Gene:
Gene Symbol:
RFX3
Gene Name:
regulatory factor X, 3 (influences HLA class II expression)
Original Clone ID:
ccsbBroadEn_01393
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC022191.2
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505606
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCAGACATCAGAGACTGGGTCGGACACAGGCTCGACAGTGACCTTACAAACATCTGTG
GCTAGTCAAGCAGCAGTGCCTACGCAGGTGGTACAGCAAGTACCAGTACAACAACAGGTA
CAGCAGGTACAGACTGTGCAGCAGGTACAACATGTCTATCCCGCTCAGGTGCAGTATGTG
GAAGGAAGCGATACTGTCTATACCAATGGAGCAATCCGAACAACAACGTATCCTTACACA
GAGACACAGATGTACAGCCAAAATACTGGAGGGAATTACTTTGATACTCAAGGGAGTTCC
GCCCAGGTGACTACCGTGGTCTCATCCCACAGTATGGTGGGCACTGGTGGGATTCAGATG
GGCGTCACAGGAGGACAACTCATCAGCAGCTCTGGAGGAACCTATCTGATCGGCAACTCA
ATGGAGAATTCTGGTCACTCAGTGACACACACAACTCGGGCCTCCCCAGCGACAATTGAA
ATGGCGATTGAGACGCTGCAAAAGTCTGACGGTCTGTCCACTCACAGAAGCTCTCTTCTC
AACAGCCATCTCCAGTGGCTGTTGGACAATTATGAGACAGCAGAAGGAGTGAGCCTTCCC
AGAAGCACTCTGTACAACCACTACCTTCGACACTGTCAGGAACACAAACTGGACCCAGTC
AATGCTGCCTCTTTTGGAAAATTAATAAGATCAATTTTTATGGGGCTACGAACCAGGAGA
TTGGGCACTAGAGGAAACTCCAAATACCACTACTATGGGATTCGTGTCAAGCCAGATTCC
CCTCTTAATCGTCTGCAAGAAGACATGCAGTATATGGCTATGAGACAACAACCCATGCAA
CAGAAACAAAGGTACAAGCCTATGCAGAAAGTGGATGGGGTTGCAGATGGTTTCACAGGA
AGTGGTCAACAGACAGGCACATCTGTTGAGCAAACTGTAATTGCCCAAAGCCAACATCAT
CAACAGTTTTTAGATGCATCTCGAGCACTTCCAGAGTTTGGAGAAGTTGAAATCTCTTCT
CTGCCAGATGGTACTACCTTTGAGGATATCAAGTCACTGCAGAGTCTTTATAGAGAGCAC
TGTGAGGCAATATTGGACGTTGTTGTGAATCTTCAATTTAGCCTGATAGAAAAATTGTGG
CAAACATTCTGGCGCTATTCTCCCTCTACTCCAACTGATGGCACTACCATTACCGAATCG
AGCAATCTGAGTGAAATAGAAAGTCGACTTCCGAAAGCAAAGCTGATAACTCTGTGCAAA
CATGAGTCTATCCTGAAATGGATGTGTAACTGTGACCATGGGATGTACCAGGCTTTGGTG
GAGATTCTCATCCCCGACGTCCTTAGACCTATTCCTAGTGCCTTGACCCAAGCCATTCGA
AATTTTGCAAAAAGCCTTGAAGGTTGGCTTTCCAATGCCATGAACAATATTCCACAGAGA
ATGATACAAACCAAGGTTGCCGCTGTAAGTGCCTTTGCCCAGACTCTGCGAAGATACACG
TCGCTTAATCACCTGGCCCAGGCAGCTCGTGCAGTGCTTCAGAACACTTCCCAAATCAAC
CAGATGCTTAGTGACCTCAACCGTGTCGACTTTGCCAATGTCCAGGAGCAGGCTTCCTGG
GTGTGCCAGTGTGATGACAACATGGTTCAGAGACTAGAAACAGACTTCAAGATGACTCTT
CAGCAGCAGAGCACCCTGGAGCAGTGGGCTGCGTGGCTTGACAATGTGATGATGCAAGCA
CTGAAACCCTATGAAGGAAGACCCAGTTTTCCTAAAGCCGCCAGGCAGTTTCTGCTAAAA
TGGTCTTTCTACAGCTCAATGGTTATTCGGGACTTAACCTTACGCAGTGCTGCTAGCTTT
GGCTCCTTCCACCTGATCCGTCTACTCTACGACGAATATATGTTTTACTTAGTAGAACAT
CGTGTTGCTCAGGCAACAGGAGAGACTCCTATAGCAGTCATGGGCGAGTTTGGTGATTTA
AATGCCGTGTCTCCTGGAAATCTGGATAAAGATGAAGGCAGTGAAGTAGAAAGTGAAATG
GATGAAGAACTGGATGACTCTTCAGAGCCTCAAGCCAAAAGAGAGAAAACAGAGCTGAGC
CAGGCATTTCCAGTGGGCTGCATGCAGCCTGTTCTCGAGACTGGCGTGCAACCAAGCCTC
CTGAATCCAATTCACAGCGAGCACATTGTCACAAGTACTCAGACTATCAGACAGTGCAGC
GCTACAGGAAATACCTACACTGCAGTC
Protein sequence predicted based on nucleotide sequence
MQTSETGSDTGSTVTLQTSVASQAAVPTQVVQQVPVQQQVQQVQTVQQVQHVYPAQVQYV EGSDTVYTNGAIRTTTYPYTETQMYSQNTGGNYFDTQGSSAQVTTVVSSHSMVGTGGIQM GVTGGQLISSSGGTYLIGNSMENSGHSVTHTTRASPATIEMAIETLQKSDGLSTHRSSLL NSHLQWLLDNYETAEGVSLPRSTLYNHYLRHCQEHKLDPVNAASFGKLIRSIFMGLRTRR LGTRGNSKYHYYGIRVKPDSPLNRLQEDMQYMAMRQQPMQQKQRYKPMQKVDGVADGFTG SGQQTGTSVEQTVIAQSQHHQQFLDASRALPEFGEVEISSLPDGTTFEDIKSLQSLYREH CEAILDVVVNLQFSLIEKLWQTFWRYSPSTPTDGTTITESSNLSEIESRLPKAKLITLCK HESILKWMCNCDHGMYQALVEILIPDVLRPIPSALTQAIRNFAKSLEGWLSNAMNNIPQR MIQTKVAAVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASW VCQCDDNMVQRLETDFKMTLQQQSTLEQWAAWLDNVMMQALKPYEGRPSFPKAARQFLLK WSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMFYLVEHRVAQATGETPIAVMGEFGDL NAVSPGNLDKDEGSEVESEMDEELDDSSEPQAKREKTELSQAFPVGCMQPVLETGVQPSL LNPIHSEHIVTSTQTIRQCSATGNTYTAV
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
