Gene:
Gene Symbol:
Sthe_3174
Gene Name:
Radical SAM domain-containing protein
Original Clone ID:
APC100757.102
Keyword:
None
Species:
Sphaerobacter thermophilus DSM 20745
Type:
cDNA
Vector Name:
pMCSG7
Format:
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Shonda Clancy
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
StCD00454719
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 999nts
Open reading frame: 44 to 1042
TGTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGCCACTTCCCAGCTA
TGTGCAGATCGAACCGGTCGGGCAGTGCAATCTCCGCTGCCAGATGTGCCCGATCCAGTT
CCGACGCGACGGCCCGCCCTGGGGGCCGCTCGCCTTCATGGAGTTCGACACCTTCACCCG
GATCGTCGATTCCTTCCCCGACTTGCAGACGCTCCACCTCCAGGGGCTGGGCGAGCCAAT
GATGCACCCCCGCTTCTTCGACATGGTGGCCTACGCGGCGGGGCGCGGCGCGCGGGTCAC
GACCAACAGCAACCTGACGATCCTCAACGCGCGGCGCGCCGAGCAGTGCGTCGCCAGCGG
GCTCGACTGCCTCCACGTCTCGATCGACGGCGCCAGCGCCGAGACTTACGAGCGCATCCG
GGTCCGGGCTCGCTTCGAGCGGGTAGTGCGCAACGTTGAGCTGCTGCTGGAGACCCGCGC
TCGGCTGGGTGCGACGCTGCCCGGTCTGCGGATCGTCGGCGTCGTCATGCGCCAGAACCT
GCACGAGCTGCCGGACCTGGTGCGGCTGGCGCACGGCTGGGGTGCCGAGGGGCTGTTCGT
CCAGCACCTCTGCCACGACTTCGGGGAGTCGAGCCTCCCAGAGCACTACCGGCCGATGCG
CACCTTCGTCGACGAGCAGACGCTGCTGCACGAGGACCCGGCGCGCGTGGCCGCCGCTTT
CGCCGAGGCACGCGAAGTCGCGGCAGACCTGGGGCTGGACCTGCGGCTGCCCCGCGCCGA
GCCGCGTGCCCACCCGCCGGGCACGCCCGGCCGCAAGCGCTGCTCCTGGCCCTGGACCGG
CGCCTACTTCAGCTACGAGGGCTACACCATGCCCTGCTGCATGGTGGCGACCCCCGACCG
GATCAACTTCGGCAACGCCGCCGAGCAGGGCGTCACCACCGTCTGGGGCAGCGATGAGTT
CACCGACTTCCGCGCCCGGCTCGACTCCGACGACCCGCCGGAGATCTGCCGCTCCTGCGC
GGTGTACAACGGGATCTTCTAACATTGGAAGTGGATAATAA
Protein sequence predicted based on nucleotide sequence
MPLPSYVQIEPVGQCNLRCQMCPIQFRRDGPPWGPLAFMEFDTFTRIVDSFPDLQTLHLQ GLGEPMMHPRFFDMVAYAAGRGARVTTNSNLTILNARRAEQCVASGLDCLHVSIDGASAE TYERIRVRARFERVVRNVELLLETRARLGATLPGLRIVGVVMRQNLHELPDLVRLAHGWG AEGLFVQHLCHDFGESSLPEHYRPMRTFVDEQTLLHEDPARVAAAFAEAREVAADLGLDL RLPRAEPRAHPPGTPGRKRCSWPWTGAYFSYEGYTMPCCMVATPDRINFGNAAEQGVTTV WGSDEFTDFRARLDSDDPPEICRSCAVYNGIF*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a905g,D302G
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in MCSG-APC100757
Recommended expression in:
Not Applicable
Vector Name:
pMCSG7
Synonyms:
pMCSG-7
Sequencing Primer:
Forward:
T7short
Reverse:
T7 terminator
Description:
with tag/fusion/marker assay, ligation independent cloning SspI (LIC-SspI), bacterial expression, in vitro transcription expression with AmpR and His and LIC and ori and T7 and T7 term and TEV and His 2 and LacI and lac operator; ampicillin resistance in bacterial;
Comments:
pET21 derivative; for routine HTP purification; SspI site used for LIC cloning
Size (bp):
5286
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
ligation-independent cloning (LIC) region, SspI site used
204
235
bacterial origin
ori
ColE1 pBR322-type bacterial origin of replication
3280
3280
promoter
T7
T7 promoter
360
376
protease cleavage site
TEV
TEV cleavage site cds
223
243
repressor binding site
lac operator
LacO fragment
335
357
repressor protein gene
LacI
LacI coding region
890
1849
selectable marker
AmpR
ampicillin resistance gene
4038
4898
tag
His 2
N-terminal 6xHis tag
268
285
tag
His
C-terminal 6xHis tag
140
157
trxn termination sequence
T7 term
T7 terminator sequence
26
72
