGAAAGCTCAAAAATCAAATAAGAAG
TGGGATGGGTCAGAGGAGGATGAGGATAACAGTAAAAAAATTAAAGAGCGTTCAAGAATA
AATTCTTCTGGTGAAAGTGGTGATGAATCAGATGAATTTTTGCAATCTAGAAAAGGACAG
AAAAAAAATCAGAAAAACAAGCCAGGTCCTAACATAGAAAGTGGGAATGAAGATGATGAC
GCCTCCTTCAAAATTAAGACAGTGGCCCAAAAGAAGGCAGAAAAGAAGGAGCGCGAGAGA
AAAAAGCGAGATGAAGAAAAAGCGAAACTGCGGAAGCTGAAAGAAAAAGAAGAGTTAGAA
ACAGGTAAAAAGGATCAGAGTAAACAAAAGGAATCTCAAAGGAAATTTGAAGAAGAAACT
GTAAAATCCAAAGTGACTGTTGATACTGGAGTAATTCCTGCCTCTGAAGAGAAAGCAGAG
ACTCCCACAGCTGCAGAAGATGACAATGAAGGAGACAAAAAGAAGAAAGATAAGAAGAAA
AAGAAAGGAGAAAAGGAAGAAAAAGAGAAAGAGAAGAAAAAAGGACCTAGCAAAGCCACT
GTTAAAGCTATGCAAGAAGCTCTGGCTAAGCTTAAAGAGGAAGAAGAAAGACAGAAGAGA
GAAGAGGAAGAACGTATAAAACGGCTTGAAGAATTAGAAGCCAAGCGTAAAGAAGAGGAA
CGATTGGAACAAGAAAAAAGAGAAAGGAAAAAGCAAAAAGAAAAAGAAAGAAAAGAACGC
TTGAAAAAAGAAGGGAAACTTTTAACTAAATCCCAGAGAGAAGCCAGAGCCAGAGCCGAA
GCTACTCTTAAACTGCTACAAGCTCAGGGTGTTGAAGTGCCATCAAAAGACTCTTTGCCA
AAGAAGAGGCCAATTTATGAAGATAAAAAGAGGAAAAAAATACCACAGCAGCTAGAAAGT
AAAGAAGTGTCTGAATCAATGGAATTATGTGCTGCTGTAGAAGTTATGGAACAAGGAGTA
CCAGAAAAGGAAGAGACACCACCTCCTGTTGAACCAGAAGAAGAAGAAGATACTGAGGAT
GCTGGATTGGATGATTGGGAAGCTATGGCCAGTGATGAGGAGACAGAAAAAGTAGAAGGA
AACACAGTTCATATAGAAGTAAAAGAAAACCCTGAAGAGGAGGAGGAGGAGGAAGAAGAG
GAAGAAGAAGATGAAGAAAGTGAAGAAGAGGAGGAAGAGGAGGGAGAAAGTGAAGGCAGT
GAAGGTGATGAGGAAGATGAAAAGGTGTCAGATGAGAAGGATTCAGGGAAGACATTAGAT
AAAAAGCCAAGTAAAGAAATGAGCTCAGATTCTGAATATGACTCTGATGATGATCGGACT
AAAGAAGAAAGGGCTTATGACAAAGCAAAACGGAGGATTGAGAAACGGCGACTTGAACAT
AGTAAAAATGTAAACACCGAAAAGCTAAGAGCCCCTATTATCTGCGTACTTGGGCATGTG
GACACAGGGAAGACAAAAATTCTAGATAAGCTCCGTCACACACATGTACAAGACGGTGAA
GCAGGTGGTATCACACAACAAATTGGGGCCACCAATGTTCCTCTTGAAGCTATTAATGAA
CAGACTAAGATGATTAAAAATTTTGATAGAGAGAATGTACGGATTCCAGGAATGCTAATT
ATTGATACTCCTGGGCATGAATCTTTCAGTAATCTGAGAAATAGAGGAAGCTCTCTTTGT
GACATTGCCATTTTAGTTGTTGATATTATGCATGGTTTGGAGCCCCAGACAATTGAGTCT
ATCAACCTTCTCAAATCTAAAAAATGTCCCTTCATTGTTGCACTCAATAAGATTGATAGG
TTATATGATTGGAAAAAGAGTCCTGACTCTGATGTGGCTGCTACTTTAAAGAAGCAGAAA
AAGAATACAAAAGATGAATTTGAGGAGCGAGCAAAGGCTATTATTGTAGAATTTGCACAG
CAGGGTTTGAATGCTGCTTTGTTTTATGAGAATAAAGATCCCCGCACTTTTGTGTCTTTG
GTACCTACCTCTGCACATACTGGTGATGGCATGGGAAGTCTGATCTACCTTCTTGTAGAG
TTAACTCAGACCATGTTGAGCAAGAGACTTGCACACTGTGAAGAGCTGAGAGCACAGGTG
ATGGAGGTTAAAGCTCTCCCGGGGATGGGCACCACTATAGATGTCATCTTGATCAATGGG
CGTTTGAAGGAAGGAGATACAATCATTGTTCCTGGAGTAGAAGGGCCCATTGTAACTCAG
ATTCGAGGCCTCCTGTTACCTCCTCCTATGAAGGAATTACGAGTGAAGAACCAGTATGAA
AAGCATAAAGAAGTAGAAGCAGCTCAGGGGGTAAAGATTCTTGGAAAAGACCTGGAGAAA
ACATTGGCTGGTTTACCCCTCCTTGTGGCTTATAAAGAAGATGAAATCCCTGTTCTTAAA
GATGAATTGATCCATGAGTTAAAGCAGACACTAAATGCTATCAAATTAGAAGAAAAAGGA
GTCTATGTCCAGGCATCTACACTGGGTTCTTTGGAAGCTCTACTGGAATTTCTGAAAACA
TCAGAAGTGCCCTATGCAGGAATTAACATTGGCCCAGTGCATAAAAAAGATGTTATGAAG
GCTTCAGTGATGTTGGAACATGACCCTCAGTATGCAGTAAATTTGGCCTTCGATGTGAGA
ATTGAACGAGATGCACAAGAAATGGCTGATAGTTTAGGAGTTAGAATTTTTAGTGCAGAA
ATTATTTATCATTTATTTGATGCCTTTACAAAATATAGACAAGACTACAAGAAACAGAAA
CAAGAAGAATTTAAGCACATAGCAGTATTTCCCTGCAAGATAAAAATCCTCCCTCAGTAC
ATTTTTAATTCTCGAGATCCGATAGTGATGGGGGTGACGGTGGAAGCAGGTCAGGTGAAA
CAGGGGACACCCATGTGTGTCCCAAGCAAAAATTTTGTTGACATCGGAATAGTAACAAGT
ATTGAAATAAACCATAAACAAGTGGATGTTGCAAAAAAAGGACAAGAAGTTTGTGTAAAA
ATAGAACCTATCCCTGGTGAGTCACCCAAAATGTTTGGAAGACATTTTGAAGCTACAGAT
ATTCTTGTTAGTAAGATCAGCCGGCAGTCCATTGATGCACTCAAAGACTGGTTCAGAGAT
GAAATGCAGAAGAGTGACTGGCAGCTTATTGTGGAGCTGAAGAAAGTATTTGAAATCATC
Protein sequence predicted based on nucleotide sequence
MGKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKKQDFDEDDILK ELEELSLEAQGIKADRETVAVKPTENNEEEFTSKDKKKKGQKGKKQSFDDNDSEELEDKD SKSKKTAKPKVEMYSGSDDDDDFNKLPKKAKGKAQKSNKKWDGSEEDEDNSKKIKERSRI NSSGESGDESDEFLQSRKGQKKNQKNKPGPNIESGNEDDDASFKIKTVAQKKAEKKERER KKRDEEKAKLRKLKEKEELETGKKDQSKQKESQRKFEEETVKSKVTVDTGVIPASEEKAE TPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKR EEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAE ATLKLLQAQGVEVPSKDSLPKKRPIYEDKKRKKIPQQLESKEVSESMELCAAVEVMEQGV PEKEETPPPVEPEEEEDTEDAGLDDWEAMASDEETEKVEGNTVHIEVKENPEEEEEEEEE EEEDEESEEEEEEEGESEGSEGDEEDEKVSDEKDSGKTLDKKPSKEMSSDSEYDSDDDRT KEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGE AGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLC DIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQK KNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVE LTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQ IRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLK DELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMK ASVMLEHDPQYAVNLAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQK QEEFKHIAVFPCKIKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTS IEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRD EMQKSDWQLIVELKKVFEII
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: mammalian Marker: blasticidin
Host Type: mammalian Marker: blasticidin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLX304
Synonyms:
''
Sequencing Primer:
Forward:
CMV forward
Reverse:
None
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments:
None
Size (bp):
9377
Parent Vector:
None
Properties:
Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name:
David Root
Publications:
PMID:
21706014
Title: A public genome-scale lentiviral expression library of human ORFs
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
polypurine tract
cPPT
human immunodeficiency virus type 1 central polypurine tract
3027
3042
promoter
CMV
CMV promoter
14
566
promoter
T7
T7 promoter
3690
3672
recombination site
attR1
595
719
recombination site
attR2
2282
2297
selectable marker
BlastR
Blasticidin resistance gene
8753
9148
selectable marker
AmpR
Ampicillin resistance gene
4444
5304
selectable marker
CAT
CAT/CamR Chloramphenical resistance
828
1487
selectable marker
ccdB
Control of cell death B gene
1829
2134
tag
V5
C-terminal V5 tag
2301
2342
trxn regulatory element
WPRE
woodchuck hepatitis post-transcriptional regulatory element
2386
2961
