Gene:
Gene Symbol:
DLGAP5
Gene Name:
discs, large (Drosophila) homolog-associated protein 5
Original Clone ID:
ccsbBroadEn_02243
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC010658.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505825
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTCTTCATCACATTTTGCCAGTCGACACAGGAAGGATATAAGTACTGAAATGATTAGA
ACTAAAATTGCTCATAGGAAATCACTGTCTCAGAAAGAAAATAGACATAAGGAATACGAA
CGAAATAGACACTTTGGTTTGAAAGATGTAAACATTCCAACCTTGGAAGGTAGAATTCTT
GTTGAATTAGATGAGACATCTCAAGGGCTTGTTCCAGAAAAGACCAATGTTAAGCCAAGG
GCAATGAAAACTATTCTAGGTGATCAACGAAAACAGATGCTCCAAAAATACAAAGAAGAA
AAGCAACTTCAAAAATTGAAAGAGCAGAGAGAGAAAGCTAAACGAGGAATATTTAAAGTG
GGTCGTTATAGACCTGATATGCCTTGTTTTCTTTTATCAAACCAGAATGCTGTGAAAGCT
GAGCCAAAAAAGGCTATTCCATCTTCTGTACGGATTACAAGGTCAAAGGCCAAAGACCAA
ATGGAGCAGACTAAGATTGATAACGAGAGTGATGTTCGAGCAATCCGACCTGGTCCAAGA
CAAACTTCTGAAAAGAAAGTGTCAGACAAAGAGAAAAAAGTTGTGCAGCCTGTAATGCCC
ACGTCGTTGAGAATGACTCGATCAGCTACTCAAGCAGCAAAGCAGGTTCCCAGAACAGTC
TCATCTACCACAGCAAGAAAGCCAGTCACAAGAGCTGCTAATGAAAACGAACCAGAAGGA
AAGGTGCCAAGTAAAGGAAGACCTGCCAAAAATGTAGAAACAAAACCCGACAAGGGTATT
TCTTGTAAAGTCGATAGTGAAGAAAATACTTTGAATTCACAAACTAATGCAACAAGTGGA
ATGAATCCAGATGGAGTCTTATCAAAAATGGAAAACTTACCTGAGATAAATACTGCAAAA
ATAAAAGGGAAGAATTCCTTTGCACCTAAGGATTTTATGTTTCAGCCACTGGATGGTCTG
AAGACCTATCAAGTAACACCTATGACTCCCAGAAGTGCCAATGCTTTTTTGACACCCAGT
TACACCTGGACTCCTTTAAAAACAGAAGTTGATGAGTCTCAAGCAACAAAAGAAATTTTG
GCACAAAAATGTAAAACTTACTCTACCAAGACAATACAGCAAGATTCAAATAAATTGCCA
TGTCCTTTGGGTCCTCTAACTGTTTGGCATGAAGAACATGTTTTAAATAAAAATGAAGCT
ACTACTAAAAATTTAAATGGCCTTCCAATAAAAGAAGTCCCATCACTTGAAAGAAATGAA
GGTCGAATTGCTCAGCCCCACCATGGTGTGCCATATTTCAGAAATATCCTCCAGTCAGAA
ACTGAGAAATTAACTTCACATTGCTTCGAGTGGGACAGGAAACTTGAATTGGACATTCCA
GATGATGCTAAAGATCTTATTCGCACAGCAGTTGGTCAAACAAGACTCCTTATGAAGGAA
AGGTTTAAACAGTTTGAAGGACTGGTTGATGATTGTGAATATAAACGAGGTATAAAGGAG
ACTACCTGTACAGATCTGGATGGATTTTGGGATATGGTTAGTTTTCAGATAGAAGATGTA
ATCCACAAATTCAACAATCTGATCAAACTTGAGGAATCTGGGTGGCAAGTCAATAATAAT
ATGAATCATAATATGAACAAAAATGTCTTTAGGAAAAAAGTTGTCTCAGGTATAGCAAGT
AAACCAAAACAGGATGATGCTGGAAGAATTGCAGCGAGAAATCGCCTAGCTGCCATAAAA
AATGCAATGAGAGAGAGAATTAGGCAGGAAGAATGTGCTGAAACAGCAGTTTCTGTGATA
CCAAAGGAAGTTGATAAAATAGTGTTCGATGCTGGATTTTTCAGAGTTGAAAGTCCTGTT
AAATTATTCTCAGGACTTTCTGTCTCTTCTGAAGGCCCTTCTCAAAGACTTGGAACACCT
AAGTCTGTCAACAAAGCTGTATCTCAGAGTAGAAATGAGATGGGCATTCCACAACAAACT
ACATCACCAGAAAATGCCGGTCCTCAGAATACGAAAAGTGAACATGTGAAGAAGACTTTG
TTTTTGAGTATTCCTGAAAGCAGGAGCAGCATAGAAGATGCTCAGTGTCCTGGATTACCA
GATTTAATTGAAGAAAATCATGTTGTAAATAAGACAGACTTGAAGGTGGATTGTTTATCC
AGTGAGAGAATGAGTTTGCCTCTTCTTGCTGGTGGAGTAGCAGATGATATTAATACTAAC
AAAAAAGAAGGAATTTCAGATGTTGTGGAAGGAATGGAACTGAATTCTTCAATTACATCA
CAGGATGTTTTGATGAGTAGCCCTGAAAAAAATACAGCTTCACAAAATAGCATCTTAGAA
GAAGGGGAAACTAAAATTTCTCAGTCAGAACTATTTGATAATAAAAGTCTCACTACTGAA
TGCCACCTTCTTGATTCACCAGGTCTAAACTGCAGTAATCCATTTACTCAGCTGGAGAGG
AGACATCAAGAACATGCCAGACACATTTCTTTTGGTGGTAACCTGATTACTTTTTCACCT
CTACAACCAGGAGAATTT
Protein sequence predicted based on nucleotide sequence
MSSSHFASRHRKDISTEMIRTKIAHRKSLSQKENRHKEYERNRHFGLKDVNIPTLEGRIL VELDETSQGLVPEKTNVKPRAMKTILGDQRKQMLQKYKEEKQLQKLKEQREKAKRGIFKV GRYRPDMPCFLLSNQNAVKAEPKKAIPSSVRITRSKAKDQMEQTKIDNESDVRAIRPGPR QTSEKKVSDKEKKVVQPVMPTSLRMTRSATQAAKQVPRTVSSTTARKPVTRAANENEPEG KVPSKGRPAKNVETKPDKGISCKVDSEENTLNSQTNATSGMNPDGVLSKMENLPEINTAK IKGKNSFAPKDFMFQPLDGLKTYQVTPMTPRSANAFLTPSYTWTPLKTEVDESQATKEIL AQKCKTYSTKTIQQDSNKLPCPLGPLTVWHEEHVLNKNEATTKNLNGLPIKEVPSLERNE GRIAQPHHGVPYFRNILQSETEKLTSHCFEWDRKLELDIPDDAKDLIRTAVGQTRLLMKE RFKQFEGLVDDCEYKRGIKETTCTDLDGFWDMVSFQIEDVIHKFNNLIKLEESGWQVNNN MNHNMNKNVFRKKVVSGIASKPKQDDAGRIAARNRLAAIKNAMRERIRQEECAETAVSVI PKEVDKIVFDAGFFRVESPVKLFSGLSVSSEGPSQRLGTPKSVNKAVSQSRNEMGIPQQT TSPENAGPQNTKSEHVKKTLFLSIPESRSSIEDAQCPGLPDLIEENHVVNKTDLKVDCLS SERMSLPLLAGGVADDINTNKKEGISDVVEGMELNSSITSQDVLMSSPEKNTASQNSILE EGETKISQSELFDNKSLTTECHLLDSPGLNCSNPFTQLERRHQEHARHISFGGNLITFSP LQPGEF
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
