Gene:
Gene Symbol:
RNF40
Gene Name:
ring finger protein 40, E3 ubiquitin protein ligase
Original Clone ID:
ccsbBroadEn_02248
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC006133.1,BC011769.2,BC018647.2
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505968
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTCTGGGCCAGGCAACAAACGCGCCGCCGGCGACGGGGGCTCAGGGCCCCCGGAAAAG
AAGCTGAGTCGTGAGGAGAAGACCACCACGACTCTTATCGAGCCCATTCGTCTTGGAGGC
ATCTCTTCCACGGAGGAGATGGACCTGAAGGTACTACAGTTCAAGAACAAGAAACTGGCA
GAGCGGCTGGAACAACGGCAGGCTTGTGAAGATGAACTCCGAGAACGAATTGAGAAGTTG
GAGAAGCGGCAGGCCACAGATGATGCCACACTCCTCATCGTCAATCGCTACTGGGCCCAG
CTGGATGAAACTGTGGAAGCCCTTCTCCGATGCCATGAGAGCCAGGGGGAGCTGTCTTCA
GCGCCTGAGGCACCTGGGACCCAGGAGGGGCCAACATGTGATGGGACTCCTCTCCCAGAG
CCGGGGACATCAGAGCTGAGAGACCCCTTGCTGATGCAGCTGCGGCCCCCTCTCAGTGAG
CCGGCCTTGGCTTTTGTGGTGGCACTGGGTGCCAGCAGCAGTGAGGAGGTGGAGCTGGAG
CTGCAAGGCCGAATGGAGTTCTCCAAGGCAGCTGTGTCTCGTGTGGTAGAGGCCTCAGAC
CGCCTACAGCGCCGGGTGGAGGAACTCTGTCAGCGAGTGTACAGCCGAGGGGACAGTGAG
CCCCTCAGTGAGGCGGCTCAGGCACACACCCGAGAGCTGGGCCGTGAGAACCGGCGACTG
CAGGACTTGGCCACTCAGCTGCAGGAGAAACACCACCGCATCTCATTGGAGTACTCCGAG
CTCCAGGATAAAGTGACATCGGCAGAGACCAAGGTGCTGGAGATGGAGACAACAGTGGAG
GACTTGCAGTGGGACATCGAGAAGCTGCGGAAGCGAGAGCAAAAGCTCAATAAGCACCTG
GCAGAGGCCTTAGAGCAGCTTAACTCTGGCTACTATGTATCTGGGAGCTCCTCAGGCTTC
CAGGGGGGCCAGATCACACTCAGCATGCAGAAGTTTGAGATGCTGAATGCAGAGTTAGAG
GAAAACCAGGAACTGGCCAACAGCCGTATGGCAGAGCTGGAGAAACTGCAGGCCGAACTT
CAGGGGGCTGTGCGGACCAATGAGCGCCTCAAGGTGGCCCTGCGGAGCCTTCCTGAGGAG
GTAGTGCGGGAGACGGGGGAGTACCGCATGCTGCAGGCCCAATTCTCACTGCTCTACAAC
GAGTCTCTGCAAGTGAAGACCCAGCTAGACGAGGCTCGGGGCCTGCTGCTGGCCACAAAG
AACTCCCACCTGCGACACATCGAGCACATGGAGAGCGACGAGCTGGGGCTGCAGAAGAAG
CTACGCACAGAGGTCATTCAGCTGGAGGACACGCTGGCCCAGGTACGCAAGGAGTATGAG
ATGCTGCGCATCGAGTTTGAGCAGAATCTGGCGGCCAACGAGCAGGCGGGGCCCATCAAC
CGTGAGATGCGCCACCTGATTAGTAGTCTTCAAAACCACAACCACCAGCTAAAAGGGGAC
GCCCAGCGATACAAGCGGAAGCTTCGAGAAGTACAAGCTGAGATTGGCAAGCTCCGGGCC
CAGGCCAGTGGCTCTGCCCACTCCACCCCCAACCTGGGCCACCCAGAGGATTCTGGCGTC
AGTGCCCCAGCCCCAGGGAAAGAGGAGGGTGGGCCAGGCCCTGTCAGTACCCCCGACAAC
AGAAAGGAGATGGCTCCAGTGCCTGGCACCACCACTACTACCACTTCAGTGAAGAAGGAG
GAGCTGGTCCCCTCTGAAGAGGACTTCCAGGGTATAACCCCTGGGGCCCAGGGCCCTTCC
TCCCGGGGCCGAGAACCTGAGGCCAGGCCCAAGCGGGAGCTTCGGGAACGGGAAGGTCCC
AGCCTAGGACCTCCACCTGTAGCCTCCGCTCTCTCAAGGGCTGATCGGGAGAAGGCCAAG
GTGGAAGAAACCAAGCGGAAGGAATCAGAACTCCTCAAGGGTCTCCGAGCAGAGCTCAAG
AAGGCCCAGGAGAGCCAGAAGGAGATGAAACTGCTGCTGGATATGTACAAGTCAGCGCCC
AAGGAGCAGCGGGATAAGGTGCAGCTCATGGCAGCGGAACGCAAGGCTAAGGCCGAGGTT
GATGAGCTGCGGAGCCGCATCCGGGAATTGGAGGAGAGGGATCGAAGGGAGAGCAAGAAG
ATCGCGGATGAGGATGCCCTGCGGCGCATTCGGCAGGCAGAGGAGCAGATAGAACACCTG
CAGCGCAAGCTGGGTGCCACCAAGCAGGAGGAGGAGGCTCTGCTCTCAGAGATGGATGTG
ACAGGTCAGGCTTTTGAGGACATGCAGGAACAGAACGGGCGGCTGCTACAGCAGTTGCGG
GAAAAGGATGATGCCAACTTTAAGCTAATGTCAGAGCGGATCAAGGCCAACCAGATTCAC
AAGCTGCTGCGGGAGGAGAAGGATGAGTTGGGCGAGCAGGTCCTTGGCCTCAAGTCCCAG
GTGGATGCCCAGCTGCTGACTGTGCAGAAGCTGGAGGAGAAGGAGCGAGCCTTGCAGGGC
AGCCTCGGGGGTGTGGAGAAGGAGCTGACGCTGCGCAGCCAAGCCCTGGAGCTCAACAAG
CGGAAGGCTGTAGAAGCCGCCCAGCTGGCCGAGGACCTGAAGGTGCAGCTGGAGCACGTG
CAGACTCGGCTGCGGGAGATCCAGCCCTGCCTGGCAGAGAGCCGGGCTGCTCGTGAGAAA
GAGAGCTTCAACCTCAAGAGGGCTCAGGAGGACATCTCACGGCTGCGGCGCAAGCTGGAA
AAGCAGAGGAAGGTGGAGGTCTACGCAGATGCCGACGAAATCCTCCAGGAGGAGATCAAG
GAGTACAAGGCGCGGTTGACCTGCCCCTGCTGTAACACCCGCAAGAAGGATGCAGTCCTT
ACCAAGTGCTTCCACGTTTTCTGCTTCGAGTGCGTGCGGGGCCGCTATGAGGCCCGCCAG
AGGAAGTGCCCCAAGTGCAACGCGGCCTTTGGTGCCCACGACTTCCATCGTATCTACATC
AGC
Protein sequence predicted based on nucleotide sequence
MSGPGNKRAAGDGGSGPPEKKLSREEKTTTTLIEPIRLGGISSTEEMDLKVLQFKNKKLA ERLEQRQACEDELRERIEKLEKRQATDDATLLIVNRYWAQLDETVEALLRCHESQGELSS APEAPGTQEGPTCDGTPLPEPGTSELRDPLLMQLRPPLSEPALAFVVALGASSSEEVELE LQGRMEFSKAAVSRVVEASDRLQRRVEELCQRVYSRGDSEPLSEAAQAHTRELGRENRRL QDLATQLQEKHHRISLEYSELQDKVTSAETKVLEMETTVEDLQWDIEKLRKREQKLNKHL AEALEQLNSGYYVSGSSSGFQGGQITLSMQKFEMLNAELEENQELANSRMAELEKLQAEL QGAVRTNERLKVALRSLPEEVVRETGEYRMLQAQFSLLYNESLQVKTQLDEARGLLLATK NSHLRHIEHMESDELGLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPIN REMRHLISSLQNHNHQLKGDAQRYKRKLREVQAEIGKLRAQASGSAHSTPNLGHPEDSGV SAPAPGKEEGGPGPVSTPDNRKEMAPVPGTTTTTTSVKKEELVPSEEDFQGITPGAQGPS SRGREPEARPKRELREREGPSLGPPPVASALSRADREKAKVEETKRKESELLKGLRAELK KAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKAKAEVDELRSRIRELEERDRRESKK IADEDALRRIRQAEEQIEHLQRKLGATKQEEEALLSEMDVTGQAFEDMQEQNGRLLQQLR EKDDANFKLMSERIKANQIHKLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQG SLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREK ESFNLKRAQEDISRLRRKLEKQRKVEVYADADEILQEEIKEYKARLTCPCCNTRKKDAVL TKCFHVFCFECVRGRYEARQRKCPKCNAAFGAHDFHRIYIS
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
