Gene:
Gene Symbol:
CYFIP2
Gene Name:
cytoplasmic FMR1 interacting protein 2
Original Clone ID:
FLH195938.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00045545
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGACCACGCACGTCACCCTGGAAGATGCCCTGTCCAACGTGGACCTGCTTGAAGAGCTT
CCCCTCCCCGACCAGCAGCCATGCATCGAGCCTCCACCTTCCTCCATCATGTACCAGGCT
AACTTTGACACAAACTTTGAGGACAGGAATGCATTTGTCACGGGCATTGCAAGGTACATT
GAGCAGGCTACAGTCCACTCCAGCATGAATGAGATGCTGGAGGAAGGACATGAGTATGCG
GTCATGCTGTACACCTGGCGCAGCTGTTCCCGGGCCATTCCCCAGGTGAAATGCAACGAG
CAGCCCAACCGAGTAGAGATCTATGAGAAGACAGTAGAGGTGCTGGAGCCGGAGGTCACC
AAGCTCATGAAGTTCATGTATTTTCAGCGCAAGGCCATCGAGCGGTTCTGCAGCGAGGTG
AAGCGGCTGTGCCATGCCGAGCGCAGGAAGGACTTTGTCTCTGAGGCCTACCTCCTGACC
CTTGGCAAGTTCATCAACATGTTTGCTGTCCTGGATGAGCTAAAGAACATGAAGTGCAGC
GTCAAGAATGACCACTCTGCCTACAAGAGGGCAGCACAGTTCCTGCGGAAGATGGCAGAT
CCCCAGTCTATCCAGGAGTCGCAGAACCTTTCCATGTTCCTGGCCAACCACAACAGGATC
ACCCAGTGTCTCCACCAGCAACTTGAAGTGATCCCAGGCTATGAGGAGCTGCTGGCTGAC
ATTGTCAACATCTGTGTGGATTACTACGAGAACAAGATGTACCTGACTCCCAGTGAGAAA
CATATGCTCCTCAAGGTGATGGGCTTTGGCCTCTACCTAATGGATGGAAATGTCAGTAAC
ATTTACAAACTGGATGCCAAGAAGAGAATTAATCTTAGCAAAATTGATAAATTCTTTAAG
CAGCTGCAGGTGGTGCCCCTTTTCGGCGACATGCAGATAGAGCTGGCCAGATACATTAAG
ACCAGTGCTCACTATGAAGAGAACAAGTCCAAGTGGACGTGCACCCAGAGCAGCATCAGC
CCCCAGTACAATATCTGCGAGCAGATGGTTCAGATCCGGGATGACCACATCCGCTTCATC
TCCGAGCTCGCTCGCTACAGCAACAGTGAGGTGGTGACGGGCTCAGGGCTGGACAGCCAG
AAGTCAGACGAGGAGTATCGCGAGCTCTTCGACCTAGCCCTGCGGGGTCTGCAGCTTCTA
TCCAAGTGGAGCGCCCACGTCATGGAGGTGTACTCTTGGAAGCTGGTTCATCCCACAGAC
AAGTTCTGCAACAAGGACTGTCCTGGCACCGCGGAGGAATATGAGAGAGCCACACGCTAC
AATTACACCAGTGAGGAAAAATTTGCCTTCGTTGAGGTGATCGCCATGATCAAAGGCCTG
CAGGTGCTCATGGGCAGGATGGAGAGCGTCTTCAACCAGGCCATCAGGAACACCATCTAC
GCGGCATTGCAGGACTTCGCCCAGGTGACGCTGCGTGAGCCCCTGCGGCAGGCGGTACGG
AAGAAGAAGAATGTCCTCATCAGCGTCCTACAGGCAATTCGAAAGACCATCTGTGACTGG
GAGGGAGGGCGAGAGCCCCCTAATGACCCATGCTTGAGAGGGGAGAAGGACCCCAAAGGT
GGATTTGATATCAAGGTGCCCCGGCGTGCTGTGGGGCCATCCAGCACACAGCTGTACATG
GTGCGGACCATGCTTGAATCACTCATTGCAGACAAAAGCGGCTCCAAGAAGACCCTGAGG
AGCAGCCTGGATGGACCCATTGTCCTCGCCATAGAGGACTTTCACAAACAGTCCTTCTTC
TTCACACATCTGCTCAACATCAGTGAAGCCCTGCAGCAGTGTTGTGACCTCTCCCAGCTC
TGGTTCCGAGAATTCTTCCTGGAGTTAACCATGGGCCGACGAATCCAGTTCCCCATCGAG
ATGTCCATGCCCTGGATTCTAACGGACCATATCCTGGAAACCAAAGAACCTTCCATGATG
GAGTATGTCCTCTACCCTCTGGATCTGTACAACGACAGCGCCTACTATGCTCTGACCAAG
TTTAAAAAGCAGTTCCTGTACGATGAGATAGAAGCTGAGGTGAACCTGTGTTTTGATCAG
TTTGTCTACAAGCTGGCAGACCAGATCTTTGCTTACTACAAAGCCATGGCTGGCAGTGTC
CTGTTGGATAAACGTTTTCGAGCTGAGTGTAAGAATTATGGCGTCATCATTCCGTATCCA
CCGTCCAATCGCTATGAAACACTGCTGAAGCAGAGACACGTCCAGCTGTTGGGTAGATCA
ATTGACTTGAACAGACTCATTACCCAGCGCACCTCTGCCGCCATGTATAAATCCTTGGAC
CAAGCTATCAGCCGCTTTGAGAGTGAGGACCTGACCTCCATTGTGGAGCTGGAGTGGCTG
CTGGAGATTAACCGGCTCACGCATCGGCTGCTCTGTAAGCATATGACGCTGGACAGCTTC
GATGCCATGTTCCGAGAGGCCAATCACAATGTGTCCGCCCCCTATGGCCGTATCACCCTG
CATGTCTTCTGGGAACTGAACTTTGACTTTCTCCCCAACTACTGCTACAATGGGTCCACT
AACCGTTTTGTGCGGACTGCCATTCCTTTCACCCAAGAACCACAACGAGACAAACCTGCC
AACGTCCAGCCTTATTACCTCTATGGATCCAAGCCTCTCAACATTGCCTACAGCCACATC
TACAGCTCCTACAGGAATTTCGTGGGGCCACCTCATTTCAAGACTATCTGCAGACTCCTG
GGTTATCAGGGCATCGCTGTGGTCATGGAGGAACTGCTAAAGATTGTGAAGAGCTTGCTC
CAAGGAACCATTCTCCAGTATGTGAAAACACTGATAGAGGTGATGCCCAAGATATGCCGC
TTGCCCCGACATGAGTATGGCTCCCCAGGGATCCTGGAGTTCTTCCACCACCAGCTGAAG
GACATCATTGAGTACGCAGAGCTCAAAACAGACGTGTTCCAGAGCCTGAGGGAAGTGGGC
AATGCCATCCTCTTCTGCCTCCTCATAGAGCAAGCTCTGTCTCAGGAGGAGGTCTGCGAT
TTGCTCCATGCCGCACCCTTCCAAAACATCTTGCCTAGAGTCTACATCAAAGAGGGGGAG
CGCCTGGAGGTCCGGATGAAACGTCTGGAAGCCAAGTATGCCCCGCTCCACCTGGTCCCT
CTGATCGAGCGGCTGGGGACCCCTCAGCAAATCGCCATTGCTCGCGAGGGTGACCTCCTG
ACCAAGGAGCGGCTGTGCTGTGGCCTGTCCATGTTCGAGGTCATCCTGACCCGCATTCGG
AGCTACCTGCAGGACCCCATCTGGCGGGGCCCACCGCCCACCAATGGCGTCATGCACGTC
GATGAGTGTGTGGAGTTCCACCGGCTGTGGAGCGCCATGCAGTTCGTGTACTGCATCCCT
GTGGGAACCAACGAGTTCACAGCTGAGCAGTGTTTCGGCGATGGCTTGAACTGGGCTGGT
TGCTCCATCATTGTCCTGCTGGGCCAGCAGCGTCGCTTTGACCTGTTCGACTTCTGTTAC
CACCTGCTAAAAGTGCAGAGGCAGGACGGGAAGGATGAAATCATTAAGAATGTGCCCCTG
AAGAAGATGGCCGACCGGATCAGGAAGTATCAGATCTTGAACAATGAGGTTTTTGCCATC
CTGAACAAATACATGAAGTCCGTGGAGACAGACAGTTCCACTGTGGAGCATGTGCGCTGC
TTCCAGCCACCCATCCACCAGTCCTTGGCCACCACTTGCTAG
Protein sequence predicted based on nucleotide sequence
MTTHVTLEDALSNVDLLEELPLPDQQPCIEPPPSSIMYQANFDTNFEDRNAFVTGIARYI EQATVHSSMNEMLEEGHEYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVT KLMKFMYFQRKAIERFCSEVKRLCHAERRKDFVSEAYLLTLGKFINMFAVLDELKNMKCS VKNDHSAYKRAAQFLRKMADPQSIQESQNLSMFLANHNRITQCLHQQLEVIPGYEELLAD IVNICVDYYENKMYLTPSEKHMLLKVMGFGLYLMDGNVSNIYKLDAKKRINLSKIDKFFK QLQVVPLFGDMQIELARYIKTSAHYEENKSKWTCTQSSISPQYNICEQMVQIRDDHIRFI SELARYSNSEVVTGSGLDSQKSDEEYRELFDLALRGLQLLSKWSAHVMEVYSWKLVHPTD KFCNKDCPGTAEEYERATRYNYTSEEKFAFVEVIAMIKGLQVLMGRMESVFNQAIRNTIY AALQDFAQVTLREPLRQAVRKKKNVLISVLQAIRKTICDWEGGREPPNDPCLRGEKDPKG GFDIKVPRRAVGPSSTQLYMVRTMLESLIADKSGSKKTLRSSLDGPIVLAIEDFHKQSFF FTHLLNISEALQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEPSMM EYVLYPLDLYNDSAYYALTKFKKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKAMAGSV LLDKRFRAECKNYGVIIPYPPSNRYETLLKQRHVQLLGRSIDLNRLITQRTSAAMYKSLD QAISRFESEDLTSIVELEWLLEINRLTHRLLCKHMTLDSFDAMFREANHNVSAPYGRITL HVFWELNFDFLPNYCYNGSTNRFVRTAIPFTQEPQRDKPANVQPYYLYGSKPLNIAYSHI YSSYRNFVGPPHFKTICRLLGYQGIAVVMEELLKIVKSLLQGTILQYVKTLIEVMPKICR LPRHEYGSPGILEFFHHQLKDIIEYAELKTDVFQSLREVGNAILFCLLIEQALSQEEVCD LLHAAPFQNILPRVYIKEGERLEVRMKRLEAKYAPLHLVPLIERLGTPQQIAIAREGDLL TKERLCCGLSMFEVILTRIRSYLQDPIWRGPPPTNGVMHVDECVEFHRLWSAMQFVYCIP VGTNEFTAEQCFGDGLNWAGCSIIVLLGQQRRFDLFDFCYHLLKVQRQDGKDEIIKNVPL KKMADRIRKYQILNNEVFAILNKYMKSVETDSSTVEHVRCFQPPIHQSLATTC*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
266
End on reference sequence
4027
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
