Gene:
Gene Symbol:
PRF1
Gene Name:
perforin 1 (pore forming protein)
Original Clone ID:
ccsbBroad304_13928
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00438969
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCAGCCCGTTGCTCCTCCTGGGCATCCTTCTCCTGCTGCTGCCCCTGCCCGTCCCTG
CCCCGTGCCACACAGCCGCACGCTCAGAGTGCAAGCGCAGCCACAAGTTCGTGCCTGGTG
CATGGCTGGCCGGGGAGGGTGTGGACGTGACCAGCCTCCGCCGCTCGGGCTCCTTCCCAG
TGGACACACAAAGGTTCCTGCGGCCCGACGGCACCTGCACCCTCTGTGAAAATGCCCTAC
AGGAGGGCACCCTCCAGCGCCTGCCTCTGGCGCTCACCAACTGGCGGGCCCAGGGCTCTG
GCTGCCAGCGCCATGTAACCAGGGCCAAAGTCAGCTCCACTGAAGCTGTGGCCCGGGATG
CGGCTCGTAGCATCCGCAACGACTGGAAGGTCGGGCTGGACGTGACTCCTAAGCCCACCA
GCAATGTGCATGTGTCTGTGGCCGGCTCACACTCACAGGCAGCCAACTTTGCAGCCCAGA
AGACCCACCAGGACCAGTACAGCTTCAGCACTGACACGGTGGAGTGCCGCTTCTACAGTT
TCCATGTGGTACACACTCCCCCGCTGCACCCTGACTTCAAGAGGGCCCTCGGGGACCTGC
CCCACCACTTCAACGCCTCCACCCAGCCCGCCTACCTCAGGCTTATCTCCAACTACGGCA
CCCACTTCATCCGGGCTGTGGAGCTGGGTGGCCGCATATCGGCCCTCACTGCCCTGCGCA
CCTGCGAGCTGGCCCTGGAAGGGCTCACGGACAACGAGGTGGAGGACTGCCTGACTGTCG
AGGCCCAGGTCAACATAGGCATCCACGGCAGCATCTCTGCCGAAGCCAAGGCCTGTGAGG
AGAAGAAGAAGAAGCACAAGATGACGGCCTCCTTCCACCAAACCTACCGGGAGCGCCATT
CGGAAGTGGTTGGCGGCCATCACACCTCCATTAACGACCTGCTGTTCGGGATCCAGGCCG
GGCCCGAGCAGTACTCAGCCTGGGTAAACTCGCTGCCCGGCAGCCCTGGCCTGGTGGACT
ACACCCTGGAACCCCTGCACGTGCTGCTGGACAGCCAGGACCCGCGGCGGGAGGCACTGA
GGAGGGCCCTGAGTCAGTACCTGACGGACAGGGCTCGCTGGAGGGACTGCAGCCGGCCGT
GCCCACCAGGGCGGCAGAAGAGCCCCCGAGACCCATGCCAGTGTGTGTGCCATGGCTCAG
CGGTCACCACCCAGGACTGCTGCCCTCGGCAGAGGGGCCTGGCCCAGCTGGAGGTGACCT
TCATCCAAGCATGGGGCCTGTGGGGGGACTGGTTCACTGCCACGGATGCCTATGTGAAGC
TCTTCTTTGGTGGCCAGGAGCTGAGGACGAGCACCGTGTGGGACAATAACAACCCCATCT
GGTCAGTGCGGCTGGATTTTGGGGATGTGCTCCTGGCCACAGGGGGGCCCCTGAGGTTGC
AGGTCTGGGATCAGGACTCTGGCAGGGACGATGACCTCCTTGGCACCTGTGATCAGGCTC
CCAAGTCTGGTTCCCATGAGGTGAGATGCAACCTGAATCATGGCCACCTAAAATTCCGCT
ATCATGCCAGGTGCTTGCCCCACCTGGGAGGAGGCACCTGCCTGGACTATGTCCCCCAAA
TGCTTCTGGGGGAGCCTCCAGGAAACCGGAGTGGGGCCGTGTGG
Protein sequence predicted based on nucleotide sequence
MAARCSSWASFSCCCPCPSLPRATQPHAQSASAATSSCLVHGWPGRVWT*PASAARAPSQ WTHKGSCGPTAPAPSVKMPYRRAPSSACLWRSPTGGPRALAASAM*PGPKSAPLKLWPGM RLVASATTGRSGWT*LLSPPAMCMCLWPAHTHRQPTLQPRRPTRTSTASALTRWSAASTV SMWYTLPRCTLTSRGPSGTCPTTSTPPPSPPTSGLSPTTAPTSSGLWSWVAAYRPSLPCA PASWPWKGSRTTRWRTA*LSRPRST*ASTAASLPKPRPVRRRRRSTR*RPPSTKPTGSAI RKWLAAITPPLTTCCSGSRPGPSSTQPG*TRCPAALAWWTTPWNPCTCCWTARTRGGRH* GGP*VST*RTGLAGGTAAGRAHQGGRRAPETHASVCAMAQRSPPRTAALGRGAWPSWR*P SSKHGACGGTGSLPRMPM*SSSLVARS*GRAPCGTITTPSGQCGWILGMCSWPQGGP*GC RSGIRTLAGTMTSLAPVIRLPSLVPMR*DAT*IMAT*NSAIMPGACPTWEEAPAWTMSPK CFWGSLQETGVGPC
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
>4 errors; >4 non-synonomous errors;
early stop codon; insertion or deletion in coding sequence
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: mammalian Marker: blasticidin
Host Type: mammalian Marker: blasticidin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLX304
Synonyms:
''
Sequencing Primer:
Forward:
CMV forward
Reverse:
None
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments:
None
Size (bp):
9377
Parent Vector:
None
Properties:
Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name:
David Root
Publications:
PMID:
21706014
Title: A public genome-scale lentiviral expression library of human ORFs
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
polypurine tract
cPPT
human immunodeficiency virus type 1 central polypurine tract
3027
3042
promoter
CMV
CMV promoter
14
566
promoter
T7
T7 promoter
3690
3672
recombination site
attR1
595
719
recombination site
attR2
2282
2297
selectable marker
BlastR
Blasticidin resistance gene
8753
9148
selectable marker
AmpR
Ampicillin resistance gene
4444
5304
selectable marker
CAT
CAT/CamR Chloramphenical resistance
828
1487
selectable marker
ccdB
Control of cell death B gene
1829
2134
tag
V5
C-terminal V5 tag
2301
2342
trxn regulatory element
WPRE
woodchuck hepatitis post-transcriptional regulatory element
2386
2961
