Gene:
Gene Symbol:
RBM12
Gene Name:
RNA binding motif protein 12
Original Clone ID:
ccsbBroadEn_07560
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC012787.2,BC013981.2
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505877
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCTGTGGTCATCCGTTTGCAAGGTCTCCCAATTGTGGCGGGGACCATGGACATTCGC
CACTTCTTCTCTGGATTGACCATTCCTGATGGGGGCGTGCATATTGTAGGGGGTGAACTG
GGTGAGGCTTTCATCGTTTTTGCCACTGATGAAGATGCAAGGCTTGGTATGATGCGCACA
GGTGGTACAATTAAAGGGTCAAAAGTAACACTATTGTTGAGTAGTAAAACGGAAATGCAG
AATATGATTGAACTGAGTCGTAGGCGTTTTGAAACTGCCAACTTAGATATACCACCAGCA
AATGCCAGTAGATCAGGACCACCACCTAGCTCAGGAATGAGTAGCAGGGTAAACTTGCCC
ACAACAGTATCCAACTTTAATAATCCATCACCCAGTGTAGTTACTGCCACCACTTCTGTT
CATGAAAGCAACAAAAACATACAGACATTTTCCACAGCCAGCGTAGGAACAGCTCCTCCA
AATATGGGGGCTTCCTTTGGGAGCCCAACGTTTAGCTCAACTGTTCCAAGCACAGCCTCT
CCAATGAACACAGTCCCGCCGCCACCAATTCCTCCAATTCCAGCGATGCCATCTCTGCCA
CCAATGCCATCCATTCCCCCAATTCCAGTTCCTCCTCCAGTACCTACATTGCCTCCTGTG
CCTCCTGTGCCCCCGATTCCCCCAGTTCCTTCTGTGCCACCCATGACCCCACTGCCACCC
ATGTCGGGCATGCCGCCCTTGAATCCGCCACCTGTGGCACCTCTACCTGCTGGAATGAAT
GGCTCTGGAGCACCTATGAATTTGAACAATAATCTGAATCCTATGTTTCTTGGTCCGTTG
AATCCTGTTAACCCTATCCAGATGAACTCTCAGAGCAGTGTGAAGCCACTCCCCATCAAC
CCTGATGATCTGTATGTCAGTGTGCATGGAATGCCCTTTTCTGCAATGGAAAATGATGTC
AGAGATTTTTTTCATGGGCTCCGTGTTGATGCAGTGCATTTGTTGAAAGATCATGTAGGT
CGAAATAATGGGAATGGATTGGTTAAGTTTCTCTCCCCTCAAGATACATTTGAAGCTTTG
AAACGAAACAGAATGCTGATGATTCAACGCTATGTGGAAGTTAGCCCTGCCACAGAAAGA
CAGTGGGTAGCTGCTGGAGGCCATATCACTTTTAAGCAAAATATGGGACCTTCTGGACAA
ACTCATCCCCCTCCTCAGACACTTCCCAGGTCAAAATCGCCCAGTGGGCAGAAAAGATCA
AGGTCAAGATCACCACATGAGGCTGGTTTTTGTGTTTACTTGAAAGGGCTACCATTTGAA
GCAGAAAACAAACATGTCATTGATTTTTTTAAAAAGCTGGATATTGTGGAAGATAGTATT
TATATAGCTTATGGACCCAATGGGAAAGCAACTGGCGAAGGCTTTGTAGAGTTCAGAAAT
GAGGCTGACTATAAGGCTGCTCTGTGTCGTCATAAACAGTACATGGGCAATCGCTTTATT
CAAGTTCATCCAATTACTAAGAAAGGTATGCTAGAAAAGATAGATATGATTCGAAAAAGA
CTGCAGAACTTCAGCTATGACCAGAGGGAAATGATACTAAATCCAGAGGGGGATGTCAAC
TCTGCCAAAGTCTGTGCCCACATAACAAATATTCCATTCAGCATTACAAAGATGGATGTT
CTTCAGTTCCTAGAAGGAATCCCAGTGGATGAAAATGCTGTACATGTTCTTGTTGATAAC
AATGGGCAAGGTCTAGGACAGGCATTGGTTCAGTTTAAAAATGAAGATGATGCACGTAAG
TCTGAACGCTTACACCGTAAAAAACTTAATGGGAGAGAAGCTTTTGTTCATGTAGTTACC
CTAGAAGATATGAGAGAGATTGAGAAAAATCCCCCTGCCCAAGGAAAAAAGGGATTAAAG
ATGCCTGTGCCAGGTAATCCTGCAGTTCCAGGAATGCCCAATGCGGGACTGCCCGGTGTG
GGACTGCCCAGTGCAGGACTTCCCGGTGCAGGCCTGCCCAGCACAGGACTGCCTGGTTCA
GCAATAACCAGTGCAGGACTGCCTGGTGCGGGAATGCCCAGTGCAGGAATACCTAGTGCA
GGAGGTGAAGAGCATGCCTTCCTGACTGTAGGATCAAAGGAAGCCAATAATGGGCCTCCA
TTTAACTTTCCTGGTAATTTTGGTGGATCAAATGCCTTTGGGCCACCAATCCCTCCTCCA
GGATTAGGAGGCGGGGCCTTTGGTGATGCTAGGCCTGGTATGCCTTCAGTTGGAAACAGT
GGTTTGCCTGGTCTAGGACTGGATGTTCCGGGTTTTGGAGGTGGACCAAACAATTTAAGT
GGGCCATCGGGATTTGGAGGGGGCCCTCAGAATTTTGGAAATGGCCCTGGTAGCTTAGGC
GGTCCCCCGGGGTTTGGAAGTGGCCCTCCTGGTCTTGGAAGTGCCCCTGGGCATTTGGGT
GGGCCACCAGCTTTTGGGCCTGGCCCCGGCCCCGGCCCCGGCCCTGGCCCAATCCATATT
GGTGGTCCCCCTGGCTTTGCATCTAGTTCTGGAAAACCAGGACCGACAGTAATTAAAGTG
CAAAACATGCCCTTTACTGTGTCTATTGATGAGATTTTAGATTTCTTTTATGGCTATCAA
GTAATCCCAGGCTCAGTGTGTTTAAAATACAATGAAAAAGGTATGCCCACAGGTGAAGCC
ATGGTGGCCTTTGAGTCTCGGGATGAAGCCACAGCTGCTGTCATTGACTTAAATGACAGG
CCTATAGGTTCAAGAAAAGTAAAACTTGTATTAGGG
Protein sequence predicted based on nucleotide sequence
MAVVIRLQGLPIVAGTMDIRHFFSGLTIPDGGVHIVGGELGEAFIVFATDEDARLGMMRT GGTIKGSKVTLLLSSKTEMQNMIELSRRRFETANLDIPPANASRSGPPPSSGMSSRVNLP TTVSNFNNPSPSVVTATTSVHESNKNIQTFSTASVGTAPPNMGASFGSPTFSSTVPSTAS PMNTVPPPPIPPIPAMPSLPPMPSIPPIPVPPPVPTLPPVPPVPPIPPVPSVPPMTPLPP MSGMPPLNPPPVAPLPAGMNGSGAPMNLNNNLNPMFLGPLNPVNPIQMNSQSSVKPLPIN PDDLYVSVHGMPFSAMENDVRDFFHGLRVDAVHLLKDHVGRNNGNGLVKFLSPQDTFEAL KRNRMLMIQRYVEVSPATERQWVAAGGHITFKQNMGPSGQTHPPPQTLPRSKSPSGQKRS RSRSPHEAGFCVYLKGLPFEAENKHVIDFFKKLDIVEDSIYIAYGPNGKATGEGFVEFRN EADYKAALCRHKQYMGNRFIQVHPITKKGMLEKIDMIRKRLQNFSYDQREMILNPEGDVN SAKVCAHITNIPFSITKMDVLQFLEGIPVDENAVHVLVDNNGQGLGQALVQFKNEDDARK SERLHRKKLNGREAFVHVVTLEDMREIEKNPPAQGKKGLKMPVPGNPAVPGMPNAGLPGV GLPSAGLPGAGLPSTGLPGSAITSAGLPGAGMPSAGIPSAGGEEHAFLTVGSKEANNGPP FNFPGNFGGSNAFGPPIPPPGLGGGAFGDARPGMPSVGNSGLPGLGLDVPGFGGGPNNLS GPSGFGGGPQNFGNGPGSLGGPPGFGSGPPGLGSAPGHLGGPPAFGPGPGPGPGPGPIHI GGPPGFASSSGKPGPTVIKVQNMPFTVSIDEILDFFYGYQVIPGSVCLKYNEKGMPTGEA MVAFESRDEATAAVIDLNDRPIGSRKVKLVLG
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
