Gene:
Gene Symbol:
ZNF217
Gene Name:
zinc finger protein 217
Original Clone ID:
ccsbBroadEn_01820
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC113427.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00506024
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCAATCGAAAGTGACAGGAAACATGCCAACTCAATCCCTCTTAATGTACATGGATGGG
CCAGAAGTGATTGGCAGCTCTCTTGGCAGTCCGATGGAGATGGAGGATGCCTTGTCAATG
AAAGGGACCGCTGTTGTTCCATTCCGAGCTACACAAGAAAAAAATGTCATCCAAATCGAG
GGGTATATGCCCTTGGATTGCATGTTCTGCAGCCAGACCTTCACACATTCAGAAGACCTT
AATAAACATGTCTTAATGCAACACCGGCCTACCCTCTGTGAACCAGCAGTTCTTCGGGTT
GAAGCAGAGTATCTCAGTCCGCTTGATAAAAGTCAAGTGCGAACAGAACCTCCCAAGGAA
AAGAATTGCAAGGAAAATGAATTTAGCTGTGAGGTATGTGGGCAGACATTTAGAGTCGCT
TTTGATGTTGAGATCCACATGAGAACACACAAAGATTCTTTCACTTACGGGTGTAACATG
TGCGGAAGAAGATTCAAGGAGCCTTGGTTTCTTAAAAATCACATGCGGACACATAATGGC
AAATCGGGGGCCAGAAGCAAACTGCAGCAAGGCTTGGAGAGTAGTCCAGCAACGATCAAC
GAGGTCGTCCAGGTGCACGCGGCCGAGAGCATCTCCTCTCCTTACAAAATCTGCATGGTT
TGTGGCTTCCTATTTCCAAATAAAGAAAGTCTAATTGAGCACCGCAAGGTGCACACCAAA
AAAACTGCTTTCGGTACCAGCAGCGCGCAGACAGACTCTCCACAAGGAGGAATGCCGTCC
TCGAGGGAGGACTTCCTGCAGTTGTTCAACTTGAGACCAAAATCTCACCCTGAAACGGGG
AAGAAGCCTGTCAGATGCATCCCTCAGCTCGATCCGTTCACCACCTTCCAGGCTTGGCAG
CTGGCTACCAAAGGAAAAGTTGCCATTTGCCAAGAAGTGAAGGAATCGGGGCAAGAAGGG
AGCACCGACAACGACGATTCGAGTTCCGAGAAGGAGCTTGGAGAAACAAATAAGGGCAGT
TGTGCAGGCCTCTCGCAAGAGAAAGAGAAGTGCAAACACTCCCACGGCGAAGCGCCCTCC
GTGGACGCGGATCCCAAGTTACCCAGTAGCAAGGAGAAGCCCACTCACTGCTCCGAGTGC
GGCAAAGCTTTCAGAACCTACCACCAGCTGGTCTTGCACTCCAGGGTCCACAAGAAGGAC
CGGAGGGCCGGCGCGGAGTCGCCCACCATGTCTGTGGACGGGAGGCAGCCGGGGACGTGT
TCTCCTGACCTCGCCGCCCCTCTGGATGAAAATGGAGCCGTGGATCGAGGGGAAGGTGGT
TCTGAAGACGGATCTGAGGATGGGCTTCCCGAAGGAATCCATCTGGATAAAAATGATGAT
GGAGGAAAAATAAAACATCTTACATCTTCAAGAGAGTGTAGTTATTGTGGAAAGTTTTTC
CGTTCAAATTATTACCTCAATATTCATCTCAGAACGCATACAGGTGAAAAACCATACAAA
TGTGAATTTTGTGAATATGCTGCAGCCCAGAAGACATCTCTGAGGTATCACTTGGAGAGA
CATCACAAGGAAAAACAAACCGATGTTGCTGCTGAAGTCAAGAACGATGGTAAAAATCAG
GACACTGAAGATGCACTATTAACCGCTGACAGTGCGCAAACCAAAAATTTGAAAAGATTT
TTTGATGGTGCCAAAGATGTTACAGGCAGTCCACCTGCAAAGCAGCTTAAGGAGATGCCT
TCTGTTTTTCAGAATGTTCTGGGCAGCGCTGTCCTCTCACCAGCACACAAAGATACTCAG
GATTTCCATAAAAATGCAGCTGATGACAGTGCTGATAAAGTGAATAAAAACCCTACCCCT
GCTTACCTGGACCTGTTAAAAAAGAGATCAGCAGTTGAAACTCAGGCAAATAACCTCATC
TGTAGAACCAAGGCGGATGTTACTCCTCCTCCGGATGGCAGTACCACCCATAACCTTGAA
GTTAGCCCCAAAGAGAAGCAAACGGAGACCGCAGCTGACTGCAGATACAGGCCAAGTGTG
GATTGTCACGAAAAACCTTTAAATTTATCCGTGGGGGCTCTTCACAATTGCCCGGCAATT
TCTTTGAGTAAAAGTTTGATTCCAAGTATCACCTGTCCATTTTGTACCTTCAAGACATTT
TATCCAGAAGTTTTAATGATGCACCAGAGACTGGAGCATAAATACAATCCTGACGTTCAT
AAAAACTGTCGAAACAAGTCCTTGCTTAGAAGTCGACGTACCGGATGCCCGCCAGCGTTG
CTGGGAAAAGATGTGCCTCCCCTCTCTAGTTTCTGTAAACCCAAGCCCAAGTCTGCTTTC
CCGGCGCAGTCCAAATCCCTGCCATCTGCGAAGGGGAAGCAGAGCCCTCCTGGGCCAGGC
AAGGCCCCTCTGACTTCAGGGATAGACTCTAGCACTTTAGCCCCAAGTAACCTGAAGTCC
CACAGACCACAGCAGAATGTGGGGGTCCAAGGGGCCGCCACCAGGCAACAGCAATCTGAG
ATGTTTCCTAAAACCAGTGTTTCCCCTGCACCGGATAAGACAAAAAGACCCGAGACAAAA
TTGAAACCTCTTCCAGTAGCTCCTTCTCAGCCCACCCTCGGCAGCAGTAACATCAATGGT
TCCATCGACTACCCCGCCAAGAACGACAGCCCGTGGGCACCTCCGGGAAGAGACTATTTC
TGTAATCGGAGTGCCAGCAATACTGCAGCAGAATTTGGTGAGCCCCTTCCAAAAAGACTG
AAGTCCAGCGTGGTTGCCCTTGACGTTGACCAGCCCGGGGCCAATTACAGAAGAGGCTAT
GACCTTCCCAAGTACCATATGGTCAGAGGCATCACATCACTGTTACCGCAGGACTGTGTG
TATCCGTCGCAGGCGCTGCCTCCCAAACCAAGGTTCCTGAGCTCCAGCGAGGTCGATTCT
CCAAATGTGCTGACTGTTCAGAAGCCCTATGGTGGCTCCGGGCCACTTTACACTTGTGTG
CCTGCTGGTAGTCCAGCATCCAGCTCGACGTTAGAAGGAAAAAGGCCTGTGTCATATCAA
CACTTATCTAACAGCATGGCACAAAAGAGAAACTATGAGAATTTTATTGGGAATGCACAT
TATCGACCAAATGACAAAAAAACT
Protein sequence predicted based on nucleotide sequence
MQSKVTGNMPTQSLLMYMDGPEVIGSSLGSPMEMEDALSMKGTAVVPFRATQEKNVIQIE GYMPLDCMFCSQTFTHSEDLNKHVLMQHRPTLCEPAVLRVEAEYLSPLDKSQVRTEPPKE KNCKENEFSCEVCGQTFRVAFDVEIHMRTHKDSFTYGCNMCGRRFKEPWFLKNHMRTHNG KSGARSKLQQGLESSPATINEVVQVHAAESISSPYKICMVCGFLFPNKESLIEHRKVHTK KTAFGTSSAQTDSPQGGMPSSREDFLQLFNLRPKSHPETGKKPVRCIPQLDPFTTFQAWQ LATKGKVAICQEVKESGQEGSTDNDDSSSEKELGETNKGSCAGLSQEKEKCKHSHGEAPS VDADPKLPSSKEKPTHCSECGKAFRTYHQLVLHSRVHKKDRRAGAESPTMSVDGRQPGTC SPDLAAPLDENGAVDRGEGGSEDGSEDGLPEGIHLDKNDDGGKIKHLTSSRECSYCGKFF RSNYYLNIHLRTHTGEKPYKCEFCEYAAAQKTSLRYHLERHHKEKQTDVAAEVKNDGKNQ DTEDALLTADSAQTKNLKRFFDGAKDVTGSPPAKQLKEMPSVFQNVLGSAVLSPAHKDTQ DFHKNAADDSADKVNKNPTPAYLDLLKKRSAVETQANNLICRTKADVTPPPDGSTTHNLE VSPKEKQTETAADCRYRPSVDCHEKPLNLSVGALHNCPAISLSKSLIPSITCPFCTFKTF YPEVLMMHQRLEHKYNPDVHKNCRNKSLLRSRRTGCPPALLGKDVPPLSSFCKPKPKSAF PAQSKSLPSAKGKQSPPGPGKAPLTSGIDSSTLAPSNLKSHRPQQNVGVQGAATRQQQSE MFPKTSVSPAPDKTKRPETKLKPLPVAPSQPTLGSSNINGSIDYPAKNDSPWAPPGRDYF CNRSASNTAAEFGEPLPKRLKSSVVALDVDQPGANYRRGYDLPKYHMVRGITSLLPQDCV YPSQALPPKPRFLSSSEVDSPNVLTVQKPYGGSGPLYTCVPAGSPASSSTLEGKRPVSYQ HLSNSMAQKRNYENFIGNAHYRPNDKKT
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
