DNASU Plasmid | PTPN9 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: PTPN9

Gene Name: protein tyrosine phosphatase, non-receptor type 9

Original Clone ID:

ccsbBroadEn_11074

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC071574.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00505393

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1750nts

Open reading frame: 1 to 1750

Reference Sequence Alignment

ATGGCGCCGGAGCTGACCCCGGAGGAGGAGCAGGCTACCAAGCAGTTTCTCGAAGAGATT
AACAAGTGGACAGTTCAGTACAATGTTTCCCCGCTGTCTTGGAATGTGGCTGTCAAGTTC
CTCATGGCAAGGAAGTTTGATGTGCTCCGTGCCATAGAATTGTTCCACTCCTACAGAGAA
ACTCGAAGGAAGGAAGGCATTGTAAAGCTGAAACCTCATGAGGAACCTCTTCGTTCTGAG
ATCCTCAGTGGAAAATTCACCATCTTAAATGTTCGGGACCCAACAGGAGCCTCCATTGCC
CTCTTTACTGCCAGGTTGCATCATCCCCACAAGTCAGTCCAACATGTGGTACTTCAGGCT
CTGTTTTACTTGCTAGACAGAGCTGTGGATAGCTTTGAAACTCAGAGGAATGGACTGGTG
TTTATCTATGACATGTGTGGTTCTAATTATGCCGACTTTGAGCTGGATCTTGGCAAGAAA
GTCCTAAACCTGCTGAAGGGAGCATTTCCAGCTCGTTTGAAGAAGGTGCTGATTGTGGGG
GCACCCATATGGTTCCGAGTGCCCTATTCCATCATCAGTCTCCTCCTGAAGGACAAAGTC
CGGGAGAGGATTCAAATATTAAAGACATCTGAGGTCACGCAGCATCTGCCCAGGGAGTGT
CTTCCAGAAAACCTGGGTGGGTACGTCAAAATTGATCTCGCCACTTGGAATTTCCAGTTC
CTACCCCAGGTGAACGGCCACCCAGATCGCTTCGATGAGATCATCCTGTTCTCCCTCCCT
CCTGCCTTAGACTGGGACTCAGTACATGTTCCAGGTCCCCATGCTATGACCATCCAAGAG
TTGGTGGACTATGTTAATGCCAGGCAAAAGCAAGGAATCTATGAGGAATATGAAGACATT
CGTCGTGAGAACCCTGTTGGCACTTTCCACTGTTCCATGTCTCCAGGAAACCTAGAGAAA
AACCGTTATGGGGATGTACCCTGCCTGGACCAAACTAGAGTGAAGCTAACAAAGCGAAGT
GGCCATACTCAGACAGATTACATCAATGCCAGTTTCATGGATGGCTACAAGCAGAAGAAT
GCTTACATTGGCACACAAGGTCCTTTGGAGAATACCTATCGTGATTTCTGGCTCATGGTA
TGGGAGCAAAAAGTCTTGGTGATTGTCATGACCACCCGCTTTGAGGAAGGCGGCAGGAGA
AAGTGTGGCCAGTACTGGCCTTTAGAAAAAGACTCTCGGATCCGATTTGGCTTCCTCACA
GTGACCAATCTAGGCGTGGAGAACATGAATCATTATAAGAAAACAACGCTAGAAATTCAC
AACACAGAGGAACGGCAGAAACGCCAGGTGACCCACTTCCAGTTCTTGAGCTGGCCAGAC
TATGGTGTCCCTTCCTCAGCAGCTTCCCTCATTGACTTCTTGAGAGTGGTCAGAAACCAG
CAGAGTCTGGCTGTGAGCAACATGGGAGCACGCTCCAAAGGGCAGTGCCCTGAGCCACCC
ATTGTGGTCCATTGCAGTGCAGGCATTGGCAGGACAGGTACCTTCTGCTCACTGGACATC
TGCCTGGCACAGCTGGAGGAGCTTGGCACCCTTAATGTGTTCCAGACGGTGTCACGCATG
AGGACCCAGAGGGCCTTCAGCATCCAGACCCCTGAGCAGTACTATTTTTGCTACAAGGCC
ATCCTGGAGTTCGCAGAGAAGGAGGGCATGGTATCCTCTGGCCAAAACCTGCTGGCCGTG
GAGAGTCAG

Protein sequence predicted based on nucleotide sequence

MAPELTPEEEQATKQFLEEINKWTVQYNVSPLSWNVAVKFLMARKFDVLRAIELFHSYRE
TRRKEGIVKLKPHEEPLRSEILSGKFTILNVRDPTGASIALFTARLHHPHKSVQHVVLQA
LFYLLDRAVDSFETQRNGLVFIYDMCGSNYADFELDLGKKVLNLLKGAFPARLKKVLIVG
APIWFRVPYSIISLLLKDKVRERIQILKTSEVTQHLPRECLPENLGGYVKIDLATWNFQF
LPQVNGHPDRFDEIILFSLPPALDWDSVHVPGPHAMTIQELVDYVNARQKQGIYEEYEDI
RRENPVGTFHCSMSPGNLEKNRYGDVPCLDQTRVKLTKRSGHTQTDYINASFMDGYKQKN
AYIGTQGPLENTYRDFWLMVWEQKVLVIVMTTRFEEGGRRKCGQYWPLEKDSRIRFGFLT
VTNLGVENMNHYKKTTLEIHNTEERQKRQVTHFQFLSWPDYGVPSSAASLIDFLRVVRNQ
QSLAVSNMGARSKGQCPEPPIVVHCSAGIGRTGTFCSLDICLAQLEELGTLNVFQTVSRM
RTQRAFSIQTPEQYYFCYKAILEFAEKEGMVSSGQNLLAVESQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

PTPN9

Symbol Nomenclature :

PTPN9

SYNONYM :

MEG2; PTPMEG2

Designation :

PTPase MEG2|PTPase-MEG2|protein-tyrosine phosphatase MEG2|tyrosine-protein phosphatase non-receptor type 9

Full Nomenclature :

protein tyrosine phosphatase, non-receptor type 9

GENEID :

5780

GenBank Accession :

DQ890986

HGNC :

9661

MIM :

600768

Vega :

OTTHUMG00000142838

Target GenBank:

DQ890986

Reference Sequence Alignment

  

HsCD00505393      ------------------------------ATGGCGCCGGAGCTGACCCCGGAGGAGGAG
NM_002833.3       ATGGAGCCCGCGACCGCGCCCCGGCCCGACATGGCGCCGGAGCTGACCCCGGAGGAGGAG
                                                ******************************

HsCD00505393      CAGGCTACCAAGCAGTTTCTCGAAGAGATTAACAAGTGGACAGTTCAGTACAATGTTTCC
NM_002833.3       CAGGCTACCAAGCAGTTTCTCGAAGAGATTAACAAGTGGACAGTTCAGTACAATGTTTCC
                  ************************************************************

HsCD00505393      CCGCTGTCTTGGAATGTGGCTGTCAAGTTCCTCATGGCAAGGAAGTTTGATGTGCTCCGT
NM_002833.3       CCGCTGTCTTGGAATGTGGCTGTCAAGTTCCTCATGGCAAGGAAGTTTGATGTGCTCCGT
                  ************************************************************

HsCD00505393      GCCATAGAATTGTTCCACTCCTACAGAGAAACTCGAAGGAAGGAAGGCATTGTAAAGCTG
NM_002833.3       GCCATAGAATTGTTCCACTCCTACAGAGAAACTCGAAGGAAGGAAGGCATTGTAAAGCTG
                  ************************************************************

HsCD00505393      AAACCTCATGAGGAACCTCTTCGTTCTGAGATCCTCAGTGGAAAATTCACCATCTTAAAT
NM_002833.3       AAACCTCATGAGGAACCTCTTCGTTCTGAGATCCTCAGTGGAAAATTCACCATCTTAAAT
                  ************************************************************

HsCD00505393      GTTCGGGACCCAACAGGAGCCTCCATTGCCCTCTTTACTGCCAGGTTGCATCATCCCCAC
NM_002833.3       GTTCGGGACCCAACAGGAGCCTCCATTGCCCTCTTTACTGCCAGGTTGCATCATCCCCAC
                  ************************************************************

HsCD00505393      AAGTCAGTCCAACATGTGGTACTTCAGGCTCTGTTTTACTTGCTAGACAGAGCTGTGGAT
NM_002833.3       AAGTCAGTCCAACATGTGGTACTTCAGGCTCTGTTTTACTTGCTAGACAGAGCTGTGGAT
                  ************************************************************

HsCD00505393      AGCTTTGAAACTCAGAGGAATGGACTGGTGTTTATCTATGACATGTGTGGTTCTAATTAT
NM_002833.3       AGCTTTGAAACTCAGAGGAATGGACTGGTGTTTATCTATGACATGTGTGGTTCTAATTAT
                  ************************************************************

HsCD00505393      GCCGACTTTGAGCTGGATCTTGGCAAGAAAGTCCTAAACCTGCTGAAGGGAGCATTTCCA
NM_002833.3       GCCAACTTTGAGCTGGATCTTGGCAAGAAAGTCCTAAACCTGCTGAAGGGAGCATTTCCA
                  ***.********************************************************

HsCD00505393      GCTCGTTTGAAGAAGGTGCTGATTGTGGGGGCACCCATATGGTTCCGAGTGCCCTATTCC
NM_002833.3       GCTCGTTTGAAGAAGGTGCTGATTGTGGGGGCACCCATATGGTTCCGAGTGCCCTATTCC
                  ************************************************************

HsCD00505393      ATCATCAGTCTCCTCCTGAAGGACAAAGTCCGGGAGAGGATTCAAATATTAAAGACATCT
NM_002833.3       ATCATCAGTCTCCTCCTGAAGGACAAAGTCCGGGAGAGGATTCAAATATTAAAGACATCT
                  ************************************************************

HsCD00505393      GAGGTCACGCAGCATCTGCCCAGGGAGTGTCTTCCAGAAAACCTGGGTGGGTACGTCAAA
NM_002833.3       GAGGTCACGCAGCATCTGCCCAGGGAGTGTCTTCCAGAAAACCTGGGTGGGTACGTCAAA
                  ************************************************************

HsCD00505393      ATTGATCTCGCCACTTGGAATTTCCAGTTCCTACCCCAGGTGAACGGCCACCCAGATCGC
NM_002833.3       ATTGATCTCGCCACTTGGAATTTCCAGTTCCTACCCCAGGTGAACGGCCACCCAGATCCC
                  ********************************************************** *

HsCD00505393      TTCGATGAGATCATCCTGTTCTCCCTCCCTCCTGCCTTAGACTGGGACTCAGTACATGTT
NM_002833.3       TTCGATGAGATCATCCTGTTCTCCCTCCCTCCTGCCTTAGACTGGGACTCAGTACATGTT
                  ************************************************************

HsCD00505393      CCAGGTCCCCATGCTATGACCATCCAAGAGTTGGTGGACTATGTTAATGCCAGGCAAAAG
NM_002833.3       CCAGGTCCCCATGCTATGACCATCCAAGAGTTGGTGGACTATGTTAATGCCAGGCAAAAG
                  ************************************************************

HsCD00505393      CAAGGAATCTATGAGGAATATGAAGACATTCGTCGTGAGAACCCTGTTGGCACTTTCCAC
NM_002833.3       CAAGGAATCTATGAGGAATATGAAGACATTCGTCGTGAGAACCCTGTTGGCACTTTCCAC
                  ************************************************************

HsCD00505393      TGTTCCATGTCTCCAGGAAACCTAGAGAAAAACCGTTATGGGGATGTACCCTGCCTGGAC
NM_002833.3       TGTTCCATGTCTCCAGGAAACCTAGAGAAAAACCGTTATGGGGATGTACCCTGCCTGGAC
                  ************************************************************

HsCD00505393      CAAACTAGAGTGAAGCTAACAAAGCGAAGTGGCCATACTCAGACAGATTACATCAATGCC
NM_002833.3       CAAACTAGAGTGAAGCTAACAAAGCGAAGTGGCCATACTCAGACAGATTACATCAATGCC
                  ************************************************************

HsCD00505393      AGTTTCATGGATGGCTACAAGCAGAAGAATGCTTACATTGGCACACAAGGTCCTTTGGAG
NM_002833.3       AGTTTCATGGATGGCTACAAGCAGAAGAATGCTTACATTGGCACACAAGGTCCTTTGGAG
                  ************************************************************

HsCD00505393      AATACCTATCGTGATTTCTGGCTCATGGTATGGGAGCAAAAAGTCTTGGTGATTGTCATG
NM_002833.3       AATACCTATCGTGATTTCTGGCTCATGGTATGGGAGCAAAAAGTCTTGGTGATTGTCATG
                  ************************************************************

HsCD00505393      ACCACCCGCTTTGAGGAAGGCGGCAGGAGAAAGTGTGGCCAGTACTGGCCTTTAGAAAAA
NM_002833.3       ACCACCCGCTTTGAGGAAGGCGGCAGGAGAAAGTGTGGCCAGTACTGGCCTTTAGAAAAA
                  ************************************************************

HsCD00505393      GACTCTCGGATCCGATTTGGCTTCCTCACAGTGACCAATCTAGGCGTGGAGAACATGAAT
NM_002833.3       GACTCTCGGATCCGATTTGGCTTCCTCACAGTGACCAATCTAGGCGTGGAGAACATGAAT
                  ************************************************************

HsCD00505393      CATTATAAGAAAACAACGCTAGAAATTCACAACACAGAGGAACGGCAGAAACGCCAGGTG
NM_002833.3       CATTATAAGAAAACAACGCTAGAAATTCACAACACAGAGGAACGGCAGAAACGCCAGGTG
                  ************************************************************

HsCD00505393      ACCCACTTCCAGTTCTTGAGCTGGCCAGACTATGGTGTCCCTTCCTCAGCAGCTTCCCTC
NM_002833.3       ACCCACTTCCAGTTCTTGAGCTGGCCAGACTATGGTGTCCCTTCCTCAGCAGCTTCCCTC
                  ************************************************************

HsCD00505393      ATTGACTTCTTGAGAGTGGTCAGAAACCAGCAGAGTCTGGCTGTGAGCAACATGGGAGCA
NM_002833.3       ATTGACTTCTTGAGAGTGGTCAGAAACCAGCAGAGTCTGGCTGTGAGCAACATGGGAGCA
                  ************************************************************

HsCD00505393      CGCTCCAAAGGGCAGTGCCCTGAGCCACCCATTGTGGTCCATTGCAGTGCAGGCATTGGC
NM_002833.3       CGCTCCAAAGGGCAGTGCCCTGAGCCACCCATTGTGGTCCATTGCAGTGCAGGCATTGGC
                  ************************************************************

HsCD00505393      AGGACAGGTACCTTCTGCTCACTGGACATCTGCCTGGCACAGCTGGAGGAGCTTGGCACC
NM_002833.3       AGGACAGGTACCTTCTGCTCACTGGACATCTGCCTGGCACAGCTGGAGGAGCTTGGCACC
                  ************************************************************

HsCD00505393      CTTAATGTGTTCCAGACGGTGTCACGCATGAGGACCCAGAGGGCCTTCAGCATCCAGACC
NM_002833.3       CTTAATGTGTTCCAGACGGTGTCACGCATGAGGACCCAGAGGGCCTTCAGCATCCAGACC
                  ************************************************************

HsCD00505393      CCTGAGCAGTACTATTTTTGCTACAAGGCCATCCTGGAGTTCGCAGAGAAGGAGGGCATG
NM_002833.3       CCTGAGCAGTACTATTTTTGCTACAAGGCCATCCTGGAGTTCGCAGAGAAGGAGGGCATG
                  ************************************************************

HsCD00505393      GTATCCTCTGGCCAAAACCTGCTGGCCGTGGAGAGTCAG---
NM_002833.3       GTATCCTCTGGCCAAAACCTGCTGGCCGTGGAGAGTCAGTAA
                  ***************************************