Gene:
Gene Symbol:
Taci_0145
Gene Name:
biotin and thiamin synthesis associated protein
Original Clone ID:
APC100796.102
Keyword:
None
Species:
Thermanaerovibrio acidaminovorans DSM 6589
Type:
cDNA
Vector Name:
pMCSG7
Format:
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Shonda Clancy
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
TaCD00454753
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1428nts
Open reading frame: 45 to 1472
GTGTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGGGGGTTTTGAGCG
CTAGGGCATTGGATACGGCGGACTTCATAGACCATGGCAGGATAACGGAGGTTCTGGAGG
CGGCCCGTTCCCGGGCCGGGGATGTGGGGTACCTGAGGGAAGTTTTGGCCAAGGCCCGTC
AGGCCAAGGGGCTGGACCCCCAGGAGGCGGCGGCACTCCTGTCGGTTCAGGATCCCGCAA
TGGAGGATGAGATGTTCTCCCTGGCTAGGGAGGTAAAGGAGCGGATATACGGGCGCCGCA
TAGTGCTCTTCGCCCCCCTCTACGTGAGCAACCACTGTGTGAACGACTGTTCCTACTGCG
GGTACAAATGCTCCAATCGGGGATTCGAGCGGCGGCGGCTGACCATGGAGGAACTGGCGG
AGGAGGTCCGGATCCTGGAGGGCCTGGGGCACAAGAGGCTGGCGTTGGAGGCGGGGGAGG
ACCCGGTGAACTGCCCCTTGGACTACATCCTGGATTGCATAGGGACCATATACTCTCTCA
AGTTCGACAACGGCAGCATAAGGAGGGTTAACGTCAACATAGCGGCCACCACGGTGGAGG
AGTACCGGCGCCTCAAGGAGGCCGGCATAGGTACTTACGTTCTGTTCCAGGAGACCTACC
ATCGACCCACCTACCGGGCGCTTCACCCTAGGGGGCCCAAGTCGTCGTACGACTATCACA
CCACCGCCATGGACCGGGCCATGGAAGCCGGGATAGACGATGTGGGGCTTGGGGTCCTCT
ACGGCCTGTACGACCACGTGTACGACACGGTGGCCATGCTGTTCCACGCCCGGCACCTGG
AGGAGCGTTTCGGGGTGGGGCCCCACACCATATCGGTGCCAAGGCTCAGGAGGGCCCACG
GGGTGGACGTTTCCGCGTTCCAACTGGTGGACGACGAGGCCTTCAAACGCTTGGTGGCGG
TCATAAGGCTTGCGGTGCCCTACACGGGGATGATCCTGTCCACCCGGGAGGACGCGGAGT
TCCGGGACCAGGTGATCTCCCTGGGGATATCCCAGATAAGCGCCGGTTCCTGCACCGGGG
TTGGGGGTTACGCGGACCACTACGGCCGTCGGGCGGAGGACGAGAAGCCCCAATTCGAGG
TGGGGGATCACCGATCCCCCATGGAGATCATAAAGAGCCTCATGAGGGGGGGGTACATAC
CCAGCTACTGCACCGCCTGCTACCGGGAGGGGCGCACCGGGGACCGGTTCATGAGCCTGG
CCAAGTCGGGGCAGATAGGCAACGTGTGCCAGCCCAACGCCCTGCTTACCTTGAAGGAGT
TCCTCCTGGACTACGGTGATGAGGAGGCCAGGGAGATCGGAGAGCGGCTCATCGAGAGGG
AGGTCAATGAGATCCCCCATCCCAGGGCTCGTGAGGCTGCGCTGGAGATGCTTCGCCGGG
TGGAGTCCGGCGATCGGGATCTCCGGTTCTAACATTGGAAGTGGATAACGGATCCGAATT
CGAGCTCCGTCG
Protein sequence predicted based on nucleotide sequence
MGVLSARALDTADFIDHGRITEVLEAARSRAGDVGYLREVLAKARQAKGLDPQEAAALLS VQDPAMEDEMFSLAREVKERIYGRRIVLFAPLYVSNHCVNDCSYCGYKCSNRGFERRRLT MEELAEEVRILEGLGHKRLALEAGEDPVNCPLDYILDCIGTIYSLKFDNGSIRRVNVNIA ATTVEEYRRLKEAGIGTYVLFQETYHRPTYRALHPRGPKSSYDYHTTAMDRAMEAGIDDV GLGVLYGLYDHVYDTVAMLFHARHLEERFGVGPHTISVPRLRRAHGVDVSAFQLVDDEAF KRLVAVIRLAVPYTGMILSTREDAEFRDQVISLGISQISAGSCTGVGGYADHYGRRAEDE KPQFEVGDHRSPMEIIKSLMRGGYIPSYCTACYREGRTGDRFMSLAKSGQIGNVCQPNAL LTLKEFLLDYGDEEAREIGERLIEREVNEIPHPRAREAALEMLRRVESGDRDLRF*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC100796
Recommended expression in:
Not Applicable
Vector Name:
pMCSG7
Synonyms:
pMCSG-7
Sequencing Primer:
Forward:
T7short
Reverse:
T7 terminator
Description:
with tag/fusion/marker assay, ligation independent cloning SspI (LIC-SspI), bacterial expression, in vitro transcription expression with AmpR and His and LIC and ori and T7 and T7 term and TEV and His 2 and LacI and lac operator; ampicillin resistance in bacterial;
Comments:
pET21 derivative; for routine HTP purification; SspI site used for LIC cloning
Size (bp):
5286
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
ligation-independent cloning (LIC) region, SspI site used
204
235
bacterial origin
ori
ColE1 pBR322-type bacterial origin of replication
3280
3280
promoter
T7
T7 promoter
360
376
protease cleavage site
TEV
TEV cleavage site cds
223
243
repressor binding site
lac operator
LacO fragment
335
357
repressor protein gene
LacI
LacI coding region
890
1849
selectable marker
AmpR
ampicillin resistance gene
4038
4898
tag
His 2
N-terminal 6xHis tag
268
285
tag
His
C-terminal 6xHis tag
140
157
trxn termination sequence
T7 term
T7 terminator sequence
26
72
