Gene:
Gene Symbol:
AFAP1L1
Gene Name:
actin filament associated protein 1-like 1
Original Clone ID:
ccsbBroadEn_04889
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC125093.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505650
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGACCGAGGCCAGGTGCTGGAGCAGCTGCTCCCAGAGCTCACCGGGCTGCTCAGCCTC
CTGGACCACGAGTACCTCAGCGATACCACCCTGGAAAAGAAGATGGCCGTGGCCTCCATC
CTGCAGAGCCTGCAGCCCCTTCCAGCAAAGGAGGTCTCCTACCTGTATGTGAACACAGCA
GACCTCCACTCGGGGCCCAGCTTCGTGGAATCCCTCTTTGAAGAATTTGACTGTGACCTG
AGTGACCTTCGGGACATGCCAGAGGATGATGGGGAGCCCAGCAAAGGAGCCAGCCCTGAG
CTAGCCAAGAGCCCACGCCTGAGAAACGCGGCCGACCTGCCTCCACCGCTCCCCAACAAG
CCTCCCCCTGAGGACTACTATGAAGAGGCCCTTCCTCTGGGACCCGGCAAGTCGCCTGAG
TACATCAGCTCCCACAATGGCTGCAGCCCCTCACACTCGATTGTGGATGGCTACTATGAG
GACGCAGACAGCAGCTACCCTGCAACCAGGGTGAACGGCGAGCTTAAGAGCTCCTATAAT
GACTCTGACGCAATGAGCAGCTCCTATGAGTCCTACGATGAAGAGGAGGAGGAAGGGAAG
AGCCCGCAGCCCCGACACCAGTGGCCCTCAGAGGAGGCCTCCATGCACCTGGTGAGGGAA
TGCAGGATATGTGCCTTCCTGCTGCGGAAAAAGCGTTTCGGGCAGTGGGCCAAGCAGCTG
ACGGTCATCAGGGAGGACCAGCTCCTGTGTTACAAAAGCTCCAAGGATCGGCAGCCACAT
CTGAGGTTGGCACTGGATACCTGCAGCATCATCTACGTGCCCAAGGACAGCCGGCACAAG
AGGCACGAGCTGCGTTTCACCCAGGGGGCTACCGAGGTCTTGGTGCTGGCACTGCAGAGC
CGAGAGCAGGCCGAGGAGTGGCTGAAGGTCATCCGAGAAGTGAGCAAGCCAGTTGGGGGA
GCTGAGGGAGTGGAGGTCCCCAGATCCCCAGTCCTCCTGTGCAAGTTGGACCTGGACAAG
AGGCTGTCCCAAGAGAAGCAGACCTCAGATTCTGACAGCGTGGGTGTGGGTGACAACTGT
TCTACCCTTGGCCGCCGGGAGACCTGTGATCACGGCAAAGGGAAGAAGAGCAGCCTGGCA
GAACTGAAGGGCTCAATGAGCAGGGCTGCGGGCCGCAAGATCACCCGTATCATTGGCTTC
TCCAAGAAGAAGACACTGGCCGATGACCTGCAGACGTCCTCCACCGAGGAGGAGGTTCCC
TGCTGTGGCTACCTGAACGTGCTGGTGAACCAGGGCTGGAAGGAACGCTGGTGCCGCCTG
AAGTGCAACACTCTGTATTTCCACAAGGATCACATGGACCTGCGAACCCATGTGAACGCC
ATCGCCCTGCAAGGCTGTGAGGTGGCCCCGGGCTTTGGGCCCCGACACCCATTTGCCTTC
AGGATCCTGCGCAACCGGCAGGAGGTGGCCATCTTGGAGGCAAGCTGTTCAGAGGACATG
GGTCGCTGGCTCGGGCTGCTGCTGGTGGAGATGGGCTCCAGAGTCACTCCGGAGGCGCTG
CACTATGACTACGTGGATGTGGAGACCTTAACCAGCATCGTCAGTGCTGGGCGCAACTCC
TTCCTATATGCAAGATCCTGCCAGAATCAGTGGCCTGAGCCCCGAGTCTATGATGATGTT
CCTTATGAAAAGATGCAGGACGAGGAGCCCGAGCGCCCCACAGGGGCCCAGGTGAAGCGT
CACGCCTCCTCCTGCAGTGAGAAGTCCCATCGTGTGGACCCGCAGGTCAAAGTCAAACGC
CACGCCTCCAGTGCCAATCAATACAAGTATGGCAAGAACCGAGCCGAGGAGGATGCCCGG
AGGTACTTGGTAGAAAAAGAGAAGCTGGAGAAAGAGAAAGAGACGATTCGGACAGAGCTG
ATAGCACTGAGACAGGAGAAGAGGGAACTGAAGGAAGCCATTCGGAGCAGCCCAGGAGCA
AAATTAAAGGCTCTGGAAGAAGCCGTGGCCACCCTGGAAGCTCAGTGTCGGGCAAAGGAG
GAGCGCCGGATTGACCTGGAGCTGAAGCTGGTGGCTGTGAAGGAGCGCTTGCAGCAGTCC
CTGGCAGGAGGGCCAGCCCTGGGGCTCTCCGTGAGCAGCAAGCCCAAGAGTGGGGAAACT
GCAAATAAACCCCAGAACAGCGTTCCAGAGCAACCTCTCCCTGTCAACTGTGTTTCTGAG
CTGAGGAAGAGGAGCCCATCCATCGTAGCCTCCAACCAAGGAAGGGTGCTACAGAAAGCC
AAGGAATGGGAAATGAAGAAGACC
Protein sequence predicted based on nucleotide sequence
MDRGQVLEQLLPELTGLLSLLDHEYLSDTTLEKKMAVASILQSLQPLPAKEVSYLYVNTA DLHSGPSFVESLFEEFDCDLSDLRDMPEDDGEPSKGASPELAKSPRLRNAADLPPPLPNK PPPEDYYEEALPLGPGKSPEYISSHNGCSPSHSIVDGYYEDADSSYPATRVNGELKSSYN DSDAMSSSYESYDEEEEEGKSPQPRHQWPSEEASMHLVRECRICAFLLRKKRFGQWAKQL TVIREDQLLCYKSSKDRQPHLRLALDTCSIIYVPKDSRHKRHELRFTQGATEVLVLALQS REQAEEWLKVIREVSKPVGGAEGVEVPRSPVLLCKLDLDKRLSQEKQTSDSDSVGVGDNC STLGRRETCDHGKGKKSSLAELKGSMSRAAGRKITRIIGFSKKKTLADDLQTSSTEEEVP CCGYLNVLVNQGWKERWCRLKCNTLYFHKDHMDLRTHVNAIALQGCEVAPGFGPRHPFAF RILRNRQEVAILEASCSEDMGRWLGLLLVEMGSRVTPEALHYDYVDVETLTSIVSAGRNS FLYARSCQNQWPEPRVYDDVPYEKMQDEEPERPTGAQVKRHASSCSEKSHRVDPQVKVKR HASSANQYKYGKNRAEEDARRYLVEKEKLEKEKETIRTELIALRQEKRELKEAIRSSPGA KLKALEEAVATLEAQCRAKEERRIDLELKLVAVKERLQQSLAGGPALGLSVSSKPKSGET ANKPQNSVPEQPLPVNCVSELRKRSPSIVASNQGRVLQKAKEWEMKKT
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
